LOCUS       pAAV-hSyn-mem-se        5729 bp DNA     circular SYN 01-APR-2025
DEFINITION  AAV vector encoding membrane-tethered seTurboID under the control of
            human synapsin promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5729)
  AUTHORS   Shilin Zhong
  TITLE     Direct Submission
  JOURNAL   Exported Apr 1, 2025 from SnapGene 7.1.2
            https://www.snapgene.com
COMMENT     Sequence Label: pAAV-hSyn-EGFP
FEATURES             Location/Qualifiers
     source          1..5729
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     promoter        150..572
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             592..1833
                     /label=mem-V5-seTurboID
                     /note="color: #ff99cc; direction: RIGHT"
     CDS             652..693
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /note="color: #cc99b2"
                     /translation="GKPIPNPLLGLDST"
     misc_feature    1861..2449
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
                     /note="color: #ffffff"
     CDS             complement(2332..2343)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #cc99b2
                     Cleavage site after base 2331"
                     /translation="IEGR"
     polyA_signal    2481..2957
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     repeat_region   2997..3137
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     rep_origin      3212..3667
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        3949..4053
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             join(4054..4122,4123..4914)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 4054 .. 4122 / #ccffcc / signal sequence
                        2: 4123 .. 4914 / #ccffcc
                     Cleavage site after base 4122"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5085..5673
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
ORIGIN
        1 catgtcctgc aggcagctgc gcgctcgctc gctcactgag gccgcccggg cgtcgggcga
       61 cctttggtcg cccggcctca gtgagcgagc gagcgcgcag agagggagtg gccaactcca
      121 tcactagggg ttcctgcggc cgcacgcgta tgaggcgggg tgggggtgcc tacctgacga
      181 ccgaccccga cccactggac aagcacccaa cccccattcc ccaaattgcg catcccctat
      241 cagagagggg gaggggaaac aggatgcggc gaggcgcgtg cgcactgcca gcttcagcac
      301 cgcggacagt gccttcgccc ccgcctggcg gcgcgcgcca ccgccgcctc agcactgaag
      361 gcgcgctgac gtcactcgcc ggtcccccgc aaactcccct tcccggccac cttggtcgcg
      421 tccgcgccgc cgccggccca gccggaccgc accacgcgag gcgcgagata ggggggcacg
      481 ggcgcgacca tctgcgctgc ggcgccggcg actcagcgct gcctcagtct gcggtgggca
      541 gcggaggagt cgtgtcgtgc ctgagagcgc agtcgagaaa ccggtgccac catgctgtgc
      601 tgtatgagaa gaaccaaaca ggttgaaaag aatgatgagg accaaaagat cggcaagccc
      661 atccccaacc ccctgctggg cctggacagc accgctagca aagacaatac tgtgcctctg
      721 aagctgatcg ctctcctggc taatggcgag ttccatagtg gcgaacagct gggagaaacc
      781 ctgggcatgt ccagggccgc tatcaacaag cacattcaga ctctgcgcga ctggggcgtg
      841 gacgtgttca ccgtgcccgg aaagggctac tctctgcccg agcctatccc gctgctgaac
      901 gctaaacaga ttctgggaca gctggacggc gggagcgtgg cagtcctgcc tgtggtcgac
      961 tccaccaatc agtacctgct ggatcgaatc ggcgagctga agagtgggga tgcttgcatt
     1021 gcagaatatc agcaggcagg gagaggaagc agagggagga aatggttctc tccttttgga
     1081 gctaacctgt acctgagtat gttttggcgc ctgaagcggg gaccagcagc aatcggcctg
     1141 ggcccggtca tcggaattgt catggcagaa gcgctgcgaa agctgggagc agacaaggtg
     1201 cgagtcaaat ggcccaatga cctgtatctg caggatagaa agctggcagg catcctggtg
     1261 gagctggccg gaataacagg cgatgctgca cagatcgtca ttggcgccgg gattaacgtg
     1321 gctatgaggc gcgtggagga aagcgtggtc aatcagggct ggatcacact gcaggaagca
     1381 gggattaacc tggacaggaa tactctggcc gctacgctga tccgagagct gcgggcagcc
     1441 ctggaactgt tcgagcagga aggcctggct ccatatctgc cacggtggga gaagctggat
     1501 aacttcatca atagacccgt gaagctgatc attggggaca aagagatttt cgggattagc
     1561 cgggggattg ataaacaggg agccctgctg ctggaacagg acggagttat caaaccctgg
     1621 atgggcggag aaatcagtct gcggtctgcc gaaaagggcg gtggcggatc ttacaagctt
     1681 atcctgaacg gtaaaaccct gaaaggtgaa accaccaccg aagctgttga cgctgctacc
     1741 gcggaaaaag ttttcaaaca gtacgctaac gacaacggtg ttgacggtga atggacctac
     1801 gacgacgcta ccaaaacctt cacggtaacc gaataagaat tcgatatcaa gcttatcgat
     1861 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     1921 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     1981 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     2041 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     2101 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     2161 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     2221 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     2281 gcctatgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     2341 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     2401 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccga
     2461 gcgctgctcg agagatctac gggtggcatc cctgtgaccc ctccccagtg cctctcctgg
     2521 ccctggaagt tgccactcca gtgcccacca gccttgtcct aataaaatta agttgcatca
     2581 ttttgtctga ctaggtgtcc ttctataata ttatggggtg gaggggggtg gtatggagca
     2641 aggggcaagt tgggaagaca acctgtaggg cctgcggggt ctattgggaa ccaagctgga
     2701 gtgcagtggc acaatcttgg ctcactgcaa tctccgcctc ctgggttcaa gcgattctcc
     2761 tgcctcagcc tcccgagttg ttgggattcc aggcatgcat gaccaggctc agctaatttt
     2821 tgtttttttg gtagagacgg ggtttcacca tattggccag gctggtctcc aactcctaat
     2881 ctcaggtgat ctacccacct tggcctccca aattgctggg attacaggcg tgaaccactg
     2941 ctcccttccc tgtccttctg attttgtagg taaccacgtg cggaccgagc ggccgcagga
     3001 acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg
     3061 gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc
     3121 gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     3181 ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg
     3241 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgcctt agcgcccgct
     3301 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     3361 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     3421 cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     3481 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     3541 aactctatct cgggctattc ttttgattta taagggattt tgccgatttc ggtctattgg
     3601 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt
     3661 acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
     3721 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     3781 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     3841 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     3901 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     3961 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     4021 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     4081 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     4141 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     4201 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     4261 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     4321 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     4381 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     4441 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     4501 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     4561 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     4621 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     4681 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     4741 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     4801 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     4861 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     4921 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     4981 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     5041 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     5101 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     5161 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     5221 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     5281 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     5341 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     5401 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     5461 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     5521 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     5581 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     5641 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     5701 ttcctggcct tttgctggcc ttttgctca
//
