LOCUS       pIF_Retron_Edito       11472 bp    DNA     circular SYN 02-AUG-2024
DEFINITION  pUFv2-3.
ACCESSION   pUFv2-3
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11472)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 25, 2025 from SnapGene 8.0.2
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     source          1..11472
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
                     /note="color: #ffff00"
     polyA_signal    complement(786..897)
                     /label=bGH poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
                     /note="color: #a6acb3; direction: LEFT"
     CDS             complement(931..978)
                     /codon_start=1
                     /label=nucleoplasmin NLS
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="KRPAATKKAGQAKKKK"
     CDS             complement(979..3477)
                     /codon_start=1
                     /label=Cas9(C)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="KPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEI
                     SGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTY
                     AHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIH
                     DDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPE
                     NIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYL
                     QNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEV
                     VKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQI
                     LDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVV
                     GTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITL
                     ANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESIL
                     PKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIME
                     RSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALP
                     SKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLD
                     KVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATL
                     IHQSITGLYETRIDLSQLGGD"
     CDS             complement(3478..5079)
                     /codon_start=1
                     /label=Cas9(N)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMR"
     intron          complement(5217..5444)
                     /label=hybrid intron
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=intron"
                     /note="color: #------; direction: LEFT"
     promoter        complement(5445..5727)
                     /label=chicken beta-actin promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=98.2"
                     /note="/match_length=101.8"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: LEFT"
     enhancer        complement(5729..6014)
                     /label=CMV enhancer
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=enhancer"
                     /note="color: #a6acb3; direction: LEFT"
     promoter        complement(6279..6488)
                     /label=H1 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=97.7"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: LEFT"
     misc_RNA        complement(6506..6581)
                     /label=gRNA scaffold
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_RNA"
                     /note="color: #a6acb3; direction: LEFT"
     promoter        complement(6630..6865)
                     /label=U6 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.6"
                     /note="/match_length=97.9"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: LEFT"
     enhancer        7324..7609
                     /label=CMV enhancer
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=enhancer"
                     /note="color: #a6acb3"
     promoter        7611..7892
                     /label=chicken beta-actin promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=98.6"
