LOCUS       Exported                8330 bp ds-DNA     circular SYN 04-OCT-2024
DEFINITION  15_1.
ACCESSION   15_1
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8330)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Mar 28, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     source          1..8330
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      1..589
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(747..1607)
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.5"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(1608..1712)
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             1755..1847
                     /label=tnpA (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=96.8"
                     /note="/match_length=6.5"
                     /note="/other=CDS"
     polyA_signal    complement(1868..1916)
                     /label=poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     CDS             complement(1980..2772)
                     /label=NeoR/KanR (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.7"
                     /note="/match_length=99.7"
                     /note="/other=CDS"
     CDS             complement(2823..3755)
                     /label=hRluc
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=99.7"
                     /note="/other=CDS"
     promoter        complement(3806..4163)
                     /label=SV40 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    4228..4279
                     /label=bom (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=36.9"
                     /note="/other=misc_feature"
     rep_origin      complement(4280..4312)
                     /direction=LEFT
                     /label=M13 ori (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.0"
                     /note="/match_length=7.3"
                     /note="/other=rep_origin"
     CDS             4317..4385
                     /label=large T antigen (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.1"
                     /note="/match_length=3.2"
                     /note="/other=CDS"
     polyA_signal    4408..4529
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     terminator      complement(4551..4570)
                     /label=T7 terminator (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=41.7"
                     /note="/other=terminator"
     CDS             complement(4578..4627)
                     /label=SET1 (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=39.7"
                     /note="/other=CDS"
     misc_feature    complement(4634..4657)
                     /label=MCS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=42.1"
                     /note="/other=misc_feature"
     promoter        4717..4738
                     /label=ADH1 promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=95.5"
                     /note="/match_length=5.5"
                     /note="/other=promoter"
     CDS             4740..4817
                     /label=NEK5 (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=84.6"
                     /note="/match_length=3.7"
                     /note="/other=CDS"
     CDS             4824..4958
                     /label=C9orf85 (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=68.9"
                     /note="/match_length=25.1"
                     /note="/other=CDS"
     CDS             5041..5196
                     /label=PPP5D1 (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=57.7"
                     /note="/match_length=30.4"
                     /note="/other=CDS"
     CDS             complement(5677..7329)
                     /label=luciferase
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(7376..7875)
                     /label=PGK promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    complement(7932..8215)
                     /label=cer region
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
ORIGIN
        1 ttgaaatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccggttg
      721 ggcgtcgctt ggtcggtcat ttcgaattac caatgcttaa tcagtgaggc acctatctca
      781 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
      841 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
      901 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
      961 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     1021 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     1081 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     1141 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat agttgtcaga
