LOCUS       Exported                7428 bp ds-DNA     circular SYN 23-SEP-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7428)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Mar 25, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7428
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..141
                     /label=L-ITR
                     /note="/vntifkey=21"
     promoter        156..1419
                     /label=EF-1a
                     /note="/vntifkey=29"
     misc_feature    1426..1459
                     /label=F3
                     /note="/vntifkey=21"
     misc_feature    1510..1543
                     /label=F5
                     /note="/vntifkey=21"
     misc_feature    1544..1551
                     /label=AscI
     misc_feature    1552..1854
                     /label=10XMyc
     misc_feature    1932..1937
                     /label=SalI
     misc_feature    1938..1971
                     /label=lox2722
                     /note="/vntifkey=21"
     misc_feature    2022..2055
                     /label=loxN
                     /note="/vntifkey=21"
     misc_feature    2062..2142
                     /label=CMV intron branch point
                     /label=CMV\intron\branch\point
                     /note="/vntifkey=21"
     misc_feature    2143..2158
                     /label=CMV intron acceptor
                     /label=CMV\intron\acceptor
                     /note="/vntifkey=21"
     misc_feature    2159..2867
                     /label=Synaptophysin Ex2
                     /label=Synaptophysin\Ex2
                     /note="/vntifkey=21"
     misc_feature    2868..2876
                     /label=Spacer
                     /note="/vntifkey=21"
     misc_feature    2877..3032
                     /label=YFP START
                     /label=YFP\START
                     /note="/vntifkey=21"
     misc_feature    3033..3046
                     /label=CE intron donor
                     /label=CE\intron\donor
                     /note="/vntifkey=21"
     misc_feature    complement(3053..3086)
                     /label=lox2722 Rev
                     /label=lox2722\Rev
                     /note="/vntifkey=21"
     misc_feature    complement(3137..3170)
                     /label=loxN Rev
                     /label=loxN\Rev
                     /note="/vntifkey=21"
     misc_feature    complement(3177..3191)
                     /label=CMV intron donor
                     /label=CMV\intron\donor
                     /note="/vntifkey=21"
     misc_feature    complement(3192..3400)
                     /label=Synaptophysin Ex1
                     /label=Synaptophysin\Ex1
                     /note="/vntifkey=21"
     misc_feature    complement(3413..3446)
                     /label=F3 Rev
                     /label=F3\Rev
                     /note="/vntifkey=21"
     misc_feature    complement(3497..3530)
                     /label=F5 Rev
                     /label=F5\Rev
                     /note="/vntifkey=21"
     misc_feature    3545..4153
                     /label=WPRE
                     /note="/vntifkey=21"
     polyA_signal    4173..4651
                     /label=hGH polyA
                     /label=hGH\polyA
                     /note="/vntifkey=25"
     misc_feature    4691..4831
                     /label=R-ITR
                     /note="/vntifkey=21"
     rep_origin      4923..5229
                     /label=f1 Origin
                     /label=f1\Origin
                     /note="/vntifkey=33"
     misc_feature    5748..6605
                     /label=AmpR
                     /note="/vntifkey=21"
     rep_origin      6756..7423
                     /label=pUC ori
                     /label=pUC\ori
                     /note="/vntifkey=33"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtaagct ttgcaaagat ggataaagtt
      181 ttaaacagag aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgggaat
      241 tggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg
      301 gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag
      361 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
      421 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc
      481 cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat
      541 tacttccact ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      601 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      661 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      721 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      781 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      841 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      901 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      961 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
     1021 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
     1081 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
     1141 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
     1201 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1261 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1321 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1381 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gatccgaagt tcctattctt
     1441 caaatagtat aggaacttcg cagaatggta gctggattgt agctgctatt agcaatatga
     1501 aacctcttag aagttcctat tcttcaaaag gtataggaac ttcggcgcgc cctagaggtc
     1561 ctcctcgcta atcaatttct gcccaagatc ttcttcactt attaattttt gtcccaagtc
     1621 ctcttccgaa atcagttttt ggcctaaatc ttcctctgat attagcttct gacccaggtc
     1681 ttcttcggag ataagttttt gccctagatc ctcctcagaa atcaacttct gtccgaggtc
     1741 ttcctcgctt attaacttct ggccaagatc ctcttcacta atcagttttt gacccaagtc
     1801 ttcctccgat attagcttct gccctaaatc ctcttctgag atgagttttt gtcccacggg
     1861 cagctgtggg acgacatgac ttaaccagtt aaccatgttc tctgaacatg agaagggctg
     1921 gccactctcc agtcgacata acttcgtata ggatacttta tacgaagtta tgcagaatgg
     1981 tagctggatt gtagctgcta ttagcaatat gaaacctctt aataacttcg tatagtatac
     2041 cttatacgaa gttatactag ttgtgccccc cctttttttt ataaaattgt attaatgtta
