LOCUS       p6_msp6                 4051 bp    DNA     circular SYN 01-APR-2025
DEFINITION  p6_msp6.
ACCESSION   p6_msp6
VERSION     p6_msp6
KEYWORDS    .
SOURCE      
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     CDS             2034..2825
                     /note="pLannotate"
                     /label="neo"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     terminator      2859..2953
                     /note="pLannotate"
                     /label="lambda t0 terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     misc_RNA        complement(1594..1669)
                     /note="pLannotate"
                     /label="gRNA scaffold"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_RNA"
     promoter        complement(1690..1763)
                     /note="pLannotate"
                     /label="PLtetO-1 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     terminator      complement(3063..3134)
                     /note="pLannotate"
                     /label="rrnB T1 terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     CDS             complement(3146..3859)
                     /note="pLannotate"
                     /label="superfolder GFP"
                     /database="snapgene"
                     /identity="99.7"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      1..589
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     terminator      complement(1239..1285)
                     /note="pLannotate"
                     /label="rrnB T1 terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     terminator      1781..1824
                     /note="pLannotate"
                     /label="bacterial terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     promoter        complement(3921..3951)
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     oriT            complement(784..1074)
                     /note="pLannotate"
                     /label="oriT"
                     /database="snapgene"
                     /identity="99.7"
                     /match_length="100.0"
                     /fragment="False"
                     /other="oriT"
     terminator      complement(3020..3047)
                     /note="pLannotate"
                     /label="T7Te terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     protein_bind    complement(3966..3987)
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     protein_bind    complement(3897..3913)
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     ncRNA           complement(1287..1402)
                     /note="pLannotate"
                     /label="5S rRNA"
                     /database="Rfam"
                     /identity="100.0"
                     /match_length="97.5"
                     /fragment="False"
                     /other="ncRNA"
     rep_origin      1124..1191
                     /note="pLannotate"
                     /label="RSF ori (fragment)"
                     /database="snapgene"
                     /identity="95.6"
                     /match_length="9.1"
                     /fragment="True"
                     /other="rep_origin"
     CDS             1874..2002
                     /note="pLannotate"
                     /label="tnpA (fragment)"
                     /database="swissprot"
                     /identity="86.0"
                     /match_length="9.0"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(1511..1528)
                     /note="pLannotate"
                     /label="capTEV™ (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="6.3"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctgtggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgaggtt caccttagcc attagagatt
      721 accttttttg aacataaaat aaagttaatt tcgaaacact aaaattcata atgcaaacag
      781 ggacgcaccg ctagcagcgc ccctagcggt atcctataaa aaaacacacc gcgccgctag
      841 cagcacccct aatataaaat aatgtttttt ataaaaatag tcagtaccac ccctacaaaa
      901 cggtgtcggc gcgttgttgt agccgcgccg acaccgcttt tttaaatatc ataaagagag
      961 taagagaaac taatttttca taacactcta tttataaaga aaaatcagca aaaacttgtt
     1021 tttgcatggg gtgtggtgct tttggtggtg agaaccacca acctgttgag cctttttgtg
     1081 gagtgggtta aattatttac gaataaagtc accagaggtg gcctttgagt gagctgatac
     1141 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cctgcagtct
     1201 agactcgagt aaggatccag ttcaccgaca aacaacagat aaaacgaaag gcccagtctt
     1261 tcgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc atggggagac
     1321 cccacactac catcggcgct acggcgtttc acttctgagt tcggcatggg gtcaggtggg
     1381 accaccgcgc tactgccgcc aggcaaattc tgttttatca gaccgcttct gcgttctgat
     1441 ttaatctgta tcaggctgaa aatcttctct catccgccaa aacagccaag ctggagaccg
     1501 tttaaactca atgatgatga tgatgatggt cgacggcgct attcagatcc tcttctgaga
     1561 tgagtttttg ttcgggccca agcttcaaaa aaagcaccga ctcggtgcca ctttttcaag
     1621 ttgataacgg actagcctta ttttaacttg ctatttctag ctctaaaacc gtgaaacgcc
     1681 tattcttatg gtcagtgcgt cctgctgatg tgctcagtat ctctatcact gatagggatg
     1741 tcaatctcta tcactgatag ggaagatctt tagaattcca gaaatcatcc ttagcgaaag
     1801 ctaaggattt tttttatctg aaattctgcc tcgtgatacg cctattttta taggttaatg
     1861 tcatgataat aatggtttct tagacgtcag ggacgtcgga attgccagct ggggcgccct
     1921 ctggtaaggt tgggaagccc tgcaaagtaa actggatggc tttcttgccg ccaaggatct
     1981 gatggcgcag gggatcaaga tctgatcaag agacaggatg aggatcgttt cgcatgattg
     2041 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
     2101 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
     2161 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg
     2221 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
     2281 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
     2341 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
     2401 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
     2461 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
     2521 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg
     2581 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
     2641 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
     2701 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
     2761 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
     2821 tcttctgagc gggactctgg ggttcgagag ctcgcttgga ctcctgttga tagatccagt
     2881 aatgacctca gaactccatc tggatttgtt cagaacgctc ggttgccgcc gggcgttttt
     2941 tattggtgag aatccaagca ctagtaacaa cttatatcgt atggggctga cttcaggtgc
     3001 taccgtggag acgtataaac gcagaaaggc ccacccgaag gtgagccagt gtgactctag
     3061 tagagagcgt tcaccgacaa acaacagata aaacgaaagg cccagtcttt cgactgagcc
     3121 tttcgtttta tttgatgcct ggtcatttgt acagttcatc cataccatgc gtgatgcccg
     3181 ctgcggttac gaactccagc agaaccatat gatcgcgttt ctcgttcgga tctttagaca
     3241 gaacgctttg cgtgctcaga tagtgattgt ctggcagcag aacaggacca tcaccgattg
     3301 gagtgttttg ctggtagtga tcagccagct gcacgctgcc atcctccacg ttgtggcgaa
     3361 ttttaaaatt cgctttaatg ccattttttt gtttatcggc ggtgatgtaa acattgtggc
     3421 tgttaaaatt gtattccagc ttatggccca ggatattgcc gtcctcttta aagtcaatgc
     3481 ctttcagctc aatgcggttt accagggtat cgccttcaaa tttcacttcc gcacgcgttt
     3541 tgtacgtgcc gtcatcctta aaggaaatcg tgcgttcctg cacatagcct tccggcatgg
     3601 cggacttgaa gaagtcatgc tgcttcatat ggtccggata acgagcaaag cactgaacac
     3661 cataagtcag cgtcgttacc agagtcggcc aaggaaccgg cagtttacca gtagtacaga
     3721 tgaacttcag cgtcagttta ccattagttg cgtcaccttc accctcgcca cgcacggaaa
     3781 acttatgacc gttgacatca ccatccagtt ccaccagaat agggacgaca ccagtgaaca
     3841 gctcttcgcc tttacgcatc tagtatttct cctctttagc tgtttcctgt gtgaaattgt
     3901 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     3961 gcctaatgag tgagctaact cacattaatt gcgttgcgca gttttcgttc cactgagcgt
     4021 cagaccccgt agaaaagatc aaaggatctt c
//
