LOCUS       pIVT-R2Tg-RTmut         9540 bp    DNA     circular SYN 23-JAN-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9540)
  AUTHORS   grace
  TITLE     Direct Submission
  JOURNAL   Exported Apr 7, 2025 from SnapGene 8.0.3
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9540
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1..100)
                     /codon_start=1
                     /label=lacI
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.9"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="*NQ*RYTMSQSMPVSLIRPFPAW*TRPATFLRK"
     promoter        complement(101..178)
                     /label=lacI promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             complement(186..465)
                     /codon_start=1
                     /label=TcR (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=23.5"
                     /note="/other=CDS"
                     /translation="DPLRRTHRGRHHRRHRCGCWRLYRRHHRWGRSGSPLRAHERLFRR
                     GYGGRPRGRGTVGRHLLACTIPCGGGAQRPQPTTGLLPNAGVA*GRAS"
     primer_bind     487..579
                     /label=oGE346
     primer_bind     487..505
                     /label=T7
                     /note="ApEinfo_revcolor: #b1ff67"
     misc_feature    487..503
                     /label=min t7
     promoter        487..503
                     /label=T7 promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=89.5"
                     /note="/other=promoter"
     misc_feature    506..557
                     /label=5'UTR
     primer_bind     536..575
                     /label=oGE627
     CDS             558..4100
                     /codon_start=1
                     /label=hR2Tg
                     /label=Translation 9743-13915
                     /translation="MGLEQTGEEKVMVTVPDKNPPCPCCGTRVNSVLNLIEHLKVSHGK
                     RGVCFRCAKCGKENSNYHSVVCHFPKCRGPETEKAPAGEWICEVCNRDFTTKIGLGQHK
                     RLAHPAVRNQERIVASQPKETSNRGAHKRCWTKEEEELLIRLEAQFEGNKNINKLIAEH
                     ITTKTAKQISDKRRLLSRKPAEEPREEPGTCHHTRRAAASLRTEPEMSHHAQAEDRDNG
                     PGRRPLPGRAAAGGRTMDEIRRHPDKGNGQQRPTKQKSEEQLQAYYKKTLEERLSAGAL
                     NTFPRAFKQVMEGRDIKLVINQTAQDCFGCLESISQIRTATRDKKDTVTREKHPKKPFQ
                     KWMKDRAIKKGNYLRFQRLFYLDRGKLAKIILDDIECLSCDIPLSEIYSVFKTRWETTG
                     SFKSLGDFKTYGKADNTAFRELITAKEIEKNVQEMSKGSAPGPDGITLGDVVKMDPEFS
                     RTMEIFNLWLTTGKIPDMVRGCRTVLIPKSSKPDRLKDINNWRPITIGSILLRLFSRIV
                     TARLSKACPLNPRQRGFIRAAGCSENLKLLQTIIWSAKREHRPLGVVFVDIAKAFDTVS
                     HQHIIHALQQREVDPHIVGLVSNMYENISTYITTKRNTHTDKIQIRVGVKQGDPMSPLL
                     FNLAMDPLLCKLEESGKGYHRGQSSITAMAFAAALVLLSDSWENMNTNISILETFCNLT
                     GLKTQGQKCHGFYIKPTKDSYTINDCAAWTINGTPLNMIDPGESEKYLGLQFDPWIGIA
                     RSGLSTKLDFWLQRIDQAPLKPLQKTDILKTYTIPRLIYIADHSEVKTALLETLDQKIR
                     TAVKEWLHLPPCTCDAILYSSTRDGGLGITKLAGLIPSVQARRLHRIAQSSDDTMKCFM
                     EKEKMEQLHKKLWIQAGGDRENIPSIWEAPPSSEPPNNVSTNSEWEAPTQKDKFPKPCN
                     WRKNEFKKWTKLASQGRGIVNFERDKISNHWIQYYRRIPHRKLLTALQLRANVYPTREF
                     LARGRQDQYIKACRHCDADIESCAHIIGNCPVTQDARIKRHNYICELLLEEAKKKDWVV
                     FKEPHIRDSNKELYKPDLIFVKDARALVVDVTVRYEAAKSSLEEAAAEKVRKYKHLETE
                     VRHLTNAKDVTFVGFPLGARGKWHQDNFKLLTELGLSKSRQVKMAETFSTVALFSSVDI
                     VHMFASRARKSMVM"
     misc_feature    561..572
                     /label=start
     misc_feature    816..827
                     /label=ZF
     modified_base   816..827
                     /label=SEVS
     modified_base   939..962
                     /label=AGAHKRCA
     misc_feature    939..962
                     /label=Myb
     misc_feature    complement(2003..2052)
                     /label=R
     misc_feature    2535..2552
                     /label=RT- R
     primer_bind     complement(2540..2572)
                     /label=oGE348
     misc_feature    2552
                     /label=junction 1
                     /label=nonstandard type: Junction
     primer_bind     2553..2586
                     /label=oGE347
     modified_base   2553..2558
                     /label=FAAA
     misc_feature    2553..2558
                     /label=RT
     misc_feature    2559..2564
                     /label=DD>AA mutation for dead RT
     misc_feature    3744..3752
                     /label=EN-
     modified_base   3744..3752
                     /label=KPA
     primer_bind     complement(4077..4128)
                     /label=oGE628
     misc_feature    4101..4399
                     /label=3'UTR FI element
     terminator      4540..4587
                     /label=T7 terminator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=terminator"
     misc_feature    complement(4609..4627)
                     /label=JS_0136
                     /note="ApEinfo_revcolor: #c7b0e3"
