LOCUS       pTCB-avelLC             5537 bp    DNA     circular SYN 03-APR-2025
DEFINITION  none.
ACCESSION   test
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5537)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 3, 2025 from SnapGene 8.0.1
            https://www.snapgene.com
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     source          1..5537
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          1..6
                     /mol_type="other DNA"
                     /label=source:synthetic DNA construct(1)
                     /note="/ApEinfo_fwdcolor=#00DF87"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0
                     -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
                     /note="/ApEinfo_hidden"
                     /note="/ApEinfo_label=source:synthetic DNA construct"
                     /note="/ApEinfo_revcolor=#00DF87"
                     /note="/locus_tag=source:synthetic DNA construct(1)"
                     /organism="synthetic DNA construct"
     source          712..1550
                     /mol_type="other DNA"
                     /label=source:synthetic DNA construct(2)
                     /note="/ApEinfo_fwdcolor=#00DF87"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0
                     -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
                     /note="/ApEinfo_hidden"
                     /note="/ApEinfo_label=source:synthetic DNA construct"
                     /note="/ApEinfo_revcolor=#00DF87"
                     /note="/locus_tag=source:synthetic DNA construct(2)"
                     /organism="synthetic DNA construct"
     source          1551..5537
                     /mol_type="other DNA"
                     /label=source:synthetic DNA construct
                     /note="/ApEinfo_fwdcolor=#00DF87"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0
                     -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
                     /note="/ApEinfo_hidden"
                     /note="/ApEinfo_label=source:synthetic DNA construct"
                     /note="/ApEinfo_revcolor=#00DF87"
                     /note="/locus_tag=source:synthetic DNA construct"
                     /organism="synthetic DNA construct"
     misc_feature    1..6
                     /locus_tag="XhoI"
                     /label=XhoI
                     /note="/ApEinfo_fwdcolor=cyan"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0
                     -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
                     /note="/ApEinfo_label=XhoI"
                     /note="/ApEinfo_revcolor=cyan"
     CDS             7..63
                     /codon_start=1
                     /label=leader sequence
                     /translation="MGWSCIILFLVATATGVHS"
     misc_feature    64..708
                     /label=avelumab LC
     misc_feature    712..717
                     /locus_tag="NheI"
                     /label=NheI
                     /note="/ApEinfo_fwdcolor=cyan"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0
                     -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
                     /note="/ApEinfo_label=NheI"
                     /note="/ApEinfo_revcolor=cyan"
     polyA_signal    761..882
                     /locus_tag="SV40 poly(A) signal"
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="/ApEinfo_fwdcolor=#ff3eee"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0
                     -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
                     /note="/ApEinfo_label=SV40 poly(A) signal"
                     /note="/ApEinfo_revcolor=#ff3eee"
     terminator      997..1043
                     /locus_tag="T7 terminator(1)"
                     /label=T7 terminator(1)
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
                     /note="/ApEinfo_fwdcolor=#9d1b1c"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0
                     -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
                     /note="/ApEinfo_label=T7 terminator"
                     /note="/ApEinfo_revcolor=#9d1b1c"
     terminator      1073..1209
                     /locus_tag="rrnG terminator(1)"
                     /label=rrnG terminator(1)
                     /note="transcription terminator from the E. coli ribosomal
                     RNA rrnG operon (Albrechtsen et al., 1991)"
                     /note="/ApEinfo_fwdcolor=#9d1b1c"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0
                     -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
                     /note="/ApEinfo_label=rrnG terminator"
                     /note="/ApEinfo_revcolor=#9d1b1c"
     misc_feature    1254..1325
                     /locus_tag="3' beta-globin insulator"
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
                     /note="/ApEinfo_fwdcolor=#7eff74"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0
                     -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
                     /note="/ApEinfo_label=3' beta-globin insulator"
                     /note="/ApEinfo_revcolor=#7eff74"
     rep_origin      complement(1551..2139)
                     /direction=LEFT
                     /locus_tag="ori"
