LOCUS       Exported                7811 bp ds-DNA     circular SYN 04-OCT-2024
DEFINITION  T16.
ACCESSION   T16
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Mar 28, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     source          1..7811
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      1..589
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(747..1607)
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.5"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(1608..1712)
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             1755..1847
                     /label=tnpA (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=96.8"
                     /note="/match_length=6.5"
                     /note="/other=CDS"
     polyA_signal    complement(1868..1916)
                     /label=poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     CDS             complement(1980..2772)
                     /label=NeoR/KanR (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.7"
                     /note="/match_length=99.7"
                     /note="/other=CDS"
     CDS             complement(2823..3755)
                     /label=hRluc
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=99.7"
                     /note="/other=CDS"
     promoter        complement(3806..4163)
                     /label=SV40 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    4228..4279
                     /label=bom (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=36.9"
                     /note="/other=misc_feature"
     rep_origin      complement(4280..4312)
                     /direction=LEFT
                     /label=M13 ori (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.0"
                     /note="/match_length=7.3"
                     /note="/other=rep_origin"
     CDS             4317..4385
                     /label=large T antigen (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.1"
                     /note="/match_length=3.2"
                     /note="/other=CDS"
     polyA_signal    4408..4529
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     terminator      complement(4551..4570)
                     /label=T7 terminator (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=41.7"
                     /note="/other=terminator"
     CDS             complement(4578..4627)
                     /label=SET1 (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=39.7"
                     /note="/other=CDS"
     misc_feature    complement(4634..4658)
                     /label=MCS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=43.9"
                     /note="/other=misc_feature"
     CDS             complement(5158..6810)
                     /label=luciferase
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(6857..7356)
                     /label=PGK promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    complement(7413..7696)
                     /label=cer region
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
ORIGIN
        1 ttgaaatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccggttg
      721 ggcgtcgctt ggtcggtcat ttcgaattac caatgcttaa tcagtgaggc acctatctca
      781 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
      841 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
      901 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
      961 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     1021 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     1081 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     1141 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat agttgtcaga