     CDS             complement(4610..4714)
                     /codon_start=1
                     /label=TcR (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.8"
                     /note="/other=CDS"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDI"
     misc_feature    complement(4629..4648)
                     /label=JS148
                     /note="ApEinfo_revcolor: #c7b0e3"
     promoter        complement(4762..4790)
                     /label=tet promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     promoter        4904..5008
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             5009..5869
                     /codon_start=1
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     exon            5207..5866
                     /label=Translation 2105-1446
     CDS             5207..5866
                     /label=AmpR
                     /note="ApEinfo_revcolor: #85dae9"
     rep_origin      5964..6646
                     /label=rep_origin
                     /note="ApEinfo_revcolor: #85dae9"
     rep_origin      6040..6628
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     misc_feature    complement(6814..6953)
                     /label=bom
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.3"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     CDS             complement(7055..7243)
                     /codon_start=1
                     /label=rop
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=98.4"
                     /note="/other=CDS"
                     /translation="TKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHAD
                     ELYRSCLARFGDDGENL"
     CDS             7263..7643
                     /codon_start=1
                     /label=YPB1_ECOLX
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=99.2"
                     /note="/match_length=96.2"
                     /note="/other=CDS"
                     /translation="GGFLFMGVMIPMKRERMLTIRVTDDEHARLLERCEGKQLAVWMRR
                     DQRKITQGQCQRFVNTDVGVPQGSQQHPAMQIRNIMVQGADFRVSRLYETRKPKTIHVV
                     AQVADVLQQQSLHVRSRIGDSFC"
     CDS             complement(7885..8509)
                     /codon_start=1
                     /label=TcR (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=52.5"
                     /note="/other=CDS"
                     /translation="STDALESLQPSQLLPVGAGHDYRRRTYDCLLYHATRRTGAGSALG
                     HFRRGPLSLERDDDRPVACGIRNLARPRSSLRHWSRHQTFRREAGHYRRHGGRRAGLRL
                     AGVRDARLDGLPHYDSSRFRRHRDARVAGHAVQAGR*RPSGTASRIARGSYQPNFDHWT
                     ADRHGDLCRLGEHMERVGMDCRRRPIPCLPPRVASRCMEPGHLDL"
     protein_bind    complement(8524..8545)
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=95.5"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     CDS             complement(8558..9540)
                     /codon_start=1
                     /label=lacI
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.9"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="RGKKWKRRWRS*ITFPTAWHNNWRANSRC*LALPPPVWPCTRRRK
                     LSRRLNLAPINWVPAWWCRW*NEAASKPVKRRCTIFSRNASVG*SLTIRWMTRMPLLWK
                     LPALMFRRYFLMSLTRHPSTVLFSPMKTVRDWAWSIWSHWVTSKSRC*RAH*VLSRRVC
                     VWLAGINISLAIKFSR*RNGKATGVPCPVFNKPCKC*MRASFPLRCWLPTIRWRWAQCA
                     PLPSPGCALVRISR*WDTTIPKTAHVISRR*PPSNRIFACWGKPAWTACCNSLRARR*R
                     AISCCPSHW*KEKPPWRPIRKPPLPARWPIH*CSWHDRFPDWKAGS"
ORIGIN
        1 ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac
       61 cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac
      121 tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtccg
      181 ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg
      241 aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt
      301 cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag
      361 tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct
      421 gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg
      481 cgaaattaat acgactcact ataaggaata aactagtatt cttctggtcc ccacagactc
      541 agagagaacc cgccaccatg ggcctggaac agaccggcga agagaaagtg atggtcaccg
      601 tgcctgacaa gaaccctcct tgtccttgct gcggcaccag agtgaactcc gtgctgaacc
      661 tgatcgagca cctgaaggtg tcccacggca aacggggcgt gtgcttcaga tgtgccaagt
      721 gcggcaaaga gaacagcaac taccacagcg tcgtgtgtca cttccccaag tgcagaggcc
      781 ccgagacaga aaaagctcct gccggcgagt ggatctgcga agtgtgcaac agagacttca
      841 ccaccaagat cggcctgggc cagcacaaga gactggctca tcctgccgtg cggaatcaag
      901 agcggattgt ggccagccag cctaaagaga caagcaacag aggcgcccac aagcggtgct
      961 ggaccaaaga ggaagaggaa ctgctgatcc ggctggaagc ccagttcgag ggcaacaaga
     1021 acatcaacaa gctgatcgcc gagcacatca ccacaaagac cgccaagcag atcagcgaca
     1081 agcggaggct gctgagcaga aagcctgccg aggaacccag agaggaaccc ggaacctgtc
