LOCUS       Exported                7829 bp ds-DNA     circular SYN 04-OCT-2024
DEFINITION  T19.
ACCESSION   T19
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Mar 28, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     source          1..7829
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      1..589
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(747..1607)
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.5"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(1608..1712)
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             1755..1847
                     /label=tnpA (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=96.8"
                     /note="/match_length=6.5"
                     /note="/other=CDS"
     polyA_signal    complement(1868..1916)
                     /label=poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     CDS             complement(1980..2772)
                     /label=NeoR/KanR (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.7"
                     /note="/match_length=99.7"
                     /note="/other=CDS"
     CDS             complement(2823..3755)
                     /label=hRluc
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=99.7"
                     /note="/other=CDS"
     promoter        complement(3806..4163)
                     /label=SV40 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    4228..4279
                     /label=bom (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=36.9"
                     /note="/other=misc_feature"
     rep_origin      complement(4280..4312)
                     /direction=LEFT
                     /label=M13 ori (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.0"
                     /note="/match_length=7.3"
                     /note="/other=rep_origin"
     CDS             4317..4385
                     /label=large T antigen (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.1"
                     /note="/match_length=3.2"
                     /note="/other=CDS"
     polyA_signal    4408..4529
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     terminator      complement(4551..4570)
                     /label=T7 terminator (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=41.7"
                     /note="/other=terminator"
     CDS             complement(4578..4627)
                     /label=SET1 (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=39.7"
                     /note="/other=CDS"
     misc_feature    complement(4634..4657)
                     /label=MCS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=42.1"
                     /note="/other=misc_feature"
     CDS             complement(5176..6828)
                     /label=luciferase
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(6875..7374)
                     /label=PGK promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    complement(7431..7714)
                     /label=cer region
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
ORIGIN
        1 ttgaaatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccggttg
      721 ggcgtcgctt ggtcggtcat ttcgaattac caatgcttaa tcagtgaggc acctatctca
      781 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
      841 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
      901 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
      961 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     1021 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     1081 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     1141 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat agttgtcaga
