LOCUS       pSL820_cr-ki_Ex8        8409 bp    DNA     circular SYN 18-FEB-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 8409)
  AUTHORS   MRC Lab University of Dundee Allison Bridges
  TITLE     Direct Submission
  JOURNAL   Exported Apr 9, 2025 from SnapGene 8.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8409
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(751..769)
                     /label=oSL802_p_rev
     CDS             772..864
                     /codon_start=1
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.6"
                     /note="/other=CDS"
                     /translation="APNTQTASPRALADSLMQLARQVSRLESGQ"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     975..992
                     /label=M13 Rev(GW)
                     /note="Specific for Gateway vectors"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     977..995
                     /label=M13 Rev
                     /note="Common to many plasmids"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    1019..1345
                     /label=Synthetic 820_US
     misc_feature    1019..1043
                     /label=Overlap_1
     primer_bind     complement(1019..1043)
                     /label=oSL802_p_rev_60
     primer_bind     1019..1043
                     /label=Overlap_1_fwd
     primer_bind     1020..1044
                     /label=820b_fwd_rbcs2_O1
     misc_feature    1044..1051
                     /standard_name="BspQI"
                     /label=BspQI
     CDS             1052..1236
                     /codon_start=3
                     /label=Exon 8
                     /translation="GFKKLNFLLQSPPFPPETFANLLLLYCKPSHGFYDLARDVLRENP
                     QYAGKLLSPDLYDYLQ"
     misc_feature    1052..1101
                     /label=50bp upstream
     misc_feature    join(1096..1098,1102..1118)
                     /label=CRISPR target
     misc_feature    1102..1151
                     /label=50 bp downstream
     misc_feature    1107..1113
                     /label=Edited codons to mismatch and avoid recutting
     misc_feature    1156..1158
                     /label=CFAP36 binding
     misc_feature    1165..1167
                     /label=A331R
     misc_feature    1165..1167
                     /label=CFAP36 binding
     misc_feature    1177..1179
                     /label=A335R
     misc_feature    1177..1179
                     /label=CFAP36 binding
     primer_bind     complement(1214..1293)
                     /label=820_gap_rev
     primer_bind     complement(1216..1237)
                     /label=Primer 3
     misc_feature    1222..1224
                     /label=CFAP36 binding
     primer_bind     complement(1271..1345)
                     /label=820_gap2_rev
     primer_bind     1319..1394
                     /label=820_gap2_fwd
     primer_bind     1319..1346
                     /label=oSL820_Ex8_US_fwd
     primer_bind     complement(1320..1345)
                     /label=oSL820_Ex8_US_rev
     primer_bind     1372..1449
                     /label=820_gap_fwd
     CDS             1580..1774
                     /codon_start=1
                     /label=Exon 9
                     /translation="AAIGRYKSPEEAFRRFDELATRHVEQLRRLTKQIQDARIARDNDA
                     IKRAINEYDEALEAYIPGLM"
     misc_feature    1586..1588
                     /label=CFAP36 binding
     CDS             2114..2268
                     /codon_start=1
                     /label=Exon 10
                     /translation="AMASIYWDMELYSNVEKIFRQSAEFCSEHEVWKLNVAHTFFMQDN
                     HYKEAI"
     CDS             2510..2609
                     /codon_start=2
                     /label=Exon 11
                     /translation="YYEPVVKKNADNLLGVTAIVLANLCVSYIMTSQ"
     CDS             2947..3155
                     /codon_start=1
                     /label=Exon 12
                     /translation="NEEAEELMRKVEKEEERSSMQDPDKPCFHLCIINLVIGTLYCAKG
                     NYEFGVSRIIKSLEPYDKKLETDT"
     CDS             3373..3595
                     /codon_start=2
                     /label=Exon 13
                     /translation="YYAKRCFLALIENLAKHMIVLKDSSFTEIMAFLNEAEKHGKDIRV
                     VFNEGKHQSRTIASEARMLKKMFLKLRD"
     primer_bind     3501..3530
