LOCUS       Exported                9687 bp ds-DNA     circular SYN 04-OCT-2024
DEFINITION  T22.
ACCESSION   T22
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Mar 28, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     source          1..9687
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      1..589
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(747..1607)
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.5"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(1608..1712)
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             1755..1847
                     /label=tnpA (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=96.8"
                     /note="/match_length=6.5"
                     /note="/other=CDS"
     polyA_signal    complement(1868..1916)
                     /label=poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     CDS             complement(1980..2772)
                     /label=NeoR/KanR (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.7"
                     /note="/match_length=99.7"
                     /note="/other=CDS"
     CDS             complement(2823..3755)
                     /label=hRluc
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=99.7"
                     /note="/other=CDS"
     promoter        complement(3806..4163)
                     /label=SV40 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    4228..4279
                     /label=bom (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=36.9"
                     /note="/other=misc_feature"
     rep_origin      complement(4280..4312)
                     /direction=LEFT
                     /label=M13 ori (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.0"
                     /note="/match_length=7.3"
                     /note="/other=rep_origin"
     CDS             4317..4385
                     /label=large T antigen (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.1"
                     /note="/match_length=3.2"
                     /note="/other=CDS"
     polyA_signal    4408..4529
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     terminator      complement(4551..4570)
                     /label=T7 terminator (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=41.7"
                     /note="/other=terminator"
     CDS             complement(4578..4627)
                     /label=SET1 (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=39.7"
                     /note="/other=CDS"
     misc_feature    complement(4634..4657)
                     /label=MCS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=42.1"
                     /note="/other=misc_feature"
     CDS             5410..5691
                     /label=GVQW1 (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=66.0"
                     /note="/match_length=48.2"
                     /note="/other=CDS"
     CDS             complement(7034..8686)
                     /label=luciferase
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(8733..9232)
                     /label=PGK promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    complement(9289..9572)
                     /label=cer region
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
ORIGIN
        1 ttgaaatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccggttg
      721 ggcgtcgctt ggtcggtcat ttcgaattac caatgcttaa tcagtgaggc acctatctca
      781 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
      841 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
      901 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
