LOCUS       p12_pTarget_tetR        5018 bp    DNA     circular SYN 01-APR-2025
DEFINITION  p12_pTarget_tetR.
ACCESSION   p12_pTarget_tetR
VERSION     p12_pTarget_tetR
KEYWORDS    .
SOURCE      
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     CDS             complement(3156..3782)
                     /note="pLannotate"
                     /label="TetR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             2034..2825
                     /note="pLannotate"
                     /label="neo"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     terminator      2859..2953
                     /note="pLannotate"
                     /label="lambda t0 terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     terminator      complement(3064..3150)
                     /note="pLannotate"
                     /label="rrnB T1 terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     misc_RNA        complement(1594..1669)
                     /note="pLannotate"
                     /label="gRNA scaffold"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_RNA"
     promoter        complement(1690..1763)
                     /note="pLannotate"
                     /label="PLtetO-1 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     terminator      complement(4054..4125)
                     /note="pLannotate"
                     /label="rrnB T1 terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     CDS             complement(4137..4850)
                     /note="pLannotate"
                     /label="superfolder GFP"
                     /database="snapgene"
                     /identity="99.7"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      1..589
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     terminator      complement(1239..1285)
                     /note="pLannotate"
                     /label="rrnB T1 terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     terminator      1781..1824
                     /note="pLannotate"
                     /label="bacterial terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     oriT            complement(784..1074)
                     /note="pLannotate"
                     /label="oriT"
                     /database="snapgene"
                     /identity="99.7"
                     /match_length="100.0"
                     /fragment="False"
                     /other="oriT"
     terminator      complement(4011..4038)
                     /note="pLannotate"
                     /label="T7Te terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     ncRNA           complement(1287..1402)
                     /note="pLannotate"
                     /label="5S rRNA"
                     /database="Rfam"
                     /identity="100.0"
                     /match_length="97.5"
                     /fragment="False"
                     /other="ncRNA"
     rep_origin      1124..1191
                     /note="pLannotate"
                     /label="RSF ori (fragment)"
                     /database="snapgene"
                     /identity="95.6"
                     /match_length="9.1"
                     /fragment="True"
                     /other="rep_origin"
     rep_origin      2978..3043
                     /note="pLannotate"
                     /label="RSF ori (fragment)"
                     /database="snapgene"
                     /identity="95.5"
                     /match_length="8.8"
                     /fragment="True"
                     /other="rep_origin"
     CDS             1874..2002
                     /note="pLannotate"
                     /label="tnpA (fragment)"
                     /database="swissprot"
                     /identity="86.0"
                     /match_length="9.0"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(1511..1528)
                     /note="pLannotate"
                     /label="capTEV™ (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="6.3"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctgtggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgaggtt caccttagcc attagagatt
      721 accttttttg aacataaaat aaagttaatt tcgaaacact aaaattcata atgcaaacag
      781 ggacgcaccg ctagcagcgc ccctagcggt atcctataaa aaaacacacc gcgccgctag
      841 cagcacccct aatataaaat aatgtttttt ataaaaatag tcagtaccac ccctacaaaa
      901 cggtgtcggc gcgttgttgt agccgcgccg acaccgcttt tttaaatatc ataaagagag
      961 taagagaaac taatttttca taacactcta tttataaaga aaaatcagca aaaacttgtt
     1021 tttgcatggg gtgtggtgct tttggtggtg agaaccacca acctgttgag cctttttgtg
     1081 gagtgggtta aattatttac gaataaagtc accagaggtg gcctttgagt gagctgatac
     1141 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cctgcagtct
