LOCUS       Exported                7990 bp ds-DNA     circular SYN 04-OCT-2024
DEFINITION  T24.
ACCESSION   T24
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Mar 28, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     source          1..7990
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      1..589
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(747..1607)
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.5"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(1608..1712)
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             1755..1847
                     /label=tnpA (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=96.8"
                     /note="/match_length=6.5"
                     /note="/other=CDS"
     polyA_signal    complement(1868..1916)
                     /label=poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     CDS             complement(1980..2772)
                     /label=NeoR/KanR (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.7"
                     /note="/match_length=99.7"
                     /note="/other=CDS"
     CDS             complement(2823..3755)
                     /label=hRluc
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=99.7"
                     /note="/other=CDS"
     promoter        complement(3806..4163)
                     /label=SV40 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    4228..4279
                     /label=bom (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=36.9"
                     /note="/other=misc_feature"
     rep_origin      complement(4280..4312)
                     /direction=LEFT
                     /label=M13 ori (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.0"
                     /note="/match_length=7.3"
                     /note="/other=rep_origin"
     CDS             4317..4385
                     /label=large T antigen (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.1"
                     /note="/match_length=3.2"
                     /note="/other=CDS"
     polyA_signal    4408..4529
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     terminator      complement(4551..4570)
                     /label=T7 terminator (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=41.7"
                     /note="/other=terminator"
     CDS             complement(4578..4627)
                     /label=SET1 (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=39.7"
                     /note="/other=CDS"
     misc_feature    complement(4634..4657)
                     /label=MCS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=42.1"
                     /note="/other=misc_feature"
     CDS             complement(5337..6989)
                     /label=luciferase
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(7036..7535)
                     /label=PGK promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    complement(7592..7875)
                     /label=cer region
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
ORIGIN
        1 ttgaaatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccggttg
      721 ggcgtcgctt ggtcggtcat ttcgaattac caatgcttaa tcagtgaggc acctatctca
      781 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
      841 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
      901 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
      961 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     1021 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     1081 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     1141 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat agttgtcaga
     1201 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     1261 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     1321 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     1381 ccacatagca gaactttgaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     1441 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     1501 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     1561 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     1621 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     1681 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     1741 gtctaagaaa ccattacgta gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat
     1801 gtcagctact gggctatctg gacaagggaa aacgcaagcg caaagaggat ccttatcgct
     1861 atcgattcac acaaaaaacc aacacacaga tgtaatgaaa ataaagatat tttattgcgg
     1921 ccatcgtgat ggcaggttgg gcgtcgcttg gtcggtcatt tcgaacccca gagtcccgct
     1981 cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata
     2041 ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aatatcacgg
     2101 gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca gtcgatgaat
     2161 ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc atgggtcacg
     2221 acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg
     2281 agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc catccgagta
     2341 cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc
     2401 gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg agcaaggtga
     2461 gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct tcccgcttca
     2521 gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca cgatagccgc
     2581 gctgcctcgt cctgcagttc attcagggca ccggacaggt cggtcttgac aaaaagaacc
     2641 gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat tgtctgttgt
     2701 gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc gtgcaatcca
     2761 tcttgttcaa tccctccgga gcctccgcca cctgatccgc cacctccgct cccaccaccg
     2821 gtctgctcgt tcttcagcac gcgctccacg aagctcttga tgtacttacc catttcatct
     2881 ggagcgtcct cctggctgaa gtggaggccc ttcaccttca cgaactcggt gttagggaac
     2941 ttcttagctc cctcgacaat agcgttggaa aagaacccag ggtcggactc gatgaacatc
     3001 ttaggcagat cgtcgctggc ccgaaggtag gcgttgtagt tgcggacaat ctggacgacg
     3061 tcgggcttgc ctcccttaac gagagggatc tcgcgaggcc aggagagggt aggccgtcta
     3121 acctcgccct tctccttgaa tggctccagg taggcagcga actcctcagg ctccagtttc
     3181 cgcatgatct tgcttgggag catggtctcg acgaagaagt tattctcaag caccattttc
     3241 tcgccctctt cgctcttgat cagggcgata tcctcctcga tgtcaggcca ctcgtcccag
     3301 gactcgatca cgtccacgac actctcagca tggacgatgg ccttgatctt gtcttggtgc
     3361 tcgtaggagt agtgaaaggc cagacaagcc ccccagtcgt ggcccacaaa gatgattttc
     3421 tttggaaggt tcagcagctc gaaccaagcg gtgaggtact tgtagtgatc caggaggcga
     3481 tatgagccat tcccgctctt gccggactta cccattccga tcagatcagg gatgatgcat
     3541 ctagccacgg gctcgatgtg aggcacgacg tgcctccaca ggtagctgga ggcagcgtta
     3601 ccatgcagaa aaatcacggc gttctcggcg tgcttctcgg aatcatagta gttgatgaag
     3661 gagtccagca cgttcatttg cttgcagcga gcccaccact gaggcccagt gatcatgcgt
     3721 ttgcgttgct cggggtcgta caccttggaa gccatggtgg ctccagcctt tggttcgaga
     3781 ctgttgtgtc agaagaatca agctttttgc aaaagcctag gcctccaaaa aagcctcctc
     3841 actacttctg gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa
     3901 ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg
     3961 gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc
     4021 ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt
     4081 gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc
     4141 acagctggtt ctttccgcct cagaaggtac ctaaccaagt tcctctttca gaggttattt
     4201 caggccatgg tgctgcgcag atccgcgtat gcggtgtgaa ataccgcaca gatgcgtaag
     4261 gagaaaatac cgcatcagga aattgtaagc gttaatattt tgttaaaatt cgattttacc
     4321 acatttgtag aggttttact tgcttaaaaa aacctcccac atctccccct gaacctgaaa
     4381 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     4441 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     4501 ggtttgtcca aactcatcaa tgtatcttat catgtctgct cgaagcggcc gccccaaggg
     4561 gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt cgggctttgt
     4621 tagcagccgg atcagcttgc atgcctgcag gtcgactaca catttgttac ttttacatga
     4681 tctttattat ttaagaaaaa cctcttttaa ccatttatat aacagaaaaa aaatagggag
     4741 gctggtagat catcacatat atagtagcta aaatatgaaa ggccagggaa tttattatta
