LOCUS       Exported                9151 bp ds-DNA     circular SYN 04-OCT-2024
DEFINITION  7_1.
ACCESSION   7_1
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9151)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Mar 28, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     source          1..9151
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      1..589
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(747..1607)
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.5"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(1608..1712)
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             1755..1847
                     /label=tnpA (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=96.8"
                     /note="/match_length=6.5"
                     /note="/other=CDS"
     polyA_signal    complement(1868..1916)
                     /label=poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     CDS             complement(1980..2772)
                     /label=NeoR/KanR (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.7"
                     /note="/match_length=99.7"
                     /note="/other=CDS"
     CDS             complement(2823..3755)
                     /label=hRluc
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=99.7"
                     /note="/other=CDS"
     promoter        complement(3806..4163)
                     /label=SV40 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    4228..4279
                     /label=bom (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=36.9"
                     /note="/other=misc_feature"
     rep_origin      complement(4280..4312)
                     /direction=LEFT
                     /label=M13 ori (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.0"
                     /note="/match_length=7.3"
                     /note="/other=rep_origin"
     CDS             4317..4385
                     /label=large T antigen (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.1"
                     /note="/match_length=3.2"
                     /note="/other=CDS"
     polyA_signal    4408..4529
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     terminator      complement(4551..4570)
                     /label=T7 terminator (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=41.7"
                     /note="/other=terminator"
     CDS             complement(4578..4627)
                     /label=SET1 (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=39.7"
                     /note="/other=CDS"
     misc_feature    complement(4634..4657)
                     /label=MCS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=42.1"
                     /note="/other=misc_feature"
     CDS             complement(6498..8150)
                     /label=luciferase
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(8197..8696)
                     /label=PGK promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    complement(8753..9036)
                     /label=cer region
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
ORIGIN
        1 ttgaaatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccggttg
      721 ggcgtcgctt ggtcggtcat ttcgaattac caatgcttaa tcagtgaggc acctatctca
      781 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
      841 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
      901 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
      961 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     1021 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     1081 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     1141 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat agttgtcaga
     1201 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     1261 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     1321 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     1381 ccacatagca gaactttgaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     1441 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     1501 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     1561 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     1621 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     1681 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     1741 gtctaagaaa ccattacgta gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat
     1801 gtcagctact gggctatctg gacaagggaa aacgcaagcg caaagaggat ccttatcgct