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="/ApEinfo_fwdcolor=#999999"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0
                     -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
                     /note="/ApEinfo_label=ori"
                     /note="/ApEinfo_revcolor=#999999"
     CDS             2885..3745
                     /codon_start=1
                     /gene="bla"
                     /locus_tag="AmpR"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /note="/ApEinfo_fwdcolor=#e9d024"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0
                     -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
                     /note="/ApEinfo_label=AmpR"
                     /note="/ApEinfo_revcolor=#e9d024"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      3758..3894
                     /locus_tag="rrnG terminator"
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal
                     RNA rrnG operon (Albrechtsen et al., 1991)"
                     /note="/ApEinfo_fwdcolor=#9d1b1c"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0
                     -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
                     /note="/ApEinfo_label=rrnG terminator"
                     /note="/ApEinfo_revcolor=#9d1b1c"
     misc_feature    4046..4117
                     /locus_tag="5' beta-globin insulator"
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
                     /note="/ApEinfo_fwdcolor=#7eff74"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0
                     -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
                     /note="/ApEinfo_label=5' beta-globin insulator"
                     /note="/ApEinfo_revcolor=#7eff74"
     terminator      4122..4168
                     /locus_tag="T7 terminator"
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
                     /note="/ApEinfo_fwdcolor=#9d1b1c"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0
                     -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
                     /note="/ApEinfo_label=T7 terminator"
                     /note="/ApEinfo_revcolor=#9d1b1c"
     polyA_signal    4186..4234
                     /locus_tag="synthetic polyadenylation signal"
                     /label=synthetic polyadenylation signal
                     /note="synthetic polyadenylation signal"
                     /note="/ApEinfo_fwdcolor=#ff3eee"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0
                     -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
                     /note="/ApEinfo_label=synthetic polyadenylation signal"
                     /note="/ApEinfo_revcolor=#ff3eee"
     enhancer        4315..4618
                     /locus_tag="CMV enhancer"
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="/ApEinfo_fwdcolor=#5ac3fa"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0
                     -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
                     /note="/ApEinfo_label=CMV enhancer"
                     /note="/ApEinfo_revcolor=#5ac3fa"
     promoter        4620..4822
                     /locus_tag="CMV promoter"
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /note="/ApEinfo_fwdcolor=#346ee0"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0
                     -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
                     /note="/ApEinfo_label=CMV promoter"
                     /note="/ApEinfo_revcolor=#346ee0"
     intron          4956..5431
                     /locus_tag="beta-globin intron"
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
                     /note="/ApEinfo_fwdcolor=#eb606c"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 0.5 0 1 2 0 0
                     -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
                     /note="/ApEinfo_label=beta-globin intron"
                     /note="/ApEinfo_revcolor=#eb606c"
ORIGIN
        1 ctcgagatgg gatggtcatg tatcatcctt tttctagtag caactgcaac cggtgtacat
       61 tcccagagtg ccctgacgca gcctgcttct gtctctggga gtccagggca gtccattacc
      121 attagttgta ctggtacatc ctctgacgtg ggtgggtata actatgttag ttggtatcaa
      181 cagcatcccg gtaaagctcc caaactgatg atctacgacg tgagtaatag gcccagcggt
      241 gttagtaaca ggttttcagg atctaagagt ggtaacacag cgtcattgac tataagtggg
      301 ctgcaagctg aggatgaagc tgattactac tgctcatcat atacgtcatc aagtacaaga
      361 gtatttggga ccggtacgaa agtcacagtc cttggacagc caaaagccaa tccgacagta
      421 actttgttcc cgccaagctc agaggagctg caagcgaata aagcaactct tgtgtgtctg
      481 ataagtgatt tttacccggg agcggtcact gtggcgtgga aggcagacgg gtcaccagtt
      541 aaggctggag tggagaccac gaaaccgagc aagcaaagca acaacaaata tgccgcatca
      601 tcatatctgt ctctgacccc cgagcagtgg aagtcccatc gaagttactc ttgccaggtc
      661 acccatgagg gctctactgt tgagaagacg gtcgctccca cggagtgtta agctagcttg
      721 actgactgag atacagcgta ccttcagctc acagacatga taagatacat tgatgagttt
      781 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
      841 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt
      901 cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc
      961 tacaaatgtg gtattggccc atctctatcg gtatcgtagc ataacccctt ggggcctcta
