LOCUS       Exported                2158 bp ds-DNA     linear   UNA 19-NOV-2023
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Mar 26, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     promoter        11..110
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     130..158
                     /label=F.NheI.SNAPf
     CDS             144..689
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
     primer_bind     complement(706..736)
                     /label=R.XhoI.SNAPf
     primer_bind     727..754
                     
     misc_feature    735..1979
                     /label=TDP43
     primer_bind     complement(1960..1988)
                     /label=R.HindIII.TDP-43
     regulatory      2031..2040
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
ORIGIN
        1 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
       61 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct ggtttagtga
      121 accgtcagat ccgctagccc accatggaca aagactgcga aatgaagcgc accaccctgg
      181 atagccctct gggcaagctg gaactgtctg ggtgcgaaca gggcctgcac cgtatcatct
      241 tcctgggcaa aggaacatct gccgccgacg ccgtggaagt gcctgcccca gccgccgtgc
      301 tgggcggacc agagccactg atgcaggcca ccgcctggct caacgcctac tttcaccagc
      361 ctgaggccat cgaggagttc cctgtgccag ccctgcacca cccagtgttc cagcaggaga
      421 gctttacccg ccaggtgctg tggaaactgc tgaaagtggt gaagttcgga gaggtcatca
      481 gctacagcca cctggccgcc ctggccggca atcccgccgc caccgccgcc gtgaaaaccg
      541 ccctgagcgg aaatcccgtg cccattctga tcccctgcca ccgggtggtg cagggcgacc
      601 tggacgtggg gggctacgag ggcgggctcg ccgtgaaaga gtggctgctg gcccacgagg
      661 gccacagact gggcaagcct gggctgggtc ctgcaggcgg atccggtacc gaattcgcgg
      721 ccgcagctcg agctatgtct gaatatattc gggtaaccga agatgagaac gatgagccca
      781 ttgaaatacc atcggaagac gatgggacgg tgctgctctc cacggttaca gcccagtttc
      841 caggggcgtg tgggcttcgc tacaggaatc cagtgtctca gtgtatgaga ggtgtccggc
      901 tggtagaagg aattctgcat gccccagatg ctggctgggg aaatctggtg tatgttgtca
      961 actatccaaa agataacaaa agaaaaatgg atgagacaga tgcttcatca gcagtgaaag
     1021 tgaaaagagc agtccagaaa acatccgatt taatagtgtt gggtctccca tggaaaacaa
     1081 ccgaacagga cctgaaagag tattttagta cctttggaga agttcttatg gtgcaggtca
     1141 agaaagatct taagactggt cattcaaagg ggtttggctt tgttcgtttt acggaatatg
     1201 aaacacaagt gaaagtaatg tcacagcgac atatgataga tggacgatgg tgtgactgca
     1261 aacttcctaa ttctaagcaa agccaagatg agcctttgag aagcagaaaa gtgtttgtgg
     1321 ggcgctgtac agaggacatg actgaggatg agctgcggga gttcttctct cagtacgggg
     1381 atgtgatgga tgtcttcatc cccaagccat tcagggcctt tgcctttgtt acatttgcag
     1441 atgatcagat tgcgcagtct ctttgtggag aggacttgat cattaaagga atcagcgttc
     1501 atatatccaa tgccgaacct aagcacaata gcaatagaca gttagaaaga agtggaagat
     1561 ttggtggtaa tccaggtggc tttgggaatc agggtggatt tggtaatagc agagggggtg
     1621 gagctggttt gggaaacaat caaggtagta atatgggtgg tgggatgaac tttggtgcgt
     1681 tcagcattaa tccagccatg atggctgccg cccaggcagc actacagagc agttggggta
     1741 tgatgggcat gttagccagc cagcagaacc agtcaggccc atcgggtaat aaccaaaacc
     1801 aaggcaacat gcagagggag ccaaaccagg ccttcggttc tggaaataac tcttatagtg
     1861 gctctaattc tggtgcagca attggttggg gatcagcatc caatgcaggg tcgggcagtg
     1921 gttttaatgg aggctttggc tcaagcatgg attctaagtc ttctggctgg ggaatgtaga
     1981 agcttcgaat tctgcagtcg acggtaccgc gggcccggga tccaccggtc gccaccatgg
     2041 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     2101 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacgg
//
