LOCUS       pLCKO-CMV-TMEM106B-M210A_F213A-P2A-Blasticidin        8183 bp    DNA     circular UNA 26-MAR-2025
DEFINITION  .
ACCESSION   urn.local...4i-iz0k6f9
VERSION     urn.local...4i-iz0k6f9
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        1..742
                     /created_by="User"
                     /label="CMV"
     source          830..1683
                     /created_by="User"
                     /label="gBlock TMEM106B PAMmut"
     CDS             846..1667
                     /created_by="u0063854"
                     /modified_by="u0063854"
                     /label="TMEM106B"
     conflict        1473..1475
                     /created_by="u0063854"
                     /label="M210A"
     conflict        1482..1484
                     /created_by="u0063854"
                     /label="F213A"
     sig_peptide     1668..1733
                     /created_by="User"
                     /modified_by="u0063854"
                     /label="P2A"
     CDS             1734..2129
                     /created_by="User"
                     /modified_by="u0063854"
                     /label="Blasticidin"
     domain          2147..2722
                     /Source="Geneious"
                     /label="WPRE"
     domain          2788..2803
                     /Source="Geneious"
                     /label="cPPT"
     domain          2805..2857
                     /Source="Geneious"
                     /label="delta_U3"
     domain          2858..3038
                     /Source="Geneious"
                     /label="HIV-1_5_LTR"
     terminator      3119..3238
                     /Source="Geneious"
                     /modified_by="u0063854"
                     /label="SV40_PA_terminator"
     domain          3256..3354
                     /Source="Geneious"
                     /label="SV40_origin"
     promoter        3433..3451
                     /Source="Geneious"
                     /modified_by="u0063854"
                     /label="T7_promoter"
     domain          3470..3618
                     /Source="Geneious"
                     /label="lacZ_a"
     domain          3636..3942
                     /Source="Geneious"
                     /label="f1_origin"
     domain          4135..4163
                     /Source="Geneious"
                     /label="AmpR_promoter"
     domain          4205..5065
                     /Source="Geneious"
                     /label="Ampicillin"
     domain          5220..5839
                     /Source="Geneious"
                     /label="pBR322_origin"
     domain          6520..6700
                     /Source="Geneious"
                     /label="HIV-1_5_LTR"
     domain          6811..6855
                     /Source="Geneious"
                     /label="HIV-1_psi_pack"
     domain          7365..7598
                     /Source="Geneious"
                     /label="RRE"
     domain          8014..8131
                     /created_by="User"
                     /modified_by="User"
                     /label="central DNA flap"
     domain          8018..8033
                     /Source="Geneious"
                     /modified_by="User"
                     /label="cPPT"
     domain          8116..8127
                     /created_by="User"
                     /label="CTS"
ORIGIN      
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tcgtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgggc ctcggcggcc aagcttggca atccggtact gttggtaaag
      841 ccaccatggg aaagtctctt tctcatttgc ctttgcattc aagcaaagaa gatgcttatg
      901 atggtgtaac gtcagagaat atgcgcaatg gactggttaa tagtgaagtc cataatgaag
      961 atggaagaaa tggagatgtc tctcagtttc catatgtgga atttacagga agagatagtg
     1021 tcacctgccc tacttgtcag ggtactggtc gtatcccacg cgggcaagaa aaccaactgg
     1081 tggcattgat tccatatagt gatcagagat taaggccaag aagaacaaag ctgtatgtga
     1141 tggcttctgt gtttgtctgt ctactccttt ctggattggc tgtgtttttc cttttccctc
     1201 ggtcaattga tgtcaagtac attggtgtaa aatcagccta tgtcagttat gatgttcaga
     1261 agcgtacaat ttatttaaat atcacaaaca cactaaatat aacaaacaat aactattact
     1321 ctgtcgaagt tgaaaacatc actgcccaag ttcaattttc gaagactgta atcggcaaag
     1381 cacgcttaaa caacataacc attattggtc cacttgatat gaaacaaatt gattacacag
     1441 tacctaccgt tatagcagag gaaatgagtt atgcgtatga tgcctgtact ctgatatcca
     1501 tcaaagtgca taacatagta ctcatgatgc aagttactgt gacaacaaca tactttggcc
     1561 actctgaaca gatatcccag gagaggtatc agtatgtcga ctgtggaaga aacacaactt
     1621 atcagttggg gcagtctgaa tatttaaatg tacttcagcc acaacagggc agcggagcaa
     1681 ccaattttag tctcctcaaa caggccggcg atgtcgaaga aaatccgggc ccagccaagc
     1741 ctttgtctca agaagaatcc accctcattg aaagagcaac ggctacaatc aacagcatcc
     1801 ccatctctga agactacagc gtcgccagcg cagctctctc tagcgacggc cgcatcttca
     1861 ctggtgtcaa tgtatatcat tttactgggg gaccttgtgc agaactcgtg gtgctgggca
     1921 ctgctgctgc tgcggcagct ggcaacctga cttgtatcgt cgcgatcgga aatgagaaca
     1981 ggggcatctt gagcccctgc ggacggtgcc gacaggtgct tctcgatctg catcctggga
     2041 tcaaagccat agtgaaggac agtgatggac agccgacggc agttgggatt cgtgaattgc
     2101 tgccctctgg ttatgtgtgg gagggctgaa cgcgttaagt cgacaatcaa cctctggatt
     2161 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     2221 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     2281 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     2341 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     2401 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     2461 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     2521 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct
     2581 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     2641 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     2701 cgagtcggat ctccctttgg gccgcctccc cgcgtcgact ttaagaccaa tgacttacaa
     2761 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     2821 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     2881 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     2941 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     3001 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt acgtatagta gttcatgtca
     3061 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt
     3121 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     3181 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     3241 tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     3301 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     3361 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg
     3421 tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac
     3481 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     3541 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     3601 gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     3661 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     3721 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     3781 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     3841 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     3901 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     3961 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     4021 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg
     4081 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     4141 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     4201 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     4261 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     4321 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     4381 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     4441 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     4501 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     4561 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     4621 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     4681 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     4741 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     4801 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     4861 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     4921 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     4981 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     5041 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     5101 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     5161 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     5221 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     5281 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     5341 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     5401 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     5461 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     5521 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     5581 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     5641 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     5701 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     5761 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     5821 gaaaaacgcc agcaacgcgg cctttttacg gttcctggtc ttttgctggc cttttgctca
     5881 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     5941 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     6001 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     6061 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     6121 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     6181 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     6241 gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct
     6301 tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa
     6361 ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta
     6421 ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc
     6481 agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag
     6541 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     6601 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     6661 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact
     6721 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc
     6781 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag
     6841 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc
     6901 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag
     6961 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag
     7021 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac
     7081 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa
     7141 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg
     7201 cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga
     7261 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
     7321 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
     7381 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
     7441 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
     7501 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc
     7561 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
     7621 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa
     7681 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
     7741 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
     7801 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
     7861 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
     7921 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
     7981 ctcccaaccc cgaggggacc cagagagggc ctattttaaa agaaaagggg ggattggggg
     8041 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
     8101 acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc
     8161 actttggcgc cggctcgagg ggg
//
