LOCUS       pLCKO-CMV-TMPRSS2-IRES-Hygromycin        9866 bp    DNA     circular UNA 26-MAR-2025
DEFINITION  .
ACCESSION   urn.local...26u-iyqt4iz
VERSION     urn.local...26u-iyqt4iz
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     enhancer        1..380
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        381..584
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /label="CMV promoter"
     promoter        629..647
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             689..2167
                     /created_by="User"
                     /label="TMPRSS2"
     motif           2188..2758
                     /created_by="User"
                     /modified_by="u0063854"
                     /label="IRES"
     CDS             2791..3816
                     /created_by="User"
                     /modified_by="User"
                     /label="Hygro"
     domain          3834..4409
                     /Source="Geneious"
                     /label="WPRE"
     domain          4475..4490
                     /Source="Geneious"
                     /label="cPPT"
     domain          4545..4725
                     /Source="Geneious"
                     /label="HIV-1_5_LTR"
     terminator      4806..4925
                     /Source="Geneious"
                     /modified_by="u0063854"
                     /label="SV40_PA_terminator"
     domain          4943..5041
                     /Source="Geneious"
                     /label="SV40_origin"
     promoter        complement(5120..5138)
                     /Source="Geneious"
                     /modified_by="u0063854"
                     /label="T7_promoter"
     domain          5157..5305
                     /Source="Geneious"
                     /label="lacZ_a"
     domain          5323..5629
                     /Source="Geneious"
                     /label="f1_origin"
     promoter        5822..5850
                     /Source="Geneious"
                     /modified_by="u0063854"
                     /label="AmpR_promoter"
     domain          5892..6752
                     /Source="Geneious"
                     /label="Ampicillin"
     domain          6907..7526
                     /Source="Geneious"
                     /label="pBR322_origin"
     domain          8207..8387
                     /Source="Geneious"
                     /label="HIV-1_5_LTR"
     domain          8498..8542
                     /Source="Geneious"
                     /label="HIV-1_psi_pack"
     domain          9052..9285
                     /Source="Geneious"
                     /label="RRE"
     domain          9701..9818
                     /created_by="User"
                     /modified_by="User"
                     /label="central DNA flap"
     domain          9705..9720
                     /Source="Geneious"
                     /modified_by="User"
                     /label="cPPT"
     domain          9803..9814
                     /created_by="User"
                     /label="CTS"
ORIGIN      
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagcgttt aaacgggccc tctagaccat ggctttgaac tcagggtcac caccagctat
      721 tggaccttac tatgaaaacc atggatacca accggaaaac ccctatcccg cacagcccac
      781 tgtggtcccc actgtctacg aggtgcatcc ggctcagtac tacccgtccc ccgtgcccca
      841 gtacgccccg agggtcctga cgcaggcttc caaccccgtc gtctgcacgc agcccaaatc
      901 cccatccggg acagtgtgca cctcaaagac taagaaagca ctgtgcatca ccttgaccct
      961 ggggaccttc ctcgtgggag ctgcgctggc cgctggccta ctctggaagt tcatgggcag
     1021 caagtgctcc aactctggga tagagtgcga ctcctcaggt acctgcatca acccctctaa
     1081 ctggtgtgat ggcgtgtcac actgccccgg cggggaggac gagaatcggt gtgttcgcct
     1141 ctacggacca aacttcatcc ttcaggtgta ctcatctcag aggaagtcct ggcaccctgt
     1201 gtgccaagac gactggaacg agaactacgg gcgggcggcc tgcagggaca tgggctataa
     1261 gaataatttt tactctagcc aaggaatagt ggatgacagc ggatccacca gctttatgaa
     1321 actgaacaca agtgccggca atgtcgatat ctataaaaaa ctgtaccaca gtgatgcctg
     1381 ttcttcaaaa gcagtggttt ctttacgctg tatagcctgc ggggtcaact tgaactcaag
     1441 ccgccagagc aggattgtgg gcggcgagag cgcgctcccg ggggcctggc cctggcaggt
     1501 cagcctgcac gtccagaacg tccacgtgtg cggaggctcc atcatcaccc ccgagtggat
     1561 cgtgacagcc gcccactgcg tggaaaaacc tcttaacaat ccatggcatt ggacggcatt
     1621 tgcggggatt ttgagacaat ctttcatgtt ctatggagcc ggataccaag tagaaaaagt
     1681 gatttctcat ccaaattatg actccaagac caagaacaat gacattgcgc tgatgaagct
     1741 gcagaagcct ctgactttca acgacctagt gaaaccagtg tgtctgccca acccaggcat
     1801 gatgctgcag ccagaacagc tctgctggat ttccgggtgg ggggccaccg aggagaaagg
     1861 gaagacctca gaagtgctga acgctgccaa ggtgcttctc attgagacac agagatgcaa
     1921 cagcagatat gtctatgaca acctgatcac accagccatg atctgtgccg gcttcctgca
     1981 ggggaacgtc gattcttgcc agggtgacag tggagggcct ctggtcactt cgaagaacaa
     2041 tatctggtgg ctgatagggg atacaagctg gggttctggc tgtgccaaag cttacagacc
     2101 aggagtgtac gggaatgtga tggtattcac ggactggatt tatcgacaaa tgagggcaga
     2161 cggctaaggt accggatccg gaattccgcc cccccccccc ccccctaacg ttactggccg
     2221 aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc
     2281 gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag
     2341 gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt
     2401 tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa
     2461 ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc
     2521 aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg
     2581 gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat
     2641 gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa
     2701 acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataagc
     2761 ttgccacaac ccacaaggag acgaccttcc atgaaaaagc ctgaactcac cgcgacgtct
     2821 gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag
     2881 ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta
     2941 aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc
     3001 gcgctcccga ttccggaagt gcttgacatt ggggaattca gcgagagcct gacctattgc
     3061 atctcccgcc gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct
     3121 gttctgcagc cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg
     3181 agcgggttcg gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc
     3241 atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc
     3301 agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa
     3361 gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc
     3421 ataacagcgg tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc
     3481 aacatcttct tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag
     3541 cggaggcatc cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt
     3601 cttgaccaac tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag
     3661 ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc
     3721 cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac
     3781 cgacgcccca gcactcgtcc gagggcaaag gaatgaacgc gttaagtcga caatcaacct
     3841 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     3901 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     3961 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     4021 gtcaggcaac gcggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     4081 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     4141 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     4201 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     4261 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     4321 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     4381 cctcagacga gtcggatctc cctttgggcc gcctccccgc gtcgacttta agaccaatga
     4441 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     4501 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     4561 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     4621 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     4681 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtacg tatagtagtt
     4741 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     4801 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     4861 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     4921 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc
     4981 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     5041 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     5101 agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt
     5161 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     5221 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     5281 ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
     5341 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     5401 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     5461 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     5521 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     5581 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     5641 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     5701 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     5761 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     5821 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     5881 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     5941 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     6001 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     6061 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     6121 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     6181 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     6241 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     6301 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     6361 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     6421 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     6481 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     6541 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     6601 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     6661 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     6721 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     6781 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     6841 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     6901 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     6961 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     7021 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     7081 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     7141 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     7201 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     7261 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     7321 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     7381 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     7441 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     7501 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggtcttt tgctggcctt
     7561 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     7621 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     7681 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     7741 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     7801 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     7861 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     7921 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     7981 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     8041 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     8101 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
     8161 gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt
     8221 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     8281 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     8341 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     8401 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
     8461 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
     8521 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
     8581 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     8641 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
     8701 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     8761 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     8821 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     8881 accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa
     8941 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     9001 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     9061 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
     9121 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
     9181 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
     9241 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
     9301 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
     9361 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
     9421 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
     9481 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
     9541 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
     9601 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
     9661 gacccacctc ccaaccccga ggggacccag agagggccta ttttaaaaga aaagggggga
     9721 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     9781 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     9841 gagatccact ttggcgccgg ctcgag
//