     1861 atcgattcac acaaaaaacc aacacacaga tgtaatgaaa ataaagatat tttattgcgg
     1921 ccatcgtgat ggcaggttgg gcgtcgcttg gtcggtcatt tcgaacccca gagtcccgct
     1981 cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata
     2041 ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aatatcacgg
     2101 gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca gtcgatgaat
     2161 ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc atgggtcacg
     2221 acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg
     2281 agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc catccgagta
     2341 cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc
     2401 gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg agcaaggtga
     2461 gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct tcccgcttca
     2521 gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca cgatagccgc
     2581 gctgcctcgt cctgcagttc attcagggca ccggacaggt cggtcttgac aaaaagaacc
     2641 gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat tgtctgttgt
     2701 gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc gtgcaatcca
     2761 tcttgttcaa tccctccgga gcctccgcca cctgatccgc cacctccgct cccaccaccg
     2821 gtctgctcgt tcttcagcac gcgctccacg aagctcttga tgtacttacc catttcatct
     2881 ggagcgtcct cctggctgaa gtggaggccc ttcaccttca cgaactcggt gttagggaac
     2941 ttcttagctc cctcgacaat agcgttggaa aagaacccag ggtcggactc gatgaacatc
     3001 ttaggcagat cgtcgctggc ccgaaggtag gcgttgtagt tgcggacaat ctggacgacg
     3061 tcgggcttgc ctcccttaac gagagggatc tcgcgaggcc aggagagggt aggccgtcta
     3121 acctcgccct tctccttgaa tggctccagg taggcagcga actcctcagg ctccagtttc
     3181 cgcatgatct tgcttgggag catggtctcg acgaagaagt tattctcaag caccattttc
     3241 tcgccctctt cgctcttgat cagggcgata tcctcctcga tgtcaggcca ctcgtcccag
     3301 gactcgatca cgtccacgac actctcagca tggacgatgg ccttgatctt gtcttggtgc
     3361 tcgtaggagt agtgaaaggc cagacaagcc ccccagtcgt ggcccacaaa gatgattttc
     3421 tttggaaggt tcagcagctc gaaccaagcg gtgaggtact tgtagtgatc caggaggcga
     3481 tatgagccat tcccgctctt gccggactta cccattccga tcagatcagg gatgatgcat
     3541 ctagccacgg gctcgatgtg aggcacgacg tgcctccaca ggtagctgga ggcagcgtta
     3601 ccatgcagaa aaatcacggc gttctcggcg tgcttctcgg aatcatagta gttgatgaag
     3661 gagtccagca cgttcatttg cttgcagcga gcccaccact gaggcccagt gatcatgcgt
     3721 ttgcgttgct cggggtcgta caccttggaa gccatggtgg ctccagcctt tggttcgaga
     3781 ctgttgtgtc agaagaatca agctttttgc aaaagcctag gcctccaaaa aagcctcctc
     3841 actacttctg gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa
     3901 ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg
     3961 gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc
     4021 ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt
     4081 gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc
     4141 acagctggtt ctttccgcct cagaaggtac ctaaccaagt tcctctttca gaggttattt
     4201 caggccatgg tgctgcgcag atccgcgtat gcggtgtgaa ataccgcaca gatgcgtaag
     4261 gagaaaatac cgcatcagga aattgtaagc gttaatattt tgttaaaatt cgattttacc
     4321 acatttgtag aggttttact tgcttaaaaa aacctcccac atctccccct gaacctgaaa
     4381 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     4441 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     4501 ggtttgtcca aactcatcaa tgtatcttat catgtctgct cgaagcggcc gccccaaggg
     4561 gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt cgggctttgt
     4621 tagcagccgg atcagcttgc atgcctgcag gtcgactatt tttttctttt aaaatcacct
     4681 ttaatgctgt attctcccac aaataattta aagtgttgct cctatcttac ttatataaaa
     4741 caatcatata ctaatttgta gggtttttaa aaattctaaa catctacaat tttatgaaac
     4801 atactctcta tcccagtcct tagacaactt cttttgaggt attctataat gtgaaaatct
     4861 taagaaaaca ttaatcacaa acaaagccaa attataatca tgaatatgca tatgaacaaa
     4921 ggaaaaaaat ttctataatt tttgtaaccc cagttacaaa agtattatag accacaaata
     4981 aacttgtgga gattattcgg tattttgtct accccttaac actttccaca cagtgcctaa
     5041 tacatagtgt gtactgaaat atttgttaaa cacagtgaat tagggcaaac aatctggcac
     5101 aactttttgt attcaaatgc tatggagttt tcagaaaaca aaatctactt attttgtgat
     5161 ttgaagtgga aaaaaaacaa agcaacaaat gcagagctaa gtaaccatga ggttgatacc
     5221 atcactacaa cagtagttaa gatgtactgg ctctccactg taagtcattg aaagggcaaa
     5281 aattacgtag gtttgttaac attaacacat gctttattca agagataata ttcaaagagt
     5341 taaatcctaa gagttatcca ccctacagta aaaaggggaa gtgggtacca cttatgacat
     5401 gtacataaat tccactttta tatttctgaa taaagctgca attgcttctt tgatagagcc
     5461 atttcttaaa acttttgcta ataaggctat gtgaactgtg ttcagaaact ttgacaacat