                     /note="/match_length=101.4"
                     /note="/other=promoter"
                     /note="color: #ffffff"
     intron          7893..8120
                     /label=hybrid intron
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=intron"
                     /note="color: #------"
     polyA_signal    9197..9308
                     /label=bGH poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
                     /note="color: #a6acb3"
     repeat_region   9413..9448
                     /label=AAV2 ITR (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=25.5"
                     /note="/other=repeat_region"
                     /note="color: #a6acb3"
     repeat_region   9449..9526
                     /label=AAV2 ITR (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=55.3"
                     /note="/other=repeat_region"
                     /note="color: #a6acb3"
     misc_feature    complement(9531..9582)
                     /label=bom (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=36.9"
                     /note="/other=misc_feature"
                     /note="color: #a6acb3; direction: LEFT"
     rep_origin      9601..10056
                     /label=f1 ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=98.9"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
                     /note="color: #ffff00"
     misc_feature    complement(10057..10106)
                     /label=bom (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=35.5"
                     /note="/other=misc_feature"
                     /note="color: #a6acb3; direction: LEFT"
     CDS             complement(10207..10243)
                     /codon_start=1
                     /label=rop (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=19.3"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="PRAFR**R*KPL"
     promoter        10337..10441
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff"
     CDS             10442..11302
                     /codon_start=1
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /note="color: #ccffcc"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tttagtctat
      661 cctcaatgtc taggggttcc tgcggccgct ccccagcatg cctgctattc tcttcccaat
      721 cctccccctt gctgtcctgc cccaccccac cccccagaat agaatgacac ctactcagac
      781 aatgcgatgc aatttcctca ttttattagg aaaggacagt gggagtggca ccttccaggg
      841 tcaaggaagg cacgggggag gggcaaacaa cagatggctg gcaactagaa ggcacagtcg
      901 aggctctcta ggaattcgtc ttcctcatca ctttttcttt tttgcctggc cggccttttt
      961 cgtggccgcc ggccttttgt cgcctcccag ctgagacagg tcgatccgtg tctcgtacag
     1021 gccggtgatg ctctggtgga tcagggtggc gtccagcacc tctttggtgc tggtgtacct
     1081 cttccggtcg atggtggtgt caaagtactt gaaggcggca ggggctccca gattggtcag
     1141 ggtaaacagg tggatgatat tctcggcctg ctctctgatg ggcttatccc ggtgcttgtt
     1201 gtaggcggac agcactttgt ccagattagc gtcggccagg atcactctct tggagaactc
     1261 gctgatctgc tcgatgatct cgtccaggta gtgcttgtgc tgttccacaa acagctgttt
     1321 ctgctcatta tcctcggggg agcccttcag cttctcatag tggctggcca ggtacaggaa
     1381 gttcacatat ttggagggca gggccagttc gtttcccttc tgcagttcgc cggcagaggc
     1441 cagcattctc ttccggccgt tttccagctc gaacagggag tacttaggca gcttgatgat
     1501 caggtccttt ttcacttctt tgtagccctt ggcttccaga aagtcgatgg gattcttctc
     1561 gaagctgctt ctttccatga tggtgatccc cagcagctct ttcacactct tcagtttctt
     1621 ggacttgccc ttttccactt tggccaccac cagcacagaa taggccacgg tggggctgtc
     1681 gaagccgccg tacttcttag ggtcccagtc cttctttctg gcgatcagct tatcgctgtt
     1741 cctcttgggc aggatagact ctttgctgaa gccgcctgtc tgcacctcgg tctttttcac
     1801 gatattcact tggggcatgc tcagcacttt ccgcacggtg gcaaaatccc ggcccttatc
     1861 ccacacgatc tccccggttt cgccgtttgt ctcgatcaga ggccgcttcc ggatctcgcc
     1921 gttggccagg gtaatctcgg tcttgaaaaa gttcatgatg ttgctgtaga agaagtactt
