LOCUS       pTW335v3_[pBR322]_Ptac_ 7522 bp ds-DNA     circular     05-JUL-2024
DEFINITION  .
ACCESSION   urn.local...16e-aqel875
SOURCE      null
COMMENT     Imported using the Genbank importer. File name: pTW004c.gb [ColE1]
            Ptet T7RNAP N-term aadA. Source name: pTW002 - AP SC101 RepA Ptet
            SD8 MBP bla tetR (Note: Geneious sometimes stores the sequence name
            in the source attribute) Source name: pTW002a - CP ColEI Rop
            dnak-P1 SD8 LuxABtetA-orfL aadA (Note: Geneious sometimes stores
            the sequence name in the source attribute) Source name: pTW002a -
            CP ColEI Rop dnak-P1 SD8 LuxABtetA-orfL aadA (Note: Geneious
            sometimes stores the sequence name in the source attribute) Source
            name: pTW001b - AP SC101 RepA Ptet SD8 MBP(wt) bla (Note: Geneious
            sometimes stores the sequence name in the source attribute) Source
            name: pTW002 - AP SC101 RepA Ptet SD8 MBP bla tetR (Note: Geneious
            sometimes stores the sequence name in the source attribute) Source
            name: pTW001b - AP SC101 RepA Ptet SD8 MBP(wt) bla (Note: Geneious
            sometimes stores the sequence name in the source attribute)
FEATURES             Location/Qualifiers
     CDS             complement(699..890)
                     /label="Rop"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             complement(2202..3284)
                     /label="lacI"
                     /ApEinfo_revcolor="#7bcdc8"
                     /ApEinfo_fwdcolor="#7bcdc8"
     promoter        complement(3285..3362)
                     /label="lacI promoter"
                     /ApEinfo_revcolor="#c4df9b"
                     /ApEinfo_fwdcolor="#c4df9b"
     promoter        3605..3633
                     /label="tac promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     operator        3637..3659
                     /label="lacO"
                     /ApEinfo_revcolor="#f6989d"
                     /ApEinfo_fwdcolor="#f6989d"
     RBS             3725..3737
                     /label="RBS"
                     /ApEinfo_revcolor="#82ca9d"
                     /ApEinfo_fwdcolor="#82ca9d"
     CDS             3738..5171
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MPQTETKAGVGFQAGVKDYKLTYYTPEYETKDTDILAAFRVSPQPGVPPEEAGAAVAAESSTGTWTTVWTDGLTSLDRYKGRCYHIEPVAGEDSQWICYVAYPLDLFEEGSVTNMFTSIVGNVFGFKALRALRLEDLRIPPTYSKTFQGPPHGIQVERDKLNKYGRPLLGCTIKPKLGLSAKNYGRACYECLRGGLDFTKDDENVNSQPFMRWRDRFVFCAEAIYKSQAETGEIKGHYLNATAGTCEEMIKRAVFARELGVPIVMHDYLTGGFTANTTLAHYCRDNGLLLHIHRAMHAVIDRQKNHGMHFRVLAKALRMSGGDHIHSGTVVGKLEGEREMTLGFVDLLRDDFIEKDRARGIFFTQDWVSMPGVIPVASGGIHVWHMPALTEIFGDDSVLQFGGGTLGHPWGNAPGAAANRVALEACVQARNEGRDLAREGNEIIRAACKWSPELAAACEVWKAIKFEFEPVDTIDKKV"
     misc_feature    3738..5174
                     /label="HvRbcL"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             5172..5174
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="*"
     RBS             5187..5209
                     /label="RBS"
                     /ApEinfo_revcolor="#7bcdc8"
                     /ApEinfo_fwdcolor="#7bcdc8"
     misc_feature    5217..5627
                     /label="HvRbcS"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             5217..5627
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MCMQVWPIEGIKKFETLSYLPPLSTEALLKQVDYLIRSKWVPCLEFSKVGFIFREHNASPGYYDGRYWTMWKLPMFGCTDATQVLNEVEEVKKEYPDAYVRIIGFDNMRQVQCVSFIAFKPPGCQESGKAHHHHHH*"
     misc_feature    5607..5624
                     /label="6xHis"
                     /ApEinfo_revcolor="#bc8dbf"
                     /ApEinfo_fwdcolor="#bc8dbf"
     terminator      5704..5751
                     /label="T7 terminator (pET)"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     promoter        5977..6171
                     /label="bla promoter"
                     /ApEinfo_revcolor="#c4df9b"
                     /ApEinfo_fwdcolor="#c4df9b"
     CDS             6370..7029
                     /label="AmpR"
                     /ApEinfo_revcolor="#7bcdc8"
                     /ApEinfo_fwdcolor="#7bcdc8"
     origin          7187..284
                     /label="pBR322"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
ORIGIN
        1 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
       61 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
