LOCUS       pTCB-magroLC            5549 bp    DNA     circular SYN 22-OCT-2025
DEFINITION  none.
ACCESSION   test
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5549)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 22, 2025 from SnapGene 8.0.1
            https://www.snapgene.com
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     source          join(1..63,400..5549)
                     /mol_type="other DNA"
                     /label=source:synthetic DNA construct
                     /note="/ApEinfo_fwdcolor=#00DF87"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0 
                     -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
                     /note="/ApEinfo_hidden"
                     /note="/ApEinfo_label=source:synthetic DNA construct"
                     /note="/ApEinfo_revcolor=#00DF87"
                     /note="/locus_tag=source:synthetic DNA construct"
                     /organism="synthetic DNA construct"
     source          1..6
                     /mol_type="other DNA"
                     /label=source:synthetic DNA construct(1)
                     /note="/ApEinfo_fwdcolor=#00DF87"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0
                     -1 0 -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
                     /note="/ApEinfo_hidden"
                     /note="/ApEinfo_label=source:synthetic DNA construct"
                     /note="/ApEinfo_revcolor=#00DF87"
                     /note="/locus_tag=source:synthetic DNA construct(1)"
                     /note="/ApEinfo_fwdcolor=#00D95A"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0 
                     -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
                     /note="/ApEinfo_hidden"
                     /note="/ApEinfo_label=source:synthetic DNA construct(1)"
                     /note="/ApEinfo_revcolor=#00D95A"
                     /note="/locus_tag=source:synthetic DNA construct(1)"
                     /organism="synthetic DNA construct"
     source          724..1562
                     /mol_type="other DNA"
                     /label=source:synthetic DNA construct(2)
                     /note="/ApEinfo_fwdcolor=#00DF87"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0
                     -1 0 -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
                     /note="/ApEinfo_hidden"
                     /note="/ApEinfo_label=source:synthetic DNA construct"
                     /note="/ApEinfo_revcolor=#00DF87"
                     /note="/locus_tag=source:synthetic DNA construct(2)"
                     /note="/ApEinfo_fwdcolor=#008065"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0 
                     -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
                     /note="/ApEinfo_hidden"
                     /note="/ApEinfo_label=source:synthetic DNA construct(2)"
                     /note="/ApEinfo_revcolor=#008065"
                     /note="/locus_tag=source:synthetic DNA construct(2)"
                     /organism="synthetic DNA construct"
     source          1563..5549
                     /mol_type="other DNA"
                     /label=source:synthetic DNA construct(3)
                     /note="/ApEinfo_fwdcolor=#00DF87"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0
                     -1 0 -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
                     /note="/ApEinfo_hidden"
                     /note="/ApEinfo_label=source:synthetic DNA construct"
                     /note="/ApEinfo_revcolor=#00DF87"
                     /note="/locus_tag=source:synthetic DNA construct"
                     /note="/ApEinfo_fwdcolor=#13AAFE"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0 
                     -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
                     /note="/ApEinfo_hidden"
                     /note="/ApEinfo_label=source:synthetic DNA construct"
                     /note="/ApEinfo_revcolor=#13AAFE"
                     /note="/locus_tag=source:synthetic DNA construct(3)"
                     /organism="synthetic DNA construct"
     misc_feature    1..6
                     /locus_tag="XhoI"
                     /label=XhoI
                     /note="/ApEinfo_fwdcolor=cyan"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0
                     -1 0 -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
                     /note="/ApEinfo_label=XhoI"
                     /note="/ApEinfo_revcolor=cyan"
                     /note="/ApEinfo_fwdcolor=#7eff74"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0 
                     -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
                     /note="/ApEinfo_label=XhoI"
                     /note="/ApEinfo_revcolor=#7eff74"
     CDS             7..63
                     /codon_start=1
                     /locus_tag="leader sequence"
                     /label=leader sequence
                     /note="/ApEinfo_fwdcolor=#e9d024"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0 
                     -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
                     /note="/ApEinfo_label=leader sequence"
                     /note="/ApEinfo_revcolor=#e9d024"
                     /translation="MGWSCIILFLVATATGVHS"
     misc_feature    64..399
                     /label=magrolimab VL
     CDS             400..723
                     /codon_start=1
                     /locus_tag="Ab CL"
                     /label=Ab CL
                     /note="/ApEinfo_fwdcolor=#e9d024"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0 
                     -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
                     /note="/ApEinfo_label=Ab CL"
                     /note="/ApEinfo_revcolor=#e9d024"