     1981 ggcggtagcc ttgccgattt cctgctcgct cttggcgatc atcttccgca cgtcgtacac
     2041 cttgtagtcg ccgtacacga actcgctttc cagcttaggg tactttttga tcagggcggt
     2101 tcccacgacg gcgttcaggt aggcgtcgtg ggcgtggtgg tagttgttga tctcgcgcac
     2161 tttgtaaaac tggaaatcct tccggaaatc ggacaccagc ttggacttca gggtgatcac
     2221 tttcacttcc cggatcagct tgtcattctc gtcgtactta gtgttcatcc gggagtccag
     2281 gatctgtgcc acgtgctttg tgatctgccg ggtttccacc agctgtctct tgatgaagcc
     2341 ggccttatcc agttcgctca ggccgcctct ctcggccttg gtcagattgt cgaactttct
     2401 ctgggtaatc agcttggcgt tcagcagctg ccgccagtag ttcttcatct tcttcacgac
     2461 ctcttcggag ggcacgttgt cgctcttgcc ccggttcttg tcgcttctgg tcagcacctt
     2521 gttgtcgatg gagtcgtcct tcagaaagct ctgaggcacg atatggtcca catcgtagtc
     2581 ggacagccgg ttgatgtcca gttcctggtc cacgtacata tcccgcccat tctgcaggta
     2641 gtacaggtac agcttctcgt tctgcagctg ggtgttttcc acggggtgtt ctttcaggat
     2701 ctggctgccc agctctttga tgccctcttc gatccgcttc attctctcgc ggctgttctt
     2761 ctgtcccttc tgggtggtct ggttctctct ggccatttcg atcacgatgt tctcgggctt
     2821 gtgccggccc atcactttca cgagctcgtc caccaccttc actgtctgca ggatgccctt
     2881 cttaatggcg gggctgccgg ccagattggc aatgtgctcg tgcaggctat cgccctggcc
     2941 ggacacctgg gctttctgga tgtcctcttt aaaggtcagg ctgtcgtcgt ggatcagctg
     3001 catgaagttt ctgttggcga agccgtcgga cttcaggaaa tccaggattg tcttgccgga
     3061 ctgcttgtcc cggatgccgt tgatcagctt ccggctcagc ctgccccagc cggtgtatct
     3121 ccgccgcttc agctgcttca tcactttgtc gtcgaacagg tgggcatagg ttttcagccg
     3181 ttcctcgatc atctctctgt cctcaaacag tgtcagggtc agcacgatat cttccagaat
     3241 gtcctcgttt tcctcattgt ccaggaagtc cttgtccttg ataattttca gcagatcgtg
     3301 gtatgtgccc agggaggcgt tgaaccgatc ttccacgccg gagatttcca cggagtcgaa
     3361 gcactcgatt ttcttgaagt agtcctcttt cagctgcttc acggtcactt tccggttggt
     3421 cttgaacagc aggtccacga tggccttttt ctgctcgccg ctcaggaagg cgggctttct
     3481 cattccctcg gtcacgtatt tcactttggt cagctcgtta tacacggtga agtactcgta
     3541 cagcaggctg tgcttgggca gcaccttctc gttgggcagg ttcttatcga agttggtcat
     3601 ccgctcgatg aagctctggg cggaagcgcc cttgtccacc acttcctcga agttccaggg
     3661 ggtgatggtt tcctcgctct ttctggtcat ccaggcgaat ctgctgtttc ccctggccag
     3721 agggcccacg tagtagggga tgcggaaggt caggatcttc tcgatctttt cccggttgtc
     3781 cttcaggaat gggtaaaaat cttcctgccg ccgcagaatg gcgtgcagct ctcccaggtg
     3841 gatctggtgg gggatgctgc cgttgtcgaa ggtccgctgc ttccgcagca ggtcctctct
     3901 gttcagcttc acgagcagtt cctcggtgcc gtccatcttt tccaggatgg gcttgatgaa
     3961 cttgtagaac tcttcctggc tggctccgcc gtcaatgtag ccggcgtagc cgttcttgct
     4021 ctggtcgaag aaaatctctt tgtacttctc aggcagctgc tgccgcacga gagctttcag
     4081 cagggtcagg tcctggtggt gctcgtcgta tctcttgatc atagaggcgc tcaggggggc
     4141 cttggtgatc tcggtgttca ctctcaggat gtcgctcagc aggatggcgt cggacaggtt
     4201 cttggcggcc agaaacaggt cggcgtactg gtcgccgatc tgggccagca ggttgtccag
     4261 gtcgtcgtcg taggtgtcct tgctcagctg cagtttggca tcctcggcca ggtcgaagtt
     4321 gctcttgaag ttgggggtca ggcccaggct cagggcaatc aggtttccga acaggccatt
     4381 cttcttctcg ccgggcagct gggcgatcag attttccagc cgtctgctct tgctcagtct
     4441 ggcagacagg atggccttgg cgtccacgcc gctggcgttg atggggtttt cctcgaacag
     4501 ctggttgtag gtctgcacca gctggatgaa cagcttgtcc acgtcgctgt tgtcggggtt
     4561 caggtcgccc tcgatcagga agtggccccg gaacttgatc atgtgggcca gggccagata
     4621 gatcagccgc aggtcggcct tgtcggtgct gtccaccagt ttctttctca ggtggtagat
     4681 ggtggggtac ttctcgtggt aggccacctc gtccacgatg ttgccgaaga tggggtgccg
     4741 ctcgtgcttc ttatcctctt ccaccaggaa ggactcttcc agtctgtgga agaagctgtc
     4801 gtccaccttg gccatctcgt tgctgaagat ctcttgcaga tagcagatcc ggttcttccg
     4861 tctggtgtat cttcttctgg cggttctctt cagccgggtg gcctcggctg tttcgccgct
     4921 gtcgaacagc agggctccga tcaggttctt cttgatgctg tgccggtcgg tgttgcccag
     4981 caccttgaat ttcttgctgg gcaccttgta ctcgtcggtg atcacggccc agcccacaga
