LOCUS       pGEMHE-RnCENPW        3454 bp DNA     circular SYN 28-JAN-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 3454)
  AUTHORS   Covid-19 WFH
  TITLE     Direct Submission
  JOURNAL   Exported Mar 27, 2025 from SnapGene 7.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3454
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     promoter        complement(63..167)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     551..570
                     /label=QCH_seq_primer_1
                     /note="color: black; sequence: CCTCTTCGCTATTACGCCAG"
     primer_bind     641..657
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     promoter        664..682
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     5'UTR           714..756
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     complement(758..778)
                     /label=Vector-W-R
                     /note="color: black; sequence: ctagcaccggggaattgatct; 
                     added: 2023-01-28"
     primer_bind     764..797
                     /label=InFu-RnW-F
                     /note="color: black; sequence: 
                     attccccggtgctagATGGTGCTCTCCACCACAG; added: 2023-01-28"
     misc_feature    779..1036
                     /label=Rn_CENP-W
                     /note="color: #ffcc00"
     primer_bind     complement(1010..1051)
                     /label=InFu-RnW-R
                     /note="color: black; sequence: 
                     ttagtggtaacctcaTCCTCTGCTCTTCTTCAGAATTACCTT; added: 
                     2023-01-28"
     primer_bind     1037..1068
                     /label=Vector-W-F
                     /note="color: black; sequence: 
                     tgaggttaccactaaaccagcctcaagaacac; added: 2023-01-28"
     misc_feature    1133..1147
                     /label=5Gly
                     /note="color: #ffff00"
     CDS             1148..1189
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /note="color: #cc99b2"
                     /translation="SGFANELGPRLMGK"
     primer_bind     1190..1221
                     /label=Vector-W-F
                     /note="color: black; sequence: 
                     tgaggttaccactaaaccagcctcaagaacac; added: 2023-01-28"
     primer_bind     complement(1190..1204)
                     /label=InFu-RnW-R
                     /note="color: black; sequence: 
                     ttagtggtaacctcaTCCTCTGCTCTTCTTCAGAATTACCTT; added: 
                     2023-01-28"
     promoter        complement(1434..1452)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     complement(1470..1486)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    1494..1510
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(join(1518..1524,1525..1542,1543..1548))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 1518 .. 1524 / #ffffff / -10
                        2: 1525 .. 1542 / #ffffff
                        3: 1543 .. 1548 / #ffffff / -35"
     protein_bind    1563..1584
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     rep_origin      complement(1872..2461)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
ORIGIN
        1 aaacaggaag gcaaatgccg aaaaaaggga ataagggcga cacggaaatg ttgaatactc
       61 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
      121 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
      181 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
      241 cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
      301 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
      361 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca
      421 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
      481 agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
      541 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      601 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa
      661 ttgtaatacg actcactata gggcgaatta attcgagctc ggtacccagc ttgcttgttc
      721 tttttgcaga agctcagaat aaacgctcaa ctttggcaga tcaattcccc ggtgctagat
      781 ggtgctctcc accacagtct gtaggcggat aaggcgcaag gcgccctgcg ccttcctcaa
      841 gcgcacctta aagcagaaga agccgcggct cagtctggag aagcgctgcg atctcctgat
      901 ccatttgaat tgcctactct ttgttcagaa attggcagaa gagtctagga caaatgcttg
      961 tgaaagtaaa tctggagtta tcaaaaagga tcatgtacag gcggcagcaa aggtaattct
     1021 gaagaagagc agaggatgag gttaccacta aaccagcctc aagaacaccc gaatgggctg
     1081 gttaccacta aaccagcctc aagaacagaa ccgtcaagat gagaagaccg tcggtggcgg
     1141 aggtggcagc ggattcgcta atgagctggg accgaggctt atgggaaagt gaggttacca
     1201 ctaaaccagc ctcaagaaca cccgaatgga gtctctaagc tacataatac caacttacac
     1261 tttacaaaat gttgtccccc aaaatgtagc cattcgtatc tgctcctaat aaaaagaaag
     1321 tttcttcaca ttctaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaacc cccccccccc
     1381 ccccctgcag gcgcgccgcc ggcgatttaa attaattaat ctagcttgag tattctatag
     1441 tgtcacctaa atagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     1501 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     1561 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     1621 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     1681 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     1741 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     1801 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     1861 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     1921 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     1981 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     2041 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     2101 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     2161 ttatccggta actatcgtct tgagtccaac ccggtaagac acgaccttat cgccactggc
     2221 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     2281 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     2341 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     2401 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     2461 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     2521 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     2581 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     2641 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     2701 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     2761 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     2821 ccggaagggc cgagcgcaga agtggtcctg caaactttat ccgcctccat ccagtctatt
     2881 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     2941 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     3001 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     3061 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     3121 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     3181 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     3241 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     3301 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     3361 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     3421 gggtgagccc tttcaccagc gtttctgggt gagc
//