                     /translation="RTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDN
                     ALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNR
                     GEC"
     misc_feature    724..729
                     /locus_tag="NheI"
                     /label=NheI
                     /note="/ApEinfo_fwdcolor=cyan"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0
                     -1 0 -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
                     /note="/ApEinfo_label=NheI"
                     /note="/ApEinfo_revcolor=cyan"
                     /note="/ApEinfo_fwdcolor=#7eff74"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0 
                     -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
                     /note="/ApEinfo_label=NheI"
                     /note="/ApEinfo_revcolor=#7eff74"
     polyA_signal    773..894
                     /locus_tag="SV40 poly(A) signal"
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="/ApEinfo_fwdcolor=#ff3eee"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0
                     -1 0 -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
                     /note="/ApEinfo_label=SV40 poly(A) signal"
                     /note="/ApEinfo_revcolor=#ff3eee"
                     /note="/ApEinfo_fwdcolor=#ff3eee"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0 
                     -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
                     /note="/ApEinfo_label=SV40 poly(A) signal"
                     /note="/ApEinfo_revcolor=#ff3eee"
     terminator      1009..1055
                     /locus_tag="T7 terminator(1)"
                     /label=T7 terminator(1)
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
                     /note="/ApEinfo_fwdcolor=#9d1b1c"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0
                     -1 0 -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
                     /note="/ApEinfo_label=T7 terminator"
                     /note="/ApEinfo_revcolor=#9d1b1c"
                     /note="/ApEinfo_fwdcolor=#9d1b1c"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0 
                     -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
                     /note="/ApEinfo_label=T7 terminator(1)"
                     /note="/ApEinfo_revcolor=#9d1b1c"
     terminator      1085..1221
                     /locus_tag="rrnG terminator(1)"
                     /label=rrnG terminator(1)
                     /note="transcription terminator from the E. coli ribosomal
                     RNA rrnG operon (Albrechtsen et al., 1991)"
                     /note="/ApEinfo_fwdcolor=#9d1b1c"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0
                     -1 0 -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
                     /note="/ApEinfo_label=rrnG terminator"
                     /note="/ApEinfo_revcolor=#9d1b1c"
                     /note="/ApEinfo_fwdcolor=#9d1b1c"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0 
                     -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
                     /note="/ApEinfo_label=rrnG terminator(1)"
                     /note="/ApEinfo_revcolor=#9d1b1c"
     misc_feature    1266..1337
                     /locus_tag="3' beta-globin insulator"
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
                     /note="/ApEinfo_fwdcolor=#7eff74"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0
                     -1 0 -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
                     /note="/ApEinfo_label=3' beta-globin insulator"
                     /note="/ApEinfo_revcolor=#7eff74"
                     /note="/ApEinfo_fwdcolor=#7eff74"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0 
                     -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
                     /note="/ApEinfo_label=3' beta-globin insulator"
                     /note="/ApEinfo_revcolor=#7eff74"
     rep_origin      complement(1563..2151)
                     /direction=LEFT
                     /locus_tag="ori"
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="/ApEinfo_fwdcolor=#999999"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0
                     -1 0 -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
                     /note="/ApEinfo_label=ori"
                     /note="/ApEinfo_revcolor=#999999"
                     /note="/ApEinfo_fwdcolor=#999999"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0 
                     -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
                     /note="/ApEinfo_label=ori"
                     /note="/ApEinfo_revcolor=#999999"
     CDS             2897..3757
                     /codon_start=1
                     /gene="bla"
                     /locus_tag="AmpR"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /note="/ApEinfo_fwdcolor=#e9d024"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0
                     -1 0 -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
                     /note="/ApEinfo_label=AmpR"
                     /note="/ApEinfo_revcolor=#e9d024"
                     /note="/ApEinfo_fwdcolor=#e9d024"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0 
                     -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
                     /note="/ApEinfo_label=AmpR"
                     /note="/ApEinfo_revcolor=#e9d024"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      3770..3906
                     /locus_tag="rrnG terminator"
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal
                     RNA rrnG operon (Albrechtsen et al., 1991)"
                     /note="/ApEinfo_fwdcolor=#9d1b1c"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0