                     /label=847_dePAM_fwd
     primer_bind     complement(3501..3530)
                     /label=847_dePAM_rev
     primer_bind     complement(3516..3551)
                     /label=847_DS_rev
     primer_bind     3532..3565
                     /label=847_DS_fwd
     3'UTR           3596..4691
     primer_bind     complement(4668..4715)
                     /label=oSL820_IFT70_3UTR_rev_flank
     primer_bind     complement(4668..4691)
                     /label=oSL820_IFT70_3UTR_rev
     primer_bind     complement(4668..4691)
                     /label=oSL820a_rev_I70_O4
     primer_bind     4687..4715
                     /label=820b_fwd_rbcs2_O1
     primer_bind     4692..4715
                     /label=crBSDr_fwd_64
     promoter        4712..4927
                     /standard_name="rbcS2-P"
                     /label=rbcS2-P promoter
     intron          4936..5080
                     /locus_tag="rbcS2 intron1"
                     /standard_name="rbcS2-i1"
                     /label=rbcS2-i1
     CDS             5090..6112
                     /standard_name="AadA"
                     /label=AadA
     primer_bind     5141..5151
                     /label=crBSDr_rev_65
     3'UTR           6117..6350
                     /locus_tag="rbcS2 3' UTR"
                     /standard_name="rbcS2-T"
                     /label=rbcS2-T
     misc_feature    6329..6628
                     /label=Synthetic 820_DS
     misc_feature    6329..6355
                     /label=Overlap_3
     primer_bind     complement(6329..6350)
                     /label=crBSDr_rev_65
     primer_bind     6329..6350
                     /label=pSL810_p1_fwd_65
     primer_bind     6329..6348
                     /label=Overlap_3_fwd
     primer_bind     complement(6335..6355)
                     /label=847_DS_rev
     misc_feature    6356..6405
                     /label=50 bp downstream
     CDS             6356..6405
                     /codon_start=3
                     /label=Exon 8
                     /translation="ETFANLLLLYCKPSHG"
     misc_feature    6356..6372
                     /label=CRISPR target
     primer_bind     6402..6434
                     /label=847_DS_fwd
     misc_feature    complement(6406..6412)
                     /standard_name="BspQI"
                     /label=BspQI
     misc_feature    6442..6466
                     /standard_name="Ligation"
                     /label=Ligation
                     /note="Geneious type: ligation"
     misc_feature    6502..6601
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=filler_100bp
                     /note="confers resistance to gentamycin"
     primer_bind     complement(6601..6627)
                     /label=oSL820a_rev_I70_O4
     misc_feature    6602..6628
                     /label=Overlap_4
     primer_bind     6602..6627
                     /label=oSL802_p_fwd
     primer_bind     complement(6604..6628)
                     /label=Overlap_4_rev
     promoter        complement(6607..6625)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             6633..6788
                     /codon_start=1
                     /label=lacZ-alpha (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=89.7"
                     /note="/other=CDS"
                     /translation="SLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQ
                     LRSLNGE"
     primer_bind     complement(6635..6651)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      6793..7248
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6901..6920)
                     /label=SV40/FRT_p5_rev
     primer_bind     complement(6902..6921)
                     /label=oSL17_SV40/FRT_rev
     promoter        7274..7378
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7379..7447,7448..8239)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tgggctcttc tgcggcttca agaagctcaa cttcctgctg
     1081 cagagcccgc ccttcccgcc cgagacgttc gcgaacctgc tgctgctgta ctgcaagccc
     1141 agccacggct tctacgacct ggcgcgcgac gtgcttcgcg agaacccgca gtacgccggc
     1201 aagctgctca gccccgacct gtacgactac ctgcaggtgc ggggggcggg gggaggcggg
     1261 tagtagatac cagcccaaag tcaaccgaac ctggactggg gaccggggac tgtaaccacc
     1321 agcccaagct aaattgaacc cattgcaata gggcgaggag gagagcgtgg cctatgcttt
