LOCUS       pGEMHE-MmCENP-W        3301 bp DNA     circular SYN 28-JAN-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 3301)
  AUTHORS   Covid-19 WFH
  TITLE     Direct Submission
  JOURNAL   Exported Mar 24, 2025 from SnapGene 7.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3301
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     promoter        complement(63..167)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     551..570
                     /label=QCH_seq_primer_1
                     /note="color: black; sequence: CCTCTTCGCTATTACGCCAG"
     primer_bind     641..657
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     promoter        664..682
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     5'UTR           714..756
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     complement(758..778)
                     /label=Vector-W-R
                     /note="color: black; sequence: ctagcaccggggaattgatct; 
                     added: 2023-01-28"
     primer_bind     764..793
                     /label=InFu-MmW-F
                     /note="color: black; sequence: 
                     attccccggtgctagATGGCGCCCTCCACC; added: 2023-01-28"
     misc_feature    779..1036
                     /label=Mm_CENP-W
                     /note="color: #ffff00"
     primer_bind     complement(1011..1051)
                     /label=InFu-MmW-R
                     /note="color: black; sequence: 
                     TTAGTGGTAACCTCAACCTCTGCTCTTCTTCAGAATTACCT; added: 
                     2023-01-28"
     primer_bind     1037..1068
                     /label=Vector-W-F
                     /note="color: black; sequence: 
                     TGAGGTTACCACTAAACCAGCCTCAAGAACAC; added: 2023-01-28"
     promoter        complement(1281..1299)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     complement(1317..1333)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    1341..1357
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(join(1365..1371,1372..1389,1390..1395))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 1365 .. 1371 / #ffffff / -10
                        2: 1372 .. 1389 / #ffffff
                        3: 1390 .. 1395 / #ffffff / -35"
     protein_bind    1410..1431
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     rep_origin      complement(1719..2308)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
ORIGIN
        1 aaacaggaag gcaaatgccg aaaaaaggga ataagggcga cacggaaatg ttgaatactc
       61 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
      121 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
      181 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
      241 cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
      301 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
      361 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca
      421 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
      481 agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
      541 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      601 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa
      661 ttgtaatacg actcactata gggcgaatta attcgagctc ggtacccagc ttgcttgttc
      721 tttttgcaga agctcagaat aaacgctcaa ctttggcaga tcaattcccc ggtgctagat
      781 ggcgccctcc accacagtca ctaggcgggt aaagcgcaag gcgccccgcg ccttcctcaa
      841 gcgcacctta aagcagaaga agcctcacct aggtctgggg aggtgctgcg acctcctgat
      901 ccatttgaat tgcctacttt ttattcagcg attggcagaa gagtccagga caaatgcttg
      961 tgaaagtaaa tctagagtta tcaaaaagga tcatgtactg gctgcaggaa aggtaattct
     1021 gaagaagagc agaggttgag gttaccacta aaccagcctc aagaacaccc gaatggagtc
     1081 tctaagctac ataataccaa cttacacttt acaaaatgtt gtcccccaaa atgtagccat
     1141 tcgtatctgc tcctaataaa aagaaagttt cttcacattc taaaaaaaaa aaaaaaaaaa
     1201 aaaaaaaaaa aaaaaccccc cccccccccc cctgcaggcg cgccgccggc gatttaaatt
     1261 aattaatcta gcttgagtat tctatagtgt cacctaaata gcttggcgta atcatggtca
     1321 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     1381 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     1441 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     1501 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     1561 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     1621 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     1681 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     1741 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     1801 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     1861 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     1921 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     1981 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     2041 gtaagacacg accttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     2101 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     2161 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     2221 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     2281 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     2341 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     2401 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     2461 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     2521 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     2581 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     2641 agatttatca gcaataaacc agccagcccg gaagggccga gcgcagaagt ggtcctgcaa
     2701 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     2761 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     2821 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     2881 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     2941 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     3001 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     3061 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     3121 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     3181 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     3241 gcatctttta ctttcaccag cgtttctggg tgagcccttt caccagcgtt tctgggtgag
     3301 c
//
