LOCUS       Exported               11938 bp ds-DNA     linear   SYN 26-MAR-2025
DEFINITION  Michmerhuizen_B2G_1_NUP98_retro_GFP     coverage: 325x.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Mar 26, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11938
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
ORIGIN
        1 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
       61 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
      121 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
      181 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
      241 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
      301 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
      361 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
      421 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
      481 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
      541 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
      601 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
      661 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
      721 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
      781 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
      841 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
      901 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
      961 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     1021 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     1081 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     1141 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     1201 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     1261 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     1321 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     1381 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     1441 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     1501 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     1561 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     1621 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     1681 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     1741 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     1801 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     1861 tgagcggata acaatttcac aattctcccc agctctttct gcgctgtcca taatgaacta
     1921 tttcaggagt attggagtat gtggattcaa aacagtggct aattgagtca ggattactta
     1981 atattcgact cctaccctca gaatgttgtt ctccaacttc aagaaactgt tacccatgaa
     2041 agacccccgc tgacgggtag tcaatcactc agaggagacc ctcccaagga acagcgagac
     2101 cacaagtcgg atgcaactgc aagagggttt attggataca cgggtacccg ggcgacgcag
     2161 tctatcggag gactggcgcg ccgagtgagg ggttgtgggc tcttttattg agctcgggga
     2221 gcagaagcgc gcgaacagaa gcgagaagcg aactgattgg ttagttcaaa taaggcacag
     2281 ggtcatttca ggtccttggg gcaccctgga aacatctgat ggttctctag aaactgctga
     2341 gggcgggacc gcatctgggg accatctgtt cttggccctg agccggggca ggaactgctt
     2401 accacagata tcctgtttgg cccatattct gctgtctctc tgttcctaac cttgatctga
     2461 acttctctat tctcagttat gtattttcca tgccttgcaa aatggcgtta cttaagctag
     2521 cttgccaaac ctacaggtgg ggtctttcat tccccccttt ttctggagac taaataaaat
     2581 cttttatttt atcgataagc ttggctgcag gtcgacggta tcgataagct tgataattct
     2641 taattaattc attacttgta cagctcgtcc atgccgagag tgatcccggc ggcggtcacg
     2701 aactccagca ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag ggcggactgg
     2761 gtgctcaggt agtggttgtc gggcagcagc acggggccgt cgccgatggg ggtgttctgc
     2821 tggtagtggt cggcgagctg cacgctgccg tcctcgatgt tgtggcggat cttgaagttc
     2881 gccttgatgc cgttcttctg cttgtcggcc atgatataga cgttgtggct gttgtagttg
     2941 tactccagct tgtgccccag gatgttgccg tcctccttga agtcgatgcc cttcagctcg