     4801 atgaagtcat aaaacagact taaccaaaag tgtgtgctag gaaacaagca gtttcacttc
     4861 agagacttca ttgcaggaac ccagtttcct tatgtggaaa aaagtgatta taaataacag
     4921 ttatctgaaa ggtggttgag aggattaaat gagatcacct atgcaaacaa atacatgtag
     4981 gtatgaagac atcgtctggg gtgtgaaagt ttagttcaca ccagaacctt cctttaaggc
     5041 ttaaaaacct caaacagtat ttcattctta atacaagaac caatgtttaa agcataaaat
     5101 ccttaattgg agtgttcagc ccagttgatt atcaaaagcg atttataaaa tgcttcaata
     5161 ctgatgatgt taaagcaacc aaaactttgt gatgctcaca gaggtttggc ttccggggtt
     5221 atggtccaaa gtcatttgcc ttctgagaag attgcagaga tgtcagatac ttaatttctc
     5281 aaacaacttg gcatcagcct tactcgaggc tagcgagctc gtttaaacaa ctagaattac
     5341 acggcgatct tgccgccctt cttggcctta atgagaatct cgcggatctt gcgggcgtcc
     5401 aacttgccgg tcagtccttt aggcacctcg tccacgaaca caacaccacc gcgcagcttc
     5461 ttggcggttg taacctggct ggccacatag tccacgatct ccttctcggt catggtttta
     5521 ccgtgttcca gcacgacgac tgcggcgggc agctcgccgg catcgtcgtc gggcaggccg
     5581 gcgaccccgg cgtcgaagat gttggggtgt tgcagcagga tgctctccag ttcggctggg
     5641 gctacctggt agcccttgta tttgatcagg ctcttcagcc ggtccacgat gaagaagtgc
     5701 tcgtcctcgt cccagtaggc gatgtcgccg ctgtgcagcc agccgtcctt gtcgatgaga
     5761 gcgtttgtag cctcggggtt gttaacgtag ccgctcatga tcatggggcc acggacgcac
     5821 agctcgccgc gctggttcac acccagtgtc ttaccggtgt ccaagtccac caccttagcc
     5881 tcgaagaagg gcaccacctt gcctactgcg ccaggcttgt cgtccccttc gggggtgatc
     5941 agaatggcgc tggttgtttc tgtcaggccg tagccctggc ggatgcctgg taggtggaag
     6001 cgtttggcca cggcctcacc tacctccttg ctgagcggcg ccccgccgct ggcgatctcg
     6061 tgcaagttgc ttaggtcgta cttgtcgatg agagtgctct tagcgaagaa gctaaatagt
     6121 gtgggcacca gcagggcaga ttgaatctta tagtcttgca agctgcgcaa gaatagctcc
     6181 tcctcgaagc ggtacatgag cacgacccga aagccgcaga tcaagtagcc cagcgtggtg
     6241 aacatgccga agccgtggtg aaatggcacc acgctgagga tagcggtgtc ggggatgatc
     6301 tggttgccga agatggggtc gcgggcatga ctgaatcgga cacaagcggt gcggtgcggt
     6361 agggctacgc ccttgggcaa tccggtactg ccactactgt tcatgatcag ggcgatggtt
     6421 ttgtcccggt cgaagctctc gggcacgaag tcgtactcgt tgaagccggg tggcaaatgg
     6481 gaagtcacga aggtgtacat gctttggaag ccctggtagt cggtcttgct atccatgatg
     6541 atgatctttt gtatgatcgg tagcttcttt tgcacgttga ggatcttttg cagccctttc
     6601 ttgctcacga atacgacggt gggctggctg atgcccatgc tgttcagcag ctcgcgctcg
     6661 ttgtagatgt cgttagctgg ggccacagcc acaccgatga acagggcacc caacacgggc
     6721 atgaagaact gcaagctatt ctcgctgcac accacgatcc gatggtttgt attcagccca
     6781 tagcgcttca tagcttctgc cagccgaacg ctcatctcga agtactcggc gtaggtaatg
     6841 tccacctcga tatgtgcgtc ggtaaaggcg atggtgccgg gcaccagggc gtagcgcttc
     6901 atggctttgt gcagctgctc gccggcggtc ccgtcttcga gtgggtagaa tggcgctggg
     6961 cccttcttaa tgtttttggc atcttccatg gtggctttac caacagtacc ggattgccaa
     7021 gcttgggctg caggtcgaaa ggcccggaga tgaggaagag gagaacagcg cggcagacgt
     7081 gcgcttttga agcgtgcaga atgccgggcc tccggaggac cttcgggcgc ccgccccgcc
     7141 cctgagcccg cccctgagcc cgcccccgga cccacccctt cccagcctct gagcccagaa
     7201 agcgaaggag caaagctgct attggccgct gccccaaagg cctacccgct tccattgctc
     7261 agcggtgctg tccatctgca cgagactagt gagacgtgct acttccattt gtcacgtcct
     7321 gcacgacgcg agctgcgggg cgggggggaa cttcctgact aggggaggag tagaaggtgg
     7381 cgcgaagggg ccaccaaaga acggagccgg ttggcgccta ccggtggatg tggaatgtgt
     7441 gcgaggccag aggccacttg tgtagcgcca agtgcccagc ggggctgcta aagcgcatgc
     7501 tccagactgc cttgggaaaa gcgcctcccc tacccggtag atctcgatcc tctacgccgg
     7561 acgcatcgtg gccggagtac tgcggtttat ctcgatggct acgagggcag acagtaagtg
     7621 gatttaccat aatcccttaa ttgtacgcac cgctaaaacg cgttcagcgc gatcacggca
     7681 gcagacaggt aaaaatggca acaaaccacc cgaaaaactg ccgcaatcgc gcctgataaa
     7741 ttttaaccgt atgaatacct atgcaaccag agggtacagg ccacattacc cccacttaat
     7801 ccactgaagc tgccattttt catggtttca ccatcccagc gaagggccat ccagcgtgcg
     7861 ttcctgtatt tccggactat aaaaggatct aggtgaagat cctttttgat aatctcatga
     7921 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     7981 aaggatcttc
//
