LOCUS       pRK5_mCherry-P2A        9650 bp    DNA     circular SYN 13-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9650)
  TITLE     Spatial spread of Ras activity
REFERENCE   2  (bases 1 to 9650)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 13, 2025 from SnapGene 8.0.2
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pRK5_mCherry-P2AT2A-GFP-NUP98-DDX10-KS
FEATURES             Location/Qualifiers
     source          1..9650
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        14..393
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        394..597
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     548..568
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     594..618
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        828..846
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     828..845
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     902..971
                     /label=HN181
     regulatory      933..941
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             939..1646
                     /codon_start=1
                     /label=mCherry (full)
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1258..1278)
                     /label=backbone_R
     CDS             1656..1712
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(1693..1727)
                     /label=HN870
     CDS             1722..1775
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     1752..1796
                     /label=HN871
     primer_bind     complement(1756..1799)
                     /label=HN870
     misc_feature    1756..1775
                     /label=Feature 29
     CDS             join(1776..1778,1779..1781,1782..2492)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     regulatory      1776..1779
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1821..1842)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2082..2101)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2429..2450
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     2446..2529
                     /label=Fragment 1.FOR
     primer_bind     complement(2473..2492)
                     /label=mEGFP_XhoI_re
     CDS             2502..3909
                     /codon_start=1
                     /label=NUP98
                     /translation="MFNKSFGTPFGGGTGGFGTTSTFGQNTGFGTTSGGAFGTSAFGSS
                     NNTGGLFGNSQTKPGGLFGTSSFSQPATSTSTGFGFGTSTGTANTLFGTASTGTSLFSS
                     QNNAFAQNKPTGFGNFGTSTSSGGLFGTTNTTSNPFGSTSGSLFGPSSFTAAPTGTTIK
                     FNPPTGTDTMVKAGVSTNISTKHQCITAMKEYESKSLEELRLEDYQANRKGPQNQVGAG
                     TTTGLFGSSPATSSATGLFSSSTTNSGFAYGQNKTAFGTSTTGFGTNPGGLFGQQNQQT
                     TSLFSKPFGQATTTQNTGFSFGNTSTIGQPSTNTMGLFGVTQASQPGGLFGTATNTSTG
                     TAFGTGTGLFGQTNTGFGAVGSTLFGNNKLTTFGSSTTSAPSFGTTSGGLFGFGTNTSG
                     NSIFGSKPAPGTLGTGLGAGFGTALGAGQASLFGNNQPKIGGPLGTGAFGAPGFNTTTA
                     TLGFGAPQAPV"
     primer_bind     3890..3942
                     /label=Fragment 2.FOR
     primer_bind     complement(3894..3929)
                     /label=Fragment 1.REV
     CDS             3910..5876
                     /codon_start=3
                     /label=DDX10
                     /translation="LDEADRILDMGFADTMNAVIENLPKKRQTLLFSATQTKSVKDLAR
                     LSLKNPEYVWVHEKAKYSTPATLEQNYIVCELQQKISVLYSFLRSHLKKKSIVFFSSCK
                     EVQYLYRVFCRLRPGVSILALHGRQQQMRRMEVYNEFVRKRAAVLFATDIAARGLDFPA
                     VNWVLQFDCPEDANTYIHRAGRTARYKEDGEALLILLPSEKAMVQQLLQKKVPVKEIKI
                     NPEKLIDVQKKLESILAQDQDLKERAQRCFVSYVRSVYLMKDKEVFDVSKLPIPEYALS
                     LGLAVAPRVRFLQKMQKQPTKELVRSQADKVIEPRAPSLTNDEVEEFRAYFNEKMSILQ
                     KGGKRLEGTEHRQDNDTGNEEQEEEEDDEEEMEEKLAKAKGSQAPSLPNTSEAQKIKEV
                     PTQFLDRDEEEEDADFLKVKRHNVFGLDLKDEKTLQKKEPSKSSIKKKMTKVAEAKKVM
                     KRNFKVNKKITFTDEGELVQQWPQMQKSAIKDAEEDDDTGGINLHKAKERLQEEDKFDK
                     EEYRKKIKAKHREKRLKEREARREANKRQAKAKDEEEAFLDWSDDDDDDDDGFDPSTLP
                     DPDKYRSSEDSDSEDMENKISDTKKKQGMKKRSNSEVEDVGPTSHNRKKARWDTLEPLD
                     TGLSLAEDEELVLHLLRSQS"
     CDS             complement(5590..5616)
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     misc_feature    5845..5876
                     /label=Feature 27
     CDS             5877..5927
                     /codon_start=1
                     /label=KS