     1201 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     1261 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     1321 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     1381 ccacatagca gaactttgaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     1441 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     1501 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     1561 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     1621 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     1681 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     1741 gtctaagaaa ccattacgta gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat
     1801 gtcagctact gggctatctg gacaagggaa aacgcaagcg caaagaggat ccttatcgct
     1861 atcgattcac acaaaaaacc aacacacaga tgtaatgaaa ataaagatat tttattgcgg
     1921 ccatcgtgat ggcaggttgg gcgtcgcttg gtcggtcatt tcgaacccca gagtcccgct
     1981 cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata
     2041 ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aatatcacgg
     2101 gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca gtcgatgaat
     2161 ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc atgggtcacg
     2221 acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg
     2281 agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc catccgagta
     2341 cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc
     2401 gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg agcaaggtga
     2461 gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct tcccgcttca
     2521 gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca cgatagccgc
     2581 gctgcctcgt cctgcagttc attcagggca ccggacaggt cggtcttgac aaaaagaacc
     2641 gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat tgtctgttgt
     2701 gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc gtgcaatcca
     2761 tcttgttcaa tccctccgga gcctccgcca cctgatccgc cacctccgct cccaccaccg
     2821 gtctgctcgt tcttcagcac gcgctccacg aagctcttga tgtacttacc catttcatct
     2881 ggagcgtcct cctggctgaa gtggaggccc ttcaccttca cgaactcggt gttagggaac
     2941 ttcttagctc cctcgacaat agcgttggaa aagaacccag ggtcggactc gatgaacatc
     3001 ttaggcagat cgtcgctggc ccgaaggtag gcgttgtagt tgcggacaat ctggacgacg
     3061 tcgggcttgc ctcccttaac gagagggatc tcgcgaggcc aggagagggt aggccgtcta
     3121 acctcgccct tctccttgaa tggctccagg taggcagcga actcctcagg ctccagtttc
     3181 cgcatgatct tgcttgggag catggtctcg acgaagaagt tattctcaag caccattttc
     3241 tcgccctctt cgctcttgat cagggcgata tcctcctcga tgtcaggcca ctcgtcccag
     3301 gactcgatca cgtccacgac actctcagca tggacgatgg ccttgatctt gtcttggtgc
     3361 tcgtaggagt agtgaaaggc cagacaagcc ccccagtcgt ggcccacaaa gatgattttc
     3421 tttggaaggt tcagcagctc gaaccaagcg gtgaggtact tgtagtgatc caggaggcga
     3481 tatgagccat tcccgctctt gccggactta cccattccga tcagatcagg gatgatgcat
     3541 ctagccacgg gctcgatgtg aggcacgacg tgcctccaca ggtagctgga ggcagcgtta
     3601 ccatgcagaa aaatcacggc gttctcggcg tgcttctcgg aatcatagta gttgatgaag
     3661 gagtccagca cgttcatttg cttgcagcga gcccaccact gaggcccagt gatcatgcgt
     3721 ttgcgttgct cggggtcgta caccttggaa gccatggtgg ctccagcctt tggttcgaga
     3781 ctgttgtgtc agaagaatca agctttttgc aaaagcctag gcctccaaaa aagcctcctc
     3841 actacttctg gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa
     3901 ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg
     3961 gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc
     4021 ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt
     4081 gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc
     4141 acagctggtt ctttccgcct cagaaggtac ctaaccaagt tcctctttca gaggttattt
     4201 caggccatgg tgctgcgcag atccgcgtat gcggtgtgaa ataccgcaca gatgcgtaag
     4261 gagaaaatac cgcatcagga aattgtaagc gttaatattt tgttaaaatt cgattttacc
     4321 acatttgtag aggttttact tgcttaaaaa aacctcccac atctccccct gaacctgaaa
     4381 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     4441 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     4501 ggtttgtcca aactcatcaa tgtatcttat catgtctgct cgaagcggcc gccccaaggg
     4561 gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt cgggctttgt
     4621 tagcagccgg atcagcttgc atgcctgcag gtcgactttt catttcagca tcttgttatt
     4681 tatttttcat tattttaaaa aattatcatt attattttct tttctttttt tttttttttg
     4741 agatggagtt tcactcttgt catccaggct ggagtgcaat ggcacgatct cggctcactg