     1201 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     1261 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     1321 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     1381 ccacatagca gaactttgaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     1441 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     1501 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     1561 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     1621 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     1681 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     1741 gtctaagaaa ccattacgta gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat
     1801 gtcagctact gggctatctg gacaagggaa aacgcaagcg caaagaggat ccttatcgct
     1861 atcgattcac acaaaaaacc aacacacaga tgtaatgaaa ataaagatat tttattgcgg
     1921 ccatcgtgat ggcaggttgg gcgtcgcttg gtcggtcatt tcgaacccca gagtcccgct
     1981 cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata
     2041 ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aatatcacgg
     2101 gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca gtcgatgaat
     2161 ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc atgggtcacg
     2221 acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg
     2281 agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc catccgagta
     2341 cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc
     2401 gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg agcaaggtga
     2461 gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct tcccgcttca
     2521 gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca cgatagccgc
     2581 gctgcctcgt cctgcagttc attcagggca ccggacaggt cggtcttgac aaaaagaacc
     2641 gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat tgtctgttgt
     2701 gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc gtgcaatcca
     2761 tcttgttcaa tccctccgga gcctccgcca cctgatccgc cacctccgct cccaccaccg
     2821 gtctgctcgt tcttcagcac gcgctccacg aagctcttga tgtacttacc catttcatct
     2881 ggagcgtcct cctggctgaa gtggaggccc ttcaccttca cgaactcggt gttagggaac
     2941 ttcttagctc cctcgacaat agcgttggaa aagaacccag ggtcggactc gatgaacatc
     3001 ttaggcagat cgtcgctggc ccgaaggtag gcgttgtagt tgcggacaat ctggacgacg
     3061 tcgggcttgc ctcccttaac gagagggatc tcgcgaggcc aggagagggt aggccgtcta
     3121 acctcgccct tctccttgaa tggctccagg taggcagcga actcctcagg ctccagtttc
     3181 cgcatgatct tgcttgggag catggtctcg acgaagaagt tattctcaag caccattttc
     3241 tcgccctctt cgctcttgat cagggcgata tcctcctcga tgtcaggcca ctcgtcccag
     3301 gactcgatca cgtccacgac actctcagca tggacgatgg ccttgatctt gtcttggtgc
     3361 tcgtaggagt agtgaaaggc cagacaagcc ccccagtcgt ggcccacaaa gatgattttc
     3421 tttggaaggt tcagcagctc gaaccaagcg gtgaggtact tgtagtgatc caggaggcga
     3481 tatgagccat tcccgctctt gccggactta cccattccga tcagatcagg gatgatgcat
     3541 ctagccacgg gctcgatgtg aggcacgacg tgcctccaca ggtagctgga ggcagcgtta
     3601 ccatgcagaa aaatcacggc gttctcggcg tgcttctcgg aatcatagta gttgatgaag
     3661 gagtccagca cgttcatttg cttgcagcga gcccaccact gaggcccagt gatcatgcgt
     3721 ttgcgttgct cggggtcgta caccttggaa gccatggtgg ctccagcctt tggttcgaga
     3781 ctgttgtgtc agaagaatca agctttttgc aaaagcctag gcctccaaaa aagcctcctc
     3841 actacttctg gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa
     3901 ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg
     3961 gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc
     4021 ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt
     4081 gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc
     4141 acagctggtt ctttccgcct cagaaggtac ctaaccaagt tcctctttca gaggttattt
     4201 caggccatgg tgctgcgcag atccgcgtat gcggtgtgaa ataccgcaca gatgcgtaag
     4261 gagaaaatac cgcatcagga aattgtaagc gttaatattt tgttaaaatt cgattttacc
     4321 acatttgtag aggttttact tgcttaaaaa aacctcccac atctccccct gaacctgaaa
     4381 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     4441 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     4501 ggtttgtcca aactcatcaa tgtatcttat catgtctgct cgaagcggcc gccccaaggg
     4561 gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt cgggctttgt
     4621 tagcagccgg atcagcttgc atgcctgcag gtcgactccc agctttgcta tttatttaaa
