LOCUS       Exported                8521 bp ds-DNA     circular SYN 04-OCT-2024
DEFINITION  T18.
ACCESSION   T18
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8521)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Mar 28, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     source          1..8521
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      1..589
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(747..1607)
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.5"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(1608..1712)
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             1755..1847
                     /label=tnpA (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=96.8"
                     /note="/match_length=6.5"
                     /note="/other=CDS"
     polyA_signal    complement(1868..1916)
                     /label=poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     CDS             complement(1980..2772)
                     /label=NeoR/KanR (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.7"
                     /note="/match_length=99.7"
                     /note="/other=CDS"
     CDS             complement(2823..3755)
                     /label=hRluc
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=99.7"
                     /note="/other=CDS"
     promoter        complement(3806..4163)
                     /label=SV40 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    4228..4279
                     /label=bom (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=36.9"
                     /note="/other=misc_feature"
     rep_origin      complement(4280..4312)
                     /direction=LEFT
                     /label=M13 ori (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.0"
                     /note="/match_length=7.3"
                     /note="/other=rep_origin"
     CDS             4317..4385
                     /label=large T antigen (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.1"
                     /note="/match_length=3.2"
                     /note="/other=CDS"
     polyA_signal    4408..4529
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     terminator      complement(4551..4570)
                     /label=T7 terminator (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=41.7"
                     /note="/other=terminator"
     CDS             complement(4578..4627)
                     /label=SET1 (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=39.7"
                     /note="/other=CDS"
     misc_feature    complement(4634..4657)
                     /label=MCS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=42.1"
                     /note="/other=misc_feature"
     CDS             complement(5868..7520)
                     /label=luciferase
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(7567..8066)
                     /label=PGK promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    complement(8123..8406)
                     /label=cer region
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
ORIGIN
        1 ttgaaatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccggttg
      721 ggcgtcgctt ggtcggtcat ttcgaattac caatgcttaa tcagtgaggc acctatctca
      781 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
      841 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
      901 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
      961 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     1021 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     1081 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     1141 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat agttgtcaga
     1201 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     1261 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     1321 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     1381 ccacatagca gaactttgaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     1441 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     1501 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     1561 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     1621 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     1681 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     1741 gtctaagaaa ccattacgta gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat
     1801 gtcagctact gggctatctg gacaagggaa aacgcaagcg caaagaggat ccttatcgct
     1861 atcgattcac acaaaaaacc aacacacaga tgtaatgaaa ataaagatat tttattgcgg
     1921 ccatcgtgat ggcaggttgg gcgtcgcttg gtcggtcatt tcgaacccca gagtcccgct
     1981 cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata
     2041 ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aatatcacgg
     2101 gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca gtcgatgaat
     2161 ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc atgggtcacg
     2221 acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg
     2281 agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc catccgagta
     2341 cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc
     2401 gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg agcaaggtga
     2461 gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct tcccgcttca
     2521 gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca cgatagccgc
     2581 gctgcctcgt cctgcagttc attcagggca ccggacaggt cggtcttgac aaaaagaacc
     2641 gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat tgtctgttgt
     2701 gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc gtgcaatcca
     2761 tcttgttcaa tccctccgga gcctccgcca cctgatccgc cacctccgct cccaccaccg
     2821 gtctgctcgt tcttcagcac gcgctccacg aagctcttga tgtacttacc catttcatct
     2881 ggagcgtcct cctggctgaa gtggaggccc ttcaccttca cgaactcggt gttagggaac
     2941 ttcttagctc cctcgacaat agcgttggaa aagaacccag ggtcggactc gatgaacatc
     3001 ttaggcagat cgtcgctggc ccgaaggtag gcgttgtagt tgcggacaat ctggacgacg
     3061 tcgggcttgc ctcccttaac gagagggatc tcgcgaggcc aggagagggt aggccgtcta
     3121 acctcgccct tctccttgaa tggctccagg taggcagcga actcctcagg ctccagtttc
     3181 cgcatgatct tgcttgggag catggtctcg acgaagaagt tattctcaag caccattttc
     3241 tcgccctctt cgctcttgat cagggcgata tcctcctcga tgtcaggcca ctcgtcccag
     3301 gactcgatca cgtccacgac actctcagca tggacgatgg ccttgatctt gtcttggtgc
     3361 tcgtaggagt agtgaaaggc cagacaagcc ccccagtcgt ggcccacaaa gatgattttc
     3421 tttggaaggt tcagcagctc gaaccaagcg gtgaggtact tgtagtgatc caggaggcga
     3481 tatgagccat tcccgctctt gccggactta cccattccga tcagatcagg gatgatgcat
     3541 ctagccacgg gctcgatgtg aggcacgacg tgcctccaca ggtagctgga ggcagcgtta
     3601 ccatgcagaa aaatcacggc gttctcggcg tgcttctcgg aatcatagta gttgatgaag
     3661 gagtccagca cgttcatttg cttgcagcga gcccaccact gaggcccagt gatcatgcgt
     3721 ttgcgttgct cggggtcgta caccttggaa gccatggtgg ctccagcctt tggttcgaga
     3781 ctgttgtgtc agaagaatca agctttttgc aaaagcctag gcctccaaaa aagcctcctc
     3841 actacttctg gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa
     3901 ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg
     3961 gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc
     4021 ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt
     4081 gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc
     4141 acagctggtt ctttccgcct cagaaggtac ctaaccaagt tcctctttca gaggttattt
     4201 caggccatgg tgctgcgcag atccgcgtat gcggtgtgaa ataccgcaca gatgcgtaag
     4261 gagaaaatac cgcatcagga aattgtaagc gttaatattt tgttaaaatt cgattttacc
     4321 acatttgtag aggttttact tgcttaaaaa aacctcccac atctccccct gaacctgaaa
     4381 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     4441 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     4501 ggtttgtcca aactcatcaa tgtatcttat catgtctgct cgaagcggcc gccccaaggg
     4561 gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt cgggctttgt
     4621 tagcagccgg atcagcttgc atgcctgcag gtcgacttag gtttgttaac attaacacat
     4681 gctttattca agagataata ttcaaagagt taaatcctaa gagttatcca ccctacagta
     4741 aaaaggggaa gtgggtacca cttatgacat gtacataaat tccactttta tatttctgaa
     4801 taaagctgca attgcttctt tgatagagcc atttcttaaa acttttgcta ataaggctat
     4861 gtgaactgtg ttcagaaact ttgacaacat gcacactcac tcctctcaaa gtcagtaccc
     4921 aggattttca ctccaggctg agtacctatt aagtaactag gacttcagac tgcatgttac
     4981 tatatgaatt caatttgact cacctccagt atgtttatct accacaacta ttgtttaaaa
     5041 taatcagatg aatgtttatc ataactttat aactccccca aattatactt cagtatttaa
     5101 catggtagtt tcaaaaaata aatattcaag gccccagttt taaattttcc tccaatgtaa
