LOCUS       pRK5_mCherry-P2A        9599 bp    DNA     circular SYN 13-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9599)
  TITLE     Spatial spread of Ras activity
REFERENCE   2  (bases 1 to 9599)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 13, 2025 from SnapGene 8.0.2
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pRK5_mCherry-P2AT2A-GFP-NUP98-DDX10
FEATURES             Location/Qualifiers
     source          1..9599
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        14..393
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        394..597
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     548..568
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     594..618
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        828..846
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     828..845
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     902..971
                     /label=HN181
     regulatory      933..941
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             939..1646
                     /codon_start=1
                     /label=mCherry (full)
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1258..1278)
                     /label=backbone_R
     CDS             1656..1712
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(1693..1727)
                     /label=HN870
     CDS             1722..1775
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     1752..1796
                     /label=HN871
     primer_bind     complement(1756..1799)
                     /label=HN870
     misc_feature    1756..1775
                     /label=Feature 29
     CDS             join(1776..1778,1779..1781,1782..2492)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     regulatory      1776..1779
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1821..1842)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2082..2101)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2429..2450
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     2446..2529
                     /label=Fragment 1.FOR
     primer_bind     complement(2473..2492)
                     /label=mEGFP_XhoI_re
     CDS             2502..3909
                     /codon_start=1
                     /label=NUP98
                     /translation="MFNKSFGTPFGGGTGGFGTTSTFGQNTGFGTTSGGAFGTSAFGSS
                     NNTGGLFGNSQTKPGGLFGTSSFSQPATSTSTGFGFGTSTGTANTLFGTASTGTSLFSS
                     QNNAFAQNKPTGFGNFGTSTSSGGLFGTTNTTSNPFGSTSGSLFGPSSFTAAPTGTTIK
                     FNPPTGTDTMVKAGVSTNISTKHQCITAMKEYESKSLEELRLEDYQANRKGPQNQVGAG
                     TTTGLFGSSPATSSATGLFSSSTTNSGFAYGQNKTAFGTSTTGFGTNPGGLFGQQNQQT
                     TSLFSKPFGQATTTQNTGFSFGNTSTIGQPSTNTMGLFGVTQASQPGGLFGTATNTSTG
                     TAFGTGTGLFGQTNTGFGAVGSTLFGNNKLTTFGSSTTSAPSFGTTSGGLFGFGTNTSG
                     NSIFGSKPAPGTLGTGLGAGFGTALGAGQASLFGNNQPKIGGPLGTGAFGAPGFNTTTA
                     TLGFGAPQAPV"
     primer_bind     3890..3942
                     /label=Fragment 2.FOR
     primer_bind     complement(3894..3929)
                     /label=Fragment 1.REV
     CDS             3910..5876
                     /codon_start=3
                     /label=DDX10
                     /translation="LDEADRILDMGFADTMNAVIENLPKKRQTLLFSATQTKSVKDLAR
                     LSLKNPEYVWVHEKAKYSTPATLEQNYIVCELQQKISVLYSFLRSHLKKKSIVFFSSCK
                     EVQYLYRVFCRLRPGVSILALHGRQQQMRRMEVYNEFVRKRAAVLFATDIAARGLDFPA
                     VNWVLQFDCPEDANTYIHRAGRTARYKEDGEALLILLPSEKAMVQQLLQKKVPVKEIKI
                     NPEKLIDVQKKLESILAQDQDLKERAQRCFVSYVRSVYLMKDKEVFDVSKLPIPEYALS
                     LGLAVAPRVRFLQKMQKQPTKELVRSQADKVIEPRAPSLTNDEVEEFRAYFNEKMSILQ
                     KGGKRLEGTEHRQDNDTGNEEQEEEEDDEEEMEEKLAKAKGSQAPSLPNTSEAQKIKEV
                     PTQFLDRDEEEEDADFLKVKRHNVFGLDLKDEKTLQKKEPSKSSIKKKMTKVAEAKKVM
                     KRNFKVNKKITFTDEGELVQQWPQMQKSAIKDAEEDDDTGGINLHKAKERLQEEDKFDK
                     EEYRKKIKAKHREKRLKEREARREANKRQAKAKDEEEAFLDWSDDDDDDDDGFDPSTLP
                     DPDKYRSSEDSDSEDMENKISDTKKKQGMKKRSNSEVEDVGPTSHNRKKARWDTLEPLD
                     TGLSLAEDEELVLHLLRSQS"
     CDS             complement(5590..5616)
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     primer_bind     complement(5845..5903)
                     /label=HN932
     misc_feature    5845..5876
                     /label=Feature 27