     1141 accacacaag aagggccgct gccagcctga gaacagagcc tgagatgtct catcacgctc
     1201 aggccgagga cagagacaat ggccctggaa gaaggcctct gcctggtaga gctgctgctg
     1261 gcggcagaac catggacgag attagacggc accccgacaa aggcaacggc cagcagaggc
     1321 caacaaagca gaagtccgag gaacagctgc aggcctacta caagaaaaca ctggaagaga
     1381 gactgagcgc tggcgccctg aacacctttc ctagagcctt caagcaagtg atggaaggcc
     1441 gggacatcaa gctggtcatc aaccagacag cccaggactg cttcggctgc ctggaatcca
     1501 tcagccagat cagaaccgcc accagagaca agaaagacac cgtgaccaga gagaagcacc
     1561 ccaagaaacc cttccagaaa tggatgaagg accgcgccat caagaaggga aactacctgc
     1621 ggttccagcg gctgttctac ctggacagag gcaagctggc caagatcatc ctggacgaca
     1681 tcgagtgcct gagctgcgac atccctctga gcgagatcta cagcgtgttc aagaccagat
     1741 gggagacaac cggcagcttc aagagcctgg gcgacttcaa gacatacggc aaggccgaca
     1801 acaccgcctt cagagagctg atcaccgcca aagaaatcga gaagaacgtg caagagatga
     1861 gcaagggcag cgcccctgga cctgatggaa tcacactggg cgacgtggtc aagatggacc
     1921 ccgagtttag ccggaccatg gaaatcttca acctgtggct gaccaccggc aagatccccg
     1981 atatggttcg aggctgcaga accgtgctga tccccaagag cagcaagccc gacagactga
     2041 aggatatcaa caactggcgg cccatcacca tcggcagcat tctgctgagg ctgttcagca
     2101 ggatcgtgac cgccagactg agcaaggcct gtcctctgaa ccctcggcag agaggcttta
     2161 tcagagccgc cggatgtagc gagaacctga agctgctgca gaccatcatt tggagcgcca
     2221 agagagagca cagacccctg ggcgtcgtgt tcgtggatat cgccaaggcc ttcgacaccg
     2281 tgtctcacca gcacatcatt cacgccctgc agcagagaga ggtggaccct catatcgtgg
     2341 gcctcgtgtc caatatgtac gagaacatca gcacctacat taccaccaag cggaacaccc
     2401 acaccgataa gatccagatt agagtgggcg tgaagcaggg cgaccctatg agccctctgc
     2461 tgttcaatct ggccatggat ccactgctgt gcaagctgga agagtccggc aagggctatc
     2521 acagaggcca gtctagcatc acagccatgg ccttcgccgc tgcactggtg ctgctgtctg
     2581 acagctggga gaacatgaac accaacatct ctatcctgga aaccttctgc aacctgaccg
     2641 gcctgaaaac ccagggacag aagtgccacg gcttctacat caagcccacc aaggacagct
     2701 acaccatcaa cgattgtgcc gcctggacca tcaatggcac ccctctgaat atgatcgacc
     2761 ccggcgagag cgagaagtac ctgggcctgc aattcgaccc ctggatcgga attgccagat
     2821 ccggcctgtc caccaagctg gatttctggc tgcagcggat cgatcaggcc ccactgaagc
     2881 ctctgcagaa aaccgacatc ctcaagacct acacaatccc caggctgatc tatatcgccg
     2941 accacagcga agtgaaaaca gccctgctgg aaaccctgga ccagaaaatc cggaccgccg
     3001 tgaaagagtg gctgcatctg cctccatgca cctgtgacgc catcctgtac tctagcacca
     3061 gagatggcgg cctgggaatc acaaaactgg ccggactgat cccctccgtg caggctagaa
     3121 ggctgcacag aattgcccag agcagcgacg acaccatgaa gtgctttatg gaaaaagaaa
     3181 agatggaaca gctccacaag aagctgtgga tccaggctgg cggcgacaga gagaacatcc
     3241 cctctatttg ggaagcccct cctagcagcg agcctcctaa caacgtgtcc acaaactccg
     3301 agtgggaagc tcccacacag aaggacaagt tccccaagcc ttgcaattgg cggaagaacg
     3361 agttcaagaa gtggaccaag ctcgccagcc aaggcagggg catcgtgaac ttcgagcggg
     3421 acaagatcag caaccactgg attcagtact accggcggat ccctcacaga aagctgctga
     3481 cagcactgca gctgcgggcc aacgtgtacc ctaccagaga attcctggcc agaggacggc
     3541 aggaccagta catcaaagcc tgcagacact gcgacgccga tatcgagtct tgcgcccaca
     3601 tcatcggcaa ctgccccgtg acacaggatg cccggatcaa gcggcacaac tacatctgcg
     3661 aactgctgct cgaggaagcc aagaaaaagg actgggtcgt gttcaaagag ccccacatcc
     3721 gggacagcaa caaagagctg tacaagcccg atctgatctt cgtgaaggac gctagagccc
     3781 tggtggtgga cgtgaccgtt agatatgagg ccgccaagtc tagtctggaa gaggctgccg
     3841 ctgagaaagt gcggaagtac aagcacctcg aaaccgaagt ccggcacctg accaacgcca
     3901 aggatgtgac cttcgtgggc tttccactgg gcgccagagg aaagtggcac caggacaact
     3961 tcaaactgct gactgagctg ggcctgagca agtcccggca agtgaagatg gccgagacat
     4021 tcagcacagt ggccctgttc agctccgtgg acatcgtgca catgttcgcc tccagagcca
     4081 gaaagtctat ggtcatgtga tgactcgagc tggtactgca tgcacgcaat gctagctgcc
     4141 cctttcccgt cctgggtacc ccgagtctcc cccgacctcg ggtcccaggt atgctcccac
     4201 ctccacctgc cccactcacc acctctgcta gttccagaca cctcccaagc acgcagcaat
     4261 gcagctcaaa acgcttagcc tagccacacc cccacgggaa acagcagtga ttaaccttta
     4321 gcaataaacg aaagtttaac taagctatac taaccccagg gttggtcaat ttcgtgccag
     4381 ccacaccctg gagctagcaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaag catatgacta
     4441 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     4501 aaaaaaaaat tataagctgc tgccaccgct gagcaataac tagcataacc ccttggggcc
     4561 tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatcccgca
     4621 agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc
     4681 cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat
     4741 ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat
     4801 tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     4861 atggtttctt agacgtcagg tggcactttt cgggggaaat gtgcgcggaa cccctatttg