     1201 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     1261 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     1321 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     1381 ccacatagca gaactttgaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     1441 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     1501 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     1561 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     1621 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     1681 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     1741 gtctaagaaa ccattacgta gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat
     1801 gtcagctact gggctatctg gacaagggaa aacgcaagcg caaagaggat ccttatcgct
     1861 atcgattcac acaaaaaacc aacacacaga tgtaatgaaa ataaagatat tttattgcgg
     1921 ccatcgtgat ggcaggttgg gcgtcgcttg gtcggtcatt tcgaacccca gagtcccgct
     1981 cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata
     2041 ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aatatcacgg
     2101 gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca gtcgatgaat
     2161 ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc atgggtcacg
     2221 acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg
     2281 agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc catccgagta
     2341 cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc
     2401 gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg agcaaggtga
     2461 gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct tcccgcttca
     2521 gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca cgatagccgc
     2581 gctgcctcgt cctgcagttc attcagggca ccggacaggt cggtcttgac aaaaagaacc
     2641 gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat tgtctgttgt
     2701 gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc gtgcaatcca
     2761 tcttgttcaa tccctccgga gcctccgcca cctgatccgc cacctccgct cccaccaccg
     2821 gtctgctcgt tcttcagcac gcgctccacg aagctcttga tgtacttacc catttcatct
     2881 ggagcgtcct cctggctgaa gtggaggccc ttcaccttca cgaactcggt gttagggaac
     2941 ttcttagctc cctcgacaat agcgttggaa aagaacccag ggtcggactc gatgaacatc
     3001 ttaggcagat cgtcgctggc ccgaaggtag gcgttgtagt tgcggacaat ctggacgacg
     3061 tcgggcttgc ctcccttaac gagagggatc tcgcgaggcc aggagagggt aggccgtcta
     3121 acctcgccct tctccttgaa tggctccagg taggcagcga actcctcagg ctccagtttc
     3181 cgcatgatct tgcttgggag catggtctcg acgaagaagt tattctcaag caccattttc
     3241 tcgccctctt cgctcttgat cagggcgata tcctcctcga tgtcaggcca ctcgtcccag
     3301 gactcgatca cgtccacgac actctcagca tggacgatgg ccttgatctt gtcttggtgc
     3361 tcgtaggagt agtgaaaggc cagacaagcc ccccagtcgt ggcccacaaa gatgattttc
     3421 tttggaaggt tcagcagctc gaaccaagcg gtgaggtact tgtagtgatc caggaggcga
     3481 tatgagccat tcccgctctt gccggactta cccattccga tcagatcagg gatgatgcat
     3541 ctagccacgg gctcgatgtg aggcacgacg tgcctccaca ggtagctgga ggcagcgtta
     3601 ccatgcagaa aaatcacggc gttctcggcg tgcttctcgg aatcatagta gttgatgaag
     3661 gagtccagca cgttcatttg cttgcagcga gcccaccact gaggcccagt gatcatgcgt
     3721 ttgcgttgct cggggtcgta caccttggaa gccatggtgg ctccagcctt tggttcgaga
     3781 ctgttgtgtc agaagaatca agctttttgc aaaagcctag gcctccaaaa aagcctcctc
     3841 actacttctg gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa
     3901 ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg
     3961 gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc
     4021 ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt
     4081 gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc
     4141 acagctggtt ctttccgcct cagaaggtac ctaaccaagt tcctctttca gaggttattt
     4201 caggccatgg tgctgcgcag atccgcgtat gcggtgtgaa ataccgcaca gatgcgtaag
     4261 gagaaaatac cgcatcagga aattgtaagc gttaatattt tgttaaaatt cgattttacc
     4321 acatttgtag aggttttact tgcttaaaaa aacctcccac atctccccct gaacctgaaa
     4381 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     4441 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     4501 ggtttgtcca aactcatcaa tgtatcttat catgtctgct cgaagcggcc gccccaaggg
     4561 gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt cgggctttgt
     4621 tagcagccgg atcagcttgc atgcctgcag gtcgactgga gctgggacat gtgacaggct
