LOCUS       Exported                9291 bp ds-DNA     circular SYN 04-OCT-2024
DEFINITION  T20.
ACCESSION   T20
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9291)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Mar 28, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     source          1..9291
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      1..589
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(747..1607)
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.5"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(1608..1712)
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             1755..1847
                     /label=tnpA (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=96.8"
                     /note="/match_length=6.5"
                     /note="/other=CDS"
     polyA_signal    complement(1868..1916)
                     /label=poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     CDS             complement(1980..2772)
                     /label=NeoR/KanR (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.7"
                     /note="/match_length=99.7"
                     /note="/other=CDS"
     CDS             complement(2823..3755)
                     /label=hRluc
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=99.7"
                     /note="/other=CDS"
     promoter        complement(3806..4163)
                     /label=SV40 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    4228..4279
                     /label=bom (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=36.9"
                     /note="/other=misc_feature"
     rep_origin      complement(4280..4312)
                     /direction=LEFT
                     /label=M13 ori (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.0"
                     /note="/match_length=7.3"
                     /note="/other=rep_origin"
     CDS             4317..4385
                     /label=large T antigen (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.1"
                     /note="/match_length=3.2"
                     /note="/other=CDS"
     polyA_signal    4408..4529
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     terminator      complement(4551..4570)
                     /label=T7 terminator (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=41.7"
                     /note="/other=terminator"
     CDS             complement(4578..4627)
                     /label=SET1 (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=39.7"
                     /note="/other=CDS"
     misc_feature    complement(4634..4657)
                     /label=MCS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=42.1"
                     /note="/other=misc_feature"
     CDS             complement(6638..8290)
                     /label=luciferase
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(8337..8836)
                     /label=PGK promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    complement(8893..9176)
                     /label=cer region
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
ORIGIN
        1 ttgaaatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccggttg
      721 ggcgtcgctt ggtcggtcat ttcgaattac caatgcttaa tcagtgaggc acctatctca
      781 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
      841 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
      901 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
      961 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     1021 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     1081 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     1141 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat agttgtcaga
     1201 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     1261 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     1321 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     1381 ccacatagca gaactttgaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     1441 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     1501 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     1561 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     1621 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     1681 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     1741 gtctaagaaa ccattacgta gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat
     1801 gtcagctact gggctatctg gacaagggaa aacgcaagcg caaagaggat ccttatcgct
     1861 atcgattcac acaaaaaacc aacacacaga tgtaatgaaa ataaagatat tttattgcgg
