LOCUS       TG_GFP_nsec_aid_1       8521 bp    DNA     circular SYN 19-MAR-2025
DEFINITION  TG_GFP_nsec_aid_1.
ACCESSION   TG_GFP_nsec_aid_1
VERSION     TG_GFP_nsec_aid_1
KEYWORDS    .
SOURCE      
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     CDS             4502..5524
                     /note="pLannotate"
                     /label="hph"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        7386..7490
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             7491..8351
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.7"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(5891..6733)
                     /note="pLannotate"
                     /label="mEGFP (fragment)"
                     /database="snapgene"
                     /identity="97.8"
                     /match_length="97.6"
                     /fragment="True"
                     /other="CDS"
     rep_origin      1..589
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             1621..2700
                     /note="pLannotate"
                     /label="cre"
                     /database="swissprot"
                     /identity="95.3"
                     /match_length="105.0"
                     /fragment="False"
                     /other="CDS"
     protein_bind    complement(5641..5674)
                     /note="pLannotate"
                     /label="loxP"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     protein_bind    complement(1181..1214)
                     /note="pLannotate"
                     /label="loxP"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     promoter        913..943
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    877..898
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     protein_bind    951..967
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             6892..7049
                     /note="pLannotate"
                     /label="lacZα (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="90.8"
                     /fragment="True"
                     /other="CDS"
     CDS             3121..4092
                     /note="pLannotate"
                     /label="sqt-1"
                     /database="swissprot"
                     /identity="99.7"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     misc_feature    complement(7055..7154)
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="70.9"
                     /fragment="True"
                     /other="misc_feature"
     CDS             complement(5717..5893)
                     /note="pLannotate"
                     /label="IAA17 (fragment)"
                     /database="swissprot"
                     /identity="81.4"
                     /match_length="25.8"
                     /fragment="True"
                     /other="CDS"
     CDS             772..864
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.6"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(7256..7291)
                     /note="pLannotate"
                     /label="rop (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="18.8"
                     /fragment="True"
                     /other="CDS"
     terminator      2709..2743
                     /note="pLannotate"
                     /label="tbb-2 terminator (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="10.8"
                     /fragment="True"
                     /other="terminator"
     CDS             complement(1219..1272)
                     /note="pLannotate"
                     /label="hsp-16.1 (fragment)"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="12.4"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaac agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctcg ttatcgattt
     1021 cctaggttca caattttcct gctcggacat gtcatttgcc ctactagtcc ggaggctccg
     1081 gaggctcctg caccagaacc tccgccagaa ccgcctccgg atcccgatgc agatcctccg
     1141 ctagcctgaa aatttaaata gcagactctc gttcatgtgt ataacttcgt ataatgtatg
     1201 ctatacgaag ttattcgcga gatcaccaaa aacggaacgt tgagctggac ggaaatagtg
     1261 gtaaagtgac atgattatag tttgaagatt tctaatttca caattagagc aaatgttgtt
     1321 cggtatttat tttcaacggt atttatacta ttttccacct ttttctagaa cattcgagct
     1381 gcttgttgca aaaggagggc gactcacatt cggtacatgg aaaagtagtg tacacaataa
