LOCUS       Exported                8888 bp ds-DNA     circular SYN 04-OCT-2024
DEFINITION  20_1.
ACCESSION   20_1
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Mar 28, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     source          1..8888
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      1..589
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(747..1607)
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.5"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(1608..1712)
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             1755..1847
                     /label=tnpA (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=96.8"
                     /note="/match_length=6.5"
                     /note="/other=CDS"
     polyA_signal    complement(1868..1916)
                     /label=poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     CDS             complement(1980..2772)
                     /label=NeoR/KanR (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.7"
                     /note="/match_length=99.7"
                     /note="/other=CDS"
     CDS             complement(2823..3755)
                     /label=hRluc
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=99.7"
                     /note="/other=CDS"
     promoter        complement(3806..4163)
                     /label=SV40 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    4228..4279
                     /label=bom (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=36.9"
                     /note="/other=misc_feature"
     rep_origin      complement(4280..4312)
                     /direction=LEFT
                     /label=M13 ori (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.0"
                     /note="/match_length=7.3"
                     /note="/other=rep_origin"
     CDS             4317..4385
                     /label=large T antigen (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.1"
                     /note="/match_length=3.2"
                     /note="/other=CDS"
     polyA_signal    4408..4529
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     terminator      complement(4551..4570)
                     /label=T7 terminator (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=41.7"
                     /note="/other=terminator"
     CDS             complement(4578..4627)
                     /label=SET1 (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=39.7"
                     /note="/other=CDS"
     misc_feature    complement(4634..4658)
                     /label=MCS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=43.9"
                     /note="/other=misc_feature"
     CDS             complement(5975..6160)
                     /label=C9orf85 (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=75.8"
                     /note="/match_length=34.6"
                     /note="/other=CDS"
     CDS             complement(6235..7887)
                     /label=luciferase
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(7934..8433)
                     /label=PGK promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    complement(8490..8773)
                     /label=cer region
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
ORIGIN
        1 ttgaaatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccggttg
      721 ggcgtcgctt ggtcggtcat ttcgaattac caatgcttaa tcagtgaggc acctatctca
      781 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
      841 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
      901 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
      961 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     1021 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     1081 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     1141 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat agttgtcaga
     1201 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     1261 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     1321 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     1381 ccacatagca gaactttgaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     1441 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     1501 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     1561 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     1621 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     1681 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     1741 gtctaagaaa ccattacgta gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat
     1801 gtcagctact gggctatctg gacaagggaa aacgcaagcg caaagaggat ccttatcgct
     1861 atcgattcac acaaaaaacc aacacacaga tgtaatgaaa ataaagatat tttattgcgg
     1921 ccatcgtgat ggcaggttgg gcgtcgcttg gtcggtcatt tcgaacccca gagtcccgct
     1981 cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata
     2041 ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aatatcacgg
     2101 gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca gtcgatgaat
     2161 ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc atgggtcacg
     2221 acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg
     2281 agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc catccgagta
     2341 cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc
     2401 gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg agcaaggtga
     2461 gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct tcccgcttca
     2521 gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca cgatagccgc
     2581 gctgcctcgt cctgcagttc attcagggca ccggacaggt cggtcttgac aaaaagaacc
     2641 gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat tgtctgttgt
     2701 gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc gtgcaatcca
     2761 tcttgttcaa tccctccgga gcctccgcca cctgatccgc cacctccgct cccaccaccg
     2821 gtctgctcgt tcttcagcac gcgctccacg aagctcttga tgtacttacc catttcatct
     2881 ggagcgtcct cctggctgaa gtggaggccc ttcaccttca cgaactcggt gttagggaac
     2941 ttcttagctc cctcgacaat agcgttggaa aagaacccag ggtcggactc gatgaacatc
     3001 ttaggcagat cgtcgctggc ccgaaggtag gcgttgtagt tgcggacaat ctggacgacg
     3061 tcgggcttgc ctcccttaac gagagggatc tcgcgaggcc aggagagggt aggccgtcta
     3121 acctcgccct tctccttgaa tggctccagg taggcagcga actcctcagg ctccagtttc
     3181 cgcatgatct tgcttgggag catggtctcg acgaagaagt tattctcaag caccattttc
     3241 tcgccctctt cgctcttgat cagggcgata tcctcctcga tgtcaggcca ctcgtcccag
     3301 gactcgatca cgtccacgac actctcagca tggacgatgg ccttgatctt gtcttggtgc
     3361 tcgtaggagt agtgaaaggc cagacaagcc ccccagtcgt ggcccacaaa gatgattttc
     3421 tttggaaggt tcagcagctc gaaccaagcg gtgaggtact tgtagtgatc caggaggcga
     3481 tatgagccat tcccgctctt gccggactta cccattccga tcagatcagg gatgatgcat
     3541 ctagccacgg gctcgatgtg aggcacgacg tgcctccaca ggtagctgga ggcagcgtta
     3601 ccatgcagaa aaatcacggc gttctcggcg tgcttctcgg aatcatagta gttgatgaag
     3661 gagtccagca cgttcatttg cttgcagcga gcccaccact gaggcccagt gatcatgcgt
     3721 ttgcgttgct cggggtcgta caccttggaa gccatggtgg ctccagcctt tggttcgaga
     3781 ctgttgtgtc agaagaatca agctttttgc aaaagcctag gcctccaaaa aagcctcctc
     3841 actacttctg gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa
     3901 ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg
     3961 gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc
     4021 ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt
     4081 gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc
     4141 acagctggtt ctttccgcct cagaaggtac ctaaccaagt tcctctttca gaggttattt
     4201 caggccatgg tgctgcgcag atccgcgtat gcggtgtgaa ataccgcaca gatgcgtaag
     4261 gagaaaatac cgcatcagga aattgtaagc gttaatattt tgttaaaatt cgattttacc
     4321 acatttgtag aggttttact tgcttaaaaa aacctcccac atctccccct gaacctgaaa
     4381 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     4441 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     4501 ggtttgtcca aactcatcaa tgtatcttat catgtctgct cgaagcggcc gccccaaggg
     4561 gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt cgggctttgt
     4621 tagcagccgg atcagcttgc atgcctgcag gtcgactcaa acaggcttcc ctttttctcc
     4681 tattgttgta ctcttatgtg tctgatatac acatttccta gtcttaactt tcaggagttt
     4741 acaattgact aacactccat gattgattca gtcatgaacc tcatcccatg tttcatctgt
     4801 ggacaattgc ttactttgtg ggttctttta aaagtaacac gaaatcatca tattgcataa
     4861 aaccttaaag ttctgttggt atcacagaag acaaggcaga gtttaaagtg aggaatttta
     4921 tatttaaaga actttttggt tggataaaaa cataatttga gcatccagtt ttagtatttc
     4981 actacatctc agttggtggg tgttaagcta gaatgggctg tgtgatagga aacaaatgcc
     5041 ttacagatgt gcctaggtgt tctgtttacc tagtgtctta ctctgttttc tggatctgaa
     5101 gactagtaat aaactaggac actaactggg ttccatgtga ttgccctttc atatgatctt
     5161 ctaagttgat ttttttcctc ccaagtcttt tttaaagaaa gtatactgta ttttaccaac
     5221 cccctctctt ttcttttagc tcctctgtgg tgaattaaac gtacttgagt taaaatattt
     5281 cgattttttt ttttaatgga aagtcctgca taacaacact gggccttctt aactaaaatg
