LOCUS       Exported               10056 bp ds-DNA     circular SYN 04-OCT-2024
DEFINITION  14_1.
ACCESSION   14_1
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10056)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Mar 28, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     source          1..10056
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      1..589
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(747..1607)
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.5"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(1608..1712)
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             1755..1847
                     /label=tnpA (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=96.8"
                     /note="/match_length=6.5"
                     /note="/other=CDS"
     polyA_signal    complement(1868..1916)
                     /label=poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     CDS             complement(1980..2772)
                     /label=NeoR/KanR (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.7"
                     /note="/match_length=99.7"
                     /note="/other=CDS"
     CDS             complement(2823..3755)
                     /label=hRluc
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=99.7"
                     /note="/other=CDS"
     promoter        complement(3806..4163)
                     /label=SV40 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    4228..4279
                     /label=bom (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=36.9"
                     /note="/other=misc_feature"
     rep_origin      complement(4280..4312)
                     /direction=LEFT
                     /label=M13 ori (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.0"
                     /note="/match_length=7.3"
                     /note="/other=rep_origin"
     CDS             4317..4385
                     /label=large T antigen (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.1"
                     /note="/match_length=3.2"
                     /note="/other=CDS"
     polyA_signal    4408..4529
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     terminator      complement(4551..4570)
                     /label=T7 terminator (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=41.7"
                     /note="/other=terminator"
     CDS             complement(4578..4627)
                     /label=SET1 (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=39.7"
                     /note="/other=CDS"
     misc_feature    complement(4634..4657)
                     /label=MCS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=42.1"
                     /note="/other=misc_feature"
     CDS             4675..4986
                     /label=GVQW1 (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=65.4"
                     /note="/match_length=53.3"
                     /note="/other=CDS"
     CDS             complement(7403..9055)
                     /label=luciferase
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(9102..9601)
                     /label=PGK promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    complement(9658..9941)
                     /label=cer region
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
ORIGIN
        1 ttgaaatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccggttg
      721 ggcgtcgctt ggtcggtcat ttcgaattac caatgcttaa tcagtgaggc acctatctca
      781 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
      841 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
      901 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