      961 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     1021 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     1081 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     1141 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat agttgtcaga
     1201 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     1261 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     1321 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     1381 ccacatagca gaactttgaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     1441 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     1501 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     1561 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     1621 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     1681 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     1741 gtctaagaaa ccattacgta gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat
     1801 gtcagctact gggctatctg gacaagggaa aacgcaagcg caaagaggat ccttatcgct
     1861 atcgattcac acaaaaaacc aacacacaga tgtaatgaaa ataaagatat tttattgcgg
     1921 ccatcgtgat ggcaggttgg gcgtcgcttg gtcggtcatt tcgaacccca gagtcccgct
     1981 cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata
     2041 ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aatatcacgg
     2101 gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca gtcgatgaat
     2161 ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc atgggtcacg
     2221 acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg
     2281 agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc catccgagta
     2341 cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc
     2401 gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg agcaaggtga
     2461 gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct tcccgcttca
     2521 gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca cgatagccgc
     2581 gctgcctcgt cctgcagttc attcagggca ccggacaggt cggtcttgac aaaaagaacc
     2641 gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat tgtctgttgt
     2701 gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc gtgcaatcca
     2761 tcttgttcaa tccctccgga gcctccgcca cctgatccgc cacctccgct cccaccaccg
     2821 gtctgctcgt tcttcagcac gcgctccacg aagctcttga tgtacttacc catttcatct
     2881 ggagcgtcct cctggctgaa gtggaggccc ttcaccttca cgaactcggt gttagggaac
     2941 ttcttagctc cctcgacaat agcgttggaa aagaacccag ggtcggactc gatgaacatc
     3001 ttaggcagat cgtcgctggc ccgaaggtag gcgttgtagt tgcggacaat ctggacgacg
     3061 tcgggcttgc ctcccttaac gagagggatc tcgcgaggcc aggagagggt aggccgtcta
     3121 acctcgccct tctccttgaa tggctccagg taggcagcga actcctcagg ctccagtttc
     3181 cgcatgatct tgcttgggag catggtctcg acgaagaagt tattctcaag caccattttc
     3241 tcgccctctt cgctcttgat cagggcgata tcctcctcga tgtcaggcca ctcgtcccag
     3301 gactcgatca cgtccacgac actctcagca tggacgatgg ccttgatctt gtcttggtgc
     3361 tcgtaggagt agtgaaaggc cagacaagcc ccccagtcgt ggcccacaaa gatgattttc
     3421 tttggaaggt tcagcagctc gaaccaagcg gtgaggtact tgtagtgatc caggaggcga
     3481 tatgagccat tcccgctctt gccggactta cccattccga tcagatcagg gatgatgcat
     3541 ctagccacgg gctcgatgtg aggcacgacg tgcctccaca ggtagctgga ggcagcgtta
     3601 ccatgcagaa aaatcacggc gttctcggcg tgcttctcgg aatcatagta gttgatgaag
     3661 gagtccagca cgttcatttg cttgcagcga gcccaccact gaggcccagt gatcatgcgt
     3721 ttgcgttgct cggggtcgta caccttggaa gccatggtgg ctccagcctt tggttcgaga
     3781 ctgttgtgtc agaagaatca agctttttgc aaaagcctag gcctccaaaa aagcctcctc
     3841 actacttctg gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa
     3901 ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg
     3961 gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc
     4021 ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt
     4081 gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc
     4141 acagctggtt ctttccgcct cagaaggtac ctaaccaagt tcctctttca gaggttattt
     4201 caggccatgg tgctgcgcag atccgcgtat gcggtgtgaa ataccgcaca gatgcgtaag
     4261 gagaaaatac cgcatcagga aattgtaagc gttaatattt tgttaaaatt cgattttacc
     4321 acatttgtag aggttttact tgcttaaaaa aacctcccac atctccccct gaacctgaaa
     4381 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     4441 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     4501 ggtttgtcca aactcatcaa tgtatcttat catgtctgct cgaagcggcc gccccaaggg
     4561 gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt cgggctttgt
     4621 tagcagccgg atcagcttgc atgcctgcag gtcgacttca ctgaatgcat aaagtccttt
     4681 attgaaaata ttgggatagc actgcattac atatagtcaa tatccataaa tgaagggtca
     4741 cacatttctg aatggacaat actgttttac atagagaaca cagcatctgg atatgctctc
     4801 acaattatag tatcatggac taaactaggt cagagtgaag tatatgcaaa atgaccattt
     4861 tgtttttttc cattttatta atagcatatg gttgcagatg gtgtaaatgg taaacgtgat
     4921 atcatgagac attcctgata tctcacacca acacattatt taacgagcag gttaaggtga
     4981 aactgccagt atgctgttag tcaagagtcc tcagtaggag aacttgagtg aaacgtacac
     5041 ccaggctaca gatttaaaat tttatagcat tcatttaaat ttgcatggaa ttaaggagta
     5101 taataattgt tgatctgaca ggaggccact ggacaatttg ttctctttca caaaatgtct
     5161 aaaataggag aataatgtaa aggaagtatt ctaagaattg cttttttaaa tgcagatatg
     5221 taaatagtac ttgttataca tttttccttt caaaaattac cggggaaata atttatgaca
     5281 gaccattttt cagctttggt tattatttga aattttttcc tactagcaag cagtctttgg
     5341 ctttcataaa agtctggtaa tcaataatat tggttaagta agtctttatt attattatta
     5401 ttgttattat ttttaagaca gagtctcgct gttacccagg atagagtgca gtggcacgat
     5461 cttggctcac tacaacctct gcttcccgga ttcaagcaat tctcctgcct cagcctcccg
     5521 agtaggtggg attacaggtg cccgccacat agtcggctaa tatttgtact tttagtagag
     5581 atggggtttc accatgttgg ccagactggt ctcaaacttg tgaccttgag tgatccacct
     5641 gcctcggcct cccaaagtgc tgggattaca ggcaagagcc actatgcctg gacctttatt
     5701 ttatgtaata gatgttttat gatgtgaaaa taggttgata tcttaaaaat aggttttcct
     5761 ttctattcca attttgatat gaatgcattt ctttgtattg tttttttctc aaataaatga
     5821 atcaagaaac caagacaatt ggtccaattt gacgtttttc atttgattta aaaaatgcag
     5881 aaattaatat actgagtccc ttttaagaca gaggcctgct cgttaatgta aaagcttgga
     5941 aaagttatac aaagaacact ccgaaaataa agtttttgtt attaacacaa atgattttgt
     6001 aagaagcaaa acattttttt tcaaaagcag gatttgttct aagtctaggt tcacaaaggt
     6061 attcatttct ataaatatca acttaagact gtgagaaatg atgtattctt tttcacacga
     6121 atatgttcaa ggtaagtaac taaaaatgta cgcttttatt gactaaatgg aaagctggcc
     6181 tataatagtc aaattgatga acattcataa tgagttttta gaaatttaga attatacact
     6241 catatatatg tatatgtaca tatacataca cacacacata tatctctata tacccacata
     6301 cagatatata gatatatgct gcttaagacc tatcacccta aacaatcagg gcaaaaaaaa
     6361 ttcaatataa aactatataa atcaaacaga tttgttccca aaacatagca ccaaaatgta
     6421 tacatttaat atttcaagat ttgacacatt aaaagaaaaa aaactgccga cacatttcag
     6481 acaatatact acaaataata aataaaagtt tttttttaaa aaaaaaaaaa cctcatatat
     6541 tccctttaag tgctgtgcac tttcgatttt tctaaatgag actaaccttt tccatggcaa
     6601 ataatagctg tacattttgg taatgaaaat tatgctgata aatacacaag gcatgtatat
     6661 atccacagta tcttattttt cacagttacc aaaaataaaa attgcattca actcatatat
     6721 ctataaacac tctgattcct cactgtatgg atttggagga agtgggagta caaagtcttt
     6781 tcaacaacct ggattttaag tttctttctt caatgtctgc cctctacatg gaccttccag