     1381 actacgcggg ggggggcgcg gtgtgtgggt cggatgtgtc agatgtgctg ggtgcgggcg
     1441 gctagagagt ggttttgagg ctcgatgttg aagcagcggt gggtaggact cttctgagag
     1501 tactggaaat gcccacagga gtttctgtct gatctggtgt cttcttcttt ccctaccatg
     1561 cgccccctgc ttccgccagg ccgccatcgg gcgctacaag agtcccgagg aggcgttccg
     1621 gcgcttcgac gagctggcca cccgccacgt ggagcagctg cggcggctga ccaagcagat
     1681 ccaggacgcc cgcatcgcgc gcgacaacga cgccatcaag cgcgccatca acgagtatga
     1741 cgaggcgctg gaggcctaca tccccggact catggtgcgt gggctgggct ggggtgggcg
     1801 gggctgcgga gggctggggt gcggagggag ggaggaaggg agggtggatg ggctgtgcga
     1861 ggggggtttg tgtgcgaggg gaattgtgcg ttggggtggc ctcgtgcggt tggctggggg
     1921 cgcggttgag caggctgacc cagctggctg ccggaaagtc ttttttacgc taccttcctg
     1981 gctattagcc ccatgattcc tagtttggtt gggacttaac caacaactac ccctgccccg
     2041 ctcccaccag ccccaccatg cctgatttcc tcccctcccc tcctcatccc cttctttcct
     2101 gccccgccct caggccatgg cgagcatcta ctgggacatg gagctgtaca gcaacgtgga
     2161 gaagatcttc cgccagtcgg ccgagttctg ctccgagcac gaggtgtgga agctcaacgt
     2221 ggcgcacacc ttcttcatgc aggacaacca ctacaaggag gccatcaggt gggggcaggc
     2281 agcgggggtg ggggcagcgg ggctgggagg ctggcgatcg cgaccgctgc gggggaggcg
     2341 atggggcggc agtcagcgtc ctttgccctg tgctgtgatt gcaactgtaa ctggccagtc
     2401 gtagagttcg caaccgagcc cctcgcctcg ccccatcgcg ccgctcactt ctgtgtggat
     2461 gtggatgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt actgtgcagg tactacgagc
     2521 ctgtggtgaa gaagaacgcg gacaacctgc tgggcgtcac cgccatcgtg ctcgccaacc
     2581 tgtgcgtgtc ctacatcatg acaagccagg tgcgtgcgtg ctggagcagc ctaagggaca
     2641 ccgcggggtt cggggtgcgg gtatgtgcct gcgtttgtgt gactgcgcat gtgtttgagg
     2701 gcacacggaa ggaaggagga gacacgcaag gctgtgtatg cgatgcggcg tgcatgtgta
     2761 tacgaagttt gggcggcgct gcaatatgtg gcaagcccta tctcggttag acctccgtat
     2821 cgcagctaga acaagcgccc cgacgtctgc cagcgacctt cccctgacat gcacagcccc
     2881 tgaccccctc caaccccaac tccgcaccct cctcccgttc ccactcccgc tctccgctca
     2941 acgcagaacg aggaggctga ggagctgatg cgcaaggtgg agaaggagga ggagcgcagc
     3001 tccatgcagg accccgacaa gccctgcttc cacctgtgca tcattaacct ggtcatcggc
     3061 acgctgtact gcgccaaggg caactacgag ttcggcgtgt cgcgcatcat caagtcgctg
     3121 gagccgtacg acaagaagct ggagaccgac acctggtgcg tacggcacag caaagtactg
     3181 ccgtgttact gctgtaataa tgtacagcac ggccgaggag gcagggatgg catggcatca
     3241 ggcgggcagg gtcagcgcga ctggggccac ggatgctacc gtagacgcgg gtatgcccac
     3301 cctctccact ctttccgtca tcttacccgg ctccacctct ccccgtgtcc atccgccggc
     3361 ccacttgcgc aggtactacg ccaagcgctg cttcctggcg ctgatcgaga acttggccaa
     3421 gcacatgatc gtgcttaagg acagcagctt cacggagatc atggccttcc tcaacgaggc
     3481 ggagaagcac ggcaaggaca tccgcgtggt gttcaacgag ggcaagcacc agagccgcac
     3541 cattgcgtcg gaggcgcgca tgctcaagaa gatgttcctc aagctgcgcg actaaggcgc
     3601 taggagaatg gggagcggga gcaggcggct ggagcgggcg cggagaaggg gcggcgggct
     3661 tggtgggcgt ggagttgcgt ggatttgcgc gtcctgcttg gtggtggggg cagcggcgtg
     3721 gcgtgtcaag gcagtggcgc gctggaggcg cgtcaatgcg ccccaccacg gagcctggcg
     3781 gaggtgtggg cggggtaact tgggcgcatg attgaagaga atgggctgct gaaagattgc
     3841 agtcacgcat gctggaccgg ttgggaggct acaaggcgaa acagtggcca ggcgggcgat
     3901 tgcctgcctg ataatgtaca tacctttggc agtggtgcac gtggtgagta taaagcaagt
     3961 tacagtggcg ggcgaggaca aatgtgtgct caggttaggg cgcattccgg gaaggaggcg
     4021 atgtaaggag taggaactgg cgcatgtggc agtgtgggta cgccacgcac acgagccatg
     4081 ccaggctggg tccgagggct caggtatgca ggacagcgcc gtcgcgcatt ggtttgcagg
     4141 ccagtgcggg tttgcatgcg agcgcatgac aggccatctc aggggcgcat ctctgcgtgc
     4201 ttgcgcgctg ggattgtccc ttgtgtagaa tgcaggccgt gtgggaacgt ggcgagcgcg
     4261 agacagcgag agtgcctcgg ataagtcgga ttgcccccga tcgactggac tgagacagtc
     4321 cgcgggcgtg ggtgggctgt gaactggagg ctcgagcccc cggcccgaca cggtgcgcac
     4381 gggccaagca gggccaagtt gacgtatatc cgtagttccg tatggatgtg acgagccggc
     4441 gcaacactcc ttgcaatgct gcaaataagg cgagcaatgt ggttatgtgg agtcagctgg
     4501 ccccacgctg gtcgccccca attcctgcgg cgccacacgc atagctttgg acacgcgctg
     4561 gacacgatac ggtaccccgg acaactttcc gctcgcctgc aagctccgta tgcccgttca
     4621 tcacctgcgc aaccgtcttg gttcacgaag ccgtgatgcg atgacccgcc agtgtgaaat
     4681 gtcacggctg ggatcccatc aagcttgcat gccgggcgcg ccagaaggag cgcagccaaa