     1021 aacgggtctt gaggggtttt ttgtgcccct cgggccggat tgctatctac cggcattggc
     1081 gcagaaaaaa atgcctgatg cgacgctgcg cgtcttatac tcccacatat gccagattca
     1141 gcaacggata cggcttcccc aacttgccca cttccatacg tgtcctcctt accagaaatt
     1201 tatccttaag gtcgtcagct atcctgcagg cgatctctcg atttcgatca agacattcct
     1261 ttaatggtct tttctggaca ccactagggg tcagaagtag ttcatcaaac tttcttccct
     1321 ccctaatctc attggttacc ttgggctatc gaaacttaat taaccagtca agtcagctac
     1381 ttggcgagat cgacttgtct gggtttcgac tacgctcaga attgcgtcag tcaagttcga
     1441 tctggtcctt gctattgcac ccgttctccg attacgagtt tcatttaaat catgtgagca
     1501 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     1561 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     1621 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     1681 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     1741 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     1801 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     1861 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     1921 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     1981 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     2041 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     2101 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     2161 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     2221 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     2281 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     2341 tcagcgatct gtctatttcg ttcatccata gttgcattta aatttccgaa ctctccaagg
     2401 ccctcgtcgg aaaatcttca aacctttcgt ccgatccatc ttgcaggcta cctctcgaac
     2461 gaactatcgc aagtctcttg gccggccttg cgccttggct attgcttggc agcgcctatc
     2521 gccaggtatt actccaatcc cgaatatccg agatcgggat cacccgagag aagttcaacc
     2581 tacatcctca atcccgatct atccgagatc cgaggaatat cgaaatcggg gcgcgcctgg
     2641 tgtaccgaga acgatcctct cagtgcgagt ctcgacgatc catatcgttg cttggcagtc
     2701 agccagtcgg aatccagctt gggacccagg aagtccaatc gtcagatatt gtactcaagc
     2761 ctggtcacgg cagcgtaccg atctgtttaa acctagatat tgatagtctg atcggtcaac
     2821 gtataatcga gtcctagctt ttgcaaacat ctatcaagag acaggatcag caggaggctt
     2881 tcgcatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     2941 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     3001 tgcgcgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     3061 ccccgaagaa cgctttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     3121 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     3181 cttggttgag tattcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     3241 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     3301 gattggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     3361 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     3421 gatgcctgta gcaatggcaa caaccttgcg taaactatta actggcgaac tacttactct
     3481 agcttcccgg caacagttga tagactggat ggaggcggat aaagttgcag gaccacttct
     3541 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     3601 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     3661 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     3721 tgcctcactg attaagcatt ggtaaccgat tctaggtgca ttggcgcaga aaaaaatgcc
     3781 tgatgcgacg ctgcgcgtct tatactccca catatgccag attcagcaac ggatacggct
     3841 tccccaactt gcccacttcc atacgtgtcc tccttaccag aaatttatcc ttaagatccc
     3901 gaatcgttta aactcgactc tggctctatc gaatctccgt cgtttcgagc ttacgcgaac
     3961 agccgtggcg ctcatttgct cgtcgggcat cgaatctcgt cagctatcgt cagcttacct
     4021 ttttggcagc gatcgcggct cccgacatct tggaccatta gctccacagg tatcttcttc
     4081 cctctagtgg tcataacagc agcttcagct acctctcaat tcaaaaaacc cctcaagacc
     4141 cgtttagagg ccccaagggg ttatgctatc aatcgttgcg ttacacacac aaaaaaccaa
     4201 cacacatcca tcttcgatgg atagcgattt tattatctaa ctgctgatcg agtgtagcca
     4261 gatctagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
     4321 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
     4381 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
     4441 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
     4501 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
     4561 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatgct
     4621 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
     4681 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
     4741 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
     4801 ggaggtctat ataagcagag ctggtttagt gaaccgtcag atcgcctgga gacgccatcc
     4861 acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctcccct cgaagcttac
     4921 atgtggtacc gagctcggat cctgagaact tcagggtgag tctatgggac ccttgatgtt
     4981 ttctttcccc ttcttttcta tggttaagtt catgtcatag gaaggggaga agtaacaggg
     5041 tacacatatt gaccaaatca gggtaatttt gcatttgtaa ttttaaaaaa tgctttcttc
     5101 ttttaatata cttttttgtt tatcttattt ctaatacttt ccctaatctc tttctttcag
     5161 ggcaataatg atacaatgta tcatgcctct ttgcaccatt ctaaagaata acagtgataa
     5221 tttctgggtt aaggcaatag caatatttct gcatataaat atttctgcat ataaattgta
     5281 actgatgtaa gaggtttcat attgctaata gcagctacaa tccagctacc attctgcttt
     5341 tattttatgg ttgggataag gctggattat tctgagtcca agctaggccc ttttgctaat
     5401 catgttcata cctcttatct tcctcccaca gctcctgggc aacgtgctgg tctgtgtgct
     5461 ggcccatcac tttggcaaag gcggccgctg ccaagcttcc gagctctcga attcaaagga
     5521 ggtaccgcga tatctac
//
