LOCUS       Exported                9952 bp ds-DNA     circular SYN 04-OCT-2024
DEFINITION  T35.
ACCESSION   T35
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Mar 28, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     source          1..9952
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      1..589
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(747..1607)
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.5"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(1608..1712)
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             1755..1847
                     /label=tnpA (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=96.8"
                     /note="/match_length=6.5"
                     /note="/other=CDS"
     polyA_signal    complement(1868..1916)
                     /label=poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     CDS             complement(1980..2772)
                     /label=NeoR/KanR (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.7"
                     /note="/match_length=99.7"
                     /note="/other=CDS"
     CDS             complement(2823..3755)
                     /label=hRluc
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=99.7"
                     /note="/other=CDS"
     promoter        complement(3806..4163)
                     /label=SV40 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    4228..4279
                     /label=bom (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=36.9"
                     /note="/other=misc_feature"
     rep_origin      complement(4280..4312)
                     /direction=LEFT
                     /label=M13 ori (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.0"
                     /note="/match_length=7.3"
                     /note="/other=rep_origin"
     CDS             4317..4385
                     /label=large T antigen (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.1"
                     /note="/match_length=3.2"
                     /note="/other=CDS"
     polyA_signal    4408..4529
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     terminator      complement(4551..4570)
                     /label=T7 terminator (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=41.7"
                     /note="/other=terminator"
     CDS             complement(4578..4627)
                     /label=SET1 (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=39.7"
                     /note="/other=CDS"
     misc_feature    complement(4634..4657)
                     /label=MCS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=42.1"
                     /note="/other=misc_feature"
     CDS             complement(7299..8951)
                     /label=luciferase
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(8998..9497)
                     /label=PGK promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    complement(9554..9837)
                     /label=cer region
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
ORIGIN
        1 ttgaaatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccggttg
      721 ggcgtcgctt ggtcggtcat ttcgaattac caatgcttaa tcagtgaggc acctatctca
      781 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
      841 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
      901 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
      961 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     1021 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     1081 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     1141 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat agttgtcaga
