LOCUS       pelm41003_ptufaE        7198 bp ds-DNA     linear       13-DEC-2023
DEFINITION  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     terminator      5..99
                     /note="note: transcription terminator from phage lambda"
                     /locus_tag="lambda t0 terminator"
                     /label="lambda t0 terminator"
                     /ApEinfo_label="lambda t0 terminator"
                     /ApEinfo_fwdcolor="#d59687"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(6471..6506)
                     /locus_tag="tuf CDS (translation elongation factor Tu)"
                     /label="tuf CDS (translation elongation factor Tu)"
                     /ApEinfo_label="tuf CDS (translation elongation factor
                     Tu)"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(198..217)
                     /note="note: pENTR vectors, reverse primer"
                     /locus_tag="pENTR-R"
                     /label="pENTR-R"
                     /ApEinfo_label="pENTR-R"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     gene            complement(6471..6506)
                     /locus_tag="tuf gene"
                     /label="tuf gene"
                     /ApEinfo_label="tuf gene"
                     /ApEinfo_fwdcolor="#d6b295"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             209..1024
                     /note="product: aminoglycoside phosphotransferase note:
                     confers resistance to kanamycin in bacteria or G418
                     (Geneticin(R)) in eukaryotes translation:
                     MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGK
                     GSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDS
                     GENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWP
                     VEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAIL
                     WNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
                     /locus_tag="KanR"
                     /label="KanR"
                     /ApEinfo_label="KanR"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     source          complement(6471..6506)
                     /locus_tag="pathovar: tomato"
                     /label="pathovar: tomato"
                     /ApEinfo_label="pathovar: tomato"
                     /ApEinfo_fwdcolor="#b4abac"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1040..2585
                     /locus_tag="Position 3 module for suicide plasmid"
                     /label="Position 3 module for suicide plasmid"
                     /ApEinfo_label="Position 3 module for suicide plasmid"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer          complement(6493..6506)
                     /note="sequence: ttggtctcaACATggctaaagaaaaatttgatcg"
                     /locus_tag="tuf_DC3000-Nt-BsaI-ATGT-R"
                     /label="tuf_DC3000-Nt-BsaI-ATGT-R"
                     /ApEinfo_label="tuf_DC3000-Nt-BsaI-ATGT-R"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(1338..1883)
                     /note="direction: LEFT note: Plasmids containing the
                     medium-copy-number p15A origin of replication can be
                     propagated in E. coli cells that contain a second plasmid
                     with the ColE1 origin."
                     /locus_tag="p15A ori"
                     /label="p15A ori"