                     -1 0 -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
                     /note="/ApEinfo_label=rrnG terminator"
                     /note="/ApEinfo_revcolor=#9d1b1c"
                     /note="/ApEinfo_fwdcolor=#9d1b1c"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0 
                     -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
                     /note="/ApEinfo_label=rrnG terminator"
                     /note="/ApEinfo_revcolor=#9d1b1c"
     misc_feature    4058..4129
                     /locus_tag="5' beta-globin insulator"
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
                     /note="/ApEinfo_fwdcolor=#7eff74"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0
                     -1 0 -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
                     /note="/ApEinfo_label=5' beta-globin insulator"
                     /note="/ApEinfo_revcolor=#7eff74"
                     /note="/ApEinfo_fwdcolor=#7eff74"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0 
                     -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
                     /note="/ApEinfo_label=5' beta-globin insulator"
                     /note="/ApEinfo_revcolor=#7eff74"
     terminator      4134..4180
                     /locus_tag="T7 terminator"
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
                     /note="/ApEinfo_fwdcolor=#9d1b1c"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0
                     -1 0 -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
                     /note="/ApEinfo_label=T7 terminator"
                     /note="/ApEinfo_revcolor=#9d1b1c"
                     /note="/ApEinfo_fwdcolor=#9d1b1c"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0 
                     -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
                     /note="/ApEinfo_label=T7 terminator"
                     /note="/ApEinfo_revcolor=#9d1b1c"
     polyA_signal    4198..4246
                     /locus_tag="synthetic polyadenylation signal"
                     /label=synthetic polyadenylation signal
                     /note="synthetic polyadenylation signal"
                     /note="/ApEinfo_fwdcolor=#ff3eee"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0
                     -1 0 -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
                     /note="/ApEinfo_label=synthetic polyadenylation signal"
                     /note="/ApEinfo_revcolor=#ff3eee"
                     /note="/ApEinfo_fwdcolor=#ff3eee"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0 
                     -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
                     /note="/ApEinfo_label=synthetic polyadenylation signal"
                     /note="/ApEinfo_revcolor=#ff3eee"
     enhancer        4327..4630
                     /locus_tag="CMV enhancer"
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="/ApEinfo_fwdcolor=#5ac3fa"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0
                     -1 0 -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
                     /note="/ApEinfo_label=CMV enhancer"
                     /note="/ApEinfo_revcolor=#5ac3fa"
                     /note="/ApEinfo_fwdcolor=#5ac3fa"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0 
                     -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
                     /note="/ApEinfo_label=CMV enhancer"
                     /note="/ApEinfo_revcolor=#5ac3fa"
     promoter        4632..4834
                     /locus_tag="CMV promoter"
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /note="/ApEinfo_fwdcolor=#346ee0"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0
                     -1 0 -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
                     /note="/ApEinfo_label=CMV promoter"
                     /note="/ApEinfo_revcolor=#346ee0"
                     /note="/ApEinfo_fwdcolor=#346ee0"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0 
                     -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
                     /note="/ApEinfo_label=CMV promoter"
                     /note="/ApEinfo_revcolor=#346ee0"
     intron          4968..5443
                     /locus_tag="beta-globin intron"
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
                     /note="/ApEinfo_fwdcolor=#eb606c"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0
                     -1 0 -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
                     /note="/ApEinfo_label=beta-globin intron"
                     /note="/ApEinfo_revcolor=#eb606c"
                     /note="/ApEinfo_fwdcolor=#eb606c"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0 
                     -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
                     /note="/ApEinfo_label=beta-globin intron"
                     /note="/ApEinfo_revcolor=#eb606c"
ORIGIN
        1 ctcgagatgg gatggtcatg tatcatcctt tttctagtag caactgcaac cggtgtacat
       61 tccgatgtgt tgatgacgca aaccccattg tctctcccag taagcctggg cgaccaagcg
      121 agtatctctt gccgctcatc ccaatcaatc gtttattcca atggcaacac atatctggga
      181 tggtacctcc aaaaaccagg gcaaagccca aagctgctta tatataaagt gtcaaatcga
      241 tttagcggag tcccggacag attttcaggg tcagggtccg gaacagattt tacgttgaaa
      301 atctctcgcg tagaagcaga ggacctgggg gtctatcatt gctttcaggg tagtcatgtc
      361 ccatatacct ttggcggtgg tacaaaggta gaaatcaagc gcaccgtcgc cgcgcccagc
      421 gtgttcatct tcccgcccag cgacgagcag ctgaagagcg gcacggccag cgtggtctgc
      481 ctgctcaaca acttctaccc ccgggaggcc aaggtgcagt ggaaggtgga caacgccctg
      541 cagtcgggga acagccagga gagcgtcacc gagcaggaca gcaaggacag cacctacagc
