LOCUS       Exported                8236 bp ds-DNA     circular SYN 24-MAR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8236)
  AUTHORS   gonatopoulospt
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Mar 25, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8236
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        7..507
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     CDS             529..1128
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(532..550)
                     /label=Puro rev seq1
     primer_bind     1129..1151
                     /label=Infus_vec_F
     misc_feature    1144..1732
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1197..1217)
                     /label=Quintara_WPRE-R
     primer_bind     complement(1197..1217)
                     /label=QB0112
     CDS             complement(1615..1626)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     1695..1714
                     /label=WPRE-F
     LTR             1804..2037
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(1809..1828)
                     /label=3'_LTR-R
     polyA_signal    2115..2236
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2152..2171)
                     /label=Quintara_SV40pA-R
     primer_bind     complement(2152..2171)
                     /label=QB0102
     primer_bind     2206..2225
                     /label=Quintara_EBV Reverse
     primer_bind     2206..2225
                     /label=QB0017
     rep_origin      2276..2411
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     terminator      2331..2338
                     /label=Transcription termination
     primer_bind     2338..2357
                     /label=Quintara_SV40pro-F
     primer_bind     2338..2357
                     /label=QB0103
     primer_bind     complement(2361..2372)
                     /label=Quintara_SV40pro-F
     primer_bind     complement(2361..2372)
                     /label=QB0103
     primer_bind     complement(2431..2450)
                     /label=Quintara_T7
     primer_bind     complement(2431..2450)
                     /label=QB0106
     promoter        complement(2432..2450)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2460..2477)
                     /label=Quintara_M13 (-21) Forward
     primer_bind     complement(2460..2477)
                     /label=QB0046
     primer_bind     complement(2460..2476)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2469..2491)
                     /label=Quintara_M13/pUC Forward
     primer_bind     complement(2469..2491)
                     /label=QB0049
     primer_bind     complement(2480..2496)
                     /label=Quintara_M13 (-40)
     primer_bind     complement(2480..2496)
                     /label=QB0047
     rep_origin      2618..3073
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2705..2724)
                     /label=F1ori-R
     promoter        3099..3203
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3146..3167)
                     /label=AmpR-pro_R
     CDS             3204..4064
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3422..3441)
                     /label=Quintara_Amp-R
     primer_bind     complement(3422..3441)
                     /label=QB0005
     rep_origin      4235..4823
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4249..4268)
                     /label=pBR322ori-R
     primer_bind     4456..4475
                     /label=Quintara_RepO-R
     primer_bind     4456..4475
                     /label=QB0767
     primer_bind     complement(4582..4600)
                     /label=Quintara_RepO-F
     primer_bind     complement(4582..4600)
                     /label=QB0766
     primer_bind     4977..4994
                     /label=Quintara_L4440
     primer_bind     4977..4994
                     /label=QB0039
     protein_bind    5111..5132
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5147..5177
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5185..5201
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5189..5212
                     /label=Quintara_Tac promoter
     primer_bind     5189..5212
                     /label=QB0108
     primer_bind     5190..5212
                     /label=Quintara_M13/pUC Reverse
     primer_bind     5190..5212
                     /label=QB0050
     primer_bind     5209..5225
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5209..5225
                     /label=Quintara_M13 Reverse
     primer_bind     5209..5225
                     /label=QB0048
     primer_bind     5244..5264
                     /label=Quintara_T3
     primer_bind     5244..5264
                     /label=QB0105
     promoter        5246..5264
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5292..5518
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5519..5699
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5746..5871
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     primer_bind     5754..5771
                     /label=HIV-1-phi_F
     primer_bind     complement(5837..5859)
                     /label=HIV-1-phi_R
     misc_feature    6364..6597
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     primer_bind     6404..6423
                     /label=RRE_F
     primer_bind     complement(6520..6539)
                     /label=RRE_R
     CDS             6782..6826
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     6931..6951
                     /label=pLCKO_3'_fwd
     misc_feature    6943..7041
                     /label=TWIST Standard 3' Padding Sequence_6 T's
     primer_bind     7015..7035
                     /label=DS_polyT_R
     misc_feature    complement(7042..7078)
                     /label=tevopreQ1
     misc_feature    complement(7079..7100)
                     /label=10x Genomics CS2
     misc_feature    7105..7110
                     /label=BveI/BfuAI(1)
                     /label=BfuAI(1)
                     /note="ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 
                     0} width 5 offset 0
                     ApEinfo_revcolor=green
                     ApEinfo_label=BfuAI
                     ApEinfo_fwdcolor=#fa0810"
     CDS             7439..7496
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLVCG"
     misc_feature    7486
                     /label=Insertion confirmed by PlasmidExpress & Sanger!