     2101 tatacatatc tcctgtatgt gacccatgtg cttatgactc tatttctcat gtgtttaggc
     2161 tgcaccaagt gtactttgat gcaccctcct gcgtcaaagg gggcactacc aagatcttcc
     2221 tggttgggga ctactcctcg tcggctgaat tctttgtcac cgtggctgtg tttgccttcc
     2281 tctactccat gggggccctg gccacctaca tcttcctgca gaacaagtac cgagagaaca
     2341 acaaagggcc tatgatggac tttctggcta cagccgtgtt cgctttcatg tggctagtta
     2401 gttcatcagc ctgggccaaa ggcctgtccg atgtgaagat ggccacggac ccagagaaca
     2461 ttatcaagga gatgcccatg tgccgccaga cagggaacac atgcaaggaa ctgagggacc
     2521 ctgtgacttc aggactcaac accacagtgg tgtttggctt cctgaacctg gtgctctggg
     2581 ttggcaactt atggttcgtg ttcaaggaga caggctgggc agccccattc atgcgcgcac
     2641 ctccaggcgc cccggaaaag caaccagcac ctggcgatgc ctacggcgat gcgggctacg
     2701 ggcagggccc cggaggctat gggccccagg actcctacgg gcctcagggt ggttatcaac
     2761 ccgattacgg gcagccagcc agcggtggcg gtggctacgg gcctcagggc gactatgggc
     2821 agcaaggcta tggccaacag ggtgcgccca cctccttctc caatcaggcg gccgccgtga
     2881 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     2941 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     3001 tgaccctgaa gttcatctgc accaccggca aggtgagtac aggaggggta ccataacttc
     3061 gtataaagta tcctatacga agttatttgc cttaacccag aaattatcac tgttattctt
     3121 tagaatggtg caaagaataa cttcgtataa ggtatactat acgaagttat gctagcaacc
     3181 ccgacacgta cctgaagggg tactcgaatt caacttcgat gttgagggca ctctccgtct
     3241 tgttggcaca ctccacgctc agccgaagct ccccggtgta gctgccacac gtagcaaagg
     3301 cgaagatggc aaagacccac tgcagcacct tcacgaagcc aaggggctcc ttgaccaccc
     3361 ggaactgacc cccagccacc agctgattca ccacgtccat ggtggctcta gagaagttcc
     3421 tatactattt gaagaatagg aacttcttgc cttaacccag aaattatcac tgttattctt
     3481 tagaatggtg caaagagaag ttcctatacc ttttgaagaa taggaacttc gaattcgata
     3541 tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     3601 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     3661 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     3721 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     3781 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     3841 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     3901 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     3961 ttccttggct gctcgcctat gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     4021 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     4081 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     4141 cgcatcgata ccgagcgctg ctcgagagat ctacgggtgg catccctgtg acccctcccc
     4201 agtgcctctc ctggccctgg aagttgccac tccagtgccc accagccttg tcctaataaa
     4261 attaagttgc atcattttgt ctgactaggt gtccttctat aatattatgg ggtggagggg
     4321 ggtggtatgg agcaaggggc aagttgggaa gacaacctgt agggcctgcg gggtctattg
     4381 ggaaccaagc tggagtgcag tggcacaatc ttggctcact gcaatctccg cctcctgggt
     4441 tcaagcgatt ctcctgcctc agcctcccga gttgttggga ttccaggcat gcatgaccag
     4501 gctcagctaa tttttgtttt tttggtagag acggggtttc accatattgg ccaggctggt
     4561 ctccaactcc taatctcagg tgatctaccc accttggcct cccaaattgc tgggattaca
     4621 ggcgtgaacc actgctccct tccctgtcct tctgattttg taggtaacca cgtgcggacc
     4681 gagcggccgc aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg
     4741 ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca
     4801 gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt tctccttacg
     4861 catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg
     4921 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     4981 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     5041 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     5101 tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga
     5161 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     5221 ctggaacaac actcaaccct atctcgggct attcttttga tttataaggg attttgccga
     5281 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     5341 aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct gatgccgcat
     5401 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     5461 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     5521 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
     5581 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     5641 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     5701 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     5761 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     5821 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     5881 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     5941 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     6001 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     6061 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     6121 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     6181 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     6241 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     6301 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     6361 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     6421 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     6481 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     6541 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     6601 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     6661 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     6721 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     6781 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     6841 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     6901 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     6961 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     7021 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     7081 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     7141 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     7201 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     7261 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     7321 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     7381 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgt
//
