LOCUS       Exported                1023 bp ds-DNA     linear   UNA 14-MAR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   yukikito
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Mar 21, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     primer_bind     1..35
                     /label=YK25
     misc_feature    19..919
                     /label=hGMCL1
     misc_feature    163..183
                     /label=NLS
     misc_feature    310..642
                     /label=BTB from Pfam
     primer_bind     498..517
                     /label=YK4
     misc_feature    655..918
                     /label=Bsch1 from Pfam
     CDS             934..957
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             973..996
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(989..1007)
                     /label=Primer 1_Rev
     primer_bind     complement(994..1023)
                     /label=YK40
ORIGIN
        1 cttggtaccg tgtgctggat gggatcgttg agcagccggg tgctgcgcca gccaagacca
       61 gcccttgccc agcaggcgca gggtgccagg gcggggggct cggcccggag gccggacact
      121 ggagacgatg cggcgggcca cggattctgt tactgtgcgg gcagccacaa gcgcaagcgg
      181 agcagcgggt ccttctgcta ctgtcaccct gactcggaga cggacgagga tgaggaggag
      241 ggggacgagc agcagcggct cctcaacacc cctcgaagga aaaaattaaa gagtacatct
      301 aaatatattt atcaaacatt atttttgaat ggtgaaaaca gtgacattaa gatttgtgct
      361 ctaggagaag aatggagctt acacaaaata tatttatgtc aatctggcta cttttctagt
      421 atgttcagtg gttcttggaa agaatccagc atgaatatta ttgaactgga gattcctgac
      481 cagaacattg atgtagaagc actgcaggtt gcatttggtt cactgtatcg agatgatgtc
      541 ttgataaagc ccagtcgagt tgttgccatt ttggcagcag cttgtttgct gcagttggac
      601 ggtttaatac agcagtgtgg tgagacaatg aaggaaacag ttaatgtgaa aactgtatgt
      661 ggctattaca catcagcagg gacctatgga ttagattctg taaagaaaaa gtgccttgaa
      721 tggcttctaa acaatttgat gactcaccag aatgttgaac tttttaaaga actcagtata
      781 aatgtcatga aacagctcat tggttcatct aacttatttg tgatgcaagt ggagatggat
      841 atatacactg ctctaaaaaa gtggatgttc cttcaacttg tgccttcttg gaatggatct
      901 ttaaaacagc ttttgacagg aggatcagga atggactaca aggacgacga tgacaaggta
      961 ccaggatcta tggattataa agatgatgat gataaagggt cggccgccag ctagggatcc
     1021 act
//
