LOCUS       Exported               10190 bp ds-DNA     circular SYN 16-APR-2024
DEFINITION  synthetic circular DNA
ACCESSION   VB240311-1442utx
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10190)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Apr 7, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10190
                     /organism="synthetic DNA construct"
     promoter        1..229
                     /label=RSV  promoter
                     /note="/application_notes=Strong promoter; drives 
                     transcription
                     of viral RNA in packaging cells."
                     /note="/description=Rous sarcoma virus enhancer/promoter"
                     /note="/full_name=Rous sarcoma virus (RSV) 
                     enhancer/promoter"
                     /note="/official_designation=RSV"
                     /note="/uuid=3f2ddca7f0cb4015b6abcec8a25c834e"
     LTR             230..410
                     /label=5' LTR-Delta-U3
                     /note="/application_notes=Allows transcription of viral RNA
                     and
                     its packaging into virus."
                     /note="/description=Truncated HIV-1 5' long terminal 
                     repeat"
                     /note="/full_name=HIV-1 truncated 5' LTR"
                     /note="/official_designation=Delta5' LTR"
                     /note="/uuid=44156a3083604321be44066133e8ecb9"
     misc_feature    521..565
                     /label=Psi
                     /label=nonstandard type:  Miscellaneous
                     /note="/application_notes=Allows packaging of viral RNA 
                     into
                     virus."
                     /note="/description=HIV-1 packaging signal"
                     /note="/full_name=HIV-1 psi packaging signal"
                     /note="/official_designation=Psi"
                     /note="/uuid=284899413ff943a99f8210a11ad8c54e"
     misc_feature    1075..1308
                     /label=RRE
                     /label=nonstandard type:  Miscellaneous
                     /note="/application_notes=Rev protein binding site that 
                     allows
                     Rev-dependent nuclear export of viral RNA during viral 
                     packaging."
                     /note="/description=HIV-1 Rev response element"
                     /note="/full_name=HIV-1 Rev response element"
                     /note="/official_designation=RRE"
                     /note="/uuid=df07099e49674cf3aba3661b54e28bd7"
     misc_feature    1803..1920
                     /label=cPPT
                     /label=nonstandard type:  Miscellaneous
                     /note="/application_notes=Facilitates the nuclear import of
                     HIV-1
                     cDNA through a central DNA flap."
                     /note="/description=Central polypurine tract"
                     /note="/full_name=Central polypurine tract"
                     /note="/official_designation=cPPT"
                     /note="/uuid=f7cb29985f9f41a7a5924d3462266f42"
     promoter        1959..3137
                     /label=EF1A
                     /note="/application_notes=Strong promoter."
                     /note="/description=Human eukaryotic translation elongation
                     factor 1 alpha1 promoter"
                     /note="/full_name=Human eukaryotic translation elongation 
                     factor
                     1 alpha1 promoter"
                     /note="/official_designation=EF1A"
                     /note="/uuid=69c052aac71a4f49bab5b7ae1f46ace8"
     misc_feature    3162..3167
                     /label=Kozak
                     /label=nonstandard type:  Miscellaneous
                     /note="/application_notes=Facilitates translation 
                     initiation of
                     ATG start codon downstream of the Kozak sequence."
                     /note="/description=Kozak translation initiation sequence"
                     /note="/full_name=Kozak"
                     /note="/official_designation=Kozak"
                     /note="/uuid=5f18b25a1234443494a618363f09f052"
     CDS             3168..4436
                     /label={DTX4-1-166-EGFP(co)*}
                     /note="/application_notes="
                     /note="/official_designation={DTX4-1-166-EGFP(co)*}"
                     /note="/uuid=db4c8a4e7f6c48d2bf25b41491701411"
     misc_feature    4475..5072
                     /label=WPRE
                     /label=nonstandard type:  Miscellaneous
                     /note="/application_notes=Enhances virus stability in 
                     packaging
                     cells, leading to higher titer of packaged virus; enhances 
                     higher expression of transgenes."
                     /note="/description=Woodchuck hepatitis virus 
                     posttranscriptional
                     regulatory element"
                     /note="/full_name=Woodchuck hepatitis virus 
                     posttranscriptional
                     regulatory element"
                     /note="/official_designation=WPRE"
                     /note="/uuid=0aedff8308474b369c622433538d7836"
     promoter        5091..5601
                     /label=mPGK promoter
                     /note="/application_notes=Medium-strength promoter."
                     /note="/description=Mouse phosphoglycerate kinase 1 
                     promoter"
                     /note="/full_name=Mouse phosphoglycerate kinase 1 promoter"
                     /note="/official_designation=mPGK promoter"
                     /note="/uuid=bce93795b5fd4173b8b9abc832edad80"
     CDS             5614..6639
                     /label=Hygro
                     /note="/application_notes=Allows cells to be resistant to
                     hygromycin B."
                     /note="/description=Hygromycin resistance gene"
                     /note="/full_name=Hygromycin resistance gene"
                     /note="/official_designation=Hygro"
                     /note="/uuid=9135dd0aa64645f3aa3c381325794022"
     LTR             6710..6944
                     /label=3' LTR-Delta-U3
                     /note="/application_notes=Allows packaging of viral RNA 
                     into
                     virus; self-inactivates the 5' LTR by a copying mechanism 
                     during viral genome integration; contains polyadenylation 
                     signal for transcription termination."