                     /ApEinfo_label="p15A ori"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(1697..2001)
                     /locus_tag="TRAJ"
                     /label="TRAJ"
                     /ApEinfo_label="TRAJ"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     oriT            2257..2366
                     /note="note: incP origin of transfer"
                     /locus_tag="oriT"
                     /label="oriT"
                     /ApEinfo_label="oriT"
                     /ApEinfo_fwdcolor="#b4abac"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2590..2741
                     /locus_tag="Position 3 module for suicide plasmid(1)"
                     /label="Position 3 module for suicide plasmid(1)"
                     /ApEinfo_label="Position 3 module for suicide plasmid"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        2746..3191
                     /note="gene: sacR note: sacB promoter and control region"
                     /locus_tag="sacB promoter"
                     /label="sacB promoter"
                     /ApEinfo_label="sacB promoter"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2746..4705
                     /locus_tag="Position 4 module for suicide plasmid"
                     /label="Position 4 module for suicide plasmid"
                     /ApEinfo_label="Position 4 module for suicide plasmid"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             3192..3278
                     /note="codon_start: 1 gene: Bacillus subtilis sacB note:
                     negative selection marker product: secreted levansucrase
                     that renders bacterial growth sensitive to sucrose
                     protein_id: transl_table: 1 translation:
                     MNIKKFAKQATVLTFTTALLAGGATQAFA,KETNQKPYKETYGISHITRHDMLQIPEQ
                     QKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYRGYHIVFALAGDPKN
                     ADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEWSGSATFTSDG
                     KIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDGKTYQNVQQF
                     IDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKAYYGKSTSF
                     FRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDEIERANVF
                     KMNGKWYLFTDSRGSKMTIDGISSNDIYMLGYVSNSLTGPYKPLNKTGLVLKMDLDPN
                     DVTFTYSHFAVPQAKGNNVVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSVVKDS
                     ILEQGQLTVNK*"
                     /locus_tag="SacB"
                     /label="SacB"
                     /ApEinfo_label="SacB"
                     /ApEinfo_fwdcolor="#993366"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             3279..4613
                     /note="codon_start: 1 gene: Bacillus subtilis sacB note:
                     negative selection marker product: secreted levansucrase
                     that renders bacterial growth sensitive to sucrose
                     protein_id: transl_table: 1 translation:
                     MNIKKFAKQATVLTFTTALLAGGATQAFA,KETNQKPYKETYGISHITRHDMLQIPEQ
                     QKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYRGYHIVFALAGDPKN
                     ADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEWSGSATFTSDG
                     KIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDGKTYQNVQQF
                     IDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKAYYGKSTSF
                     FRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDEIERANVF
                     KMNGKWYLFTDSRGSKMTIDGISSNDIYMLGYVSNSLTGPYKPLNKTGLVLKMDLDPN
                     DVTFTYSHFAVPQAKGNNVVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSVVKDS
                     ILEQGQLTVNK*"
                     /locus_tag="SacB(1)"
                     /label="SacB(1)"
                     /ApEinfo_label="SacB"
                     /ApEinfo_fwdcolor="#993366"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      4723..4809
                     /note="gene: Escherichia coli rrnB note: transcription
                     terminator T1 from the E. coli rrnB gene"
                     /locus_tag="rrnB T1 terminator"
                     /label="rrnB T1 terminator"
                     /ApEinfo_label="rrnB T1 terminator"
                     /ApEinfo_fwdcolor="#d59687"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer          4831..4851
                     /note="sequence: ttggtctcaGGAGcacgatcttgcggatgc"
                     /locus_tag="GV3101_tufA_UF-BsaI-GGAG"
                     /label="GV3101_tufA_UF-BsaI-GGAG"
                     /ApEinfo_label="GV3101_tufA_UF-BsaI-GGAG"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(4835..5197)
                     /locus_tag="galE CDS (UDP-glucose 4-epimerase)"
                     /label="galE CDS (UDP-glucose 4-epimerase)"
                     /ApEinfo_label="galE CDS (UDP-glucose 4-epimerase)"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     gene            complement(4835..5197)
                     /locus_tag="galE gene"
                     /label="galE gene"
                     /ApEinfo_label="galE gene"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     source          4835..5339
                     /locus_tag="source"
                     /label="source"
                     /ApEinfo_label="source"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer          complement(5322..5339)
                     /note="sequence: ttggtctcaAATTttcaggcctgaaaagg"
                     /locus_tag="GV3101_tufA_UR-DC3000tuf-ct-AATT"
                     /label="GV3101_tufA_UR-DC3000tuf-ct-AATT"
                     /ApEinfo_label="GV3101_tufA_UR-DC3000tuf-ct-AATT"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer          5337..5339
                     /note="sequence: ttggtctcaAATTacgcgatgattttggct"
                     /locus_tag="tuf-ct-DC3000-F-AATT"
                     /label="tuf-ct-DC3000-F-AATT"
                     /ApEinfo_label="tuf-ct-DC3000-F-AATT"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5338..5339
                     /locus_tag="fusion1"
                     /label="fusion1"
                     /ApEinfo_label="fusion1"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer          complement(6516..6517)
                     /note="sequence: ttggtctcaACATggctaaagaaaaatttgatcg"
                     /locus_tag="tuf_DC3000-Nt-BsaI-ATGT-R(1)"
                     /label="tuf_DC3000-Nt-BsaI-ATGT-R(1)"
                     /ApEinfo_label="tuf_DC3000-Nt-BsaI-ATGT-R"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6516..6517
                     /locus_tag="fusion3"
                     /label="fusion3"
                     /ApEinfo_label="fusion3"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer          6516..6535
                     /note="sequence: TTGGTCTCAATGTGAATTGCTTCCTGTGAAC"
                     /locus_tag="GV3101_tufA_DF-DC3000tuf-nt-ATGT"