      961 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     1021 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     1081 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     1141 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat agttgtcaga
     1201 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     1261 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     1321 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     1381 ccacatagca gaactttgaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     1441 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     1501 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     1561 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     1621 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     1681 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     1741 gtctaagaaa ccattacgta gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat
     1801 gtcagctact gggctatctg gacaagggaa aacgcaagcg caaagaggat ccttatcgct
     1861 atcgattcac acaaaaaacc aacacacaga tgtaatgaaa ataaagatat tttattgcgg
     1921 ccatcgtgat ggcaggttgg gcgtcgcttg gtcggtcatt tcgaacccca gagtcccgct
     1981 cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata
     2041 ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aatatcacgg
     2101 gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca gtcgatgaat
     2161 ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc atgggtcacg
     2221 acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg
     2281 agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc catccgagta
     2341 cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc
     2401 gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg agcaaggtga
     2461 gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct tcccgcttca
     2521 gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca cgatagccgc
     2581 gctgcctcgt cctgcagttc attcagggca ccggacaggt cggtcttgac aaaaagaacc
     2641 gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat tgtctgttgt
     2701 gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc gtgcaatcca
     2761 tcttgttcaa tccctccgga gcctccgcca cctgatccgc cacctccgct cccaccaccg
     2821 gtctgctcgt tcttcagcac gcgctccacg aagctcttga tgtacttacc catttcatct
     2881 ggagcgtcct cctggctgaa gtggaggccc ttcaccttca cgaactcggt gttagggaac
     2941 ttcttagctc cctcgacaat agcgttggaa aagaacccag ggtcggactc gatgaacatc
     3001 ttaggcagat cgtcgctggc ccgaaggtag gcgttgtagt tgcggacaat ctggacgacg
     3061 tcgggcttgc ctcccttaac gagagggatc tcgcgaggcc aggagagggt aggccgtcta
     3121 acctcgccct tctccttgaa tggctccagg taggcagcga actcctcagg ctccagtttc
     3181 cgcatgatct tgcttgggag catggtctcg acgaagaagt tattctcaag caccattttc
     3241 tcgccctctt cgctcttgat cagggcgata tcctcctcga tgtcaggcca ctcgtcccag
     3301 gactcgatca cgtccacgac actctcagca tggacgatgg ccttgatctt gtcttggtgc
     3361 tcgtaggagt agtgaaaggc cagacaagcc ccccagtcgt ggcccacaaa gatgattttc
     3421 tttggaaggt tcagcagctc gaaccaagcg gtgaggtact tgtagtgatc caggaggcga
     3481 tatgagccat tcccgctctt gccggactta cccattccga tcagatcagg gatgatgcat
     3541 ctagccacgg gctcgatgtg aggcacgacg tgcctccaca ggtagctgga ggcagcgtta
     3601 ccatgcagaa aaatcacggc gttctcggcg tgcttctcgg aatcatagta gttgatgaag
     3661 gagtccagca cgttcatttg cttgcagcga gcccaccact gaggcccagt gatcatgcgt
     3721 ttgcgttgct cggggtcgta caccttggaa gccatggtgg ctccagcctt tggttcgaga
     3781 ctgttgtgtc agaagaatca agctttttgc aaaagcctag gcctccaaaa aagcctcctc
     3841 actacttctg gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa
     3901 ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg
     3961 gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc
     4021 ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt
     4081 gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc
     4141 acagctggtt ctttccgcct cagaaggtac ctaaccaagt tcctctttca gaggttattt
     4201 caggccatgg tgctgcgcag atccgcgtat gcggtgtgaa ataccgcaca gatgcgtaag
     4261 gagaaaatac cgcatcagga aattgtaagc gttaatattt tgttaaaatt cgattttacc
     4321 acatttgtag aggttttact tgcttaaaaa aacctcccac atctccccct gaacctgaaa
     4381 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     4441 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     4501 ggtttgtcca aactcatcaa tgtatcttat catgtctgct cgaagcggcc gccccaaggg
     4561 gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt cgggctttgt
     4621 tagcagccgg atcagcttgc atgcctgcag gtcgactatt tcgttttcag cattatttcc
     4681 tttttttttt tttttgagat ggagtctgtc tccattgccc aggctggagt gcaatggcgc
     4741 aatcttcgtt cactgcaacc tccgcctccc gggttcaagc gattctcctg ccttagcctc
     4801 ccaagtagtt gggattacag gcacccgcca ccatgcccag ctagtttttt tttttttttt
     4861 ttcctagtag agacagggtt tcaccatgtt ggccaggctg gtctcaaact cctgacctca
     4921 ggtgatcctc ccgcctcggc ctcccaaagt gctgggatta caggcgtgag ccaccgtgcc
     4981 tggcctattt ccagtttttt tttttaagat taataaaatg gagtcgccat tgctttgttc
     5041 aacatttccc tttgagtaat aacataatta atctactgaa aagaggagcc atgggggctt
     5101 ataaagaaat gccgtaatac atgtcagccc aacaacaaca aaatgtgcaa cagaacagcc
     5161 cagaaagaaa actgaaatgg gaacctctga taagaatttt tctttgcatg aggctcctgg
     5221 gcctacaact aaacaagtat cgcactgccc aagtagctgg aaggcatttc ctatttaaag
     5281 ggtgcaatca ctagaaagga gttcaggcca cagtgactgt cagcaagctc agattacagc
     5341 aaaacctctg aagttatttc caaaacataa tcagagctct cctctgcaca gtcagagcag
     5401 tggcaaattc ctagaactga tttttgttgc tgagttcagc actaaacaat ctctgagaaa
     5461 gctcagatta gccaggctga ataaacaaga aacagaccaa aaaaatttcc agtggagttt
     5521 cagccacaac taaatgagtt gtcctgtttt accaaaaatt catgcacata gctaagcact
     5581 ctcctcatct tcaaaaaaaa actatagttg tccaactttc taaaattctc ttaagtttga
     5641 taatgttaaa aacaatgata gtgagacctc cactattctt tccctcagat atacaaataa
     5701 tatatctaaa gcaagtacat ggtattttga taaaaactgt ctatagccac acaagctgta
     5761 ttatttctgc ccatgtttta aaaattaaga gtctttagtt gaaatccatc ctaattaccc
     5821 cagcccaaag tatagaagaa caaaaagtta cagggttctc taaaagcttt ttgttaagaa
     5881 acaagcccca ttttaaagaa aaatcttaaa ggatctatag gaatagatac aatttcagga
     5941 agaatttcat atcttcaagt ttatgtttaa gaagaaatac aactggttaa atcaaattta
     6001 ataacgtatt tattttaaga ataaaatgcc ctcttttgtt ggtgccagaa tttgtgttca
     6061 tactttgttg tcactagtgg tgcagattct cttctactaa aatttagttg agggtgtttg
     6121 aaagacctca aatgggcttc tctgggtatc gtttctcaag gctcccacag agtgaaaaac
     6181 caaacagtca cagaataaac tcttacagag gcagttattc atttcctgag ctaacccact
     6241 tccttcttca tacatttcct tctcttcatc aacgtgtttt ttgaaattag cattgaaatc
     6301 atcttgcaaa atatactttt ggacctaggg ccgggattct aggcaaattc caatggccat
     6361 gtcattttta catcatcact gtagtgatac aaaattagaa cgccaaatga agtgtcatgt
     6421 cagtagtaca gaaggctccc tgcaatgcta attgtgtttc agcaaaagaa tctgaatcat
     6481 tatgaactag atccatactg tgagaaaaca aatgttatta aaaaaactga atgcaaaaaa
     6541 tttcatggtt cctgactact ccatatggaa aagtatggca tctatcataa ggaaataaat
     6601 ttttttaacc tttcaaatct tagcgaaact gtaatgagtt gttgggtttc tccactctaa
     6661 atacaacctt ggagataaaa actggaagtc tcacaattat taaatttacc tattttatta
     6721 gacatgcacc ttctcaatga ttattgtcct gactttgatc aaagctaaca caagaagttg
     6781 aactaaaatg tggctctcat tattcttctg tgctaataaa tataaaatat aatttaaaaa
     6841 ataaaatcag ttcttaccta aatataaaaa gctttactta cttaacgaca atgagtgata
     6901 ccacagaaac ggacaaaaga aataaagtct ctcaaaatgt ttatttttgt cagaatagct
     6961 gacaattttc tgttaagtat tttggtttat tattataata agcatttccc ccccttgaaa
     7021 aatccattca aaatatctca accattgagg taaagcattt taactaaaaa aaaaaatcct