     5341 ctcaccactt agcctgtttt tttatccctt ttttaaaatg acagatgatt ttgttcagga
     5401 attttgctgt ttttcttagt gctaatacct tgcctcttat tcctgctaca gcagggtggt
     5461 aatattggca ttctgattaa atactgtgcc ttaggagact ggaagtttaa aaatgtacaa
     5521 gtcctttcag tgatgaggga attgattttt tttaaaagtc tttttcttag aaagccaaaa
     5581 tgtttgtttt tttaagattc tgaaatgtgt tgtgacaaca atgacctatt tatgatctta
     5641 aatctttttt aaaaaaatgt ttgttttctg tgtggtgttt ttcgtattta aatccgaatg
     5701 tatgatgtgg cagtaacagg ttaacttatg taatttcttt agtacatagg gcttaggttt
     5761 atactcttgg tttccactca cactaatgtc acatggttaa gaaaaactta ggctctctga
     5821 agtttcagat atttcatgaa tcaagatgtg cttccttcaa agagagctct aaaatacttt
     5881 actgtggtta tgaagttatt atgggccagg catggtggct cacgccttca atcccagcac
     5941 tttgggaggc cgaggcaggc ggatcacctg aggctaggag ttcgagacca gcctggccaa
     6001 catggtgaaa ccccatccct actaaaatta ccaaaattag ccgggtgtgg tggtgcatgc
     6061 ctgtagtccc agctacttgg gaggctgagg caggaaaatc gcttgaacct gggaggcaga
     6121 ggttgcagtg agctgagatt gcaccactgc actctagctt gagtgacaga gtgactctgt
     6181 ctcaaaaaag aaaaaaatta ctcgaggcta gcgagctcgt ttaaacaact agaattacac
     6241 ggcgatcttg ccgcccttct tggccttaat gagaatctcg cggatcttgc gggcgtccaa
     6301 cttgccggtc agtcctttag gcacctcgtc cacgaacaca acaccaccgc gcagcttctt
     6361 ggcggttgta acctggctgg ccacatagtc cacgatctcc ttctcggtca tggttttacc
     6421 gtgttccagc acgacgactg cggcgggcag ctcgccggca tcgtcgtcgg gcaggccggc
     6481 gaccccggcg tcgaagatgt tggggtgttg cagcaggatg ctctccagtt cggctggggc
     6541 tacctggtag cccttgtatt tgatcaggct cttcagccgg tccacgatga agaagtgctc
     6601 gtcctcgtcc cagtaggcga tgtcgccgct gtgcagccag ccgtccttgt cgatgagagc
     6661 gtttgtagcc tcggggttgt taacgtagcc gctcatgatc atggggccac ggacgcacag
     6721 ctcgccgcgc tggttcacac ccagtgtctt accggtgtcc aagtccacca ccttagcctc
     6781 gaagaagggc accaccttgc ctactgcgcc aggcttgtcg tccccttcgg gggtgatcag
     6841 aatggcgctg gttgtttctg tcaggccgta gccctggcgg atgcctggta ggtggaagcg
     6901 tttggccacg gcctcaccta cctccttgct gagcggcgcc ccgccgctgg cgatctcgtg
     6961 caagttgctt aggtcgtact tgtcgatgag agtgctctta gcgaagaagc taaatagtgt
     7021 gggcaccagc agggcagatt gaatcttata gtcttgcaag ctgcgcaaga atagctcctc
     7081 ctcgaagcgg tacatgagca cgacccgaaa gccgcagatc aagtagccca gcgtggtgaa
     7141 catgccgaag ccgtggtgaa atggcaccac gctgaggata gcggtgtcgg ggatgatctg
     7201 gttgccgaag atggggtcgc gggcatgact gaatcggaca caagcggtgc ggtgcggtag
     7261 ggctacgccc ttgggcaatc cggtactgcc actactgttc atgatcaggg cgatggtttt
     7321 gtcccggtcg aagctctcgg gcacgaagtc gtactcgttg aagccgggtg gcaaatggga
     7381 agtcacgaag gtgtacatgc tttggaagcc ctggtagtcg gtcttgctat ccatgatgat
     7441 gatcttttgt atgatcggta gcttcttttg cacgttgagg atcttttgca gccctttctt
     7501 gctcacgaat acgacggtgg gctggctgat gcccatgctg ttcagcagct cgcgctcgtt
     7561 gtagatgtcg ttagctgggg ccacagccac accgatgaac agggcaccca acacgggcat
     7621 gaagaactgc aagctattct cgctgcacac cacgatccga tggtttgtat tcagcccata
     7681 gcgcttcata gcttctgcca gccgaacgct catctcgaag tactcggcgt aggtaatgtc
     7741 cacctcgata tgtgcgtcgg taaaggcgat ggtgccgggc accagggcgt agcgcttcat
     7801 ggctttgtgc agctgctcgc cggcggtccc gtcttcgagt gggtagaatg gcgctgggcc
     7861 cttcttaatg tttttggcat cttccatggt ggctttacca acagtaccgg attgccaagc
     7921 ttgggctgca ggtcgaaagg cccggagatg aggaagagga gaacagcgcg gcagacgtgc
     7981 gcttttgaag cgtgcagaat gccgggcctc cggaggacct tcgggcgccc gccccgcccc
     8041 tgagcccgcc cctgagcccg cccccggacc caccccttcc cagcctctga gcccagaaag
     8101 cgaaggagca aagctgctat tggccgctgc cccaaaggcc tacccgcttc cattgctcag
     8161 cggtgctgtc catctgcacg agactagtga gacgtgctac ttccatttgt cacgtcctgc
     8221 acgacgcgag ctgcggggcg ggggggaact tcctgactag gggaggagta gaaggtggcg
     8281 cgaaggggcc accaaagaac ggagccggtt ggcgcctacc ggtggatgtg gaatgtgtgc
     8341 gaggccagag gccacttgtg tagcgccaag tgcccagcgg ggctgctaaa gcgcatgctc
     8401 cagactgcct tgggaaaagc gcctccccta cccggtagat ctcgatcctc tacgccggac
     8461 gcatcgtggc cggagtactg cggtttatct cgatggctac gagggcagac agtaagtgga
     8521 tttaccataa tcccttaatt gtacgcaccg ctaaaacgcg ttcagcgcga tcacggcagc
     8581 agacaggtaa aaatggcaac aaaccacccg aaaaactgcc gcaatcgcgc ctgataaatt
     8641 ttaaccgtat gaatacctat gcaaccagag ggtacaggcc acattacccc cacttaatcc
     8701 actgaagctg ccatttttca tggtttcacc atcccagcga agggccatcc agcgtgcgtt
     8761 cctgtatttc cggactataa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     8821 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     8881 ggatcttc
//
