LOCUS       Exported                8788 bp ds-DNA     circular SYN 04-OCT-2024
DEFINITION  T25.
ACCESSION   T25
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Mar 28, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     source          1..8788
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      1..589
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(747..1607)
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.5"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(1608..1712)
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             1755..1847
                     /label=tnpA (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=96.8"
                     /note="/match_length=6.5"
                     /note="/other=CDS"
     polyA_signal    complement(1868..1916)
                     /label=poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     CDS             complement(1980..2772)
                     /label=NeoR/KanR (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.7"
                     /note="/match_length=99.7"
                     /note="/other=CDS"
     CDS             complement(2823..3755)
                     /label=hRluc
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=99.7"
                     /note="/other=CDS"
     promoter        complement(3806..4163)
                     /label=SV40 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    4228..4279
                     /label=bom (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=36.9"
                     /note="/other=misc_feature"
     rep_origin      complement(4280..4312)
                     /direction=LEFT
                     /label=M13 ori (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.0"
                     /note="/match_length=7.3"
                     /note="/other=rep_origin"
     CDS             4317..4385
                     /label=large T antigen (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.1"
                     /note="/match_length=3.2"
                     /note="/other=CDS"
     polyA_signal    4408..4529
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     terminator      complement(4551..4570)
                     /label=T7 terminator (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=41.7"
                     /note="/other=terminator"
     CDS             complement(4578..4627)
                     /label=SET1 (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=39.7"
                     /note="/other=CDS"
     misc_feature    complement(4634..4657)
                     /label=MCS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=42.1"
                     /note="/other=misc_feature"
     CDS             5695..5889
                     /label=C9orf85 (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=69.2"
                     /note="/match_length=36.3"
                     /note="/other=CDS"
     CDS             5904..5969
                     /label=C14orf178 (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=81.8"
                     /note="/match_length=18.0"
                     /note="/other=CDS"
     CDS             complement(6135..7787)
                     /label=luciferase
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(7834..8333)
                     /label=PGK promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    complement(8390..8673)
                     /label=cer region
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
ORIGIN
        1 ttgaaatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccggttg
      721 ggcgtcgctt ggtcggtcat ttcgaattac caatgcttaa tcagtgaggc acctatctca
      781 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
      841 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
      901 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
      961 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     1021 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     1081 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     1141 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat agttgtcaga
     1201 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     1261 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     1321 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     1381 ccacatagca gaactttgaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     1441 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     1501 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     1561 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     1621 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     1681 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     1741 gtctaagaaa ccattacgta gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat
     1801 gtcagctact gggctatctg gacaagggaa aacgcaagcg caaagaggat ccttatcgct
     1861 atcgattcac acaaaaaacc aacacacaga tgtaatgaaa ataaagatat tttattgcgg
     1921 ccatcgtgat ggcaggttgg gcgtcgcttg gtcggtcatt tcgaacccca gagtcccgct
     1981 cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata
     2041 ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aatatcacgg
     2101 gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca gtcgatgaat
     2161 ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc atgggtcacg
     2221 acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg
     2281 agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc catccgagta
     2341 cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc
     2401 gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg agcaaggtga
     2461 gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct tcccgcttca
     2521 gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca cgatagccgc
     2581 gctgcctcgt cctgcagttc attcagggca ccggacaggt cggtcttgac aaaaagaacc
     2641 gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat tgtctgttgt
     2701 gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc gtgcaatcca
     2761 tcttgttcaa tccctccgga gcctccgcca cctgatccgc cacctccgct cccaccaccg
     2821 gtctgctcgt tcttcagcac gcgctccacg aagctcttga tgtacttacc catttcatct
     2881 ggagcgtcct cctggctgaa gtggaggccc ttcaccttca cgaactcggt gttagggaac
     2941 ttcttagctc cctcgacaat agcgttggaa aagaacccag ggtcggactc gatgaacatc
     3001 ttaggcagat cgtcgctggc ccgaaggtag gcgttgtagt tgcggacaat ctggacgacg
     3061 tcgggcttgc ctcccttaac gagagggatc tcgcgaggcc aggagagggt aggccgtcta
     3121 acctcgccct tctccttgaa tggctccagg taggcagcga actcctcagg ctccagtttc
     3181 cgcatgatct tgcttgggag catggtctcg acgaagaagt tattctcaag caccattttc
     3241 tcgccctctt cgctcttgat cagggcgata tcctcctcga tgtcaggcca ctcgtcccag
     3301 gactcgatca cgtccacgac actctcagca tggacgatgg ccttgatctt gtcttggtgc
     3361 tcgtaggagt agtgaaaggc cagacaagcc ccccagtcgt ggcccacaaa gatgattttc
     3421 tttggaaggt tcagcagctc gaaccaagcg gtgaggtact tgtagtgatc caggaggcga
     3481 tatgagccat tcccgctctt gccggactta cccattccga tcagatcagg gatgatgcat
     3541 ctagccacgg gctcgatgtg aggcacgacg tgcctccaca ggtagctgga ggcagcgtta
     3601 ccatgcagaa aaatcacggc gttctcggcg tgcttctcgg aatcatagta gttgatgaag
     3661 gagtccagca cgttcatttg cttgcagcga gcccaccact gaggcccagt gatcatgcgt
     3721 ttgcgttgct cggggtcgta caccttggaa gccatggtgg ctccagcctt tggttcgaga
     3781 ctgttgtgtc agaagaatca agctttttgc aaaagcctag gcctccaaaa aagcctcctc
     3841 actacttctg gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa
     3901 ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg
     3961 gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc
     4021 ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt
     4081 gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc
     4141 acagctggtt ctttccgcct cagaaggtac ctaaccaagt tcctctttca gaggttattt
     4201 caggccatgg tgctgcgcag atccgcgtat gcggtgtgaa ataccgcaca gatgcgtaag
     4261 gagaaaatac cgcatcagga aattgtaagc gttaatattt tgttaaaatt cgattttacc
     4321 acatttgtag aggttttact tgcttaaaaa aacctcccac atctccccct gaacctgaaa
     4381 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     4441 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     4501 ggtttgtcca aactcatcaa tgtatcttat catgtctgct cgaagcggcc gccccaaggg
     4561 gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt cgggctttgt
     4621 tagcagccgg atcagcttgc atgcctgcag gtcgactttt aaacaaaaga gatcttttaa
     4681 ttttttctta aatgctttag tatctcaatg cataaacaca ctgtttactt ttacagctgg
     4741 tccagtgtta tttactcttg gcaaattgta gacttacaaa acaaggcaaa tccctaccta
     4801 ttcaccttct cagcaagaat gacttctaac tcacacagca catactgggc tccagtaaag
     4861 ggatctgctc atatccatag aactaatgaa agatatatga ctaagtatac agcttaaatg
     4921 aagccaaatg agcttaatac agaaaatata tatgatgata ttaatcatat ataaacaatg
     4981 taaaagattc ataagcaaaa ttttattttg ccatcactaa ttcttgtgat tatgaagata
     5041 taacctaacc taggaagaaa ataggcagct gttcttttta gaaattaatt atctactgta
     5101 caagcccaca ggcctgcagg tacaatgaaa gcatgaaggt aacttcttta cactgtgaat
     5161 gggtaaagaa atccaatgag gccaatgtta tcggtttcat cgatgccaat attttttcac