     5161 tccacaaagt tgataataca tgttaggagc actactctaa aataaatgta gctttctttt
     5221 aaaattactt gttgcaatag agatcacatt ctattccaaa ttctgataag gaatctgttt
     5281 ttttggtaat acaacaaaaa tttgggatga gtcagtcctt ggaaaatctt aatagtagtc
     5341 actgaacgaa gtcaatcatt ccgagagcca caaacactca aaaattactg ctagcattga
     5401 caagttcttg tgttttttga tggttattgc tttagttggc aatatctgaa tacaagacaa
     5461 aagaacatct cccattaagc cttatgttaa atattacaaa acttatacag aagatagact
     5521 catagaggct ataaagctga tgtcttaagt aactcaccca tgaaatgggt gcaggttttt
     5581 taaaggctat ccaataatct ttttactcta aaaataaatg cccctacttt tcagtgttcc
     5641 cattcttcaa caggagactc gagcagctta acagttttgc tataaaaaca aaccattctt
     5701 cttaacaaac caagaatata aaaggaaacc caataaagga cgtacttttc acagttaaca
     5761 ataaataaaa gtgcaataag tgttcaagta gggcatgtag tataaaaact acacatgagt
     5821 atttagcata ggactcgagg ctagcgagct cgtttaaaca actagaatta cacggcgatc
     5881 ttgccgccct tcttggcctt aatgagaatc tcgcggatct tgcgggcgtc caacttgccg
     5941 gtcagtcctt taggcacctc gtccacgaac acaacaccac cgcgcagctt cttggcggtt
     6001 gtaacctggc tggccacata gtccacgatc tccttctcgg tcatggtttt accgtgttcc
     6061 agcacgacga ctgcggcggg cagctcgccg gcatcgtcgt cgggcaggcc ggcgaccccg
     6121 gcgtcgaaga tgttggggtg ttgcagcagg atgctctcca gttcggctgg ggctacctgg
     6181 tagcccttgt atttgatcag gctcttcagc cggtccacga tgaagaagtg ctcgtcctcg
     6241 tcccagtagg cgatgtcgcc gctgtgcagc cagccgtcct tgtcgatgag agcgtttgta
     6301 gcctcggggt tgttaacgta gccgctcatg atcatggggc cacggacgca cagctcgccg
     6361 cgctggttca cacccagtgt cttaccggtg tccaagtcca ccaccttagc ctcgaagaag
     6421 ggcaccacct tgcctactgc gccaggcttg tcgtcccctt cgggggtgat cagaatggcg
     6481 ctggttgttt ctgtcaggcc gtagccctgg cggatgcctg gtaggtggaa gcgtttggcc
     6541 acggcctcac ctacctcctt gctgagcggc gccccgccgc tggcgatctc gtgcaagttg
     6601 cttaggtcgt acttgtcgat gagagtgctc ttagcgaaga agctaaatag tgtgggcacc
     6661 agcagggcag attgaatctt atagtcttgc aagctgcgca agaatagctc ctcctcgaag
     6721 cggtacatga gcacgacccg aaagccgcag atcaagtagc ccagcgtggt gaacatgccg
     6781 aagccgtggt gaaatggcac cacgctgagg atagcggtgt cggggatgat ctggttgccg
     6841 aagatggggt cgcgggcatg actgaatcgg acacaagcgg tgcggtgcgg tagggctacg
     6901 cccttgggca atccggtact gccactactg ttcatgatca gggcgatggt tttgtcccgg
     6961 tcgaagctct cgggcacgaa gtcgtactcg ttgaagccgg gtggcaaatg ggaagtcacg
     7021 aaggtgtaca tgctttggaa gccctggtag tcggtcttgc tatccatgat gatgatcttt
     7081 tgtatgatcg gtagcttctt ttgcacgttg aggatctttt gcagcccttt cttgctcacg
     7141 aatacgacgg tgggctggct gatgcccatg ctgttcagca gctcgcgctc gttgtagatg
     7201 tcgttagctg gggccacagc cacaccgatg aacagggcac ccaacacggg catgaagaac
     7261 tgcaagctat tctcgctgca caccacgatc cgatggtttg tattcagccc atagcgcttc
     7321 atagcttctg ccagccgaac gctcatctcg aagtactcgg cgtaggtaat gtccacctcg
     7381 atatgtgcgt cggtaaaggc gatggtgccg ggcaccaggg cgtagcgctt catggctttg
     7441 tgcagctgct cgccggcggt cccgtcttcg agtgggtaga atggcgctgg gcccttctta
     7501 atgtttttgg catcttccat ggtggcttta ccaacagtac cggattgcca agcttgggct
     7561 gcaggtcgaa aggcccggag atgaggaaga ggagaacagc gcggcagacg tgcgcttttg
     7621 aagcgtgcag aatgccgggc ctccggagga ccttcgggcg cccgccccgc ccctgagccc
     7681 gcccctgagc ccgcccccgg acccacccct tcccagcctc tgagcccaga aagcgaagga
     7741 gcaaagctgc tattggccgc tgccccaaag gcctacccgc ttccattgct cagcggtgct
     7801 gtccatctgc acgagactag tgagacgtgc tacttccatt tgtcacgtcc tgcacgacgc
     7861 gagctgcggg gcggggggga acttcctgac taggggagga gtagaaggtg gcgcgaaggg
     7921 gccaccaaag aacggagccg gttggcgcct accggtggat gtggaatgtg tgcgaggcca
     7981 gaggccactt gtgtagcgcc aagtgcccag cggggctgct aaagcgcatg ctccagactg
     8041 ccttgggaaa agcgcctccc ctacccggta gatctcgatc ctctacgccg gacgcatcgt
     8101 ggccggagta ctgcggttta tctcgatggc tacgagggca gacagtaagt ggatttacca
     8161 taatccctta attgtacgca ccgctaaaac gcgttcagcg cgatcacggc agcagacagg
     8221 taaaaatggc aacaaaccac ccgaaaaact gccgcaatcg cgcctgataa attttaaccg
     8281 tatgaatacc tatgcaacca gagggtacag gccacattac ccccacttaa tccactgaag
     8341 ctgccatttt tcatggtttc accatcccag cgaagggcca tccagcgtgc gttcctgtat
     8401 ttccggacta taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     8461 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     8521 c
//