                     /label="GV3101_tufA_DF-DC3000tuf-nt-ATGT"
                     /ApEinfo_label="GV3101_tufA_DF-DC3000tuf-nt-ATGT"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6516..7194
                     /locus_tag="Atu1966 downstream "
                     /label="Atu1966 downstream "
                     /ApEinfo_label="Atu1966 downstream "
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer          complement(join(7176..7198,1..1))
                     /note="sequence: aaggtctcgAGCGcagttccgtccacatcttc"
                     /locus_tag="GV3101_tufA_DR-CGCT"
                     /label="GV3101_tufA_DR-CGCT"
                     /ApEinfo_label="GV3101_tufA_DR-CGCT"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(5340..6515)
                     /locus_tag="Atu1966 (TufA)"
                     /label="Atu1966 (TufA)"
                     /ApEinfo_label="Atu1966 (TufA)"
                     /ApEinfo_fwdcolor="#ff8040"
                     /ApEinfo_revcolor="#ff8040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(6471..6506)
                     /locus_tag="EKFDRSLPHCNV (EF-TuDC3000)"
                     /label="EKFDRSLPHCNV (EF-TuDC3000)"
                     /ApEinfo_label="EKFDRSLPHCNV (EF-TuDC3000)"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 cttggactcc tgttgataga tccagtaatg acctcagaac tccatctgga tttgttcaga
       61 acgctcggtt gccgccgggc gttttttatt ggtgagaatc caggcaattg tgtctcaaaa
      121 tctctgatgt tacattgcac aagataaaaa tatatcatca tgaacaataa aactgtctgc
      181 ttacataaac agtaatacaa ggggtgttat gagccatatt cagcgtgaaa cgagctgtag
      241 ccgtccgcgt ctgaacagca acatggatgc ggatctgtat ggctataaat gggcgcgtga
      301 taacgtgggt cagagcggcg cgaccattta tcgtctgtat ggcaaaccgg atgcgccgga
      361 actgtttctg aaacatggca aaggcagcgt ggcgaacgat gtgaccgatg aaatggtgcg
      421 tctgaactgg ctgaccgaat ttatgccgct gccgaccatt aaacatttta ttcgcacccc
      481 ggatgatgcg tggctgctga ccaccgcgat tccgggcaaa accgcgtttc aggtgctgga
      541 agaatatccg gatagcggcg aaaacattgt ggatgcgctg gccgtgtttc tgcgtcgtct
      601 gcatagcatt ccggtgtgca actgcccgtt taacagcgat cgtgtgtttc gtctggccca
      661 ggcgcagagc cgtatgaaca acggcctggt ggatgcgagc gattttgatg atgaacgtaa
      721 cggctggccg gtggaacagg tgtggaaaga aatgcataaa ctgctgccgt ttagcccgga
      781 tagcgtggtg acccacggcg attttagcct ggataacctg attttcgatg aaggcaaact
      841 gattggctgc attgatgtgg gccgtgtggg cattgcggat cgttatcagg atctggccat
      901 tctgtggaac tgcctgggcg aatttagccc gagcctgcaa aaacgtctgt ttcagaaata
      961 tggcattgat aatccggata tgaacaaact gcaatttcat ctgatgctgg atgaattttt
     1021 ctaataatta attgaactat gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg
     1081 agtgtatact ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg
     1141 caggagaaaa aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc
     1201 ttcctcgctc actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta
     1261 cgaacggggc ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg
     1321 gccgcggcaa agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga
     1381 cgctcaaatc agtggtggcg aaacccgaca ggactataaa gataccaggc gtttCcccct
     1441 ggcggctccc tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg
     1501 ttatggccgc gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc
     1561 caagctggac tgtatgcacg aaccccccgt tcagtCcgac cgctgcgcct tatccggtaa
     1621 ctatcgtctt gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg
     1681 taattgattt agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga
     1741 caagttttgg tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc
     1801 agagaacctt cgAaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta
     1861 cgcgcagacc aaaacgatct caagaagatc atcttattaa tcagataaaa tatttctagc
     1921 tagagtcgat cttcgccagc agggcGagga tcgtggcatc accgaaccgc gccgtGCgcG
     1981 gGtcgtcggt gagccagagt ttcagcaggc cgcccaggtc gccattgatg cgggccagct
     2041 cgcggacgtg ctcatagtcc acgacgcccg tgattttgta gccctggccg acgGCcAGca
     2101 ggtaggccga caGgctcatg ccggccgccg ccgccttttc ctcaatcgct cttcgttcgt
     2161 ctggaaggca gtacaccttg ataggtgggc tgcccttcct ggttggcttg gtttcatcag
     2221 ccatccgctt gccctcatct gttacgccgg cggtagccgg ccagcctcgc agagcaggat
     2281 tcccgttgag caccgccagg tgcgaataag ggacagtgaa gaaggaacac ccgctcgcgg
     2341 gtgggcctac ttcacctatc ctgcccggct gacgccgttg gatacaccaa ggaaagtcta
     2401 cAcgaaccct ttggcaaaat cctgtatatc gtgcgaaaaa ggatggatat accgaaaaaa
     2461 tcgctataat gaccccgaag cagggttatg cagcggaaaa gcgctgcttc cctgctgttt
     2521 tgtggaatat ctaccgactg gaaacaggca aatgcaggaa attactgaac tgaggggaca
     2581 ggcgaGACAc gatgccaaag agctacaccg acgagctggc cgagtgggtt gaatcccgcg
     2641 cggccaagaa gcgccggcgt gatgaggctg cggttgcgtt cctggcggtg agggcggatg
     2701 tcgatcgact ctagctagag gatcgatcct ttttaaccca tTTACcacat atacctgccg
     2761 ttcactatta tttagtgaaa tgagatatta tgatattttc tgaattgtga ttaaaaaggc
     2821 aactttatgc ccatgcaaca gaaactataa aaaatacaga gaatgaaaag aaacagatag
     2881 attttttagt tctttaggcc cgtagtctgc aaatcctttt atgattttct atcaaacaaa
     2941 agaggaaaat agaccagttg caatccaaac gagagtctaa tagaatgagg tcgaaaagta
     3001 aatcgcgcgg gtttgttact gataaagcag gcaagaccta aaatgtgtaa agggcaaagt
     3061 gtatactttg gcgtcacccc ttacatattt taggtctttt tttattgtgc gtaactaact
     3121 tgccatcttc aaacaggagg gctggaagaa gcagaccgct aacacagtac ataaaaaagg
     3181 agacatgaac gatgaacatc aaaaagtttg caaaacaagc aacagtatta acctttacta
     3241 ccgcactgct ggcaggaggc gcaactcaag cgtttgcgaa agaaacgaac caaaagccat
     3301 ataaggaaac atacggcatt tcccatatta cacgccatga tatgctgcaa atccctgaac
     3361 agcaaaaaaa tgaaaaatat caagttcctg aattcgattc gtccacaatt aaaaatatct
     3421 cttctgcaaa aggcctggac gtttgggaca gctggccatt acaaaacgct gacggcactg
     3481 tcgcaaacta tcacggctac cacatcgtct ttgcattagc cggagatcct aaaaatgcgg
     3541 atgacacatc gatttacatg ttctatcaaa aagtcggcga aacttctatt gacagctgga
     3601 aaaacgctgg ccgcgtcttt aaagacagcg acaaattcga tgcaaatgat tctatcctaa
     3661 aagaccaaac acaagaatgg tcaggttcag ccacatttac atctgacgga aaaatccgtt
     3721 tattctacac tgatttctcc ggtaaacatt acggcaaaca aacactgaca actgcacaag
     3781 ttaacgtatc agcatcagac agctctttga acatcaacgg tgtagaggat tataaatcaa
     3841 tctttgacgg tgacggaaaa acgtatcaaa atgtacagca gttcatcgat gaaggcaact
     3901 acagctcagg cgacaaccat acgctgagag atcctcacta cgtagaagat aaaggccaca
     3961 aatacttagt atttgaagca aacactggaa ctgaagatgg ctaccaaggc gaagaatctt
     4021 tatttaacaa agcatactat ggcaaaagca catcattctt ccgtcaagaa agtcaaaaac
     4081 ttctgcaaag cgataaaaaa cgcacggctg agttagcaaa cggcgctctc ggtatgattg
     4141 agctaaacga tgattacaca ctgaaaaaag tgatgaaacc gctgattgca tctaacacag
     4201 taacagatga aattgaacgc gcgaacgtct ttaaaatgaa cggcaaatgg tacctgttca
     4261 ctgactcccg cggatcaaaa atgacgattg acggcattac gtctaacgat atttacatgc
     4321 ttggttatgt ttctaattct ttaactggcc catacaagcc gctgaacaaa actggccttg
     4381 tgttaaaaat ggatcttgat cctaacgatg taacctttac ttactcacac ttcgctgtac
