LOCUS       Exported               11336 bp ds-DNA     circular SYN 16-APR-2024
DEFINITION  synthetic circular DNA
ACCESSION   VB240311-1433waq
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11336)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Apr 7, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11336
                     /organism="synthetic DNA construct"
     promoter        1..229
                     /label=RSV  promoter
                     /note="/application_notes=Strong promoter; drives 
                     transcription
                     of viral RNA in packaging cells."
                     /note="/description=Rous sarcoma virus enhancer/promoter"
                     /note="/full_name=Rous sarcoma virus (RSV) 
                     enhancer/promoter"
                     /note="/official_designation=RSV"
                     /note="/uuid=3f2ddca7f0cb4015b6abcec8a25c834e"
     LTR             230..410
                     /label=5' LTR-Delta-U3
                     /note="/application_notes=Allows transcription of viral RNA
                     and
                     its packaging into virus."
                     /note="/description=Truncated HIV-1 5' long terminal 
                     repeat"
                     /note="/full_name=HIV-1 truncated 5' LTR"
                     /note="/official_designation=Delta5' LTR"
                     /note="/uuid=44156a3083604321be44066133e8ecb9"
     misc_feature    521..565
                     /label=Psi
                     /label=nonstandard type:  Miscellaneous
                     /note="/application_notes=Allows packaging of viral RNA 
                     into
                     virus."
                     /note="/description=HIV-1 packaging signal"
                     /note="/full_name=HIV-1 psi packaging signal"
                     /note="/official_designation=Psi"
                     /note="/uuid=284899413ff943a99f8210a11ad8c54e"
     misc_feature    1075..1308
                     /label=RRE
                     /label=nonstandard type:  Miscellaneous
                     /note="/application_notes=Rev protein binding site that 
                     allows
                     Rev-dependent nuclear export of viral RNA during viral 
                     packaging."
                     /note="/description=HIV-1 Rev response element"
                     /note="/full_name=HIV-1 Rev response element"
                     /note="/official_designation=RRE"
                     /note="/uuid=df07099e49674cf3aba3661b54e28bd7"
     misc_feature    1803..1920
                     /label=cPPT
                     /label=nonstandard type:  Miscellaneous
                     /note="/application_notes=Facilitates the nuclear import of
                     HIV-1
                     cDNA through a central DNA flap."
                     /note="/description=Central polypurine tract"
                     /note="/full_name=Central polypurine tract"
                     /note="/official_designation=cPPT"
                     /note="/uuid=f7cb29985f9f41a7a5924d3462266f42"
     promoter        1959..3137
                     /label=EF1A
                     /note="/application_notes=Strong promoter."
                     /note="/description=Human eukaryotic translation elongation
                     factor 1 alpha1 promoter"
                     /note="/full_name=Human eukaryotic translation elongation 
                     factor
                     1 alpha1 promoter"
                     /note="/official_designation=EF1A"
                     /note="/uuid=69c052aac71a4f49bab5b7ae1f46ace8"
     misc_feature    3162..3167
                     /label=Kozak
                     /label=nonstandard type:  Miscellaneous
                     /note="/application_notes=Facilitates translation 
                     initiation of
                     ATG start codon downstream of the Kozak sequence."
                     /note="/description=Kozak translation initiation sequence"
                     /note="/full_name=Kozak"
                     /note="/official_designation=Kozak"
                     /note="/uuid=5f18b25a1234443494a618363f09f052"
     CDS             3168..5582
                     /label={TRIP12-391-93-EGFP(co)*}
                     /note="/application_notes="
                     /note="/official_designation={TRIP12-391-93-EGFP(co)*}"
                     /note="/uuid=ce98b0aa6e3b46b48a781042919813e9"
     misc_feature    5621..6218
                     /label=WPRE
                     /label=nonstandard type:  Miscellaneous
                     /note="/application_notes=Enhances virus stability in 
                     packaging
                     cells, leading to higher titer of packaged virus; enhances 
                     higher expression of transgenes."
                     /note="/description=Woodchuck hepatitis virus 
                     posttranscriptional
                     regulatory element"
                     /note="/full_name=Woodchuck hepatitis virus 
                     posttranscriptional
                     regulatory element"
                     /note="/official_designation=WPRE"
                     /note="/uuid=0aedff8308474b369c622433538d7836"
     promoter        6237..6747
                     /label=mPGK promoter
                     /note="/application_notes=Medium-strength promoter."