     5041 gttggtgccg atgtccaggc cgatgctgta cttcttgtcg gctgctggga ctccgtggat
     5101 accgaccttc cgcttcttct ttggggccat cttatcgtca tcgtctttgt aatcaatatc
     5161 atgatccttg tagtctccgt cgtggtcctt atagtccatg gtggcagaag acggaactga
     5221 aaaaaagtga tttcaggcag gtgctccagg taattaaaca ttaatacccc accaaccaac
     5281 catcccttaa acccttacct cttgctcagc taattacagc ccggaggaga agggccgtcc
     5341 cgcccgctca cctgtgggag taacgcggtc agtcagagcc ggggcgggcg gcgcgaggcg
     5401 gcggcggagc ggggcacggg gcgaaggcag cgcgcagcga ctcccgcccg ccgcgcgctt
     5461 cgctttttat agggccgccg ccgccgccgc ctcgccataa aaggaaactt tcggagcgcg
     5521 ccgctctgat tggctgccgc cgcacctctc cgcctcgccc cgccccgccc ctcgccccgc
     5581 cccgccccgc ctggcgcgcg cccccccccc cccccccccg cccccatcgc tgcacaaaat
     5641 aattaaaaaa taaataaata caaaattggg ggtggggagg ggggggggag atggggagag
     5701 tgaagcagaa cgtggggctc acctcgacca tggtaatagc gatgactaat acgtagatgt
     5761 actgccaagt aggaaagtcc cataaggtca tgtactgggc acaatgccag gcgggccatt
     5821 taccgtcatt gacgtcaata gggggcgtac ttggcatatg atacacttga tgtactgcca
     5881 agtgggcagt ttaccgtaaa tactccaccc attgacgtca atggaaagtc cctattggcg
     5941 ttactattga cgtcaatggg cgggggtcgt tgggcggtca gccaggcggg ccatttaccg
     6001 taagttatgt aacgcgagac ctgctacgta acgggagggt cgctagaaaa aaacgccaac
     6061 agcggcaata ccatcaaacc attacaaacg gcagaagctg gaggaggaag ggcctgagtc
     6121 cgagcagaag aaagcatgta gcgaagggct cccatcacat caaccggtgg cgcattgcca
     6181 cgaagcaggc caactggcgt taagtcggcg actcgccgac cctcccggca caaaaggccg
     6241 gaaacgctat tgccactgct ggcgggggat ctgtggtcct catacagaac ttataagatt
     6301 cccaaatcca aagacatttc acgtttatgg tgatttccca gaacacatag cgacatgcaa
     6361 atattgcagg gcgccactcc cctgtccctc acagccatct tcctgccagg gcgcacgcgc
     6421 gctgggtgtt cccgcctagt gacactgggc ccgcgattcc ttggagcggg ttgatgacgt
     6481 cagcgttccg tacgctagaa aaaaagcacc gactcggtgc cactttttca agttgataac
     6541 ggactagcct tattttaact tgctatttct agctctaaaa cttcttcttc tgctcggact
     6601 cggtgtttcg tcctggtgtt taggtctcct tccacaagat atataaagcc aagaaatcga
     6661 aatactttca agttacggta agcatatgat agtccatttt aaaacataat tttaaaactg
     6721 caaactaccc aagaaattat tactttctac gtcacgtatt ttgtactaat atctttgtgt
     6781 ttacagtcaa attaattcca attatctctc taacagcctt gtatcgtata tgcaaatatg
     6841 aaggaatcat gggaaatagg ccctcacatt acggggtctg acgctcagtg gaacgaaaac
     6901 tcacgttaag ggattttggt catgagatta tcaaaaagga tggcgacgaa tagcagttta
     6961 cttcctccct gcgggcccct cctgaggtgc cacctgtacg gctcgttgaa gccgattagt
     7021 acaatagatt tattcaaccc caaaggtcta cactcccggc ctactcttag ctgatatgtc
     7081 gcgtcgggcc ccgggtttga gtgcgttagc gccgggttgt ctggcccagt tcgacttctg
     7141 ggtacctcat cgggttacac tggctgacac tcatccttca gacagggttc ccttattggg
     7201 actagtaacc gaggttcgaa actatcccta cttccaatgg tctgcaccta gatcctttta
     7261 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     7321 agccgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
     7381 attgacgtca atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
     7441 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
     7501 tgacgtcaat gacggtaaat ggcccgcctg gcattgtgcc cagtacatga ccttatggga
     7561 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tcgaggtgag
     7621 ccccacgttc tgcttcactc tccccatctc cccccccctc cccaccccca attttgtatt
     7681 tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg ggggggcgcg
     7741 cgccaggcgg ggcggggcgg ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg
     7801 cagccaatca gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc
     7861 ggccctataa aaagcgaagc gcgcggcggg cgggagtcgc tgcgcgctgc cttcgccccg
     7921 tgccccgctc cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc gcgttactcc
     7981 cacaggtgag cgggcgggac ggcccttctc ctccgggctg taattagctg agcaagaggt
     8041 aagggtttaa gggatggttg gttggtgggg tattaatgtt taattacctg gagcacctgc
     8101 ctgaaatcac tttttttcag gttggaagag cgccaccatg cctaagaaaa agagaaaggt