     6841 aatctttctg caatgtgatc atgctctgag atgatggcag aaaggaagca ttggggtggg
     6901 atgaggcagg ctgggctggg gcttcttttt ccagccagct ttaggagggc tgatcgaacc
     6961 cagtcttttt aatgttatat tttaaggcag tctgaattac tcgaggctag cgagctcgtt
     7021 taaacaacta gaattacacg gcgatcttgc cgcccttctt ggccttaatg agaatctcgc
     7081 ggatcttgcg ggcgtccaac ttgccggtca gtcctttagg cacctcgtcc acgaacacaa
     7141 caccaccgcg cagcttcttg gcggttgtaa cctggctggc cacatagtcc acgatctcct
     7201 tctcggtcat ggttttaccg tgttccagca cgacgactgc ggcgggcagc tcgccggcat
     7261 cgtcgtcggg caggccggcg accccggcgt cgaagatgtt ggggtgttgc agcaggatgc
     7321 tctccagttc ggctggggct acctggtagc ccttgtattt gatcaggctc ttcagccggt
     7381 ccacgatgaa gaagtgctcg tcctcgtccc agtaggcgat gtcgccgctg tgcagccagc
     7441 cgtccttgtc gatgagagcg tttgtagcct cggggttgtt aacgtagccg ctcatgatca
     7501 tggggccacg gacgcacagc tcgccgcgct ggttcacacc cagtgtctta ccggtgtcca
     7561 agtccaccac cttagcctcg aagaagggca ccaccttgcc tactgcgcca ggcttgtcgt
     7621 ccccttcggg ggtgatcaga atggcgctgg ttgtttctgt caggccgtag ccctggcgga
     7681 tgcctggtag gtggaagcgt ttggccacgg cctcacctac ctccttgctg agcggcgccc
     7741 cgccgctggc gatctcgtgc aagttgctta ggtcgtactt gtcgatgaga gtgctcttag
     7801 cgaagaagct aaatagtgtg ggcaccagca gggcagattg aatcttatag tcttgcaagc
     7861 tgcgcaagaa tagctcctcc tcgaagcggt acatgagcac gacccgaaag ccgcagatca
     7921 agtagcccag cgtggtgaac atgccgaagc cgtggtgaaa tggcaccacg ctgaggatag
     7981 cggtgtcggg gatgatctgg ttgccgaaga tggggtcgcg ggcatgactg aatcggacac
     8041 aagcggtgcg gtgcggtagg gctacgccct tgggcaatcc ggtactgcca ctactgttca
     8101 tgatcagggc gatggttttg tcccggtcga agctctcggg cacgaagtcg tactcgttga
     8161 agccgggtgg caaatgggaa gtcacgaagg tgtacatgct ttggaagccc tggtagtcgg
     8221 tcttgctatc catgatgatg atcttttgta tgatcggtag cttcttttgc acgttgagga
     8281 tcttttgcag ccctttcttg ctcacgaata cgacggtggg ctggctgatg cccatgctgt
     8341 tcagcagctc gcgctcgttg tagatgtcgt tagctggggc cacagccaca ccgatgaaca
     8401 gggcacccaa cacgggcatg aagaactgca agctattctc gctgcacacc acgatccgat
     8461 ggtttgtatt cagcccatag cgcttcatag cttctgccag ccgaacgctc atctcgaagt
     8521 actcggcgta ggtaatgtcc acctcgatat gtgcgtcggt aaaggcgatg gtgccgggca
     8581 ccagggcgta gcgcttcatg gctttgtgca gctgctcgcc ggcggtcccg tcttcgagtg
     8641 ggtagaatgg cgctgggccc ttcttaatgt ttttggcatc ttccatggtg gctttaccaa
     8701 cagtaccgga ttgccaagct tgggctgcag gtcgaaaggc ccggagatga ggaagaggag
     8761 aacagcgcgg cagacgtgcg cttttgaagc gtgcagaatg ccgggcctcc ggaggacctt
     8821 cgggcgcccg ccccgcccct gagcccgccc ctgagcccgc ccccggaccc accccttccc
     8881 agcctctgag cccagaaagc gaaggagcaa agctgctatt ggccgctgcc ccaaaggcct
     8941 acccgcttcc attgctcagc ggtgctgtcc atctgcacga gactagtgag acgtgctact
     9001 tccatttgtc acgtcctgca cgacgcgagc tgcggggcgg gggggaactt cctgactagg
     9061 ggaggagtag aaggtggcgc gaaggggcca ccaaagaacg gagccggttg gcgcctaccg
     9121 gtggatgtgg aatgtgtgcg aggccagagg ccacttgtgt agcgccaagt gcccagcggg
     9181 gctgctaaag cgcatgctcc agactgcctt gggaaaagcg cctcccctac ccggtagatc
     9241 tcgatcctct acgccggacg catcgtggcc ggagtactgc ggtttatctc gatggctacg
     9301 agggcagaca gtaagtggat ttaccataat cccttaattg tacgcaccgc taaaacgcgt
     9361 tcagcgcgat cacggcagca gacaggtaaa aatggcaaca aaccacccga aaaactgccg
     9421 caatcgcgcc tgataaattt taaccgtatg aatacctatg caaccagagg gtacaggcca
     9481 cattaccccc acttaatcca ctgaagctgc catttttcat ggtttcacca tcccagcgaa
     9541 gggccatcca gcgtgcgttc ctgtatttcc ggactataaa aggatctagg tgaagatcct
     9601 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     9661 ccccgtagaa aagatcaaag gatcttc
//