     3001 atgcggttca ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg
     3061 tcgtccttga agaagatggt gcgctcctgg acgtagcctt cgggcatggc ggacttgaag
     3121 aagtcgtgct gcttcatgtg gtcggggtag cggctgaagc actgcacgcc gtaggtcagg
     3181 gtggtcacga gggtgggcca gggcacgggc agcttgccgg tggtgcagat gaacttcagg
     3241 gtcagcttgc cgtaggtggc atcgccctcg ccctcgccag acacgctgaa cttgtggccg
     3301 tttacgtcgc cgtccagctc gaccaggatg ggcaccaccc cggtgaacag ctcctcgccc
     3361 ttgctcacca tggttgtggc catattatca tcgtgttttt caaaggaaaa ccacgtcccc
     3421 gtggttcggg gggcctagac gtttttttaa cctcgactaa acacatgtaa agcatgtgca
     3481 ccgaggcccc agatcagatc ccatacaatg gggtaccttc tgggcatcct tcagcccctt
     3541 gttgaatacg cttgaggaga gccatttgac tctttccaca actatccaac tcacaacgtg
     3601 gcactggggt tgtgccgcct ttgcaggtgt atcttataca cgtggctttt ggccgcagag
     3661 gcacctgtcg ccaggtgggg ggttccgctg cctgcaaagg gtcgctacag acgttgtttg
     3721 tcttcaagaa gcttccagag gaactgcttc cttcacgaca ttcaacagac cttgcattcc
     3781 tttggcgaga ggggaaagac ccctaggaat gctcgtcaag aagacagggc caggtttccg
     3841 ggccctcaca ttgccaaaag acggcaatat ggtggaaaat aacatataga caaacgcaca
     3901 ccggccttat tccaagcggc ttcggccagt aacgttaggg ggggggggcg gaattcgtta
     3961 tcgaaccact ttgtacaaga aagctgggtt gcggccgcac tcgagctaca ggctcccaac
     4021 agccagttct cgcagatagg actgggtaag gctgcgcagt tcgtccatgg catagtcctc
     4081 aggcatggga agccggccaa tgtggggagc caagaggcgc aaagggactc gctgagggtc
     4141 tggtgttgag tcggaggttc tatcaggagg atgatgcaga ctcagcacca cgcgcagcag
     4201 gttggctaca cgtttggcca tgtctgactg agccaggcga tctttagcac tgtaacactg
     4261 aatctgctct atccgactgc acagtgaagt cactttgatg tgtaactgct ccaggtcatt
     4321 acctgagcaa tccacctgct gtatatggcg gagcatttca atgactctaa tatagtccag
     4381 gtaaacaagc ccagatgttt cccaatcctg aattaggctg ctgcgctctg gaggtgccag
     4441 gtcttccaag aaccccttca ggtagtcata gttctcatta atgatggcat cagaagctaa
     4501 gtgtcggatg atgagcttgt ggcagcggtt ccagtgctca gccttaaata agcaaagggc
     4561 ctctaagtgc ttgtcagatt ccatgtgtgc tcgcacagct ttggcctcgt ggatccattt
     4621 ggcaggtaca cggagcttct gggtaaggaa agtctcttta gcccaagatt caggggtctc
     4681 caacagctgg cagtgccggg taagcagctc tcgaacagcc ttctcacgta tgcctgagtt
     4741 gtcaatgtgc aggaggacaa agatggccca ctcccagagc ccctcacttt caagctggcc
     4801 agcgtaactg gcctgtagca caccttcaca ctgcgctgag agatgggtgt agttaagagc
     4861 cctcagcact tcccacaagt gccagcttag gcggtagtcc aaaggatctg ctgttatgct
     4921 tcgaggctcc agcagctggt tgagatcata atgtctgtca ctgtagagtt ttagaaggtg
     4981 aaagcagaca tctcgaagtg gtgtctgtga gttttgctcc tccgctatca cacagccaga
     5041 accctccaga tacgaaggaa gtggggagca ggcatatctg tcactgtcag aggtattctg
     5101 aaatgcttct tcatacatgc tgagcgccct agaaatggag gctgttggtg gaagcaaata
     5161 ccaaagatgg atagccaggg agcgtttcca atccaactgg gagcacacgt ttatttgctt
     5221 cttttctgag agctgccaca ccggttttcc agccaacaga gcaaagatgc gcagtctctc
     5281 atcctggatg aaggagtctg cttggagctg atgccagtcc accaactgca tggtgagcag
     5341 ctcccggact gactggctac ccacaaactg agataaaaga agagcaagac gatgatcccc
     5401 tgactgctgg gccagagagc aggcctcact gatccttttg cctgtgaggt agctgaatac
     5461 agcctccaca gggctgtttt tttgggttaa ggagacttcc tcttcaatct gaggtgtggc
     5521 agtacaggat agccagcggg agaaagctct tcttcgctcc agaatttgaa tgtattcacg
     5581 gggttcattt agctggctgt caagctcctt caggtggccc catagggctt cacatagtgt
     5641 ccatgtcagg ctccagtgct tcacaatttg tgcttctggt aagtctcctg atgcttcttt
     5701 aacccaatct gcatagtcat gaatgacagc aactcccaga ttggggacaa tgagaggaca
     5761 cagttcatcc acatggacag tgctgtgttt taatttgagc tccagaggtg tctgatataa
     5821 tttcatgtct tcatctggct ttctttgtct caaactgagt ttttctaagt gaactttgaa
     5881 tggggactca gttaggggtt taacagctac tggattgggc aggaatccaa actccatgga