     1201 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     1261 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     1321 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     1381 ccacatagca gaactttgaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     1441 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     1501 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     1561 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     1621 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     1681 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     1741 gtctaagaaa ccattacgta gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat
     1801 gtcagctact gggctatctg gacaagggaa aacgcaagcg caaagaggat ccttatcgct
     1861 atcgattcac acaaaaaacc aacacacaga tgtaatgaaa ataaagatat tttattgcgg
     1921 ccatcgtgat ggcaggttgg gcgtcgcttg gtcggtcatt tcgaacccca gagtcccgct
     1981 cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata
     2041 ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aatatcacgg
     2101 gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca gtcgatgaat
     2161 ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc atgggtcacg
     2221 acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg
     2281 agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc catccgagta
     2341 cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc
     2401 gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg agcaaggtga
     2461 gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct tcccgcttca
     2521 gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca cgatagccgc
     2581 gctgcctcgt cctgcagttc attcagggca ccggacaggt cggtcttgac aaaaagaacc
     2641 gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat tgtctgttgt
     2701 gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc gtgcaatcca
     2761 tcttgttcaa tccctccgga gcctccgcca cctgatccgc cacctccgct cccaccaccg
     2821 gtctgctcgt tcttcagcac gcgctccacg aagctcttga tgtacttacc catttcatct
     2881 ggagcgtcct cctggctgaa gtggaggccc ttcaccttca cgaactcggt gttagggaac
     2941 ttcttagctc cctcgacaat agcgttggaa aagaacccag ggtcggactc gatgaacatc
     3001 ttaggcagat cgtcgctggc ccgaaggtag gcgttgtagt tgcggacaat ctggacgacg
     3061 tcgggcttgc ctcccttaac gagagggatc tcgcgaggcc aggagagggt aggccgtcta
     3121 acctcgccct tctccttgaa tggctccagg taggcagcga actcctcagg ctccagtttc
     3181 cgcatgatct tgcttgggag catggtctcg acgaagaagt tattctcaag caccattttc
     3241 tcgccctctt cgctcttgat cagggcgata tcctcctcga tgtcaggcca ctcgtcccag
     3301 gactcgatca cgtccacgac actctcagca tggacgatgg ccttgatctt gtcttggtgc
     3361 tcgtaggagt agtgaaaggc cagacaagcc ccccagtcgt ggcccacaaa gatgattttc
     3421 tttggaaggt tcagcagctc gaaccaagcg gtgaggtact tgtagtgatc caggaggcga
     3481 tatgagccat tcccgctctt gccggactta cccattccga tcagatcagg gatgatgcat
     3541 ctagccacgg gctcgatgtg aggcacgacg tgcctccaca ggtagctgga ggcagcgtta
     3601 ccatgcagaa aaatcacggc gttctcggcg tgcttctcgg aatcatagta gttgatgaag
     3661 gagtccagca cgttcatttg cttgcagcga gcccaccact gaggcccagt gatcatgcgt
     3721 ttgcgttgct cggggtcgta caccttggaa gccatggtgg ctccagcctt tggttcgaga
     3781 ctgttgtgtc agaagaatca agctttttgc aaaagcctag gcctccaaaa aagcctcctc
     3841 actacttctg gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa
     3901 ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg
     3961 gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc
     4021 ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt
     4081 gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc
     4141 acagctggtt ctttccgcct cagaaggtac ctaaccaagt tcctctttca gaggttattt
     4201 caggccatgg tgctgcgcag atccgcgtat gcggtgtgaa ataccgcaca gatgcgtaag
     4261 gagaaaatac cgcatcagga aattgtaagc gttaatattt tgttaaaatt cgattttacc
     4321 acatttgtag aggttttact tgcttaaaaa aacctcccac atctccccct gaacctgaaa
     4381 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     4441 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     4501 ggtttgtcca aactcatcaa tgtatcttat catgtctgct cgaagcggcc gccccaaggg
     4561 gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt cgggctttgt
     4621 tagcagccgg atcagcttgc atgcctgcag gtcgactaca agaggaacct gctgtcaagt
     4681 ccctgccatt ttgcctctcc tgtctcccac cccctgcccc agacccagga gcccccctga
     4741 ggctttgcct tgcctgcata tttgtttcgc tcttactcag tttgggaatt caaattgtcc
     4801 tgcagaggtt cattcccctg accctttccc cacattggta agagtagctg ggttttctaa
     4861 gccactctct ggaatctctt tgtgttaggg tctcgatttg aggacattca tttcttcagc
     4921 agcccattag caactgagag cccagggatg tcctacagga tagtttcata gtgacaggtg
     4981 gcacttggct aatagaatat ggctgatatt gtcattaatc attttgtacc ttgacatggg
     5041 ttgtctaata aaactcggac ccttcttgtg aaatcagtta aataagactt gtctcggtca
     5101 cctgtgccct gtccagactc gaggcagtgg taacactgca cagtgctatg tggcttctct
     5161 ttgaggattt ttgggttttg taactaaatt cttgctgccc tcatactttt tatgtattag
     5221 aatcatattc gtattgccct tttaaaacat tgggatcctc caaaggcctg ccccatgtat
     5281 ttaacagtaa tacaggaagc atggcaggca ccatgcaaac caaggatgga tggtgcagtc
     5341 cctgtgtcag tgggcggtgg tttcctgctg gcctggaatc actcatcacc tgattgattg
     5401 gctctgtggt cctgggcagg tgcctcatag gtgtgtggat atgatgacgt ttctttaaaa
     5461 tgtatgtatt taacaaatac ttaattgtat taaggtcatg taccaaggat ttgataaagt
     5521 ttaaataatt tactctctac ttttatccat tttatccatt ttaactcatg taatcctcat
     5581 gtgagtattc ctgtttaaca cttgagtaaa ctgaggcaca gagaacataa gttgcatgcc
     5641 atagtcacac actgtgaaag tgaaaagaga atgtgtgcaa aacacgtcac agtcctggtt
     5701 tctgagtaaa ggcaggctgt tatctttaga atcaagctat cacagggaga taggcaatgc
     5761 tgtgggtgtt ggaggaaggt gagagcctgt tgctaacaat ttcctggttt taaagctaag
     5821 gctgatttta ttgggaagat ctcacatgtg tgtggcccct gagagttccc agtgcctttt
     5881 atttgcagtc cttccatttg gacctcctag ctgccccatc aggtcatctc cagggctcag
     5941 aggggtgaga ccatttccca aggtcacaga accagctctc tagtcaccac cctgcctctc
     6001 cctctcaccc agagtcagta ccagttttat ggctttatta caaactgctg ggtccctccc
     6061 attttcaact tgattgatgg gatgtcatcc cttatcctgt ctgacatttg cctctggcct
     6121 ggttgctaga agtttgcccc aggggcaaga gttgaaattt ggcttcctga ggtgggcttt
     6181 gtggtttgcg tccctaaagt gagcccacta ctggttgctt gtccatggcc aacaccagaa
     6241 atcccctgag cactacctgg gtctcattcc aagaaggaag agggtcagga gacctgggga
     6301 gtctcatatt ccaagttctt ctttctttct gggagcagtg ggcagttcat ggtgttaggg
     6361 cactcacccc cacagactgg caaaccctgc aggacttccg tggctgaggc tgtgaccgga
     6421 ggccaggaat gccgttgggt ggattgtgag tgaatgggcc ctttgagctg ccctctagag
     6481 agcaaatcca gtttcctgga gctcctgaat gaatatctgt actggctcgc tcagatgcag
     6541 aagctccatt gaccatgagg ccttgtgaac atcagtggcc acaggcccag tgtgctgctt
     6601 ggcactgcac tagtttagga cctgcagcat gtaggtagcg tcctagtgtt tataatacaa
     6661 agctgctctg cacagctttt ctgattcttc ttgcaatctc ctgaggatta tctgccccat
     6721 ttttaaaacg aggtggaata cccaaggtca tgtagccagt gagtgctctg gaaagccaaa
     6781 gcagctcatc ccttcctggg gaccacactg ctctgctcca ccagaccaca ctatgaaata
     6841 ggaataagtg ctcctgttgc aggactgctg ggaaaacagg tggtgtggga cttaagtcac
     6901 cataattttg aagacttgca tgcagagggc tccaggaatt gtagacatta aggaatttca
     6961 ctttcagttc tacccactac ttaagtactt gtcatgtact cttagaggag gccagtaatg
     7021 atcagaacca ttttacttta aaattaataa tattgtatta gagaatatat taaatggtta