     1921 ccatcgtgat ggcaggttgg gcgtcgcttg gtcggtcatt tcgaacccca gagtcccgct
     1981 cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata
     2041 ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aatatcacgg
     2101 gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca gtcgatgaat
     2161 ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc atgggtcacg
     2221 acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg
     2281 agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc catccgagta
     2341 cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc
     2401 gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg agcaaggtga
     2461 gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct tcccgcttca
     2521 gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca cgatagccgc
     2581 gctgcctcgt cctgcagttc attcagggca ccggacaggt cggtcttgac aaaaagaacc
     2641 gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat tgtctgttgt
     2701 gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc gtgcaatcca
     2761 tcttgttcaa tccctccgga gcctccgcca cctgatccgc cacctccgct cccaccaccg
     2821 gtctgctcgt tcttcagcac gcgctccacg aagctcttga tgtacttacc catttcatct
     2881 ggagcgtcct cctggctgaa gtggaggccc ttcaccttca cgaactcggt gttagggaac
     2941 ttcttagctc cctcgacaat agcgttggaa aagaacccag ggtcggactc gatgaacatc
     3001 ttaggcagat cgtcgctggc ccgaaggtag gcgttgtagt tgcggacaat ctggacgacg
     3061 tcgggcttgc ctcccttaac gagagggatc tcgcgaggcc aggagagggt aggccgtcta
     3121 acctcgccct tctccttgaa tggctccagg taggcagcga actcctcagg ctccagtttc
     3181 cgcatgatct tgcttgggag catggtctcg acgaagaagt tattctcaag caccattttc
     3241 tcgccctctt cgctcttgat cagggcgata tcctcctcga tgtcaggcca ctcgtcccag
     3301 gactcgatca cgtccacgac actctcagca tggacgatgg ccttgatctt gtcttggtgc
     3361 tcgtaggagt agtgaaaggc cagacaagcc ccccagtcgt ggcccacaaa gatgattttc
     3421 tttggaaggt tcagcagctc gaaccaagcg gtgaggtact tgtagtgatc caggaggcga
     3481 tatgagccat tcccgctctt gccggactta cccattccga tcagatcagg gatgatgcat
     3541 ctagccacgg gctcgatgtg aggcacgacg tgcctccaca ggtagctgga ggcagcgtta
     3601 ccatgcagaa aaatcacggc gttctcggcg tgcttctcgg aatcatagta gttgatgaag
     3661 gagtccagca cgttcatttg cttgcagcga gcccaccact gaggcccagt gatcatgcgt
     3721 ttgcgttgct cggggtcgta caccttggaa gccatggtgg ctccagcctt tggttcgaga
     3781 ctgttgtgtc agaagaatca agctttttgc aaaagcctag gcctccaaaa aagcctcctc
     3841 actacttctg gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa
     3901 ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg
     3961 gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc
     4021 ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt
     4081 gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc
     4141 acagctggtt ctttccgcct cagaaggtac ctaaccaagt tcctctttca gaggttattt
     4201 caggccatgg tgctgcgcag atccgcgtat gcggtgtgaa ataccgcaca gatgcgtaag
     4261 gagaaaatac cgcatcagga aattgtaagc gttaatattt tgttaaaatt cgattttacc
     4321 acatttgtag aggttttact tgcttaaaaa aacctcccac atctccccct gaacctgaaa
     4381 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     4441 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     4501 ggtttgtcca aactcatcaa tgtatcttat catgtctgct cgaagcggcc gccccaaggg
     4561 gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt cgggctttgt
     4621 tagcagccgg atcagcttgc atgcctgcag gtcgactgga gctgggacat gtgacaggct
     4681 gtggtggcac cactgagcct agaccatgga gccttggcat gcccagggca agtggggagg
     4741 ttctctgctc cccaggcctg ctcggtgact gaccccatca tacccaaagt gcaatctcac
     4801 ggccaggttc tatgccctgt ccaagcgtaa accctcttgg aaacttcttt tggggcaatt
     4861 ttcctgtggc cttcctggcc tctacttcca tgtgcgcagc cccacagacg ttgctgagca
     4921 actcgccagc ctcctgagct ccatgcccat caggactcta gcctctgacc ttgctgttga
     4981 ctctgaaatc aggatttgga agttttcgaa ttaggagtag agagatctga cctcttgcca
     5041 ggaatgccca tggatcatgt gattggcttt tctacccata gagggccttg cagcctgata
     5101 ccacctggga gtgagggtca caaaggagac cttggctccc tcaggtcacc aataaacctg
     5161 ttctttaatc aagtagttgt aatatggacc tggggtacct ttagttaggg actctggtgg
     5221 cactacacgg ggtgctggat tcaaatctca cctctatgga tgatgtctgg catcgtctgg
     5281 cccctccttt tccaaagtga ccagaagatt gtacttccta gagatgccag acttgcctca
     5341 cacccaggag ctgcccctgc tgtgcctggt atgctggcag tggtgatggt gacaacacaa
     5401 tgaggccaag tagcaatgct cccaggtcct gcagcttgtg ctctgcccag cagtgcccct
     5461 ggcccagcac ctcagcctgc tcttctctct ggtggctcca ccccaacccc tacttctttt
     5521 cctttatttc tctcccaacc tggctgcaaa ggaggttcat attccccaga tgtgtccaca
     5581 aacctgtcca ggagttgtgt cttaaaattg atttcctgtc cctcttccta aggacagggg