     5941 atcggcaatc tgatgatttt ctagttcatg agagccattc agctgttctc cactattagc
     6001 aagagtccag ttggggcccc aaccaacacg aaatgagcgt cccatgaata gggccatgtc
     6061 catcaagagt tttcccttgc cataggtgac agacttttca cgagggacta ggcctagttg
     6121 cctacgtgta cccactgttt tcaacggaac ctcaggggct gggctgggca ttgtgaacac
     6181 agaagtcagg ggtggaggga cagaccaaga ggatgtggat gggatattca ttaaagatgc
     6241 tgctctggga gtacgacatt cttgcacaga gacacttggt gaaagaaaag ctccacttgt
     6301 aaattttgat tgtagtaacc caccaactag tgaacgagtt ttcgacgagt gggatgctga
     6361 aatggggagt ctgggagaac agatttcttg agaagtatct gctttggaag gcaggcgact
     6421 gaagcgttga tccagtgcca tatctacatc ttcttcatca gtaagcaatg atgctttcat
     6481 gatctgtaag acatgtggat taattcccag tgaagatgca atatgtgttg aggcagacac
     6541 aggttcctga tcctcaggca tgctctcttc taacatggta tccaaaactg gctcccgggt
     6601 gatatctacc atgtcactgt ccagttccac aaccctccct aactgctcca cctctgggct
     6661 ctggctctga ggtggaggtg caggtttgcc attaagagcc atctgcaagg gcgtagtctg
     6721 gcttgcagga ggcaaaggag cagtcttcaa cttctttgta ctagttttag acggatgctc
     6781 ctcctcctct tcatcagaat cctgaaggcc atacttagaa aaatgggaga ccttaaacac
     6841 ccaagaacca gtttcaggcc ggtattcttt gaattgagct ccctgtttcc ttgaaactgc
     6901 ttccaatctt ccttcatagt tgatatcagc aaggcgatct gggctcttta ttaaacaacg
     6961 agatgtttta tctgttggcc aaactccatc caatgtaact tcagccttcc tatttagccc
     7021 ttcacccaca ggtggttttt ggttatcatc taagtagaca actacttctt tcctccggat
     7081 atgcacaata tcatccaaat ttagatttgt caaattcaca tctccttcaa aatagattga
     7141 accataacct ttccgaccaa tagtgaaatc agagacaatg cactctcctt tttcattggt
     7201 aattttagca aggtcatcca tagatggaat agtatagtaa ccaaccttag tgagaataat
     7261 acctgctggg tgcatatggt aagaattatt ttctatttct tctcggtcat cctgaagtga
     7321 ctcatcatga aaagacgttt cttcactgct tccttccagc ccatttcgca aagcagcacg
     7381 catgtttaat gcaacaatgg tatcatccac actgttgctg ttgctgtgtt tatttccagc
     7441 actttctggg gtttgaggaa taggtttggc aatagggtta gtataaaaat gtgaaacaag
     7501 ggaatcttca tctccatcct gctggtgatt ctcatcaaca ggtttgctta ggaaactaaa
     7561 tctctctcca ttttctggat attcagatgg tgaagctaga ttttctgaat cacgattaac
     7621 aggagaaaag agattgctat tattaaggtt cttcaaaacc aacttcttaa tgctcttctt
     7681 gggcatgaat gctccattgg ctagggatgg ttcatcgtca tccagcccat caaagagatg
     7741 tgacttggct gtgcctgttg tttgtaaagc ctttggccgg actctagtgg cagggcgggg
     7801 tgtcagttta taatgagtag gtgtagtaag agccttctgg gctgctggat ttgttggttt
     7861 caatctctct tccttcttct tagggtctga catcggattc cggaagagag gagagtctcc
     7921 aaaaggtgag tatgttagac tattgatgtg ctgctggaga acagcctgct gggcagcaga
     7981 agcatttgga tctgtcaaag ctactggggc actggggggc tccaaagccc aaagtggctg
     8041 tcgtagtatt aaatccgggg gccccaaagg ctcctgtacc aagaggccct ccaatcttag
     8101 gttggttgtt cccaaacaaa gatgcctgtc cagcaccaag agctgttcca aatcctgcac
     8161 caagcccagt tccaagagtc ccgtgtgctg gtttacttcc aaaaatacta ttcccactgg
     8221 tatttgtgcc aaaaccaaag agcccgccac tggttgtacc aaatgaaggt gcactggttg
     8281 tgctgcttcc aaatgtagta agcttgttat tgccaaacag ggtcgaacca acagcaccaa
     8341 atccagtatt ggtctgccca aagagacctg ttcctgttcc aaatgctgtc ccagtgctgg
     8401 tgtttgtagc tgtcccaaaa agacctccag gctgtgaggc ttgggttact ccaaataatc
     8461 ccatggtgtt ggtgcttggc tgtcctatgg tgctggtatt accaaaggaa aagccagtgt
     8521 tctgggtggt tgtagcctgg ccaaatggtt tgctgaagag gctggtagtc tgctgattct
     8581 gttggccaaa gagaccacct ggatttgttc caaatccagt tgtactagtt ccaaaggcag
     8641 ttttgttctg accatatgca aagcctgaat tagtggtgga ggagctgaag agtcctgttg
     8701 cgctggaagt ggctggagaa gacccaaaca agccagttgt ggtacctgct cccacctggt
     8761 tctgtgggcc cttcctgtta gcctgataat cctctaaacg aagttcctct agtgacttgc
     8821 tttcatattc tttcatagca gtaatacact ggtgcttggt acttatgtta gtgctaactc