     7081 tattgggtta tgttaggata tatacttgaa tggaaataca tgtactatta gcaatcatat
     7141 ttcatttatc cctgtaatta gacaagaaag cataatatag ctctactcat gggtacacat
     7201 accagtgtat aagattttta gaagtttact ttttaaaaat aaaagcaaaa tgtaagatct
     7261 taaactcgag gctagcgagc tcgtttaaac aactagaatt acacggcgat cttgccgccc
     7321 ttcttggcct taatgagaat ctcgcggatc ttgcgggcgt ccaacttgcc ggtcagtcct
     7381 ttaggcacct cgtccacgaa cacaacacca ccgcgcagct tcttggcggt tgtaacctgg
     7441 ctggccacat agtccacgat ctccttctcg gtcatggttt taccgtgttc cagcacgacg
     7501 actgcggcgg gcagctcgcc ggcatcgtcg tcgggcaggc cggcgacccc ggcgtcgaag
     7561 atgttggggt gttgcagcag gatgctctcc agttcggctg gggctacctg gtagcccttg
     7621 tatttgatca ggctcttcag ccggtccacg atgaagaagt gctcgtcctc gtcccagtag
     7681 gcgatgtcgc cgctgtgcag ccagccgtcc ttgtcgatga gagcgtttgt agcctcgggg
     7741 ttgttaacgt agccgctcat gatcatgggg ccacggacgc acagctcgcc gcgctggttc
     7801 acacccagtg tcttaccggt gtccaagtcc accaccttag cctcgaagaa gggcaccacc
     7861 ttgcctactg cgccaggctt gtcgtcccct tcgggggtga tcagaatggc gctggttgtt
     7921 tctgtcaggc cgtagccctg gcggatgcct ggtaggtgga agcgtttggc cacggcctca
     7981 cctacctcct tgctgagcgg cgccccgccg ctggcgatct cgtgcaagtt gcttaggtcg
     8041 tacttgtcga tgagagtgct cttagcgaag aagctaaata gtgtgggcac cagcagggca
     8101 gattgaatct tatagtcttg caagctgcgc aagaatagct cctcctcgaa gcggtacatg
     8161 agcacgaccc gaaagccgca gatcaagtag cccagcgtgg tgaacatgcc gaagccgtgg
     8221 tgaaatggca ccacgctgag gatagcggtg tcggggatga tctggttgcc gaagatgggg
     8281 tcgcgggcat gactgaatcg gacacaagcg gtgcggtgcg gtagggctac gcccttgggc
     8341 aatccggtac tgccactact gttcatgatc agggcgatgg ttttgtcccg gtcgaagctc
     8401 tcgggcacga agtcgtactc gttgaagccg ggtggcaaat gggaagtcac gaaggtgtac
     8461 atgctttgga agccctggta gtcggtcttg ctatccatga tgatgatctt ttgtatgatc
     8521 ggtagcttct tttgcacgtt gaggatcttt tgcagccctt tcttgctcac gaatacgacg
     8581 gtgggctggc tgatgcccat gctgttcagc agctcgcgct cgttgtagat gtcgttagct
     8641 ggggccacag ccacaccgat gaacagggca cccaacacgg gcatgaagaa ctgcaagcta
     8701 ttctcgctgc acaccacgat ccgatggttt gtattcagcc catagcgctt catagcttct
     8761 gccagccgaa cgctcatctc gaagtactcg gcgtaggtaa tgtccacctc gatatgtgcg
     8821 tcggtaaagg cgatggtgcc gggcaccagg gcgtagcgct tcatggcttt gtgcagctgc
     8881 tcgccggcgg tcccgtcttc gagtgggtag aatggcgctg ggcccttctt aatgtttttg
     8941 gcatcttcca tggtggcttt accaacagta ccggattgcc aagcttgggc tgcaggtcga
     9001 aaggcccgga gatgaggaag aggagaacag cgcggcagac gtgcgctttt gaagcgtgca
     9061 gaatgccggg cctccggagg accttcgggc gcccgccccg cccctgagcc cgcccctgag
     9121 cccgcccccg gacccacccc ttcccagcct ctgagcccag aaagcgaagg agcaaagctg
     9181 ctattggccg ctgccccaaa ggcctacccg cttccattgc tcagcggtgc tgtccatctg
     9241 cacgagacta gtgagacgtg ctacttccat ttgtcacgtc ctgcacgacg cgagctgcgg
     9301 ggcggggggg aacttcctga ctaggggagg agtagaaggt ggcgcgaagg ggccaccaaa
     9361 gaacggagcc ggttggcgcc taccggtgga tgtggaatgt gtgcgaggcc agaggccact
     9421 tgtgtagcgc caagtgccca gcggggctgc taaagcgcat gctccagact gccttgggaa
     9481 aagcgcctcc cctacccggt agatctcgat cctctacgcc ggacgcatcg tggccggagt
     9541 actgcggttt atctcgatgg ctacgagggc agacagtaag tggatttacc ataatccctt
     9601 aattgtacgc accgctaaaa cgcgttcagc gcgatcacgg cagcagacag gtaaaaatgg
     9661 caacaaacca cccgaaaaac tgccgcaatc gcgcctgata aattttaacc gtatgaatac
     9721 ctatgcaacc agagggtaca ggccacatta cccccactta atccactgaa gctgccattt
     9781 ttcatggttt caccatccca gcgaagggcc atccagcgtg cgttcctgta tttccggact
     9841 ataaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     9901 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tc
//