     1441 agagacccag atacattttc cgtctgcgtc tctttgcacc caccgggagt attttcaaac
     1501 gaatgcatct aggaccttct agaacattct gtaaggctgc agaatgcggg tatataagga
     1561 aagcgggctc agaggaagcc aacacgcttt gttctagtgc atctaaaaaa cttcgaaaat
     1621 atgtcaaatc ttctcactgt tcatcaaaat cttccagcac ttccagttga cgcgacaagt
     1681 gatgaagttc gcaagaacct catggatatg ttccgtgaca gacaagcctt ctcggaacac
     1741 acttggaaga tgcttctctc agtgtgccgt tcttgggctg cctggtgcaa gcttaacaac
     1801 cgcaagtggt tcccagccga gccagaagac gttcgtgact acttgctcta cctccaagct
     1861 cgtggattgg cggtgaagac gatccaacaa catctcggac agctcaatat gctccatcgc
     1921 cgatctggac ttccacgccc atcagattct aatgccgtat ctctcgtaat gagacgtatc
     1981 cgcaaggaaa atgttgatgc tggagagaga gccaagcaag cccttgcatt cgagaggtaa
     2041 gtttaaacat gattttacta actaactaat ctgatttaaa ttttcagaac cgacttcgat
     2101 caggtccgtt ctcttatgga aaattccgat cgttgccagg acatccgaaa cctcgccttc
     2161 ctcggaattg cttacaacac acttctccgt atcgccgaaa ttgcccgcat tcgcgtcaag
     2221 gatattagtc gcacagacgg aggaagaatg ctcatccaca tcggacgtac aaaaacgctg
     2281 gtctcgactg ccggagtcga aaaagcgctc tccctgggcg tgactaagct agttgagcgc
     2341 tggatttcgg tttccggagt cgccgatgat ccaaataatt atctattttg tcgtgttcgc
     2401 aagaacggag ttgctgcccc gagtgcaaca tcacaactct cgactcgagc tcttgaagga
     2461 atcttcgaag ctactcatcg cctgatctac ggagccaagg atgattccgg acagcggtat
     2521 ttagcgtggt ctggacattc cgcccgtgtc ggagctgccc gtgacatggc tcgcgctgga
     2581 gtttccatcc cagagattat gcaagccgga ggttggacca acgtcaacat tgtgatgaac
     2641 tatattagaa acttggattc tgaaactggc gccatggttc gtcttctcga agacggtgat
     2701 taagataaat gcaaaatcct ttcaagcatt cccttcttct ctacgtacgc tgccctcgat
     2761 tgtctatctt ccattctcac caagtttcaa caactttatt cagtcattct tctctggttc
     2821 atttcccaac caatcttccc gaataaagcc catctgtcct ttcggccaca ttcctcacct
     2881 acatccgtac tccttatctc ccgttcttat ccagtctttt atcgtctctg cgtctcgcac
     2941 acgtgtctcg tcggtaatgt atataaatat caaaaaaaac ttgaaaactt gcggaaagat
     3001 ttcctaataa aaggagacga atccggtagg aaatcgacaa gtctctgctt ctcgaagact
     3061 aggtgagtta catttctttg gccgaattat tttgaaatcc taaatttgat aatttcagag
     3121 atgtctgtaa aattggcttg ctacgtcacc gcttcggtca ctgtcgccac tcttatggta
     3181 tgcttcatga ccatgtcaac catctactcg gaggttgatg gattcagaga gaagcttgat
     3241 accgagatga atgtgttcag acaatctacc aatggattgt ggaaggacat agttgtcatc
     3301 ggaagatcta gcaagcgtgt ccgttgtcaa tatgaagaga ccaacgctac cccaactcca
     3361 catgctgatg gatccccatc tgcacctccg ggacagccac cagcagttcc accagtcttc
     3421 aaccagccaa agactccaaa tggagccaat ggaaatggac ctacgtgtaa ctgcaacgct
     3481 gataacaagt gtccagctgg accatctgga ccaaaaggag ttccaggagt tccaggactt
     3541 gatggagtcc caggattgga cggtgttcca ggagttggag ctgatgatat cgctccacaa
     3601 cgcgagtctg tcggatgctt cacttgccca caaggaccag ttggaccacc aggagctctt
     3661 ggaagaccag gaccacgtgg acttcctggc cccaggggtc aaaatggaaa cccaggaaga
     3721 gatggacaac caggacatcc aggagagcaa ggatcatccg gtcaaatcgg aaagatcgga
     3781 gagccaggac caccaggaga gaagggacgc gacgccgagc atccaatcgg aagaccagga
     3841 ccaaagggac caagaggaga tcaaggacca acaggaccag ctggacagaa cggtcttcac
     3901 ggaccaccag gagagccagg aaccgttgga ccagaaggac catctggaaa gcaaggacgt
     3961 caaggaccag acggaaccca gggagagact ggaccagacg gaagaccagg aaaggatgcc
     4021 gagtactgcc agtgcccaga caagtctcca ccatcagaag cagtcaacgc caaccgtgga
     4081 tacagaaata tctaaattgt tggtgttttc taataaaata tttgagattc aaggcctatt
     4141 tttgctttcg tcgtaaatct acacacgcgt ctcttccgtg cgagagtcca agccagcagc
     4201 caaattcgtt gagtgagtat tcaacgttta tacgttgtca ggcaacgaga aataggaaaa
     4261 tgcatcggga aatgttcttt tttcgatttt ttccaaggtt ttgacaaatt ttaccacgaa
     4321 ttttgctatg ttttcaatta aaaaatatgt tattcaactg tttctatgag gaaaataagg
     4381 ctttgcatgt aattttctta ttcagcataa tttttaatta atttgaattt tctgtcctaa
     4441 cgtttatttt gttttcttgg ttatgactga tgtgaaatta atttttgaat tttaaggtaa
     4501 tatgaaaaag cctgaactga ctgcgacgtc tgtcgagaag tttctgatcg aaaagttcga
     4561 cagcgtctcc gacctgatgc agctctcgga gggcgaagaa tctcgtgctt tcagcttcga
     4621 tgtaggaggg cgtggatatg tcctgcgcgt aaatagctgc gccgatggtt tctacaaaga
     4681 tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg attccggaag tgcttgacat
     4741 tggggaattc agcgagagcc tgacctattg catctcccgc cgtgcacagg gtgtcacgtt
     4801 gcaagacctg cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg aggccatgga