     8881 cagctttgac catagtatct gtaccagttg gagggttaaa tttaatagta gtcccagtag
     8941 gagcagctgt aaaactactt ggcccaaaga gggagccaga tgtgctgcca aaaggattag
     9001 aggtggtatt tgtggttcca aagagtcctc cactgctagt actggttcca aaattgccaa
     9061 agccagttgg tttattttgt gcaaaggcat tgttttggga tgagaagaga ctggtccctg
     9121 tgcttgcagt tccaaacaag gtatttgctg ttcctgttga cgtaccaaac ccaaagccag
     9181 tgcttgtgga ggtagctggc tggctaaatg aactggttcc aaacaatcct cctggtttag
     9241 tctgtgaatt tccaaagagg cctccagtat tgttgctaga accaaatgca gatgttccaa
     9301 atgcccctcc actagtagtg ccaaagccag tattctgtcc aaatgttgaa gttgtgccaa
     9361 agccacctgt gccaccccca aagggtgttc caaatgattt gttaaacatg gttcgaactc
     9421 cttccaagcc tgcttttttg tacaaacttg tttgataacc tcgagagatc taattccggc
     9481 gcctagagaa ggagtgaggg ctggataaag ggaggatcga ggcggggtcg aacgaggagg
     9541 ttcaaggggg agagacgggg cggatggagg aagaggaggc ggaggcttag ggtgtacaaa
     9601 gggcttgacc cagggagggg ggtcaaaagc caaggcttcc caggtcacga tgtaggggac
     9661 ctggtctggg tgtccatgcg ggccaggtga aaagaccttg atcttaacct gggtgatgag
     9721 gtctcggtta aaggtgccgt ctcgcggcca tccgacgtta aaggttggcc attctgcaga
     9781 gcagaaggta acccaacgtc tcttcttgac atctaccgac tggttgtgag cgatccgctc
     9841 gacatctttc cagtgaccta aggtcaaact taagggagtg gtaacagtct ggccctaatt
     9901 ttcagacaaa tacagaaaca cagtcagaca gagacaacac agaacgatgc tgcagcgctg
     9961 cagcagacaa gacgcgcggc ttcggttcca aaccgaaagc aaaaattcag acggaggcgg
    10021 gaactgtttt aggttctcgt ctcctaccag aaccacatat cctgacgggg tcggattcca
    10081 catcgactcc cttcctcagg tcgggccaca aaaacggccc ccaaagtccc cgggacgtct
    10141 cccagggttg cggccgggtg ttcagaactc gtcagttcca ccacgggtcc gccagataca
    10201 gagctagtta gctaactagt acagacgcag gcgcaaacat tagatgccgg cacaaacacg
    10261 cacaaagaca gagacagaca cgaaacgacc gctggccagc ttacctcccg gcgggggggt
    10321 cggtggtccc cgggcagggg tctccaaatc tcggtggaac ctccaaatga aagacccccg
    10381 aggtgggcag tcaatcaatc tgaggagacc ctcccaagga tcagcgagtc cacgattcgg
    10441 atgcaaacag caagaggctt tattgggaat acgggtaccc gggcgacgca gtctatcgga
    10501 ggactggcgc gccgagtgag gggttgtggg ctcttttatt gagctcgggg agcagaagcg
    10561 cgcgaacaga agcgagaagc gaactgattg gttagttcaa ataaggcaca gggtcatttc
    10621 aggtccttgg ggcaccctgg aaacatctga tggttctcta gaaactgctg agggcgggac
    10681 cgcatctggg gaccatctgt tcttggccct gagccggggc aggaactgct taccacagat
    10741 atcctgtttg gcccatattc tgctgtctct ctgttcctaa ccttgatctg aacttctcta
    10801 ttctcagtta tgtattttcc atgccttgca aaatggcgtt acttaagcta gcttgccaaa
    10861 cctacaggtg gggtctttca ttcccccctt tttctggaga ctaaataaaa tcttttattt
    10921 tatctatggc tcgtactcta taggcttcag ctggtgatat tgttgagtca aaactagagc
    10981 ctggaccact gatatcctgt ctttaacaaa ttggactaat cgcctctacg ccggacgcat
    11041 cgtggccggc atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac
    11101 cgatggggaa gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat
    11161 ggtggcaggc cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct
    11221 tgcgccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
    11281 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
    11341 ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt
    11401 acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat
    11461 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
    11521 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
    11581 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta
    11641 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
    11701 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
    11761 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
    11821 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
    11881 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgag
//