     5641 taacagtcaa agtgaaggaa tgatgggttc ttgcctgtct tgctttggtc cgtatttccc
     5701 aagccctcct cttttagcca aggctacagg gcacttgtct tccctggtgg ctctcccctt
     5761 tggagagagc tagtggcaga gcaacctagc taggtcctcc ttgggctttg aatgcaggag
     5821 cacccagtgc cccaggcctt gcactgtgct gcccaaggag tattttctgg gtggatctcc
     5881 catactcctc agggttctga ggtccttgta tttagggtat ggtgtaaaaa cttacatatg
     5941 cacattttgt tacatcattt aagatgattg aagctttact ggttaagtat agggtcacca
     6001 gagaaagttc ctttccccag ttgatgtctt gaggctgttg agaaacttag gacagaagcc
     6061 tacctcatcc caggcctgcc cctccgtgca tagctctgcc tttgggtctt gttcatagtg
     6121 ctgttcaggc aaaatggaat agtctcaggt gggaacacct tctcttgcta gctccaggaa
     6181 ggctttgtgg gaaatgagtt gagaccatgg acttgggagt tgggggccag agttgagcct
     6241 ggacttgacc aattaattcc cttacctttc agcccaagat agctacatgt ctttacttgc
     6301 tgtataagtt ttccttttgt cctgggggtg cctgattgat cctatctctt cacccttcat
     6361 tctttcaaca aacatgccat ctatctccca ggacatttat tcccttctat tcaaggccag
     6421 ttaggaatgc aattattttt ttttttcagt taaatacaga cttgtttgga cgcaaggtac
     6481 cctttctctt tttaaaaact ttttatcatg aaaactttca aatacaaaaa taaaatggta
     6541 gagtgaacct ccatgtaccc agcatccagc tccagtaacc atcaacgtgt accattattt
     6601 caactcgagg ctagcgagct cgtttaaaca actagaatta cacggcgatc ttgccgccct
     6661 tcttggcctt aatgagaatc tcgcggatct tgcgggcgtc caacttgccg gtcagtcctt
     6721 taggcacctc gtccacgaac acaacaccac cgcgcagctt cttggcggtt gtaacctggc
     6781 tggccacata gtccacgatc tccttctcgg tcatggtttt accgtgttcc agcacgacga
     6841 ctgcggcggg cagctcgccg gcatcgtcgt cgggcaggcc ggcgaccccg gcgtcgaaga
     6901 tgttggggtg ttgcagcagg atgctctcca gttcggctgg ggctacctgg tagcccttgt
     6961 atttgatcag gctcttcagc cggtccacga tgaagaagtg ctcgtcctcg tcccagtagg
     7021 cgatgtcgcc gctgtgcagc cagccgtcct tgtcgatgag agcgtttgta gcctcggggt
     7081 tgttaacgta gccgctcatg atcatggggc cacggacgca cagctcgccg cgctggttca
     7141 cacccagtgt cttaccggtg tccaagtcca ccaccttagc ctcgaagaag ggcaccacct
     7201 tgcctactgc gccaggcttg tcgtcccctt cgggggtgat cagaatggcg ctggttgttt
     7261 ctgtcaggcc gtagccctgg cggatgcctg gtaggtggaa gcgtttggcc acggcctcac
     7321 ctacctcctt gctgagcggc gccccgccgc tggcgatctc gtgcaagttg cttaggtcgt
     7381 acttgtcgat gagagtgctc ttagcgaaga agctaaatag tgtgggcacc agcagggcag
     7441 attgaatctt atagtcttgc aagctgcgca agaatagctc ctcctcgaag cggtacatga
     7501 gcacgacccg aaagccgcag atcaagtagc ccagcgtggt gaacatgccg aagccgtggt
     7561 gaaatggcac cacgctgagg atagcggtgt cggggatgat ctggttgccg aagatggggt
     7621 cgcgggcatg actgaatcgg acacaagcgg tgcggtgcgg tagggctacg cccttgggca
     7681 atccggtact gccactactg ttcatgatca gggcgatggt tttgtcccgg tcgaagctct
     7741 cgggcacgaa gtcgtactcg ttgaagccgg gtggcaaatg ggaagtcacg aaggtgtaca
     7801 tgctttggaa gccctggtag tcggtcttgc tatccatgat gatgatcttt tgtatgatcg
     7861 gtagcttctt ttgcacgttg aggatctttt gcagcccttt cttgctcacg aatacgacgg
     7921 tgggctggct gatgcccatg ctgttcagca gctcgcgctc gttgtagatg tcgttagctg
     7981 gggccacagc cacaccgatg aacagggcac ccaacacggg catgaagaac tgcaagctat
     8041 tctcgctgca caccacgatc cgatggtttg tattcagccc atagcgcttc atagcttctg
     8101 ccagccgaac gctcatctcg aagtactcgg cgtaggtaat gtccacctcg atatgtgcgt
     8161 cggtaaaggc gatggtgccg ggcaccaggg cgtagcgctt catggctttg tgcagctgct
     8221 cgccggcggt cccgtcttcg agtgggtaga atggcgctgg gcccttctta atgtttttgg
     8281 catcttccat ggtggcttta ccaacagtac cggattgcca agcttgggct gcaggtcgaa
     8341 aggcccggag atgaggaaga ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag
     8401 aatgccgggc ctccggagga ccttcgggcg cccgccccgc ccctgagccc gcccctgagc
     8461 ccgcccccgg acccacccct tcccagcctc tgagcccaga aagcgaagga gcaaagctgc
     8521 tattggccgc tgccccaaag gcctacccgc ttccattgct cagcggtgct gtccatctgc
     8581 acgagactag tgagacgtgc tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg
     8641 gcggggggga acttcctgac taggggagga gtagaaggtg gcgcgaaggg gccaccaaag
     8701 aacggagccg gttggcgcct accggtggat gtggaatgtg tgcgaggcca gaggccactt
     8761 gtgtagcgcc aagtgcccag cggggctgct aaagcgcatg ctccagactg ccttgggaaa
     8821 agcgcctccc ctacccggta gatctcgatc ctctacgccg gacgcatcgt ggccggagta
     8881 ctgcggttta tctcgatggc tacgagggca gacagtaagt ggatttacca taatccctta
     8941 attgtacgca ccgctaaaac gcgttcagcg cgatcacggc agcagacagg taaaaatggc
     9001 aacaaaccac ccgaaaaact gccgcaatcg cgcctgataa attttaaccg tatgaatacc
     9061 tatgcaacca gagggtacag gccacattac ccccacttaa tccactgaag ctgccatttt
     9121 tcatggtttc accatcccag cgaagggcca tccagcgtgc gttcctgtat ttccggacta
     9181 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     9241 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt c
//