     5221 ttgtcttgat attaagtaaa ggctaactgc ctatgtgaaa actttggtaa aattattgtg
     5281 gctgcaataa aactacaaca tacacacata acctccaaca gatccatttt aaatgaagaa
     5341 aaacaactac aattcgtttt taaaaatgct tcaaaaacag cacatactgt ggccacttca
     5401 ttgttcctgc atccctaaaa ggttcttaaa attaatcagt gaggtttctt ccatctaatt
     5461 acatgaactt tgtcacatga tgtcacatgc tagctaactg catgagcagt gcagctgctc
     5521 gcatcaacca aacataaaaa atattaaaat tattatcaga agagtagtag gtaaggattc
     5581 tactttgcga gttgatggaa ctccaccaaa ggtgttctac gtagaacagg taccttttct
     5641 tctccttcct tccttctcct tccttccttt ttttctttct ttctttcgag acagagtctc
     5701 cctctgtcac ccaggctgga gtgcagtggt gtcgtctcgg ctcactgcaa ctgccacttc
     5761 ctgggttcaa gcaattctcc tgcctcaacc tcgcgagtag ctgcgactac aggcatgcgc
     5821 caccatgctc ggctaatttt tgtaattttg gtagagacag ggttccgcca tgttggccag
     5881 gctggtctca aaccctgacc ccaagtgatc cacccacctc gacctcccaa agtgctggga
     5941 ttacaggcat gagccactgt gcccggcctg ttttctttct tttaaaacaa gaataatgag
     6001 gcaaaatatt tgagtgaaaa caaataacac aaaagcacaa ctcttggagg aaaaaatctg
     6061 acatttacca acagttttgg ggaggtgata catcaacaca ctcgaggcta gcgagctcgt
     6121 ttaaacaact agaattacac ggcgatcttg ccgcccttct tggccttaat gagaatctcg
     6181 cggatcttgc gggcgtccaa cttgccggtc agtcctttag gcacctcgtc cacgaacaca
     6241 acaccaccgc gcagcttctt ggcggttgta acctggctgg ccacatagtc cacgatctcc
     6301 ttctcggtca tggttttacc gtgttccagc acgacgactg cggcgggcag ctcgccggca
     6361 tcgtcgtcgg gcaggccggc gaccccggcg tcgaagatgt tggggtgttg cagcaggatg
     6421 ctctccagtt cggctggggc tacctggtag cccttgtatt tgatcaggct cttcagccgg
     6481 tccacgatga agaagtgctc gtcctcgtcc cagtaggcga tgtcgccgct gtgcagccag
     6541 ccgtccttgt cgatgagagc gtttgtagcc tcggggttgt taacgtagcc gctcatgatc
     6601 atggggccac ggacgcacag ctcgccgcgc tggttcacac ccagtgtctt accggtgtcc
     6661 aagtccacca ccttagcctc gaagaagggc accaccttgc ctactgcgcc aggcttgtcg
     6721 tccccttcgg gggtgatcag aatggcgctg gttgtttctg tcaggccgta gccctggcgg
     6781 atgcctggta ggtggaagcg tttggccacg gcctcaccta cctccttgct gagcggcgcc
     6841 ccgccgctgg cgatctcgtg caagttgctt aggtcgtact tgtcgatgag agtgctctta
     6901 gcgaagaagc taaatagtgt gggcaccagc agggcagatt gaatcttata gtcttgcaag
     6961 ctgcgcaaga atagctcctc ctcgaagcgg tacatgagca cgacccgaaa gccgcagatc
     7021 aagtagccca gcgtggtgaa catgccgaag ccgtggtgaa atggcaccac gctgaggata
     7081 gcggtgtcgg ggatgatctg gttgccgaag atggggtcgc gggcatgact gaatcggaca
     7141 caagcggtgc ggtgcggtag ggctacgccc ttgggcaatc cggtactgcc actactgttc
     7201 atgatcaggg cgatggtttt gtcccggtcg aagctctcgg gcacgaagtc gtactcgttg
     7261 aagccgggtg gcaaatggga agtcacgaag gtgtacatgc tttggaagcc ctggtagtcg
     7321 gtcttgctat ccatgatgat gatcttttgt atgatcggta gcttcttttg cacgttgagg
     7381 atcttttgca gccctttctt gctcacgaat acgacggtgg gctggctgat gcccatgctg
     7441 ttcagcagct cgcgctcgtt gtagatgtcg ttagctgggg ccacagccac accgatgaac
     7501 agggcaccca acacgggcat gaagaactgc aagctattct cgctgcacac cacgatccga
     7561 tggtttgtat tcagcccata gcgcttcata gcttctgcca gccgaacgct catctcgaag
     7621 tactcggcgt aggtaatgtc cacctcgata tgtgcgtcgg taaaggcgat ggtgccgggc
     7681 accagggcgt agcgcttcat ggctttgtgc agctgctcgc cggcggtccc gtcttcgagt
     7741 gggtagaatg gcgctgggcc cttcttaatg tttttggcat cttccatggt ggctttacca
     7801 acagtaccgg attgccaagc ttgggctgca ggtcgaaagg cccggagatg aggaagagga
     7861 gaacagcgcg gcagacgtgc gcttttgaag cgtgcagaat gccgggcctc cggaggacct
     7921 tcgggcgccc gccccgcccc tgagcccgcc cctgagcccg cccccggacc caccccttcc
     7981 cagcctctga gcccagaaag cgaaggagca aagctgctat tggccgctgc cccaaaggcc
     8041 tacccgcttc cattgctcag cggtgctgtc catctgcacg agactagtga gacgtgctac
     8101 ttccatttgt cacgtcctgc acgacgcgag ctgcggggcg ggggggaact tcctgactag
     8161 gggaggagta gaaggtggcg cgaaggggcc accaaagaac ggagccggtt ggcgcctacc
     8221 ggtggatgtg gaatgtgtgc gaggccagag gccacttgtg tagcgccaag tgcccagcgg
     8281 ggctgctaaa gcgcatgctc cagactgcct tgggaaaagc gcctccccta cccggtagat
     8341 ctcgatcctc tacgccggac gcatcgtggc cggagtactg cggtttatct cgatggctac
     8401 gagggcagac agtaagtgga tttaccataa tcccttaatt gtacgcaccg ctaaaacgcg
     8461 ttcagcgcga tcacggcagc agacaggtaa aaatggcaac aaaccacccg aaaaactgcc
     8521 gcaatcgcgc ctgataaatt ttaaccgtat gaatacctat gcaaccagag ggtacaggcc
     8581 acattacccc cacttaatcc actgaagctg ccatttttca tggtttcacc atcccagcga
     8641 agggccatcc agcgtgcgtt cctgtatttc cggactataa aaggatctag gtgaagatcc
     8701 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     8761 accccgtaga aaagatcaaa ggatcttc
//