     1201 agactcgagt aaggatccag ttcaccgaca aacaacagat aaaacgaaag gcccagtctt
     1261 tcgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc atggggagac
     1321 cccacactac catcggcgct acggcgtttc acttctgagt tcggcatggg gtcaggtggg
     1381 accaccgcgc tactgccgcc aggcaaattc tgttttatca gaccgcttct gcgttctgat
     1441 ttaatctgta tcaggctgaa aatcttctct catccgccaa aacagccaag ctggagaccg
     1501 tttaaactca atgatgatga tgatgatggt cgacggcgct attcagatcc tcttctgaga
     1561 tgagtttttg ttcgggccca agcttcaaaa aaagcaccga ctcggtgcca ctttttcaag
     1621 ttgataacgg actagcctta ttttaacttg ctatttctag ctctaaaacc gtgatacgcc
     1681 tatttttatg gtcagtgcgt cctgctgatg tgctcagtat ctctatcact gatagggatg
     1741 tcaatctcta tcactgatag ggaagatctt tagaattcca gaaatcatcc ttagcgaaag
     1801 ctaaggattt tttttatctg aaattctgcc tcgtgatacg cctattttta taggttaatg
     1861 tcatgataat aatggtttct tagacgtcag ggacgtcgga attgccagct ggggcgccct
     1921 ctggtaaggt tgggaagccc tgcaaagtaa actggatggc tttcttgccg ccaaggatct
     1981 gatggcgcag gggatcaaga tctgatcaag agacaggatg aggatcgttt cgcatgattg
     2041 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
     2101 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
     2161 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg
     2221 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
     2281 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
     2341 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
     2401 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
     2461 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
     2521 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg
     2581 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
     2641 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
     2701 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
     2761 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
     2821 tcttctgagc gggactctgg ggttcgagag ctcgcttgga ctcctgttga tagatccagt
     2881 aatgacctca gaactccatc tggatttgtt cagaacgctc ggttgccgcc gggcgttttt
     2941 tattggtgag aatccaagca ctagtaacaa cttatatcct gattctgtgg ataaccgtat
     3001 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgccctagg tctagggcgg
     3061 cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca
     3121 gtctttcgac tgagcctttc gttttatttg atgccttaag acccactttc acatttaagt
     3181 tgtttttcta atccgcatat gatcaattca aggccgaata agaaggctgg ctctgcacct
     3241 tggtgatcaa ataattcgat agcttgtcgt aataatggcg gcatactatc agtagtaggt
     3301 gtttcccttt cttctttagc gacttgatgc tcttgatctt ccaatacgca acctaaagta
     3361 aaatgcccca ctgcgctgag tgcatataat gcattctcta gtgaaaaacc ttgttggcat
     3421 aaaaaggcta attgattttc gagagtttca tactgttttt ctgtaggccg tgtacctaaa
     3481 tgtacttttg ctccatcgcg atgacttagt aaagcacatc taaaactttt agcgttatta
     3541 cgtaaaaaat cttgccagct ttccccttct aaagggcaaa agtgagtatg gtgcctatct
     3601 aacatctcaa tggctaaggc gtcgagcaaa gcccgcttat tttttacatg ccaatacaat
     3661 gtaggctgct ctacacctag cttctgggcg agtttacggg ttgttaaacc ttcgattccg
     3721 acctcattaa gcagctctaa tgcgctgtta atcactttac ttttatctaa acgagacatc
     3781 atctagtatt tctcctcttt acaggcattc tgtaaaaggc gaggcggcat aactatagtg
     3841 gttttccaca tatcaggcgt ggatttgatc tagcgcaaca aatattgact agctaaccag
     3901 ctcatttcag aatatttgcc agaaccgtta tgatgtcggc gcaaaaaaca ttatccagaa
     3961 cgggagtgcg tatggggctg acttcaggtg ctaccgtgga gacgtataaa cgcagaaagg
     4021 cccacccgaa ggtgagccag tgtgactcta gtagagagcg ttcaccgaca aacaacagat
     4081 aaaacgaaag gcccagtctt tcgactgagc ctttcgtttt atttgatgcc tggtcatttg
     4141 tacagttcat ccataccatg cgtgatgccc gctgcggtta cgaactccag cagaaccata
     4201 tgatcgcgtt tctcgttcgg atctttagac agaacgcttt gcgtgctcag atagtgattg
     4261 tctggcagca gaacaggacc atcaccgatt ggagtgtttt gctggtagtg atcagccagc
     4321 tgcacgctgc catcctccac gttgtggcga attttaaaat tcgctttaat gccatttttt
     4381 tgtttatcgg cggtgatgta aacattgtgg ctgttaaaat tgtattccag cttatggccc
     4441 aggatattgc cgtcctcttt aaagtcaatg cctttcagct caatgcggtt taccagggta
     4501 tcgccttcaa atttcacttc cgcacgcgtt ttgtacgtgc cgtcatcctt aaaggaaatc
     4561 gtgcgttcct gcacatagcc ttccggcatg gcggacttga agaagtcatg ctgcttcata
     4621 tggtccggat aacgagcaaa gcactgaaca ccataagtca gcgtcgttac cagagtcggc
     4681 caaggaaccg gcagtttacc agtagtacag atgaacttca gcgtcagttt accattagtt
     4741 gcgtcacctt caccctcgcc acgcacggaa aacttatgac cgttgacatc accatccagt
     4801 tccaccagaa tagggacgac accagtgaac agctcttcgc ctttacgcat ctagtatttc
     4861 tcctctttac aggcattctg taaaaggcga ggcggcataa ctatagtggt tttccacata
     4921 tcaggcgtgg atttgatcta gcgcaacaaa tattgacgcg ttgcgcagtt ttcgttccac
     4981 tgagcgtcag accccgtaga aaagatcaaa ggatcttc
//