                     /note="/description=Mouse phosphoglycerate kinase 1 
                     promoter"
                     /note="/full_name=Mouse phosphoglycerate kinase 1 promoter"
                     /note="/official_designation=mPGK promoter"
                     /note="/uuid=bce93795b5fd4173b8b9abc832edad80"
     CDS             6760..7785
                     /label=Hygro
                     /note="/application_notes=Allows cells to be resistant to
                     hygromycin B."
                     /note="/description=Hygromycin resistance gene"
                     /note="/full_name=Hygromycin resistance gene"
                     /note="/official_designation=Hygro"
                     /note="/uuid=9135dd0aa64645f3aa3c381325794022"
     LTR             7856..8090
                     /label=3' LTR-Delta-U3
                     /note="/application_notes=Allows packaging of viral RNA 
                     into
                     virus; self-inactivates the 5' LTR by a copying mechanism 
                     during viral genome integration; contains polyadenylation 
                     signal for transcription termination."
                     /note="/description=Truncated HIV-1 3' long terminal 
                     repeat"
                     /note="/full_name=HIV-1 truncated 3' LTR"
                     /note="/official_designation=DeltaU3/3' LTR"
                     /note="/uuid=c958755414c34372b909e20e81ebcef6"
     polyA_signal    8163..8297
                     /label=SV40 early pA
                     /note="/application_notes=Allows transcription termination 
                     and
                     polyadenylation of mRNA transcribed by Pol II RNA 
                     polymerase."
                     /note="/description=Simian virus 40 early polyadenylation 
                     signal"
                     /note="/full_name=SV40 early polyadenation signal"
                     /note="/official_designation=SV40 early pA"
                     /note="/uuid=2fcb13a62e704d8095b188d9625a6a94"
     CDS             9251..10111
                     /label=AmpiciIIin
                     /note="/application_notes=Allows E. coli to be resistant to
                     ampiciIIin."
                     /note="/description=AmpiciIIin resistance gene"
                     /note="/full_name=Ampicillin resistance gene"
                     /note="/official_designation=Ampicillin"
                     /note="/uuid=6a7994dc58024d80b36e4a85ad2e1d99"
     rep_origin      10282..10870
                     /direction=RIGHT
                     /label=pUC ori
                     /note="/application_notes=Facilitates plasmid replication 
                     in E.
                     coli; regulates high-copy plasmid number (500-700)."
                     /note="/description=pUC origin of replication"
                     /note="/full_name=pUC origin of replication"
                     /note="/official_designation=pUC ori"
                     /note="/uuid=64a8cb3def9f49deb6752795c2d893a8"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttggg ctccggtgcc cgtcagtggg
     1981 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg
     2041 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
     2101 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
     2161 ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg
     2221 gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg
     2281 tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt
     2341 aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg
     2401 tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt
     2461 aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg
     2521 gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg
     2581 cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac
     2641 gggggtagtc tcaagctggc cggcctgctc tggtgcctgg tctcgcgccg ccgtgtatcg
     2701 ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc
     2761 cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg
     2821 cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg
     2881 actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta
     2941 cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg
     3001 tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt
     3061 ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc
     3121 catttcaggt gtcgtgacaa gtttgtacaa aaaagcaggc tgccaccatg gaatctgaaa
     3181 gtgacgactc agagatgggg aggctgcagg ccctgctgga ggccagggga ctgcctcctc
     3241 acctgttcgg gcctctgggg cctagaatgt cccagctatt tcaccgcact atcggcagcg
     3301 gggcctccag caaggcccag cagctgctgc agggcctgca ggccagcgac gagtcccagc
     3361 agctgcaggc cgtgatcgag atgtgccagc tgctggtgat gggcaacgag gaaacactgg
     3421 gaggttttcc agtgaaatca gtggtgcctg ccctgatcac cctcctgcag atggagcaca
     3481 atttcgacat aatgaaccac gcatgcagag ccctgacata tatgatggag gccctgccca
     3541 gatcatccgc cgtggtggtg gacgccattc ccgtgtttct ggagaagctg caggtgatcc
     3601 agtgcatcga cgtggctgag caggccctga ccgccctgga gatgctgtcc agaagacact
     3661 ccaaagctat cctccaggcc ggcggcctgg ccgactgcct cctgtacctg gaattttttt
     3721 ccatcaacgc ccagcggaac gccctggcta ttgccgctaa ctgctgccag tccattacac
     3781 cagatgagtt ccatttcgtg gccgacagcc tcccactgct gactcagcgg ctgacccacc
     3841 aggacaagaa gtctgtggag agcacctgcc tgtgcttcgc ccggctggtg gacaacttcc
     3901 agcacgaaga gaacctgctg cagcaggtgg ccagcaagga cctgctgact aacgtgcagc
     3961 agctgctggt ggtgaccccc cccattctga gttctggcat gttcatcatg gtggtgagga
     4021 tgttcagcct tatgtgctct aactgcccca ctctggcagt gcagctgatg aagcagaata
     4081 tcgccgagac cctgcacttc ctgctgtgtg gcgcatccaa tggcagttgt caggagcaga
     4141 tcgacctggt gccacgctca ccccaggaac tgtacgagct gaccagcctg atttgtgaac
     4201 tgatgccctg cctgcctaag gagggcatct tcgccgttga taccatgctg aagaaaggca
     4261 atgcccagaa taccgacggg gccatctggc agtggcgtga tgatagaggc ctgtggcacc
     4321 cctataacag aatcgactcc agaatcatcg aacagatcaa cgaggacacc ggcacagcaa
     4381 gagctatcca gcggaagcct aatcccctgg ccaactccaa tacttccggt tatagcgaat
     4441 caaagaagga cgatgcccgc gcccagctga tgaaggagga ccccgagctg gccaaatcct
     4501 tcattaagac cctgttcgga gtgctgtacg aggtgtacag cagcagtgct ggccccgccg
     4561 tgaggcacaa atgtctgagg gctatcctgc gcattatcta cttcgccgat gccgagctgc
     4621 tgaaagacgt gctgaaaaac catgctgtgt cttcccacat cgcctccatg ctgagcagcc
     4681 aggacctgaa gatcgttgtg ggcgccctgc agatggccga aatcctgatg cagaagctgc
     4741 cagatatctt ctccgtgtat ttccgcaggg agggcgtgat gcatcaggtg aaacacctgg
     4801 ccgagagcga gtcctctggc gggggcggca gcgggggcgg cggctccggc ggcggcggtt
     4861 ctatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     4921 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     4981 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     5041 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