     4681 tgtcattctc caacacatat ggggaatgac atatgcttcc cattattaca tggcaaaaca
     4741 tactccatat ccagaaaaaa tatttatatg acatatggcc tagtattcta aatcaaggca
     4801 ggatcttcca acaagagcag gagagagaag aaaacattta cattctataa catgattata
     4861 agaattgaga tagatgaaat gaaaaacaac atctttgaac aattacagtt gacagaacag
     4921 ctttccctta ttctacttgc tttaaataac tcatatctgt aaacttgaac accaactctg
     4981 gctgatttaa cagtcttaat cccttgattt tccatactga acatcaccca atatatagtg
     5041 aagatcaaag gcgacttctt ccaccaagct ctgtgtccag ctgtgtttct gaggtcatac
     5101 gtgtcccctc acggacacca gaactcgagg ctagcgagct cgtttaaaca actagaatta
     5161 cacggcgatc ttgccgccct tcttggcctt aatgagaatc tcgcggatct tgcgggcgtc
     5221 caacttgccg gtcagtcctt taggcacctc gtccacgaac acaacaccac cgcgcagctt
     5281 cttggcggtt gtaacctggc tggccacata gtccacgatc tccttctcgg tcatggtttt
     5341 accgtgttcc agcacgacga ctgcggcggg cagctcgccg gcatcgtcgt cgggcaggcc
     5401 ggcgaccccg gcgtcgaaga tgttggggtg ttgcagcagg atgctctcca gttcggctgg
     5461 ggctacctgg tagcccttgt atttgatcag gctcttcagc cggtccacga tgaagaagtg
     5521 ctcgtcctcg tcccagtagg cgatgtcgcc gctgtgcagc cagccgtcct tgtcgatgag
     5581 agcgtttgta gcctcggggt tgttaacgta gccgctcatg atcatggggc cacggacgca
     5641 cagctcgccg cgctggttca cacccagtgt cttaccggtg tccaagtcca ccaccttagc
     5701 ctcgaagaag ggcaccacct tgcctactgc gccaggcttg tcgtcccctt cgggggtgat
     5761 cagaatggcg ctggttgttt ctgtcaggcc gtagccctgg cggatgcctg gtaggtggaa
     5821 gcgtttggcc acggcctcac ctacctcctt gctgagcggc gccccgccgc tggcgatctc
     5881 gtgcaagttg cttaggtcgt acttgtcgat gagagtgctc ttagcgaaga agctaaatag
     5941 tgtgggcacc agcagggcag attgaatctt atagtcttgc aagctgcgca agaatagctc
     6001 ctcctcgaag cggtacatga gcacgacccg aaagccgcag atcaagtagc ccagcgtggt
     6061 gaacatgccg aagccgtggt gaaatggcac cacgctgagg atagcggtgt cggggatgat
     6121 ctggttgccg aagatggggt cgcgggcatg actgaatcgg acacaagcgg tgcggtgcgg
     6181 tagggctacg cccttgggca atccggtact gccactactg ttcatgatca gggcgatggt
     6241 tttgtcccgg tcgaagctct cgggcacgaa gtcgtactcg ttgaagccgg gtggcaaatg
     6301 ggaagtcacg aaggtgtaca tgctttggaa gccctggtag tcggtcttgc tatccatgat
     6361 gatgatcttt tgtatgatcg gtagcttctt ttgcacgttg aggatctttt gcagcccttt
     6421 cttgctcacg aatacgacgg tgggctggct gatgcccatg ctgttcagca gctcgcgctc
     6481 gttgtagatg tcgttagctg gggccacagc cacaccgatg aacagggcac ccaacacggg
     6541 catgaagaac tgcaagctat tctcgctgca caccacgatc cgatggtttg tattcagccc
     6601 atagcgcttc atagcttctg ccagccgaac gctcatctcg aagtactcgg cgtaggtaat
     6661 gtccacctcg atatgtgcgt cggtaaaggc gatggtgccg ggcaccaggg cgtagcgctt
     6721 catggctttg tgcagctgct cgccggcggt cccgtcttcg agtgggtaga atggcgctgg
     6781 gcccttctta atgtttttgg catcttccat ggtggcttta ccaacagtac cggattgcca
     6841 agcttgggct gcaggtcgaa aggcccggag atgaggaaga ggagaacagc gcggcagacg
     6901 tgcgcttttg aagcgtgcag aatgccgggc ctccggagga ccttcgggcg cccgccccgc
     6961 ccctgagccc gcccctgagc ccgcccccgg acccacccct tcccagcctc tgagcccaga
     7021 aagcgaagga gcaaagctgc tattggccgc tgccccaaag gcctacccgc ttccattgct
     7081 cagcggtgct gtccatctgc acgagactag tgagacgtgc tacttccatt tgtcacgtcc
     7141 tgcacgacgc gagctgcggg gcggggggga acttcctgac taggggagga gtagaaggtg
     7201 gcgcgaaggg gccaccaaag aacggagccg gttggcgcct accggtggat gtggaatgtg
     7261 tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag cggggctgct aaagcgcatg
     7321 ctccagactg ccttgggaaa agcgcctccc ctacccggta gatctcgatc ctctacgccg
     7381 gacgcatcgt ggccggagta ctgcggttta tctcgatggc tacgagggca gacagtaagt
     7441 ggatttacca taatccctta attgtacgca ccgctaaaac gcgttcagcg cgatcacggc
     7501 agcagacagg taaaaatggc aacaaaccac ccgaaaaact gccgcaatcg cgcctgataa
     7561 attttaaccg tatgaatacc tatgcaacca gagggtacag gccacattac ccccacttaa
     7621 tccactgaag ctgccatttt tcatggtttc accatcccag cgaagggcca tccagcgtgc
     7681 gttcctgtat ttccggacta taaaaggatc taggtgaaga tcctttttga taatctcatg
     7741 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     7801 aaaggatctt c
//