     4801 caacctccgc ctcccaggtt caagcgaatt ctcttacctc agcctcccta gtagctagga
     4861 ttacaggcat gcaccaccaa acccaacaca tttttgtatt tttagtagag atgaggtttc
     4921 accatgttgg ccaggctggt ctcaaactcc tgacctcagg tgatccaccc acctcagcct
     4981 cccaaagtgt tgggattaca ggcatgagcc actgtgacca gcctattatt attttcaaga
     5041 cagggtctca ctctgttgcc cagcctggag tgcagtggtg tgatcatagc tcaccgcagc
     5101 ctcaatctcc tgggctgaag tgatccttct gtctcagcct ccccaacagc tgggagtata
     5161 gcggtgcaac accacaccca gctttgctat ttatttaaat gtcattctcc aacacatatg
     5221 gggaatgaca tatgcttccc attattacat agcaaaacat actccatatc cagaaaaaat
     5281 atttatatga catatggcct agtattctaa atcaaggcag gatcttccaa caagagcagg
     5341 agagagaaga aaacatttac attctataac atgattataa gaattgagat agatgaaatg
     5401 aaaaacaaca tctttgaaca attacagttg acagaacagc tttcccttat tctacttgct
     5461 ttaaataact catatctgta aacttgaaca ccaactctgg ctgatttaac agtcttaatc
     5521 ccttgatttt ccatactgaa catcacccaa tatatagtga agatcaaagg cgacttcttc
     5581 caccaagctc tgtgtccagc tgtgtttctg aggtcatacg tgtcccctca cggacaccag
     5641 aactcgaggc tagcgagctc gtttaaacaa ctagaattac acggcgatct tgccgccctt
     5701 cttggcctta atgagaatct cgcggatctt gcgggcgtcc aacttgccgg tcagtccttt
     5761 aggcacctcg tccacgaaca caacaccacc gcgcagcttc ttggcggttg taacctggct
     5821 ggccacatag tccacgatct ccttctcggt catggtttta ccgtgttcca gcacgacgac
     5881 tgcggcgggc agctcgccgg catcgtcgtc gggcaggccg gcgaccccgg cgtcgaagat
     5941 gttggggtgt tgcagcagga tgctctccag ttcggctggg gctacctggt agcccttgta
     6001 tttgatcagg ctcttcagcc ggtccacgat gaagaagtgc tcgtcctcgt cccagtaggc
     6061 gatgtcgccg ctgtgcagcc agccgtcctt gtcgatgaga gcgtttgtag cctcggggtt
     6121 gttaacgtag ccgctcatga tcatggggcc acggacgcac agctcgccgc gctggttcac
     6181 acccagtgtc ttaccggtgt ccaagtccac caccttagcc tcgaagaagg gcaccacctt
     6241 gcctactgcg ccaggcttgt cgtccccttc gggggtgatc agaatggcgc tggttgtttc
     6301 tgtcaggccg tagccctggc ggatgcctgg taggtggaag cgtttggcca cggcctcacc
     6361 tacctccttg ctgagcggcg ccccgccgct ggcgatctcg tgcaagttgc ttaggtcgta
     6421 cttgtcgatg agagtgctct tagcgaagaa gctaaatagt gtgggcacca gcagggcaga
     6481 ttgaatctta tagtcttgca agctgcgcaa gaatagctcc tcctcgaagc ggtacatgag
     6541 cacgacccga aagccgcaga tcaagtagcc cagcgtggtg aacatgccga agccgtggtg
     6601 aaatggcacc acgctgagga tagcggtgtc ggggatgatc tggttgccga agatggggtc
     6661 gcgggcatga ctgaatcgga cacaagcggt gcggtgcggt agggctacgc ccttgggcaa
     6721 tccggtactg ccactactgt tcatgatcag ggcgatggtt ttgtcccggt cgaagctctc
     6781 gggcacgaag tcgtactcgt tgaagccggg tggcaaatgg gaagtcacga aggtgtacat
     6841 gctttggaag ccctggtagt cggtcttgct atccatgatg atgatctttt gtatgatcgg
     6901 tagcttcttt tgcacgttga ggatcttttg cagccctttc ttgctcacga atacgacggt
     6961 gggctggctg atgcccatgc tgttcagcag ctcgcgctcg ttgtagatgt cgttagctgg
     7021 ggccacagcc acaccgatga acagggcacc caacacgggc atgaagaact gcaagctatt
     7081 ctcgctgcac accacgatcc gatggtttgt attcagccca tagcgcttca tagcttctgc
     7141 cagccgaacg ctcatctcga agtactcggc gtaggtaatg tccacctcga tatgtgcgtc
     7201 ggtaaaggcg atggtgccgg gcaccagggc gtagcgcttc atggctttgt gcagctgctc
     7261 gccggcggtc ccgtcttcga gtgggtagaa tggcgctggg cccttcttaa tgtttttggc
     7321 atcttccatg gtggctttac caacagtacc ggattgccaa gcttgggctg caggtcgaaa
     7381 ggcccggaga tgaggaagag gagaacagcg cggcagacgt gcgcttttga agcgtgcaga
     7441 atgccgggcc tccggaggac cttcgggcgc ccgccccgcc cctgagcccg cccctgagcc
     7501 cgcccccgga cccacccctt cccagcctct gagcccagaa agcgaaggag caaagctgct
     7561 attggccgct gccccaaagg cctacccgct tccattgctc agcggtgctg tccatctgca
     7621 cgagactagt gagacgtgct acttccattt gtcacgtcct gcacgacgcg agctgcgggg
     7681 cgggggggaa cttcctgact aggggaggag tagaaggtgg cgcgaagggg ccaccaaaga
     7741 acggagccgg ttggcgccta ccggtggatg tggaatgtgt gcgaggccag aggccacttg
     7801 tgtagcgcca agtgcccagc ggggctgcta aagcgcatgc tccagactgc cttgggaaaa
     7861 gcgcctcccc tacccggtag atctcgatcc tctacgccgg acgcatcgtg gccggagtac
     7921 tgcggtttat ctcgatggct acgagggcag acagtaagtg gatttaccat aatcccttaa
     7981 ttgtacgcac cgctaaaacg cgttcagcgc gatcacggca gcagacaggt aaaaatggca
     8041 acaaaccacc cgaaaaactg ccgcaatcgc gcctgataaa ttttaaccgt atgaatacct
     8101 atgcaaccag agggtacagg ccacattacc cccacttaat ccactgaagc tgccattttt
     8161 catggtttca ccatcccagc gaagggccat ccagcgtgcg ttcctgtatt tccggactat
     8221 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     8281 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
//
