LOCUS       mini-Sdd9-QBEmax        8801 bp DNA     circular SYN 02-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    mini-Sdd9-QBEmax
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 2, 2025 from SnapGene 6.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8801
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(171..1031)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1032..1136)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        1406..1785
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1786..1989
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             2031..7490
                     /codon_start=1
                     /label=QBEmax
                     /translation="MKRTADGSEFESPKKKRKVKATAKYFFYSNIMNFFKTEITLANGE
                     IRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRN
                     SDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSF
                     EKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYV
                     NFLYLASHYEKSGGSSGGSSGSETPGTSESATPESSGGSSGGSCPIWVKNALQELVGRK
                     ETSGKVFDVDGKPIGPDIQSGYKDRELRRGVYETLRKSPFFQKHFPSTATWYVSLHVEA
                     QYAVWMLRNRIKHATVVINNTYVCSDMNRLHDNCMTAVPHILPEGYTMTVWKADRTEVT
                     LRGKAPKESGGSSGGSSGSETPGTSESATPESSGGSSGGSLKGSPEDNEQKQLFVEQHK
                     HYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPA
                     AFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGDGGSGGSGGSGGSG
                     GSGGSGGDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLF
                     DSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKK
                     HERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIE
                     GDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLP
                     GEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADL
                     FLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYK
                     EIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDN
                     GSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMT
                     RKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELT
                     KVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVE
                     DRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLF
                     DDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSL
                     TFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVI
                     EMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGR
                     DMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKM
                     KNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSR
                     MNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTAL
                     IKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGSGGSGGSGGSTNLSDIIEKETGKQLV
                     IQESILMLPEEVEEVIGNKPESDILVHTAYDESTDENVMLLTSDAPEYKPWALVIQDSN
                     GENKIKMLSGGSGGSGGSTNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDIL
                     VHTAYDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKMLSGGSKRTADGSEFEPKK
                     KRKV"
     CDS             2730..2825
                     /codon_start=1
                     /label=32aa linker
                     /translation="SGGSSGGSSGSETPGTSESATPESSGGSSGGS"
     CDS             2826..3251
                     /codon_start=1
                     /label=mini-Sdd9
                     /translation="CPIWVKNALQELVGRKETSGKVFDVDGKPIGPDIQSGYKDRELRR
                     GVYETLRKSPFFQKHFPSTATWYVSLHVEAQYAVWMLRNRIKHATVVINNTYVCSDMNR
                     LHDNCMTAVPHILPEGYTMTVWKADRTEVTLRGKAPKE"
     CDS             3252..3347
                     /codon_start=1
                     /label=32aa linker
                     /translation="SGGSSGGSSGSETPGTSESATPESSGGSSGGS"
     CDS             3720..3779
                     /standard_name="Linker"
                     /label=20aa linker
                     /label=Linker
                     /note="color: #993366; direction: RIGHT"
     polyA_signal    7516..7740
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7811..7827)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7835..7851
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7859..7889)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7904..7925
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(8213..8801)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacactcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atcgatctcc cgatccccta