                     /note="/description=Truncated HIV-1 3' long terminal 
                     repeat"
                     /note="/full_name=HIV-1 truncated 3' LTR"
                     /note="/official_designation=DeltaU3/3' LTR"
                     /note="/uuid=c958755414c34372b909e20e81ebcef6"
     polyA_signal    7017..7151
                     /label=SV40 early pA
                     /note="/application_notes=Allows transcription termination 
                     and
                     polyadenylation of mRNA transcribed by Pol II RNA 
                     polymerase."
                     /note="/description=Simian virus 40 early polyadenylation 
                     signal"
                     /note="/full_name=SV40 early polyadenation signal"
                     /note="/official_designation=SV40 early pA"
                     /note="/uuid=2fcb13a62e704d8095b188d9625a6a94"
     CDS             8105..8965
                     /label=AmpiciIIin
                     /note="/application_notes=Allows E. coli to be resistant to
                     ampiciIIin."
                     /note="/description=AmpiciIIin resistance gene"
                     /note="/full_name=Ampicillin resistance gene"
                     /note="/official_designation=Ampicillin"
                     /note="/uuid=6a7994dc58024d80b36e4a85ad2e1d99"
     rep_origin      9136..9724
                     /direction=RIGHT
                     /label=pUC ori
                     /note="/application_notes=Facilitates plasmid replication 
                     in E.
                     coli; regulates high-copy plasmid number (500-700)."
                     /note="/description=pUC origin of replication"
                     /note="/full_name=pUC origin of replication"
                     /note="/official_designation=pUC ori"
                     /note="/uuid=64a8cb3def9f49deb6752795c2d893a8"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttggg ctccggtgcc cgtcagtggg
     1981 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg
     2041 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
     2101 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
     2161 ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg
     2221 gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg
     2281 tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt
     2341 aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg
     2401 tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt
     2461 aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg
     2521 gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg
     2581 cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac
     2641 gggggtagtc tcaagctggc cggcctgctc tggtgcctgg tctcgcgccg ccgtgtatcg
     2701 ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc
     2761 cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg
     2821 cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg
     2881 actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta
     2941 cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg
     3001 tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt
     3061 ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc
     3121 catttcaggt gtcgtgacaa gtttgtacaa aaaagcaggc tgccaccatg atgctgctgg
     3181 cctctgccgt ggtggtgtgg gagtggctga atgagcacgg acggtggaga ccatactcac
     3241 cagccgtgtc acatcatatc gaggccgtgg tgcgggccgg ccccagggct ggcgggtccg
     3301 ttgtcctggg tcaggtggac agcagactgg ccccatacat catcgacctg cagagcatga
     3361 accagtttcg acaggacaca ggcacactga gacctgtgcg gagaaactat tacgatccaa
     3421 gctcagcacc cggcaaaggc gtggtgtggg agtgggagaa tgacaacggg tcatggaccc
     3481 cttacgatat ggaggtgggc attacaatcc agcacgccta tgagaagcag cacccttgga
     3541 tcgacctgac atccatcggg ttcagctacg tgattgattt caacaccatg ggccagatca
     3601 ataggcagac ccagagacag cggagagtga ggagaagact ggatctgatc tatcccatgg
     3661 tgacaggcag tggcgggggc ggcagcggcg gaggcggctc cggaggcgga ggaagcatgg
     3721 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     3781 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     3841 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     3901 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
     3961 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     4021 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     4081 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     4141 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
     4201 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
     4261 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     4321 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     4381 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaaaccc
     4441 agctttcttg tacaaagtgg tgataatcga attccgataa tcaacctctg gattacaaaa
     4501 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     4561 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     4621 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     4681 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     4741 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     4801 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     4861 tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc
     4921 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     4981 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     5041 ggatctccct ttgggccgcc tccccgcatc gggaattccc gcggttcgaa ttctaccggg
     5101 taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca
     5161 cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc
     5221 aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga
     5281 agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc
     5341 tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg
     5401 gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg
     5461 ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg
     5521 gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct
     5581 catctccggg cctttcgacc tcacgtggcc accatgaaaa agcctgaact caccgcgacg
     5641 tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg
     5701 gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg
     5761 gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg
     5821 gccgcgctcc cgattccgga agtgcttgac attggggaat ttagcgagag cctgacctat
     5881 tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc
     5941 gctgttctgc agccggtcgc ggaggccatg gatgcgatcg ctgcggccga tcttagccag
     6001 acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat
     6061 ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc
     6121 gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc
     6181 gaagtccggc acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc
     6241 cgcataacag cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc
     6301 gccaacatct tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc
     6361 gagcggaggc atccggagct tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt
     6421 ggtcttgacc aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg
     6481 cagggtcgat gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc
     6541 gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga
     6601 aaccgacgcc ccagcactcg tccgagggca aaggaatagg gtacctttaa gaccaatgac
     6661 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     6721 aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga
     6781 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     6841 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     6901 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca
     6961 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt
     7021 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     7081 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     7141 tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     7201 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     7261 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg
     7321 tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac
     7381 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     7441 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     7501 gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     7561 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     7621 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     7681 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     7741 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     7801 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     7861 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     7921 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg
     7981 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     8041 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     8101 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     8161 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     8221 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     8281 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     8341 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     8401 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     8461 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     8521 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     8581 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     8641 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     8701 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     8761 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     8821 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     8881 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     8941 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     9001 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     9061 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     9121 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     9181 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     9241 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     9301 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     9361 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     9421 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     9481 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     9541 cacgcttccc gaagagagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     9601 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     9661 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     9721 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     9781 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     9841 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     9901 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     9961 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
    10021 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
    10081 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
    10141 gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt
//