      121 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
      181 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
      241 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
      301 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
      361 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
      421 gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc
      481 atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc
      541 cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc
      601 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
      661 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
      721 ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag
      781 atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg
      841 cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc
      901 gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc
      961 acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa
     1021 ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc
     1081 gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg
     1141 aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg
     1201 aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt
     1261 cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg
     1321 gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag
     1381 atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg
     1441 gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat
     1501 ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc
     1561 gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt
     1621 tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag
     1681 ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct
     1741 gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca
     1801 taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt
     1861 cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag
     1921 tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca
     1981 tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct
     2041 gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc
     2101 gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgagatcccg
     2161 gtgcctaatg agtgagctaa cttacattaa ttgcgttgcg ctcactgccc gctttccagt
     2221 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     2281 tgcgtattgg gcgccagggt ggtttttctt ttcaccagtg agacgggcaa cagctgattg
     2341 cccttcaccg cctggccctg agagagttgc agcaagcggt ccacgctggt ttgccccagc
     2401 aggcgaaaat cctgtttgat ggtggttaac ggcgggatat aacatgagct gtcttcggta
     2461 tcgtcgtatc ccactaccga gatatccgca ccaacgcgca gcccggactc ggtaatggcg
     2521 cgcattgcgc ccagcgccat ctgatcgttg gcaaccagca tcgcagtggg aacgatgccc
     2581 tcattcagca tttgcatggt ttgttgaaaa ccggacatgg cactccagtc gccttcccgt
     2641 tccgctatcg gctgaatttg attgcgagtg agatatttat gccagccagc cagacgcaga
     2701 cgcgccgaga cagaacttaa tgggcccgct aacagcgcga tttgctggtg acccaatgcg
     2761 accagatgct ccacgcccag tcgcgtaccg tcttcatggg agaaaataat actgttgatg
     2821 ggtgtctggt cagagacatc aagaaataac gccggaacat tagtgcaggc agcttccaca
     2881 gcaatggcat cctggtcatc cagcggatag ttaatgatca gcccactgac gcgttgcgcg
     2941 agaagattgt gcaccgccgc tttacaggct tcgacgccgc ttcgttctac catcgacacc
     3001 accacgctgg cacccagttg atcggcgcga gatttaatcg ccgcgacaat ttgcgacggc
     3061 gcgtgcaggg ccagactgga ggtggcaacg ccaatcagca acgactgttt gcccgccagt
     3121 tgttgtgcca cgcggttggg aatgtaattc agctccgcca tcgccgcttc cactttttcc
     3181 cgcgttttcg cagaaacgtg gctggcctgg ttcaccacgc gggaaacggt ctgataagag
     3241 acaccggcat actctgcgac atcgtataac gttactggtt tcacattcac caccctgaat
     3301 tgactctctt ccgggcgcta tcatgccata ccgcgaaagg ttttgcgcca ttcgatggtg
     3361 tcgcgcaacg caattaatgt aagttagctc actcattagg cacaattctc atgtttgaca
     3421 gcttatcatc gactgcacgg tgcaccaatg cttctggcgt caggcagcca tcggaagctg
     3481 tggtatggct gtgcaggtcg taaatcactg cataattcgt gtcgctcaag gcgcactccc
     3541 gttctggata atgttttttg cgccgacatc ataacggttc tggcaaatat tctgaaatga
     3601 gctgttgaca attaatcatc ggctcgtata atgtgtggaa ttgtgagcgg ataacaattt
     3661 cacacaggaa acagccagtc cgtttaggtg ttttcacgag cacttcacca acaatAGGTG
     3721 CAGTaaggag atataccatg ccacaaacag agactAAAGC GGGTGTTGGG TTCCAAGCTG
     3781 GTGTTAAAGA TTATAAATTG ACTTACTATA CTCCTGAATA TGAAACCAAG GATACTGATA