     1201 gggtcgactc tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct
     1261 gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa
     1321 ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc
     1381 gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca
     1441 attacggggt cattagttca tagcccatat attgagttcc gcgttacata acttacggta
     1501 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     1561 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     1621 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     1681 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     1741 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
     1801 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
     1861 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
     1921 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
     1981 agcagagctg gtttagtgaa ccgtcagatc cgctagagat ccgcggccgc atgaaaagaa
     2041 ccgctgatgg cagcgagttc gagagcccta agaagaagcg gaaggtgaag gctaccgcca
     2101 agtacttctt ctacagcaac atcatgaact ttttcaagac cgagattacc ctggccaacg
     2161 gcgagatccg gaagcggcct ctgatcgaga caaacggcga aaccggggag atcgtgtggg
     2221 ataagggccg ggattttgcc accgtgcgga aagtgctgag catgccccaa gtgaatatcg
     2281 tgaaaaagac cgaggtgcag acaggcggct tcagcaaaga gtctatcctg cccaagagga
     2341 acagcgataa gctgatcgcc agaaagaagg actgggaccc taagaagtac ggcggcttcg
     2401 acagccccac cgtggcctat tctgtgctgg tggtggccaa agtggaaaag ggcaagtcca
     2461 agaaactgaa gagtgtgaaa gagctgctgg ggatcaccat catggaaaga agcagcttcg
     2521 agaagaatcc catcgacttt ctggaagcca agggctacaa agaagtgaaa aaggacctga
     2581 tcatcaagct gcctaagtac tccctgttcg agctggaaaa cggccggaag agaatgctgg
     2641 cctctgccgg cgaactgcag aagggaaacg aactggccct gccctccaaa tatgtgaact
     2701 tcctgtacct ggccagccac tatgagaagt ctggaggatc tagcggagga tcctctggaa
     2761 gcgagacacc aggcacaagc gagtccgcca caccagagag ctccggcggc tcctccggag
     2821 gatcctgtcc catctgggtc aagaacgccc tgcaagagct ggtgggccgg aaggagacaa
     2881 gcggcaaggt gtttgatgtg gatggaaagc ccattggccc tgacatccaa agcggctaca
     2941 aagaccggga actgagaaga ggtgtgtatg aaaccctcag aaagtccccc ttcttccaga
     3001 agcacttccc tagcacagcc acctggtacg tgagcctcca cgtggaagcc cagtacgctg
     3061 tgtggatgct gaggaacaga atcaaacatg ctaccgtggt gatcaacaac acatacgtct
     3121 gctctgacat gaaccggctg catgacaact gcatgaccgc tgtcccacac attctgccag
     3181 agggctacac catgacagtc tggaaggctg acaggacaga ggtaacactt cggggcaaag
     3241 cccccaaaga atccggagga tctagcggag gctcctctgg ctctgagaca cctggcacaa
     3301 gcgagagcgc aacacctgaa agcagcgggg gcagcagcgg ggggtcactg aagggctccc
     3361 ccgaggataa tgagcagaaa cagctgtttg tggaacagca caagcactac ctggacgaga
     3421 tcatcgagca gatcagcgag ttctccaaga gagtgatcct ggccgacgct aatctggaca
     3481 aagtgctgtc cgcctacaac aagcaccggg ataagcccat cagagagcag gccgagaata
     3541 tcatccacct gtttaccctg accaatctgg gagcccctgc cgccttcaag tactttgaca
     3601 ccaccatcga ccggaagagg tacaccagca ccaaagaggt gctggacgcc accctgatcc
     3661 accagagcat caccggcctg tacgagacac ggatcgacct gtctcagctg ggaggtgacg
     3721 gcggatcagg aggctctggc ggttcaggtg gatcaggcgg tagcggaggt tcaggtggtg
     3781 acaagaagta cagcatcggc ctggccatcg gcaccaactc tgtgggctgg gccgtgatca
     3841 ccgacgagta caaggtgccc agcaagaaat tcaaggtgct gggcaacacc gaccggcaca
     3901 gcatcaagaa gaacctgatc ggagccctgc tgttcgacag cggcgaaaca gccgaggcca
     3961 cccggctgaa gagaaccgcc agaagaagat acaccagacg gaagaaccgg atctgctatc
     4021 tgcaagagat cttcagcaac gagatggcca aggtggacga cagcttcttc cacagactgg
     4081 aagagtcctt cctggtggaa gaggataaga agcacgagcg gcaccccatc ttcggcaaca
     4141 tcgtggacga ggtggcctac cacgagaagt accccaccat ctaccacctg agaaagaaac
     4201 tggtggacag caccgacaag gccgacctgc ggctgatcta tctggccctg gcccacatga
     4261 tcaagttccg gggccacttc ctgatcgagg gcgacctgaa ccccgacaac agcgacgtgg
     4321 acaagctgtt catccagctg gtgcagacct acaaccagct gttcgaggaa aaccccatca
     4381 acgccagcgg cgtggacgcc aaggccatcc tgtctgccag actgagcaag agcagacggc
     4441 tggaaaatct gatcgcccag ctgcccggcg agaagaagaa tggcctgttc ggaaacctga
     4501 ttgccctgag cctgggcctg acccccaact tcaagagcaa cttcgacctg gccgaggatg
     4561 ccaaactgca gctgagcaag gacacctacg acgacgacct ggacaacctg ctggcccaga
     4621 tcggcgacca gtacgccgac ctgtttctgg ccgccaagaa cctgtccgac gccatcctgc
     4681 tgagcgacat cctgagagtg aacaccgaga tcaccaaggc ccccctgagc gcctctatga
     4741 tcaagagata cgacgagcac caccaggacc tgaccctgct gaaagctctc gtgcggcagc
     4801 agctgcctga gaagtacaaa gagattttct tcgaccagag caagaacggc tacgccggct
     4861 acattgacgg cggagccagc caggaagagt tctacaagtt catcaagccc atcctggaaa
     4921 agatggacgg caccgaggaa ctgctcgtga agctgaacag agaggacctg ctgcggaagc