     4861 tgcgatcgct gcggccgatc ttagccagac gagcgggttc ggcccattcg gaccgcaagg
     4921 aatcggtcaa tacactacat ggcgtgattt catatgcgcg attgctgatc cccatgtgta
     4981 tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga
     5041 gctgatgctt tgggccgagg actgccccga agtccggcac ctcgtgcacg cggatttcgg
     5101 ctccaacaac gtactaacag acaatggccg cataacagcg gtcattgact ggagcgaggc
     5161 gatgttcggg gattcccaat acgaggtcgc caacatcttc ttctggaggc cgtggttggc
     5221 ttgtatggag cagcagacgc gctacttcga gcggaggcat ccggagcttg caggatcgcc
     5281 gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga gcttggttga
     5341 cggcaatttc gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg
     5401 agccgggact gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg
     5461 ctgtgtagaa gtactcgccg atagtggaaa caggaggcca tcgacgcggc cccgggccaa
     5521 ggagtagatt ttcaaatttt aaatactgaa tatttgtttt ttttcctatt atttatttat
     5581 tctctttgtg ttttttttct tgctttgtaa aaaattaatt caatccaaat ctaagcatgc
     5641 ataacttcgt ataatgtatg ctatacgaag ttatctcccg ttcgtgggag gcccagatgc
     5701 gcgcttaagt tatatatttg tttaaactta cctttccgga ttgaccggtc ttgacgaagg
     5761 cggcggcctc tggtcctccg gaggactttt ggcaggagac catgacgttc ttacggtagg
     5821 aacggactgg tggccatccg acgacttggg ccttggctgg tggcttggct gggtcctttg
     5881 gcatagatcc cttgtagagc tcgtccattc cgtgggtgat tccggcggcg gtgacgaact
     5941 cgaggaggac catgtggtca cgcttctcgt ttgggtcctt ggagagggcg gattgggtgg
     6001 agaggtagtg gttgtctggg aggaggactg gtccgtctcc gattggggtg ttttgttggt
     6061 agtggtcggc gagttggacg gatccgtcct cgatgttgtg acggatctga aaatttaaat
     6121 cagattagtt agttagtaaa atcatgttta aacttacctt gaagttgacc ttgattccgt
     6181 tcttttgctt gtcggccatg atgtagacgt tgtgggagtt gtagttgtac tcgagcttgt
     6241 gtccgaggat gtttccgtcc tccttgaagt cgattccctg aaaatttaaa tatgtatggt
     6301 tagttagtac cgaactgttt aaacttacct tgagctcgat acggttgacg agggtgtctc
     6361 cctcgaactt gacctcggca cgggtcttgt agtttccgtc gtcctgaaaa tttaaataat
     6421 cagtagttag tatatatatg tttaaactta ccttgaagaa gatggtacgc tcttggacgt
     6481 atccctctgg catggcggac ttgaagaagt cgtgacgctt catgtggtct gggtaacggg
     6541 agaagcattg gactccgtag cagaaggtgg tgacgagggt tggccatggg actgggagct
     6601 ttccggtggt gcagatgaac ttgagggtga gctttccgta ggtggcgtct ccctctccct
     6661 ctccggagac ggagaacttg tgtccgttga cgtctccgtc gagctcgacg aggattggga
     6721 caactccagt gaacaattct tctcctttac tcatcgatgc tccgtcgagg gaacggaatt
     6781 gagcaagttg ctcggaggaa acagatccag actccttcct aggctcgcag ttctcttgtt
     6841 cagacatatc gttagcacga ctagtgcatt gggatatccc aatacgcgtc aattcactgg
     6901 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     6961 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     7021 cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt ctccttacgc
     7081 atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg
     7141 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     7201 tgctcccggc atccgcttac agacaagctg tgacagtctc cgggagctgc atgtgtcaga
     7261 ggttttcacc gtcatcaccg aaacgcgcga gacaaaaggg cctcgtgata cgcctatttt
     7321 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     7381 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     7441 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     7501 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     7561 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     7621 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     7681 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     7741 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     7801 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     7861 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     7921 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     7981 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     8041 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     8101 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     8161 cggctggctg gtttattgct gataaatctg gagccggtga gcgtggttct cgcggtatca
     8221 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     8281 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     8341 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     8401 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     8461 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     8521 c
//