                     /translation="MMMMNKLVLAQFFFSCL"
     primer_bind     complement(5898..5952)
                     /label=HN933
     primer_bind     complement(5904..5952)
                     /label=HN280
     misc_feature    5928..5930
                     /label=Feature 27
     regulatory      5950..5959
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    5963..6097
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6000..6019)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6054..6073
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        6166..6523
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     complement(6189..6209)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6374..6509
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6436..6455
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(6459..6470)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(6543..6560)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6543..6559)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6552..6574)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6772..7227
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6859..6878)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7069..7090
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7244..7263)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7363..7385
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7423..7441)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7509..7613
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7614..7682,7683..8474)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7832..7851)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8645..9233
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9134..9153
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9387..9404
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        join(9557..9562,9563..9580,9581..9587)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9595..9611
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9600..9622
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9619..9635
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9619..9635
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ttcgagctcg cccgacattg attattgact agttattaat agtaatcaat tacggggtca
       61 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      121 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      181 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      241 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      301 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      361 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
      421 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
      481 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
      541 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt
      601 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
      661 caccgggacc gatccagcct ccgcggccgg gaacggtgca ttggaacgcg gattccccgt
      721 gccaagagtg acgtaagtac cgcctataga gtctataggc ccaccccctt ggcttcgtta
      781 gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt taggtgacac
      841 tatagaataa catccacttt gcctttctct ccacaggtgt ccactcccag gtccaactgc
      901 acctcggttc tatcgattga attcgtaccg gtgccaccat ggtttccaaa ggagaggagg
      961 ataatatggc tataattaaa gagtttatgc ggttcaaggt gcatatggag ggatctgtga
     1021 acggtcacga gttcgaaatc gagggcgaag gcgaggggcg accctatgaa gggacacaga
     1081 cggccaaact gaaggtgacc aagggtggcc ccctgccttt cgcctgggac atcctgtcac
     1141 cccagtttat gtacggctct aaggcttacg ttaagcaccc tgccgatatt ccggactact
     1201 tgaaactgag cttccctgaa gggttcaagt gggaacgggt aatgaatttt gaggatggcg
     1261 gtgtagtcac agttacccag gacagctctc ttcaggacgg agaatttatc tataaggtta
     1321 aacttcgggg cactaacttc ccatcagacg gccccgtgat gcagaagaaa actatgggct
     1381 gggaagccag ctcagaacgc atgtatcccg aggacggagc actgaaggga gaaatcaagc
     1441 agcgactcaa gctgaaggat gggggacatt atgatgctga ggtcaaaacc acctataagg
     1501 ccaagaaacc cgttcagctt cctggggcat acaacgtgaa tatcaaactg gatattacat
     1561 cccataacga agactatacc atcgtggaac agtacgagcg ggccgagggc agacatagca
     1621 cagggggcat ggatgaattg tacaagggaa gcggagctac taacttcagc ctgctgaagc
     1681 aggctggaga cgtggaggag aaccctggac ctggaagcgg agagggcaga ggaagtcttc
     1741 taacatgcgg tgacgtggag gagaatcccg gcccaatggt gagcaagggc gaggagctgt
     1801 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