      601 ctgtcgagca ccctcacgct gagcaaggcc gactacgaga agcacaaggt gtacgcgtgc
      661 gaggtgaccc accagggcct gagcagcccc gtcaccaagt cgttcaaccg cggagaatgc
      721 tgagctagct tgactgactg agatacagcg taccttcagc tcacagacat gataagatac
      781 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
      841 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac
      901 aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc
      961 aagtaaaacc tctacaaatg tggtattggc ccatctctat cggtatcgta gcataacccc
     1021 ttggggcctc taaacgggtc ttgaggggtt ttttgtgccc ctcgggccgg attgctatct
     1081 accggcattg gcgcagaaaa aaatgcctga tgcgacgctg cgcgtcttat actcccacat
     1141 atgccagatt cagcaacgga tacggcttcc ccaacttgcc cacttccata cgtgtcctcc
     1201 ttaccagaaa tttatcctta aggtcgtcag ctatcctgca ggcgatctct cgatttcgat
     1261 caagacattc ctttaatggt cttttctgga caccactagg ggtcagaagt agttcatcaa
     1321 actttcttcc ctccctaatc tcattggtta ccttgggcta tcgaaactta attaaccagt
     1381 caagtcagct acttggcgag atcgacttgt ctgggtttcg actacgctca gaattgcgtc
     1441 agtcaagttc gatctggtcc ttgctattgc acccgttctc cgattacgag tttcatttaa
     1501 atcatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     1561 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     1621 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     1681 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     1741 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     1801 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     1861 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     1921 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     1981 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     2041 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     2101 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     2161 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     2221 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     2281 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     2341 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcatt taaatttccg
     2401 aactctccaa ggccctcgtc ggaaaatctt caaacctttc gtccgatcca tcttgcaggc
     2461 tacctctcga acgaactatc gcaagtctct tggccggcct tgcgccttgg ctattgcttg
     2521 gcagcgccta tcgccaggta ttactccaat cccgaatatc cgagatcggg atcacccgag
     2581 agaagttcaa cctacatcct caatcccgat ctatccgaga tccgaggaat atcgaaatcg
     2641 gggcgcgcct ggtgtaccga gaacgatcct ctcagtgcga gtctcgacga tccatatcgt
     2701 tgcttggcag tcagccagtc ggaatccagc ttgggaccca ggaagtccaa tcgtcagata
     2761 ttgtactcaa gcctggtcac ggcagcgtac cgatctgttt aaacctagat attgatagtc
     2821 tgatcggtca acgtataatc gagtcctagc ttttgcaaac atctatcaag agacaggatc
     2881 agcaggaggc tttcgcatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     2941 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     3001 agatcagttg ggtgcgcgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     3061 tgagagtttt cgccccgaag aacgctttcc aatgatgagc acttttaaag ttctgctatg
     3121 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     3181 ttctcagaat gacttggttg agtattcacc agtcacagaa aagcatctta cggatggcat
     3241 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     3301 acttctgaca acgattggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     3361 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     3421 gcgtgacacc acgatgcctg tagcaatggc aacaaccttg cgtaaactat taactggcga
     3481 actacttact ctagcttccc ggcaacagtt gatagactgg atggaggcgg ataaagttgc
     3541 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     3601 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     3661 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     3721 cgctgagata ggtgcctcac tgattaagca ttggtaaccg attctaggtg cattggcgca
     3781 gaaaaaaatg cctgatgcga cgctgcgcgt cttatactcc cacatatgcc agattcagca
     3841 acggatacgg cttccccaac ttgcccactt ccatacgtgt cctccttacc agaaatttat
     3901 ccttaagatc ccgaatcgtt taaactcgac tctggctcta tcgaatctcc gtcgtttcga
     3961 gcttacgcga acagccgtgg cgctcatttg ctcgtcgggc atcgaatctc gtcagctatc
     4021 gtcagcttac ctttttggca gcgatcgcgg ctcccgacat cttggaccat tagctccaca
     4081 ggtatcttct tccctctagt ggtcataaca gcagcttcag ctacctctca attcaaaaaa
     4141 cccctcaaga cccgtttaga ggccccaagg ggttatgcta tcaatcgttg cgttacacac
     4201 acaaaaaacc aacacacatc catcttcgat ggatagcgat tttattatct aactgctgat
     4261 cgagtgtagc cagatctagt aatcaattac ggggtcatta gttcatagcc catatatgga
     4321 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     4381 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     4441 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     4501 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     4561 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     4621 tattaccatg ctgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
     4681 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
     4741 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
     4801 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatcgcctg
     4861 gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccc
     4921 ctcgaagctt acatgtggta ccgagctcgg atcctgagaa cttcagggtg agtctatggg
     4981 acccttgatg ttttctttcc ccttcttttc tatggttaag ttcatgtcat aggaagggga
     5041 gaagtaacag ggtacacata ttgaccaaat cagggtaatt ttgcatttgt aattttaaaa
     5101 aatgctttct tcttttaata tacttttttg tttatcttat ttctaatact ttccctaatc
     5161 tctttctttc agggcaataa tgatacaatg tatcatgcct ctttgcacca ttctaaagaa
     5221 taacagtgat aatttctggg ttaaggcaat agcaatattt ctgcatataa atatttctgc
     5281 atataaattg taactgatgt aagaggtttc atattgctaa tagcagctac aatccagcta
     5341 ccattctgct tttattttat ggttgggata aggctggatt attctgagtc caagctaggc
     5401 ccttttgcta atcatgttca tacctcttat cttcctccca cagctcctgg gcaacgtgct
     5461 ggtctgtgtg ctggcccatc actttggcaa aggcggccgc tgccaagctt ccgagctctc
     5521 gaattcaaag gaggtaccgc gatatctac
//