     8161 ggacgccacc agaatcacac tgctggccct tgacctgttt ggcagccctg gctggtccgc
     8221 cgataaggaa atccagaggc tgtatgccct gagcaatcac accggcagac actaccggag
     8281 aatcatcctg agcaagaggc atggcggcca gagactggtg ctggctcctg attacctgct
     8341 gaaaaccgtg cagcggaaca ttctgaaaaa cgtgctgtcc cagttccccc tgtctagctt
     8401 cgccacagct tacagacctg gatgtccaat cgtgtctaat gcccagcccc actgtcagca
     8461 gcctcagatc ctgaagttcg acatcgagaa cttcttcgac agcatctcct ggctgcaagt
     8521 gtggcgggtg ttccggcagg ctcagctgcc cagaaacgtg gtcacaatgc tgacctggct
     8581 gtgctgctac aacgacgccc tgcctcaagg cgccccaaca agccccgcca tctctaatct
     8641 ggtgatgtgc agattcgatg agagaatcgg agaatggtgc caagctcggg gcatcaccta
     8701 cacccgctac tgcgacgaca tgaccttcag cggccacttc aacgccagac tggttaagaa
     8761 caaggtgtgc ggcctgctcg ccgagctggg cctgaacctg aaccagagaa agagctgcct
     8821 ggtggccgcc tgcaagcggc aacaggtgac cggcatcgtg gtgaaccaca agcctcagct
     8881 ggcccgggaa gccagacggg ccctgagaca ggaggtgcac ctctgtcaga aatacggcgt
     8941 gatcagccac ctgagccaca gaggtgagct ggacagctct ggagatctgc acgcccaggc
     9001 caccgcctac ctgtacgccc tgcagggcag aattaactgg ctgctgcaga tcaaccccga
     9061 ggacgagggc tttaagcagg ccagagaagg cgtgaagcgg atgctggtcg cctggaagcg
     9121 gcctgctgct acaaagaaag ccggccaggc caagaagaag aaatgagaat tccgctcttc
     9181 tagagctcgc tgatcactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     9241 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     9301 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     9361 agcaaggggg aggattggga agagaatagc aggcatgctg gggagcggcc gcaggaaccc
     9421 ctagtgatgg agttggccac tccctctcgg cgaccaaagg tcgcccgacg cccgggcttt
     9481 gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg
     9541 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag caaccatagt
     9601 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     9661 ctacacttgc cagcgcctta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     9721 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     9781 gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc
     9841 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     9901 gactcttgtt ccaaactgga acaacactca actctatctc gggctattct tttgatttat
     9961 aagggatttt gccgatttcg gtctattggt taaaaaatga gctgatttaa caaaaattta
    10021 acgcgaattt taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct
    10081 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
    10141 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgacattc tccggagctg
    10201 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggttaaagg gcctcgtgat
    10261 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
    10321 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
    10381 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
    10441 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
    10501 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
    10561 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
    10621 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
    10681 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
    10741 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
    10801 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
    10861 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
    10921 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
    10981 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
    11041 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
    11101 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtggaag
    11161 ccgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
    11221 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
    11281 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
    11341 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
    11401 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
    11461 caaaggatct tc
//