     4981 agcggacctt cgacaacggc agcatccccc accagatcca cctgggagag ctgcacgcca
     5041 ttctgcggcg gcaggaagat ttttacccat tcctgaagga caaccgggaa aagatcgaga
     5101 agatcctgac cttccgcatc ccctactacg tgggccctct ggccagggga aacagcagat
     5161 tcgcctggat gaccagaaag agcgaggaaa ccatcacccc ctggaacttc gaggaagtgg
     5221 tggacaaggg cgcttccgcc cagagcttca tcgagcggat gaccaacttc gataagaacc
     5281 tgcccaacga gaaggtgctg cccaagcaca gcctgctgta cgagtacttc accgtgtata
     5341 acgagctgac caaagtgaaa tacgtgaccg agggaatgag aaagcccgcc ttcctgagcg
     5401 gcgagcagaa aaaggccatc gtggacctgc tgttcaagac caaccggaaa gtgaccgtga
     5461 agcagctgaa agaggactac ttcaagaaaa tcgagtgctt cgactccgtg gaaatctccg
     5521 gcgtggaaga tcggttcaac gcctccctgg gcacatacca cgatctgctg aaaattatca
     5581 aggacaagga cttcctggac aatgaggaaa acgaggacat tctggaagat atcgtgctga
     5641 ccctgacact gtttgaggac agagagatga tcgaggaacg gctgaaaacc tatgcccacc
     5701 tgttcgacga caaagtgatg aagcagctga agcggcggag atacaccggc tggggcaggc
     5761 tgagccggaa gctgatcaac ggcatccggg acaagcagtc cggcaagaca atcctggatt
     5821 tcctgaagtc cgacggcttc gccaacagaa acttcatgca gctgatccac gacgacagcc
     5881 tgacctttaa agaggacatc cagaaagccc aggtgtccgg ccagggcgat agcctgcacg
     5941 agcacattgc caatctggcc ggcagccccg ccattaagaa gggcatcctg cagacagtga
     6001 aggtggtgga cgagctcgtg aaagtgatgg gccggcacaa gcccgagaac atcgtgatcg
     6061 aaatggccag agagaaccag accacccaga agggacagaa gaacagccgc gagagaatga
     6121 agcggatcga agagggcatc aaagagctgg gcagccagat cctgaaagaa caccccgtgg
     6181 aaaacaccca gctgcagaac gagaagctgt acctgtacta cctgcagaat gggcgggata
     6241 tgtacgtgga ccaggaactg gacatcaacc ggctgtccga ctacgatgtg gaccatatcg
     6301 tgcctcagag ctttctgaag gacgactcca tcgacaacaa ggtgctgacc agaagcgaca
     6361 agaaccgggg caagagcgac aacgtgccct ccgaagaggt cgtgaagaag atgaagaact
     6421 actggcggca gctgctgaac gccaagctga ttacccagag aaagttcgac aatctgacca
     6481 aggccgagag aggcggcctg agcgaactgg ataaggccgg cttcatcaag agacagctgg
     6541 tggaaacccg gcagatcaca aagcacgtgg cacagatcct ggactcccgg atgaacacta
     6601 agtacgacga gaatgacaag ctgatccggg aagtgaaagt gatcaccctg aagtccaagc
     6661 tggtgtccga tttccggaag gatttccagt tttacaaagt gcgcgagatc aacaactacc
     6721 accacgccca cgacgcctac ctgaacgccg tcgtgggaac cgccctgatc aaaaagtacc
     6781 ctaagctgga aagcgagttc gtgtacggcg actacaaggt gtacgacgtg cggaagatga
     6841 tcgccaagag cgagcaggaa atcggcagcg gcgggagcgg cgggagcggg gggagcacta
     6901 atctgagcga catcattgag aaggagactg ggaaacagct ggtcattcag gagtccatcc
     6961 tgatgctgcc tgaggaggtg gaggaagtga tcggcaacaa gccagagtct gacatcctgg
     7021 tgcacaccgc ctacgacgag tccacagatg agaatgtgat gctgctgacc tctgacgccc
     7081 ccgagtataa gccttgggcc ctggtcatcc aggattctaa cggcgagaat aagatcaaga
     7141 tgctgagcgg aggatccgga ggatctggag gcagcaccaa cctgtctgac atcatcgaga
     7201 aggagacagg caagcagctg gtcatccagg agagcatcct gatgctgccc gaagaagtcg
     7261 aagaagtgat cggaaacaag cctgagagcg atatcctggt ccataccgcc tacgacgaga
     7321 gtaccgacga aaatgtgatg ctgctgacat ccgacgcccc agagtataag ccctgggctc
     7381 tggtcatcca ggattccaac ggagagaaca aaatcaaaat gctgagcggc ggcagcaaaa
     7441 gaaccgccga cggctcagag ttcgagccca aaaagaagcg gaaagtgtaa gtttaaaccc
     7501 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     7561 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     7621 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     7681 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     7741 cttctgaggc ggaaagaacc agctggggct cgataccgtc gacctctagc tagagcttgg
     7801 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     7861 acatacgagc cggaagcata aagtgtaaag cctagggtgc ctaatgagtg agctaactca
     7921 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     7981 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     8041 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     8101 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     8161 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     8221 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     8281 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     8341 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     8401 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     8461 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     8521 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     8581 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     8641 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     8701 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     8761 tttgcaagca gcagattacg cgcagaaaaa aaggatctca a
//