     misc_feature    5877..5879
                     /label=Feature 27
     regulatory      5899..5908
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    5912..6046
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5949..5968)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6003..6022
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        6115..6472
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     complement(6138..6158)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6323..6458
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6385..6404
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(6408..6419)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(6492..6509)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6492..6508)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6501..6523)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6721..7176
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6808..6827)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7018..7039
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7193..7212)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7312..7334
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7372..7390)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7458..7562
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7563..7631,7632..8423)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7781..7800)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8594..9182
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9083..9102
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9336..9353
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        join(9506..9511,9512..9529,9530..9536)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9544..9560
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9549..9571
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9568..9584
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9568..9584
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ttcgagctcg cccgacattg attattgact agttattaat agtaatcaat tacggggtca
       61 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      121 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      181 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      241 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      301 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      361 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
      421 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
      481 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
      541 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt
      601 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
      661 caccgggacc gatccagcct ccgcggccgg gaacggtgca ttggaacgcg gattccccgt
      721 gccaagagtg acgtaagtac cgcctataga gtctataggc ccaccccctt ggcttcgtta
      781 gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt taggtgacac
      841 tatagaataa catccacttt gcctttctct ccacaggtgt ccactcccag gtccaactgc
      901 acctcggttc tatcgattga attcgtaccg gtgccaccat ggtttccaaa ggagaggagg
      961 ataatatggc tataattaaa gagtttatgc ggttcaaggt gcatatggag ggatctgtga
     1021 acggtcacga gttcgaaatc gagggcgaag gcgaggggcg accctatgaa gggacacaga
     1081 cggccaaact gaaggtgacc aagggtggcc ccctgccttt cgcctgggac atcctgtcac
     1141 cccagtttat gtacggctct aaggcttacg ttaagcaccc tgccgatatt ccggactact
     1201 tgaaactgag cttccctgaa gggttcaagt gggaacgggt aatgaatttt gaggatggcg
     1261 gtgtagtcac agttacccag gacagctctc ttcaggacgg agaatttatc tataaggtta
     1321 aacttcgggg cactaacttc ccatcagacg gccccgtgat gcagaagaaa actatgggct
     1381 gggaagccag ctcagaacgc atgtatcccg aggacggagc actgaaggga gaaatcaagc
     1441 agcgactcaa gctgaaggat gggggacatt atgatgctga ggtcaaaacc acctataagg
     1501 ccaagaaacc cgttcagctt cctggggcat acaacgtgaa tatcaaactg gatattacat
     1561 cccataacga agactatacc atcgtggaac agtacgagcg ggccgagggc agacatagca
     1621 cagggggcat ggatgaattg tacaagggaa gcggagctac taacttcagc ctgctgaagc
     1681 aggctggaga cgtggaggag aaccctggac ctggaagcgg agagggcaga ggaagtcttc
     1741 taacatgcgg tgacgtggag gagaatcccg gcccaatggt gagcaagggc gaggagctgt
     1801 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
     1861 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
     1921 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg
     1981 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     2041 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
     2101 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
     2161 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
     2221 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc
     2281 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
     2341 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccaagctga
     2401 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     2461 ggatcactct cggcatggac gagctgtaca agaccggcgg catgtttaac aaatcatttg
     2521 gaacaccctt tgggggtggc acaggtggct ttggcacaac ttcaacattt ggacagaata
     2581 ctggctttgg cactactagt ggaggggcat ttggaacatc tgcatttggt tctagcaaca
     2641 atactggagg cctctttgga aattcacaga ctaaaccagg aggattgttt ggaaccagtt
     2701 catttagcca gccagctacc tccacaagca ctggctttgg gtttggtacg tcaacaggaa
     2761 cagcaaatac cttgtttgga actgcaagca cagggaccag tctcttctca tcccaaaaca
     2821 atgcctttgc acaaaataaa ccaactggct ttggcaattt tggaaccagt actagcagtg
     2881 gaggactctt tggaaccaca aataccacct ctaatccttt tggcagcaca tctggctccc
     2941 tctttgggcc aagtagtttt acagctgctc ctactgggac tactattaaa tttaaccctc
     3001 caactggtac agatactatg gtcaaagctg gagttagcac taacataagt accaagcacc
     3061 agtgtattac tgctatgaaa gaatatgaaa gcaagtcact agaggaactt cgtttagagg
     3121 attatcaggc taacaggaag ggcccacaga accaggtggg agcaggtacc acaactggct
     3181 tgtttgggtc ttctccagcc acttccagcg caacaggact cttcagctcc tccaccacta
     3241 attcaggctt tgcatatggt cagaacaaaa ctgcctttgg aactagtaca actggatttg
     3301 gaacaaatcc aggtggtctc tttggccaac agaatcagca gactaccagc ctcttcagca
     3361 aaccatttgg ccaggctaca accacccaga acactggctt ttcctttggt aataccagca
     3421 ccataggaca gccaagcacc aacaccatgg gattatttgg agtaacccaa gcctcacagc
     3481 ctggaggtct ttttgggaca gctacaaaca ccagcactgg gacagcattt ggaacaggaa
     3541 caggtctctt tgggcagacc aatactggat ttggtgctgt tggttcgacc ctgtttggca
     3601 ataacaagct tactacattt ggaagcagca caaccagtgc accttcattt ggtacaacca
     3661 gtggcgggct ctttggtttt ggcacaaata ccagtgggaa tagtattttt ggaagtaaac
     3721 cagcacctgg gactcttgga actgggcttg gtgcaggatt tggaacagct cttggtgctg
     3781 gacaggcatc tttgtttggg aacaaccaac ctaagattgg agggcctctt ggtacaggag
     3841 cctttggggc ccctggattt aatactacga cagccacttt gggctttgga gccccccagg
     3901 ccccagtagt tcttgatgaa gcagatagaa tcttggatat gggctttgct gataccatga
     3961 atgctgttat tgaaaatctc cccaagaaac gtcagacttt acttttctca gcaacacaaa
     4021 ctaaatctgt aaaggacctt gcacgcttga gtttgaaaaa ccctgagtat gtctgggttc
     4081 atgaaaaagc aaaatatagc acccctgcca ctttggaaca gaactacata gtctgtgagc
     4141 tgcagcaaaa aataagtgtg ctgtattcct ttttgagaag ccatctgaag aagaagagca
     4201 ttgtattttt ttccagttgc aaagaggtcc agtatctgta ccgagtgttt tgccggctac
     4261 gtcctggtgt ttctatcctt gcactccatg gtcgacagca gcaaatgaga agaatggaag
     4321 tctataatga gtttgtccgt aagagagctg cagtactctt tgctactgat attgcagcca
     4381 ggggtctgga tttcccggcc gtgaattggg ttcttcagtt tgattgtcct gaggatgcca
     4441 acacatatat tcacagagca ggtagaactg ccaggtacaa agaggatggt gaagctttgc
     4501 taattttgct accctcagaa aaagctatgg tgcagcagct tcttcagaag aaagtacctg
     4561 tgaaggaaat caaaatcaat ccagaaaaac ttatagatgt ccagaaaaaa ttggaatcta
     4621 ttttagctca agatcaagat ttaaaagaaa gagctcaaag gtgtttcgtc tcctatgtac
     4681 gatctgtata tctgatgaag gataaagaag tatttgatgt gagcaagtta cctatacctg
     4741 aatatgccct gtctcttggt cttgctgtgg caccacgcgt aagatttctt cagaaaatgc
     4801 agaaacaacc caccaaagaa ttggtaagga gccaagccga taaagtaatt gagccaaggg
     4861 ctccctccct caccaatgac gaagtggaag aatttagagc ctacttcaat gagaaaatgt
     4921 ccatccttca aaaaggtgga aaaagactcg aagggacaga gcacagacag gataatgata
     4981 ctggtaatga agaacaggaa gaagaagaag acgatgaaga agaaatggaa gagaaactgg
     5041 caaaagcaaa aggatctcaa gccccatctc ttcctaacac cagtgaggca cagaagatca
     5101 aggaagttcc tacacagttc ttggacagag atgaggagga agaagatgct gatttcttga
     5161 aggtgaagcg gcataatgtg tttggattgg accttaaaga cgagaaaaca ttacagaaga
     5221 aagaaccttc taaatccagc atcaagaaaa aaatgaccaa agttgcagaa gcaaaaaaag
     5281 taatgaagag aaattttaaa gtgaataaga agataacatt tactgatgaa ggggagttgg
     5341 ttcagcagtg gccacaaatg cagaaatctg ccatcaagga tgctgaggaa gatgatgaca
     5401 caggtggtat caacttacat aaagcaaagg aaagacttca ggaagaggac aaatttgaca
     5461 aagaagaata taggaaaaaa attaaggcaa agcatcggga gaaaagactg aaagaaaggg
     5521 aagccagaag agaagccaac aagagacaag caaaggccaa agatgaagag gaagcctttc