     4741 ccaggatgat gtttgatggg gtatttgagc acttgcaacc cttatccgga agccccctgg
     4801 cccacaaagg ctaggcgcca atgcaagcag ttcgcatgca gcccctggag cggtgccctc
     4861 ctgataaacc ggccaggggg cctatgttct ttactttttt acaagagaag tcactcaaca
     4921 tcttaaaatg gccaggtgag tcgacgagca agcccggcgg atcaggcagc gtgcttgcag
     4981 atttgacttg caacgcccgc attgtgtcga cgaaggcttt tggctcctct gtcgctgtct
     5041 caagcagcat ctaaccctgc gtcgccgttt ccatttgcag cgcgacgtca tggccatggc
     5101 ggccgtgatc gccaagtcga gcgtgagcgc cgccgtggcc cgccccgccc gctccagcgt
     5161 gcgccccatg gccgccttga agcccgccgt gaaggccgcg cccgtggccg cgcccgccca
     5221 agccaaccag caattgatgg ccatgcgcac cccggaggag ctgtccaacc tgattaagga
     5281 tctgatcgag cagtacactc ccgaggtcaa gatgtcgatg gctcgggagg ccgtgattgc
     5341 ggaggtctcg acccagctga gcgaagtcgt gggcgtcatc gagcgccacc tggagcccac
     5401 cctgctggcc gtgcacctgt acggctccgc cgtggacggg ggcctgaagc cccactcgga
     5461 catcgacctg ctcgtgaccg tgaccgtgcg cctggacgag actactcgcc gggctctcat
     5521 caacgacctg ctggaaacga gcgcgtcgcc tggcgagtcg gagatcctgc gcgccgtgga
     5581 agtcaccatc gtcgtgcatg acgacattat cccctggcgc tacccggcca agcgcgagct
     5641 gcaattcggc gagtggcagc gcaacgacat cctggccggc atcttcgagc ccgcgaccat
     5701 tgacatcgac ctggcgatcc tcctgacgaa ggcccgcgag cactccgtgg cgctcgtcgg
     5761 cccggcggcg gaggagctgt ttgaccccgt gccggagcag gacctgttcg aggctctgaa
     5821 cgagaccctg acgctgtgga actcccctcc ggattgggcc ggcgacgagc ggaacgtcgt
     5881 gctgaccctg agccgcattt ggtattcggc ggtcaccggc aagatcgccc ccaaggacgt
     5941 ggcggcggac tgggccatgg agcggctgcc ggcgcaatac cagcccgtga tcctggaggc
     6001 ccggcaagcc tacctcgggc aggaggagga ccgcctggcg agccgggcgg accagctgga
     6061 ggagttcgtg cactacgtca agggcgagat cacgaaggtc gtgggcaagt aaatccccgc
     6121 tccgtgtaaa tggaggcgct cgttgatctg agccttgccc cctgacgaac ggcggtggat
     6181 ggaagatact gctctcaagt gctgaagcgg tagcttagct ccccgtttcg tgctgatcag
     6241 tctttttcaa cacgtaaaaa gcggaggagt tttgcaattt tgttggttgt aacgatcctc
     6301 cgttgatttt ggcctctttc tccatgggcg ggctgggcgt atttgaagcg ggtacgagac
     6361 ctttgccaac ctgctgctgc tgtactgcaa gcccagccac ggcttgaaga gccaaacgag
     6421 cacttataca tacgcgacta ttctgccgct atacataacc actcaggagc tctatgtgac
     6481 aatgcttccg tgacgccgcg aggtacttgg gtcgatatca aagtgcatca cttcttcccg
     6541 tatgcccaac tttgtataga gagccactgc gggatcgtca ccgtaatctg cttgcacgta
     6601 gttcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg
     6661 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     6721 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     6781 atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     6841 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     6901 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     6961 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     7021 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     7081 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     7141 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     7201 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt
     7261 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     7321 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     7381 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     7441 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     7501 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     7561 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     7621 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     7681 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     7741 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     7801 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     7861 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     7921 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     7981 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     8041 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     8101 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     8161 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     8221 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     8281 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     8341 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     8401 aggatcttc
//