     4681 gtggtggcac cactgagcct agaccatgga gccttggcat gcccagggca agtggggagg
     4741 ttctctgctc cccaggcctg ctcggtgact gaccccatca tacccaaagt gcaatctcac
     4801 ggccaggttc tatgccctgt ccaagcgtaa accctcttgg aaacttcttt tggggcaatt
     4861 ttcctgtggc cttcctggcc tctacttcca tgtgcgcagc cccacagacg ttgctgagca
     4921 actcgccagc ctcctgagct ccatgcccat caggactcta gcctctgacc ttgctgttga
     4981 ctctgaaatc aggatttgga agttttcgaa ttaggagtag agagatctga cctcttgcca
     5041 ggaatgccca tggatcatgt gattggcttt tctacccata gagggccttg cagcctgata
     5101 ccacctggga gtgagggtca caaaggagac cttggctccc actcgaggct agcgagctcg
     5161 tttaaacaac tagaattaca cggcgatctt gccgcccttc ttggccttaa tgagaatctc
     5221 gcggatcttg cgggcgtcca acttgccggt cagtccttta ggcacctcgt ccacgaacac
     5281 aacaccaccg cgcagcttct tggcggttgt aacctggctg gccacatagt ccacgatctc
     5341 cttctcggtc atggttttac cgtgttccag cacgacgact gcggcgggca gctcgccggc
     5401 atcgtcgtcg ggcaggccgg cgaccccggc gtcgaagatg ttggggtgtt gcagcaggat
     5461 gctctccagt tcggctgggg ctacctggta gcccttgtat ttgatcaggc tcttcagccg
     5521 gtccacgatg aagaagtgct cgtcctcgtc ccagtaggcg atgtcgccgc tgtgcagcca
     5581 gccgtccttg tcgatgagag cgtttgtagc ctcggggttg ttaacgtagc cgctcatgat
     5641 catggggcca cggacgcaca gctcgccgcg ctggttcaca cccagtgtct taccggtgtc
     5701 caagtccacc accttagcct cgaagaaggg caccaccttg cctactgcgc caggcttgtc
     5761 gtccccttcg ggggtgatca gaatggcgct ggttgtttct gtcaggccgt agccctggcg
     5821 gatgcctggt aggtggaagc gtttggccac ggcctcacct acctccttgc tgagcggcgc
     5881 cccgccgctg gcgatctcgt gcaagttgct taggtcgtac ttgtcgatga gagtgctctt
     5941 agcgaagaag ctaaatagtg tgggcaccag cagggcagat tgaatcttat agtcttgcaa
     6001 gctgcgcaag aatagctcct cctcgaagcg gtacatgagc acgacccgaa agccgcagat
     6061 caagtagccc agcgtggtga acatgccgaa gccgtggtga aatggcacca cgctgaggat
     6121 agcggtgtcg gggatgatct ggttgccgaa gatggggtcg cgggcatgac tgaatcggac
     6181 acaagcggtg cggtgcggta gggctacgcc cttgggcaat ccggtactgc cactactgtt
     6241 catgatcagg gcgatggttt tgtcccggtc gaagctctcg ggcacgaagt cgtactcgtt
     6301 gaagccgggt ggcaaatggg aagtcacgaa ggtgtacatg ctttggaagc cctggtagtc
     6361 ggtcttgcta tccatgatga tgatcttttg tatgatcggt agcttctttt gcacgttgag
     6421 gatcttttgc agccctttct tgctcacgaa tacgacggtg ggctggctga tgcccatgct
     6481 gttcagcagc tcgcgctcgt tgtagatgtc gttagctggg gccacagcca caccgatgaa
     6541 cagggcaccc aacacgggca tgaagaactg caagctattc tcgctgcaca ccacgatccg
     6601 atggtttgta ttcagcccat agcgcttcat agcttctgcc agccgaacgc tcatctcgaa
     6661 gtactcggcg taggtaatgt ccacctcgat atgtgcgtcg gtaaaggcga tggtgccggg
     6721 caccagggcg tagcgcttca tggctttgtg cagctgctcg ccggcggtcc cgtcttcgag
     6781 tgggtagaat ggcgctgggc ccttcttaat gtttttggca tcttccatgg tggctttacc
     6841 aacagtaccg gattgccaag cttgggctgc aggtcgaaag gcccggagat gaggaagagg
     6901 agaacagcgc ggcagacgtg cgcttttgaa gcgtgcagaa tgccgggcct ccggaggacc
     6961 ttcgggcgcc cgccccgccc ctgagcccgc ccctgagccc gcccccggac ccaccccttc
     7021 ccagcctctg agcccagaaa gcgaaggagc aaagctgcta ttggccgctg ccccaaaggc
     7081 ctacccgctt ccattgctca gcggtgctgt ccatctgcac gagactagtg agacgtgcta
     7141 cttccatttg tcacgtcctg cacgacgcga gctgcggggc gggggggaac ttcctgacta
     7201 ggggaggagt agaaggtggc gcgaaggggc caccaaagaa cggagccggt tggcgcctac
     7261 cggtggatgt ggaatgtgtg cgaggccaga ggccacttgt gtagcgccaa gtgcccagcg
     7321 gggctgctaa agcgcatgct ccagactgcc ttgggaaaag cgcctcccct acccggtaga
     7381 tctcgatcct ctacgccgga cgcatcgtgg ccggagtact gcggtttatc tcgatggcta
     7441 cgagggcaga cagtaagtgg atttaccata atcccttaat tgtacgcacc gctaaaacgc
     7501 gttcagcgcg atcacggcag cagacaggta aaaatggcaa caaaccaccc gaaaaactgc
     7561 cgcaatcgcg cctgataaat tttaaccgta tgaataccta tgcaaccaga gggtacaggc
     7621 cacattaccc ccacttaatc cactgaagct gccatttttc atggtttcac catcccagcg
     7681 aagggccatc cagcgtgcgt tcctgtattt ccggactata aaaggatcta ggtgaagatc
     7741 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     7801 gaccccgtag aaaagatcaa aggatcttc
//
