LOCUS       gyrA462_mutation        2628 bp ds-DNA     linear       19-APR-2022
DEFINITION  .
KEYWORDS    "creator:Triple Threat"
FEATURES             Location/Qualifiers
     gene            1..2628
                     /label="gyrA"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="gene: gyrA gene_synonym: ECK2223; hisW; nalA; nfxA; norA; parD db_xref: ASAP: ABE-0007370 db_xref: ECOCYC: EG10423 db_xref: GeneID: 946614 locus_tag: b2231"
     CDS             1..2628
                     /label="gyrA"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="gene: gyrA codon_start: 1 gene_synonym: ECK2223; hisW; nalA; nfxA; norA; parD translation: MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPVHRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYMLVDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMPTKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFPTAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEKIAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINMVALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDPIIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYLTEQQAQAILDLCLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELELVREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKGKSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLPLEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDELIGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRGDGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMITDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAAEGDDEIAPEVDVDDEPEEE* product: DNA gyrase subunit A db_xref: UniProtKB/Swiss-Prot: P0AES4 db_xref: ASAP: ABE-0007370 db_xref: ECOCYC: EG10423 db_xref: GeneID: 946614 locus_tag: b2231 EC_number: 5.6.2.2 protein_id: NP_416734.1 transl_table: 11"
     source          1..2628
                     /label="source"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="organism: Escherichia coli str. K-12 substr. MG1655 strain: K-12 mol_type: genomic DNA: sub_strain: MG1655 db_xref: taxon: 511145"
     misc_feature    1377..1377
                     /label="silent mutation"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    1384..1384
                     /label="arg to cys mutation"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
ORIGIN
        1 atgagcgacc ttgcgagaga aattacaccg gtcaacattg aggaagagct gaagagctcc
       61 tatctggatt atgcgatgtc ggtcattgtt ggccgtgcgc tgccagatgt ccgagatggc
      121 ctgaagccgg tacaccgtcg cgtactttac gccatgaacg tactaggcaa tgactggaac
      181 aaagcctata aaaaatctgc ccgtgtcgtt ggtgacgtaa tcggtaaata ccatccccat
      241 ggtgactcgg cggtctatga cacgatcgtc cgcatggcgc agccattctc gctgcgttat
      301 atgctggtag acggtcaggg taacttcggt tctatcgacg gcgactctgc ggcggcaatg
      361 cgttatacgg aaatccgtct ggcgaaaatt gcccatgaac tgatggccga tctcgaaaaa
      421 gagacggtcg atttcgttga taactatgac ggcacggaaa aaattccgga cgtcatgcca
      481 accaaaattc ctaacctgct ggtgaacggt tcttccggta tcgccgtagg tatggcaacc
      541 aacatcccgc cgcacaacct gacggaagtc atcaacggtt gtctggcgta tattgatgat
      601 gaagacatca gcattgaagg gctgatggaa cacatcccgg ggccggactt cccgacggcg
      661 gcaatcatta acggtcgtcg cggtattgaa gaagcttacc gtaccggtcg cggcaaggtg
      721 tatatccgcg ctcgcgcaga agtggaagtt gacgccaaaa ccggtcgtga aaccattatc
      781 gtccacgaaa ttccgtatca ggtaaacaaa gcgcgcctga tcgagaagat tgcggaactg
      841 gtaaaagaaa aacgcgtgga aggcatcagc gcgctgcgtg acgagtctga caaagacggt
      901 atgcgcatcg tgattgaagt gaaacgcgat gcggtcggtg aagttgtgct caacaacctc
      961 tactcccaga cccagttgca ggtttctttc ggtatcaaca tggtggcatt gcaccatggt
     1021 cagccgaaga tcatgaacct gaaagacatc atcgcggcgt ttgttcgtca ccgccgtgaa
     1081 gtggtgaccc gtcgtactat tttcgaactg cgtaaagctc gcgatcgtgc tcatatcctt
     1141 gaagcattag ccgtggcgct ggcgaacatc gacccgatca tcgaactgat ccgtcatgcg
     1201 ccgacgcctg cagaagcgaa aactgcgctg gttgctaatc cgtggcagct gggcaacgtt
     1261 gccgcgatgc tcgaacgtgc tggcgacgat gctgcgcgtc cggaatggct ggagccagag
     1321 ttcggcgtgc gtgatggtct gtactacctg accgaacagc aagctcaggc gattcttgat
     1381 ctgtgtttgc agaaactgac cggtcttgag cacgaaaaac tgctcgacga atacaaagag
     1441 ctgctggatc agatcgcgga actgttgcgt attcttggta gcgccgatcg tctgatggaa
     1501 gtgatccgtg aagagctgga gctggttcgt gaacagttcg gtgacaaacg tcgtactgaa
     1561 atcaccgcca acagcgcaga catcaacctg gaagatctga tcacccagga agatgtggtc
     1621 gtgacgctct ctcaccaggg ctacgttaag tatcagccgc tttctgaata cgaagcgcag
     1681 cgtcgtggcg ggaaaggtaa atctgccgca cgtattaaag aagaagactt tatcgaccga
     1741 ctgctggtgg cgaacactca cgaccatatt ctgtgcttct ccagccgtgg tcgcgtctat
     1801 tcgatgaaag tttatcagtt gccggaagcc actcgtggcg cgcgcggtcg tccgatcgtc
     1861 aacctgctgc cgctggagca ggacgaacgt atcactgcga tcctgccagt gaccgagttt
     1921 gaagaaggcg tgaaagtctt catggcgacc gctaacggta ccgtgaagaa aactgtcctc
     1981 accgagttca accgtctgcg taccgccggt aaagtggcga tcaaactggt tgacggcgat
     2041 gagctgatcg gcgttgacct gaccagcggc gaagacgaag taatgctgtt ctccgctgaa
     2101 ggtaaagtgg tgcgctttaa agagtcttct gtccgtgcga tgggctgcaa caccaccggt
     2161 gttcgcggta ttcgcttagg tgaaggcgat aaagtcgtct ctctgatcgt gcctcgtggc
     2221 gatggcgcaa tcctcaccgc aacgcaaaac ggttacggta aacgtaccgc agtggcggaa
     2281 tacccaacca agtcgcgtgc gacgaaaggg gttatctcca tcaaggttac cgaacgtaac
     2341 ggtttagttg ttggcgcggt acaggtagat gactgcgacc agatcatgat gatcaccgat
     2401 gccggtacgc tggtacgtac tcgcgtttcg gaaatcagca tcgtgggccg taacacccag
     2461 ggcgtgatcc tcatccgtac tgcggaagat gaaaacgtag tgggtctgca acgtgttgct
     2521 gaaccggttg acgaggaaga tctggatacc atcgacggca gtgccgcgga aggggacgat
     2581 gaaatcgctc cggaagtgga cgttgacgac gagccagaag aagaataa
//