     4441 ctcaagcgaa aggaaacaat gtcgtgatta caagctatat gacaaacaga ggattctacg
     4501 cagacaaaca atcaacgttt gcgccaagct tcctgctgaa catcaaaggc aagaaaacat
     4561 ctgttgtcaa agacagcatc cttgaacaag gacaattaac agttaacaaa taaaaacgca
     4621 aaagaaaatg ccgatggccg cggcgttgtg acaatttacc gaacaactcc gcggccggga
     4681 agccgatctc ggcttgaacg aattgCAGAt ctagggcggc ggatttgtcc tactcaggag
     4741 agcgttcacc gacaaacaac agataaaacg aaaggcccag tctttcgact gagcctttcg
     4801 ttttatttga tgcctgccga attcggatcc ggagcacgat cttgcggatg ccaagcttca
     4861 ccgctgcctc gatgacgtta taggtgccca tcgtattgat gcggaacgtc tcgttgtccg
     4921 gcttgatcag gatgcgcgga accgcggcaa aatggatcac cgcatcgtaa ggcaacacac
     4981 ccgtcccgct ttcaagctcc gaaaaatcgc gatgcatgga aagagcgtta aagacctgcc
     5041 cgctatcggt gatatcgacg atgagattgg taacaccctg cttgtcgagc gggacaagat
     5101 cgatgttgtg gacctcgtaa cccctgtcga tgagccacgg gatcacatgc ctgccagcct
     5161 tgccggaacc gccggtaaat agaatgcgct ttttcatgat actcctcatc atctccccag
     5221 caatgaagta gcagcccggg ccgaagagcg aaagccaccc ctcttccaag ccccaataca
     5281 aaacaaaaaa ccccgctgtt tccagcgggg tttccttcaa gccttttcag gcctgaaAAT
     5341 TACTCGACGA TCGAAGCAAC GATGCCGGCG CCGACGGTAC GGCCGCCTTC GCGGATAGCG
     5401 AAGCGCAGCT TTTCTTCCAT CGCGATCGGA ACGATCAGCT CGACTTCAAC AGTGACGTTG
     5461 TCGCCAGGCA TAACCATTTC CGTGCCTTCA GGAAGCGAAA CGATACCGGT AACGTCAGTC
     5521 GTACGGAAGT AGAACTGCGG ACGGTAGTTC GTGAAGAACG GCGTATGACG ACCGCCTTCT
     5581 TCCTTCGTCA GGATGTAGGC TTCTGCCATG AACTTCTTGT GCGGCTTGAC CGAACCCGGC
     5641 TTGCACAGGA TCTGACCACG CTCGACGCCG TCACGGGTAA CGCCGCGAAC GAGTGCACCG
     5701 ATGTTGTCGC CGGCCTGGCC CTGGTCGAGC AGCTTGCGGA ACATTTCAAC GCCGGTAACA
     5761 GTCGTCTTCG AGGTCGGACG GATGCCGACG ATTTCGACTT CTTCACCAAC CTTGACGATA
     5821 CCGCGCTCAA CGCGACCCGT CACAACCGTA CCACGACCCG AGATCGAGAA CACGTCTTCG
     5881 ATCGGCATCA GGAACGGCTG GTCGATCGGA CGCTCGGGCG TCGGGATGTA GGCGTCGACA
     5941 GCAGCCATCA GCTCGCGGAT CGCGTCTTCA CCGATCTTCT TGTCAGAATC TTCAAGAGCA
     6001 GCAAGTGCCG AACCCTTGAT GATCGGGATA TCGTCGCCCG GGAAGTCGTA GGACGACAGA
     6061 AGTTCGCGAA CTTCAAGCTC GACGAGCTCG AGAAGCTCGG CGTCGTCAAC CTGGTCGACC
     6121 TTGTTGAGGA ACACGACGAT GGCCGGAACG CCAACCTGAC GGGCAAGCAG GATGTGCTCG
     6181 CGGGTCTGTG GCATCGGGCC GTCGGCAGCC GAGCAAACCA GGATCGCGCC GTCCATCTGG
     6241 GCAGCACCGG TGATCATGTT CTTCACATAG TCGGCGTGGC CGGGGCAGTC AACGTGGGCG
     6301 TAGTGACGCG CAGGCGTCTC ATACTCAACG TGCGCCGTCG AAATCGTGAT ACCACGAGCC
     6361 TTTTCTTCAG GAGCGGCGTC GATTTGGTCA TACGCCTTGA ACTCGCCGAA ATACTTCGTG
     6421 ATCGCAGCAG TCAACGACGT CTTGCCGTGG TCGACGTGAC CGATCGTGCC gacgttgcaa
     6481 tgcgggagGG AACGATCAAA TTTTTCCTTT GCCATGTgaa ttgcttcctg tgaacgtgaa
     6541 gtcgatttcc cggcgaaccg gtttggtgtc gccgtttaag gctttgtaag cgaatgcgca
     6601 agacttaatt gtgaaagcgt cctgacgcgc gctccaagcg tgccccgagc cgcgtatatg
     6661 gtatatgttt tgcgacattt aaacccctgg agagggtgtg ccggggtagg cccgacgacc
     6721 cctctcccca agcattgccg atcactaatt gagatatttg catcacaact tctttaccgg
     6781 caccagcatg atggcttaag gacattccat cgccggggga gacggtcgat tggaacgaaa
     6841 cgcagggcac gccggccgag ctccgtatcg atccttcgct tttcctcgtg tccttattat
     6901 ccgtttcagg accattccat ggaaatcttc acagccgccg gcttcacggc attcttacag
     6961 gtcattgcga tcgatctcgt tctggccggc gacaacgcca tcgtcatcgg ccttgccgcc
     7021 gcgggccttc ccgcccatct tcgccgcaag gccattctgg tcggcatcat tgccgccacg
     7081 gttctgcgca tcggttttgc cgcagtcacc gtgcagctcc tcgccatcgg cggcctgcaa
     7141 ttgttcggcg gcctgatgct cgcctgggtg tgctggaaga tgtggacgga actgCGCT
//