     5581 tggattggag tgatgatgat gatgatgatg atgatggatt tgatccaagc acactcccag
     5641 atccagataa atacagaagc tctgaagatt cagatagtga agatatggaa aataaaataa
     5701 gtgataccaa gaagaagcag gggatgaaga agaggagcaa cagtgaagtg gaagacgtgg
     5761 gaccaacaag tcataacaga aagaaggcca ggtgggacac tttagagcct ttggataccg
     5821 gcctgtcttt agcagaggat gaagagctgg tgttacatct gctaagaagt caaagctagg
     5881 tcgacctgca gaagcttggc cgccatggcc caacttgttt attgcagctt ataatggtta
     5941 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     6001 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcggga attaattcgg
     6061 cgcagcacca tggcctgaaa taacctctga aagaggaact tggttaggta ccttctgagg
     6121 cggaaagaac cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     6181 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     6241 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     6301 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     6361 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     6421 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctgtt
     6481 aacagcttgg cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     6541 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     6601 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg
     6661 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag caaccatagt
     6721 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     6781 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     6841 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     6901 gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc
     6961 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     7021 gactcttgtt ccaaactgga acaacactca accctatctc gggctattct tttgatttat
     7081 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     7141 acgcgaattt taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct
     7201 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
     7261 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     7321 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
     7381 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     7441 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     7501 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     7561 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     7621 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     7681 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     7741 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     7801 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     7861 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     7921 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     7981 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     8041 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     8101 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     8161 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     8221 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     8281 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     8341 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     8401 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     8461 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     8521 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     8581 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     8641 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     8701 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     8761 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     8821 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     8881 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     8941 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     9001 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     9061 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     9121 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     9181 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     9241 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     9301 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     9361 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     9421 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     9481 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     9541 gaattgtgag cggataacaa tttcacacag gaaacagcta tgacatgatt acgaattaa
//