     3841 TCTTGGCAGC ATTCCGAGTA TCGCCTCAAC CTGGAGTTCC ACCTGAAGAA GCAGGGGCTG
     3901 CGGTAGCTGC TGAATCTTCT ACTGGTACAT GGACAACTGT GTGGACCGAT GGGCTTACCA
     3961 GCCTTGATCG TTACAAAGGA CGATGCTACC ACATCGAGCC CGTTGCGGGG GAGGATAGTC
     4021 AATGGATCTG TTATGTAGCT TATCCCTTAG ACCTTTTTGA AGAAGGTTCG GTTACTAACA
     4081 TGTTTACCTC GATTGTGGGT AATGTATTTG GGTTCAAAGC CCTGCGCGCT CTACGTCTAG
     4141 AGGATCTGCG AATCCCTCCT ACCTACAGTA AAACTTTCCA AGGACCACCT CATGGTATCC
     4201 AAGTTGAAAG AGATAAATTG AACAAGTATG GACGTCCCCT ATTAGGATGT ACTATTAAAC
     4261 CAAAATTGGG GTTATCCGCG AAAAACTATG GTAGAGCATG TTATGAATGT CTACGTGGTG
     4321 GACTTGATTT TACCAAAGAT GATGAGAATG TGAACTCCCA ACCATTTATG CGTTGGAGaG
     4381 AtCGTTTCGT CTTTTGTGCC GAAGCTATTT ATAAATCACA GGCTGAAACA GGTGAAATCA
     4441 AAGGGCATTA TTTGAATGCT ACTGCGGGTA CATGCGAAGA AATGATCAAA AGAGCTGTAT
     4501 TTGCCAGAGA ATTGGGAGTT CCTATCGTAA TGCATGACTA CTTAACAGGG GGATTCACCG
     4561 CAAATACTAC GTTGGCGCAT TATTGCCGAG ATAATGGCCT ACTTCTTCAC ATCCACCGTG
     4621 CAATGCACGC TGTTATTGAT AGACAGAAGA ATCATGGTAT GCACTTCCGT GTACTAGCTA
     4681 AAGCTTTACG TATGTCTGGT GGAGATCATA TTCACTCGGG TACAGTAGTA GGTAAACTTG
     4741 AAGGAGAACG CGAAATGACT TTGGGCTTTG TTGATTTACT GCGCGATGAT TTTATTGAGA
     4801 AAGATCGTGC GCGCGGTATC TTTTTCACTC AAGATTGGGT tTCAATGCCG GGCGTCATAC
     4861 CTGTGGCTTC AGGGGGTATT CACGTTTGGC AcATGCCTGC TTTGACCGAG ATCTTTGGAG
     4921 ATGATTCTGT ACTACAATTC GGTGGAGGAA CTTTAGGCCA CCCTTGGGGA AATGCACCGG
     4981 GTGCCGCGGC CAACCGAGTA GCTCTGGAAG CATGTGTACA AGCTCGTAAT GAGGGACGTG
     5041 ATCTTGCACG TGAGGGTAAT GAAATTATCC GTGCGGCTTG CAAATGGAGT CCTGAACTAG
     5101 CTGCTGCTTG TGAAGTATGG AAAGCAATTA AATTCGAATT TGAGCCGGTC GATACGATTG
     5161 ACAAAAAGGT GTAAgctAGA AATAATTTTG Tttaacttta agaaggagat atacatatgT
     5221 GTATGCAAGT TTGGCCCATT GAAGGGATTA AAAAATTTGA AACCTTATCA TACCTCCCGC
     5281 CTCTTTCCAC TGAAGCCCTG CTGAAACAGG TGGATTATCT GATCCGTAGC AAATGGGTGC
     5341 CGTGCCTGGA GTTTTCGAAA GTGGGCTTTA TCTTCCGTGA ACATAATGCA AGCCCGGGAT
     5401 ATTACGATGG TCGGTACTGG ACGATGTGGA AGCTGCCAAT GTTTGGTTGC ACCGATGCCA
     5461 CACAGGTGTT GAACGAAGTT GAAGAGGTAA AAAAAGAATA TCCCGACGCG TATGTCCGCA
     5521 TAATTGGCTT CGACAACATG CGCCAGGTTC AATGTGTCTC TTTCATCGCG TTTAAACCGC
     5581 CGGGCTGTCA AGAGAGTGGT AAGGCTcatc accaccatca tcatTAAATC CGGCTGCTaa
     5641 caaagcccga aaggaagctg agtggctgct gccaccgctg agcaataaac cgctgagcaa
     5701 taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga
     5761 ggaactatat ccggatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc
     5821 ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac
     5881 acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga
     5941 tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt
     6001 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     6061 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     6121 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     6181 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     6241 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     6301 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     6361 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac
     6421 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     6481 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     6541 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     6601 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     6661 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca
     6721 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     6781 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     6841 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     6901 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     6961 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     7021 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     7081 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     7141 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     7201 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     7261 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     7321 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     7381 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     7441 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     7501 aaggcgcagc ggtcgggctg aa
//