     CDS             7497..7745
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="VQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMM
                     TTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     7624..7643
                     /label=Quintara_GW-3'
     primer_bind     7624..7643
                     /label=QB0030
     misc_feature    7746..7751
                     /label=BveI/BfuAI
                     /label=BfuAI
                     /note="ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 
                     0} width 5 offset 0
                     ApEinfo_revcolor=green
                     ApEinfo_fwdcolor=#fa101d"
     misc_feature    complement(7756..8005)
                     /label=U6 promoter starting with G
     misc_feature    7756..7830
                     /label=TWIST Standard 5' Padding Sequence
     primer_bind     complement(7812..7833)
                     /label=U6_fwd
     primer_bind     complement(7815..7834)
                     /label=Quintara_LKO.1 5'
     primer_bind     complement(7815..7834)
                     /label=QB0042
     misc_feature    8047..8164
                     /label=cPPT
                     /note="ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 
                     0} width 5 offset 0
                     ApEinfo_revcolor=green
                     ApEinfo_fwdcolor=#1efa7b"
     primer_bind     complement(8071..8091)
                     /label=cPPT-CTS_R
     primer_bind     complement(8072..8090)
                     /label=HIV-1_pol_rev
     primer_bind     8077..8101
                     /label=Quintara_FLAP
     primer_bind     8077..8101
                     /label=cPPT-CTS_F
     primer_bind     8077..8101
                     /label=cPPT-CTS_F (Ethan)
     primer_bind     8077..8101
                     /label=QB0226
ORIGIN
        1 cccgggttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg
       61 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt
      121 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct
      181 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa
      241 gccgcagctc acactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc
      301 cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc
      361 cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc
      421 gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt
      481 gaccgaatca ccgacctctc tccccagggg gatccaccgg agcttaccat gaccgagtac
      541 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
      601 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
      661 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
      721 tgggtcgcgg acgacggcgc cgccgtggcg gtctggacca cgccggagag cgtcgaagcg
      781 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
      841 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
      901 ctggccaccg tcggtgtttc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
      961 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
     1021 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
     1081 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaac gcgttaagtc
     1141 gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     1201 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     1261 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     1321 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     1381 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     1441 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     1501 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     1561 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     1621 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     1681 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcgtcgactt
     1741 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     1801 gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
     1861 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     1921 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     1981 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
     2041 cgtatagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     2101 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     2161 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     2221 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc
     2281 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     2341 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     2401 ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca
     2461 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     2521 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     2581 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
     2641 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     2701 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     2761 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     2821 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     2881 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     2941 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     3001 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     3061 acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     3121 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     3181 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     3241 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     3301 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     3361 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     3421 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     3481 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     3541 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     3601 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     3661 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     3721 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     3781 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     3841 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     3901 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     3961 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     4021 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     4081 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     4141 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     4201 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     4261 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     4321 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     4381 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     4441 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     4501 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     4561 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     4621 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     4681 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     4741 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     4801 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     4861 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     4921 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     4981 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     5041 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     5101 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     5161 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
     5221 atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc
     5281 tgcaagctta atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag
     5341 ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag
     5401 gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac
     5461 cactgaattg ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg
     5521 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     5581 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     5641 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     5701 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
     5761 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
     5821 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
     5881 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
     5941 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
     6001 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
     6061 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
     6121 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
     6181 aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga
     6241 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     6301 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     6361 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     6421 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     6481 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     6541 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     6601 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     6661 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     6721 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     6781 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     6841 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     6901 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     6961 attatcgttt cagacccacc tcccaacccc gaggggaccc tgcaggattt aaatccgagg
     7021 ggacccagag aattcaaaaa attctagttg gtttaacgcg taactagata gaaccgcgcc
     7081 ttagccgcta ataggtgagc gtaggcaggt gcttactaaa agccagataa cagtatgcgt
     7141 atttgcgcgc tgatttttgc ggtataagaa tatatactga tatgtatacc cgaagtatgt
     7201 caaaaagagg tatgctatga agcagcgtat tacagtgaca gttgacagcg acagctatca
     7261 gttgctcaag gcatatatga tgtcaatatc tccggtctgg taagcacaac catgcagaat
     7321 gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg gatggctgag
     7381 gtcgcccggt ttattgaaat gaacggctct tttgctgacg agaacagggg ctggtgaaat
     7441 gcagtttaag gtttacacct ataaaagaga gagccgttat cgtcttgttt gtggatgtac
     7501 agagtgatat tattgacacg cccgggcgac ggatggtgat ccccctggcc agtgcacgtc
     7561 tgctgtcaga taaagtctcc cgtgaacttt acccggtggt gcatatcggg gatgaaagct
     7621 ggcgcatgat gaccaccgat atggccagtg tgccggtctc cgttatcggg gaagaagtgg
     7681 ctgatctcag ccaccgcgaa aatgacatca aaaacgccat taacctgatg ttctggggaa
     7741 tataaacctg cagagcggta cctcgtcctt tccacaagat atataaagcc aagaaatcga
     7801 aatactttca agttacggta agcatatgat agtccatttt aaaacataat tttaaaactg
     7861 caaactaccc aagaaattat tactttctac gtcacgtatt ttgtactaat atctttgtgt
     7921 ttacagtcaa attaattcta attatctctc taacagcctt gtatcgtata tgcaaatatg
     7981 aaggaatcat gggaaatagg ccctcgaatt ctagatcttg agacaaatgg cagtattcat
     8041 ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     8101 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
     8161 ttttcgggtt tattacaggg acagcagaga tccactttgg cgccggctcg agatttaaat
     8221 cctgcaggcg aggggg
//