     5521 gcacactcac tcctctcaaa gtcagtaccc aggattttca ctccaggctg agtacctatt
     5581 aagtaactag gacttcagac tgcatgttac tatatgaatt caatttgact cacctccagt
     5641 atgtttatct accacaacta ttgtttaaaa taatcagatg aatgtttatc ataactttat
     5701 aactccccca aattatactt cagtatttaa catggtagtt tcaaaaaata aatattcaag
     5761 gccccagttt taaattttcc tccaatgtaa tccacaaagt tgataataca tgttaggagc
     5821 actactctaa aataaatgta gctttctttt aaaattactt gttgcaatag agatcacatt
     5881 ctattccaaa ttctgataag gaatctgttt ttttggtaat acaacaaaaa tttgggatga
     5941 gtcagtcctt ggaaaatctt aatagtagtc actgaacgaa gtcaatcatt ccgagagcca
     6001 caaacactca aaaattactg ctagcattga caagttcttg tgttttttga tggttattgc
     6061 tttagttggc aatatctgaa tacaagacaa aagaacatct cccattaagc cttatgttaa
     6121 atattacaaa acttatacag aagatagact catagaggct ataaagctga tgtcttaagt
     6181 aactcaccca tgaaatgggt gcaggttttt taaaggctat ccaataatct ttttactcta
     6241 aaaataaatg cccctacttt tcagtgttcc cattcttcaa caggagactc gagcagctta
     6301 acagttttgc tataaaaaca aaccattctt cttaacaaac caagaatata aaaggaaacc
     6361 caataaagga cgtacttttc acagttaaca ataaataaaa gtgcaataag tgttcaagta
     6421 gggcatgtag tataaaaact acacatgagt atttagcata ggactcgagg ctagcgagct
     6481 cgtttaaaca actagaatta cacggcgatc ttgccgccct tcttggcctt aatgagaatc
     6541 tcgcggatct tgcgggcgtc caacttgccg gtcagtcctt taggcacctc gtccacgaac
     6601 acaacaccac cgcgcagctt cttggcggtt gtaacctggc tggccacata gtccacgatc
     6661 tccttctcgg tcatggtttt accgtgttcc agcacgacga ctgcggcggg cagctcgccg
     6721 gcatcgtcgt cgggcaggcc ggcgaccccg gcgtcgaaga tgttggggtg ttgcagcagg
     6781 atgctctcca gttcggctgg ggctacctgg tagcccttgt atttgatcag gctcttcagc
     6841 cggtccacga tgaagaagtg ctcgtcctcg tcccagtagg cgatgtcgcc gctgtgcagc
     6901 cagccgtcct tgtcgatgag agcgtttgta gcctcggggt tgttaacgta gccgctcatg
     6961 atcatggggc cacggacgca cagctcgccg cgctggttca cacccagtgt cttaccggtg
     7021 tccaagtcca ccaccttagc ctcgaagaag ggcaccacct tgcctactgc gccaggcttg
     7081 tcgtcccctt cgggggtgat cagaatggcg ctggttgttt ctgtcaggcc gtagccctgg
     7141 cggatgcctg gtaggtggaa gcgtttggcc acggcctcac ctacctcctt gctgagcggc
     7201 gccccgccgc tggcgatctc gtgcaagttg cttaggtcgt acttgtcgat gagagtgctc
     7261 ttagcgaaga agctaaatag tgtgggcacc agcagggcag attgaatctt atagtcttgc
     7321 aagctgcgca agaatagctc ctcctcgaag cggtacatga gcacgacccg aaagccgcag
     7381 atcaagtagc ccagcgtggt gaacatgccg aagccgtggt gaaatggcac cacgctgagg
     7441 atagcggtgt cggggatgat ctggttgccg aagatggggt cgcgggcatg actgaatcgg
     7501 acacaagcgg tgcggtgcgg tagggctacg cccttgggca atccggtact gccactactg
     7561 ttcatgatca gggcgatggt tttgtcccgg tcgaagctct cgggcacgaa gtcgtactcg
     7621 ttgaagccgg gtggcaaatg ggaagtcacg aaggtgtaca tgctttggaa gccctggtag
     7681 tcggtcttgc tatccatgat gatgatcttt tgtatgatcg gtagcttctt ttgcacgttg
     7741 aggatctttt gcagcccttt cttgctcacg aatacgacgg tgggctggct gatgcccatg
     7801 ctgttcagca gctcgcgctc gttgtagatg tcgttagctg gggccacagc cacaccgatg
     7861 aacagggcac ccaacacggg catgaagaac tgcaagctat tctcgctgca caccacgatc
     7921 cgatggtttg tattcagccc atagcgcttc atagcttctg ccagccgaac gctcatctcg
     7981 aagtactcgg cgtaggtaat gtccacctcg atatgtgcgt cggtaaaggc gatggtgccg
     8041 ggcaccaggg cgtagcgctt catggctttg tgcagctgct cgccggcggt cccgtcttcg
     8101 agtgggtaga atggcgctgg gcccttctta atgtttttgg catcttccat ggtggcttta
     8161 ccaacagtac cggattgcca agcttgggct gcaggtcgaa aggcccggag atgaggaaga
     8221 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     8281 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     8341 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     8401 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     8461 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     8521 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     8581 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     8641 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     8701 gatctcgatc ctctacgccg gacgcatcgt ggccggagta ctgcggttta tctcgatggc
     8761 tacgagggca gacagtaagt ggatttacca taatccctta attgtacgca ccgctaaaac
     8821 gcgttcagcg cgatcacggc agcagacagg taaaaatggc aacaaaccac ccgaaaaact
     8881 gccgcaatcg cgcctgataa attttaaccg tatgaatacc tatgcaacca gagggtacag
     8941 gccacattac ccccacttaa tccactgaag ctgccatttt tcatggtttc accatcccag
     9001 cgaagggcca tccagcgtgc gttcctgtat ttccggacta taaaaggatc taggtgaaga
     9061 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     9121 cagaccccgt agaaaagatc aaaggatctt c
//