     4921 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     4981 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     5041 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     5101 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     5161 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     5221 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
     5281 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     5341 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     5401 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     5461 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     5521 accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact
     5581 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     5641 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     5701 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     5761 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     5821 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     5881 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     5941 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     6001 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     6061 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     6121 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     6181 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     6241 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     6301 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     6361 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     6421 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     6481 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     6541 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     6601 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     6661 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     6721 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     6781 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     6841 tctgtgcggt atttcacacc gcaatggtgc actctcagta caatctgctc tgatgccgca
     6901 tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac
     6961 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     7021 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     7081 aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt
     7141 ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc
     7201 tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtaactgat gcctccgtgt
     7261 aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga
     7321 tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg
     7381 cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta
     7441 atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca
     7501 taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga
     7561 ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct
     7621 cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc
     7681 tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc
     7741 gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt
     7801 gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt
     7861 tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg
     7921 caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca
     7981 tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccaatga tcgaagttag
     8041 gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct
     8101 ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat
     8161 ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc
     8221 cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac
     8281 gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt
     8341 cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc
     8401 tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc
     8461 ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc
     8521 ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     8581 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     8641 tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct
     8701 tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc
     8761 gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt
     8821 cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca
     8881 ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat
     8941 tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg
     9001 ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg
     9061 ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca
     9121 gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg
     9181 tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa
     9241 tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa
     9301 gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca
     9361 cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt
     9421 gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt
     9481 gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg
//