     5101 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     5161 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     5221 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     5281 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     5341 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     5401 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     5461 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     5521 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt
     5581 aaacccagct ttcttgtaca aagtggtgat aatcgaattc cgataatcaa cctctggatt
     5641 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     5701 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     5761 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     5821 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     5881 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     5941 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     6001 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct
     6061 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     6121 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     6181 cgagtcggat ctccctttgg gccgcctccc cgcatcggga attcccgcgg ttcgaattct
     6241 accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc
     6301 tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta
     6361 ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag
     6421 tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag
     6481 cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc
     6541 tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa
     6601 ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt
     6661 cctccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct
     6721 cttcctcatc tccgggcctt tcgacctcac gtggccacca tgaaaaagcc tgaactcacc
     6781 gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga cctgatgcag
     6841 ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg taggagggcg tggatatgtc
     6901 ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt
     6961 gcatcggccg cgctcccgat tccggaagtg cttgacattg gggaatttag cgagagcctg
     7021 acctattgca tctcccgccg tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa
     7081 ctgcccgctg ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc ggccgatctt
     7141 agccagacga gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg
     7201 cgtgatttca tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac
     7261 gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac
     7321 tgccccgaag tccggcacct cgtgcacgcg gatttcggct ccaacaatgt cctgacggac
     7381 aatggccgca taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac
     7441 gaggtcgcca acatcttctt ctggaggccg tggttggctt gtatggagca gcagacgcgc
     7501 tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc gtatatgctc
     7561 cgcattggtc ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct
     7621 tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca
     7681 caaatcgccc gcagaagcgc ggccgtctgg accgatggct gtgtagaagt actcgccgat
     7741 agtggaaacc gacgccccag cactcgtccg agggcaaagg aatagggtac ctttaagacc
     7801 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     7861 agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
     7921 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     7981 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     8041 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     8101 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     8161 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     8221 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     8281 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg
     8341 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     8401 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     8461 gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt
     8521 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     8581 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     8641 tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     8701 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     8761 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     8821 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     8881 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     8941 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     9001 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     9061 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     9121 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     9181 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     9241 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     9301 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     9361 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     9421 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     9481 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     9541 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     9601 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     9661 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     9721 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     9781 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     9841 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     9901 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     9961 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
    10021 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
    10081 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
    10141 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
    10201 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
    10261 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
    10321 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
    10381 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
    10441 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
    10501 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
    10561 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
    10621 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
    10681 aagcgccacg cttcccgaag agagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
    10741 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
    10801 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
    10861 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
    10921 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
    10981 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
    11041 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
    11101 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
    11161 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
    11221 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
    11281 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagctt
//