     7081 aatttgtttt taaagaaaaa aaaaatctta aaattccttt tgcttgataa atagaaataa
     7141 tatcttcttg ttctactttg gtgaacacaa atgtgccaag aatccagtca cttactggca
     7201 aaccaaaggt gggaacaatt catttacacg ttctactcaa acacagtgca acactgccgt
     7261 gttcatatat aattttcttt atagttacca acacttttct ggcaaagaca aagttttgga
     7321 atggcaaaag caacagaagt tttagatctc aaagctccag aaaggaaact cgaggctagc
     7381 gagctcgttt aaacaactag aattacacgg cgatcttgcc gcccttcttg gccttaatga
     7441 gaatctcgcg gatcttgcgg gcgtccaact tgccggtcag tcctttaggc acctcgtcca
     7501 cgaacacaac accaccgcgc agcttcttgg cggttgtaac ctggctggcc acatagtcca
     7561 cgatctcctt ctcggtcatg gttttaccgt gttccagcac gacgactgcg gcgggcagct
     7621 cgccggcatc gtcgtcgggc aggccggcga ccccggcgtc gaagatgttg gggtgttgca
     7681 gcaggatgct ctccagttcg gctggggcta cctggtagcc cttgtatttg atcaggctct
     7741 tcagccggtc cacgatgaag aagtgctcgt cctcgtccca gtaggcgatg tcgccgctgt
     7801 gcagccagcc gtccttgtcg atgagagcgt ttgtagcctc ggggttgtta acgtagccgc
     7861 tcatgatcat ggggccacgg acgcacagct cgccgcgctg gttcacaccc agtgtcttac
     7921 cggtgtccaa gtccaccacc ttagcctcga agaagggcac caccttgcct actgcgccag
     7981 gcttgtcgtc cccttcgggg gtgatcagaa tggcgctggt tgtttctgtc aggccgtagc
     8041 cctggcggat gcctggtagg tggaagcgtt tggccacggc ctcacctacc tccttgctga
     8101 gcggcgcccc gccgctggcg atctcgtgca agttgcttag gtcgtacttg tcgatgagag
     8161 tgctcttagc gaagaagcta aatagtgtgg gcaccagcag ggcagattga atcttatagt
     8221 cttgcaagct gcgcaagaat agctcctcct cgaagcggta catgagcacg acccgaaagc
     8281 cgcagatcaa gtagcccagc gtggtgaaca tgccgaagcc gtggtgaaat ggcaccacgc
     8341 tgaggatagc ggtgtcgggg atgatctggt tgccgaagat ggggtcgcgg gcatgactga
     8401 atcggacaca agcggtgcgg tgcggtaggg ctacgccctt gggcaatccg gtactgccac
     8461 tactgttcat gatcagggcg atggttttgt cccggtcgaa gctctcgggc acgaagtcgt
     8521 actcgttgaa gccgggtggc aaatgggaag tcacgaaggt gtacatgctt tggaagccct
     8581 ggtagtcggt cttgctatcc atgatgatga tcttttgtat gatcggtagc ttcttttgca
     8641 cgttgaggat cttttgcagc cctttcttgc tcacgaatac gacggtgggc tggctgatgc
     8701 ccatgctgtt cagcagctcg cgctcgttgt agatgtcgtt agctggggcc acagccacac
     8761 cgatgaacag ggcacccaac acgggcatga agaactgcaa gctattctcg ctgcacacca
     8821 cgatccgatg gtttgtattc agcccatagc gcttcatagc ttctgccagc cgaacgctca
     8881 tctcgaagta ctcggcgtag gtaatgtcca cctcgatatg tgcgtcggta aaggcgatgg
     8941 tgccgggcac cagggcgtag cgcttcatgg ctttgtgcag ctgctcgccg gcggtcccgt
     9001 cttcgagtgg gtagaatggc gctgggccct tcttaatgtt tttggcatct tccatggtgg
     9061 ctttaccaac agtaccggat tgccaagctt gggctgcagg tcgaaaggcc cggagatgag
     9121 gaagaggaga acagcgcggc agacgtgcgc ttttgaagcg tgcagaatgc cgggcctccg
     9181 gaggaccttc gggcgcccgc cccgcccctg agcccgcccc tgagcccgcc cccggaccca
     9241 ccccttccca gcctctgagc ccagaaagcg aaggagcaaa gctgctattg gccgctgccc
     9301 caaaggccta cccgcttcca ttgctcagcg gtgctgtcca tctgcacgag actagtgaga
     9361 cgtgctactt ccatttgtca cgtcctgcac gacgcgagct gcggggcggg ggggaacttc
     9421 ctgactaggg gaggagtaga aggtggcgcg aaggggccac caaagaacgg agccggttgg
     9481 cgcctaccgg tggatgtgga atgtgtgcga ggccagaggc cacttgtgta gcgccaagtg
     9541 cccagcgggg ctgctaaagc gcatgctcca gactgccttg ggaaaagcgc ctcccctacc
     9601 cggtagatct cgatcctcta cgccggacgc atcgtggccg gagtactgcg gtttatctcg
     9661 atggctacga gggcagacag taagtggatt taccataatc ccttaattgt acgcaccgct
     9721 aaaacgcgtt cagcgcgatc acggcagcag acaggtaaaa atggcaacaa accacccgaa
     9781 aaactgccgc aatcgcgcct gataaatttt aaccgtatga atacctatgc aaccagaggg
     9841 tacaggccac attaccccca cttaatccac tgaagctgcc atttttcatg gtttcaccat
     9901 cccagcgaag ggccatccag cgtgcgttcc tgtatttccg gactataaaa ggatctaggt
     9961 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
    10021 agcgtcagac cccgtagaaa agatcaaagg atcttc
//