     1861 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
     1921 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg
     1981 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     2041 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
     2101 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
     2161 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
     2221 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc
     2281 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
     2341 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccaagctga
     2401 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     2461 ggatcactct cggcatggac gagctgtaca agaccggcgg catgtttaac aaatcatttg
     2521 gaacaccctt tgggggtggc acaggtggct ttggcacaac ttcaacattt ggacagaata
     2581 ctggctttgg cactactagt ggaggggcat ttggaacatc tgcatttggt tctagcaaca
     2641 atactggagg cctctttgga aattcacaga ctaaaccagg aggattgttt ggaaccagtt
     2701 catttagcca gccagctacc tccacaagca ctggctttgg gtttggtacg tcaacaggaa
     2761 cagcaaatac cttgtttgga actgcaagca cagggaccag tctcttctca tcccaaaaca
     2821 atgcctttgc acaaaataaa ccaactggct ttggcaattt tggaaccagt actagcagtg
     2881 gaggactctt tggaaccaca aataccacct ctaatccttt tggcagcaca tctggctccc
     2941 tctttgggcc aagtagtttt acagctgctc ctactgggac tactattaaa tttaaccctc
     3001 caactggtac agatactatg gtcaaagctg gagttagcac taacataagt accaagcacc
     3061 agtgtattac tgctatgaaa gaatatgaaa gcaagtcact agaggaactt cgtttagagg
     3121 attatcaggc taacaggaag ggcccacaga accaggtggg agcaggtacc acaactggct
     3181 tgtttgggtc ttctccagcc acttccagcg caacaggact cttcagctcc tccaccacta
     3241 attcaggctt tgcatatggt cagaacaaaa ctgcctttgg aactagtaca actggatttg
     3301 gaacaaatcc aggtggtctc tttggccaac agaatcagca gactaccagc ctcttcagca
     3361 aaccatttgg ccaggctaca accacccaga acactggctt ttcctttggt aataccagca
     3421 ccataggaca gccaagcacc aacaccatgg gattatttgg agtaacccaa gcctcacagc
     3481 ctggaggtct ttttgggaca gctacaaaca ccagcactgg gacagcattt ggaacaggaa
     3541 caggtctctt tgggcagacc aatactggat ttggtgctgt tggttcgacc ctgtttggca
     3601 ataacaagct tactacattt ggaagcagca caaccagtgc accttcattt ggtacaacca
     3661 gtggcgggct ctttggtttt ggcacaaata ccagtgggaa tagtattttt ggaagtaaac
     3721 cagcacctgg gactcttgga actgggcttg gtgcaggatt tggaacagct cttggtgctg
     3781 gacaggcatc tttgtttggg aacaaccaac ctaagattgg agggcctctt ggtacaggag
     3841 cctttggggc ccctggattt aatactacga cagccacttt gggctttgga gccccccagg
     3901 ccccagtagt tcttgatgaa gcagatagaa tcttggatat gggctttgct gataccatga
     3961 atgctgttat tgaaaatctc cccaagaaac gtcagacttt acttttctca gcaacacaaa
     4021 ctaaatctgt aaaggacctt gcacgcttga gtttgaaaaa ccctgagtat gtctgggttc
     4081 atgaaaaagc aaaatatagc acccctgcca ctttggaaca gaactacata gtctgtgagc
     4141 tgcagcaaaa aataagtgtg ctgtattcct ttttgagaag ccatctgaag aagaagagca
     4201 ttgtattttt ttccagttgc aaagaggtcc agtatctgta ccgagtgttt tgccggctac
     4261 gtcctggtgt ttctatcctt gcactccatg gtcgacagca gcaaatgaga agaatggaag
     4321 tctataatga gtttgtccgt aagagagctg cagtactctt tgctactgat attgcagcca
     4381 ggggtctgga tttcccggcc gtgaattggg ttcttcagtt tgattgtcct gaggatgcca
     4441 acacatatat tcacagagca ggtagaactg ccaggtacaa agaggatggt gaagctttgc
     4501 taattttgct accctcagaa aaagctatgg tgcagcagct tcttcagaag aaagtacctg
     4561 tgaaggaaat caaaatcaat ccagaaaaac ttatagatgt ccagaaaaaa ttggaatcta
     4621 ttttagctca agatcaagat ttaaaagaaa gagctcaaag gtgtttcgtc tcctatgtac
     4681 gatctgtata tctgatgaag gataaagaag tatttgatgt gagcaagtta cctatacctg
     4741 aatatgccct gtctcttggt cttgctgtgg caccacgcgt aagatttctt cagaaaatgc
     4801 agaaacaacc caccaaagaa ttggtaagga gccaagccga taaagtaatt gagccaaggg
     4861 ctccctccct caccaatgac gaagtggaag aatttagagc ctacttcaat gagaaaatgt
     4921 ccatccttca aaaaggtgga aaaagactcg aagggacaga gcacagacag gataatgata
     4981 ctggtaatga agaacaggaa gaagaagaag acgatgaaga agaaatggaa gagaaactgg
     5041 caaaagcaaa aggatctcaa gccccatctc ttcctaacac cagtgaggca cagaagatca
     5101 aggaagttcc tacacagttc ttggacagag atgaggagga agaagatgct gatttcttga
     5161 aggtgaagcg gcataatgtg tttggattgg accttaaaga cgagaaaaca ttacagaaga
     5221 aagaaccttc taaatccagc atcaagaaaa aaatgaccaa agttgcagaa gcaaaaaaag
     5281 taatgaagag aaattttaaa gtgaataaga agataacatt tactgatgaa ggggagttgg
     5341 ttcagcagtg gccacaaatg cagaaatctg ccatcaagga tgctgaggaa gatgatgaca
     5401 caggtggtat caacttacat aaagcaaagg aaagacttca ggaagaggac aaatttgaca
     5461 aagaagaata taggaaaaaa attaaggcaa agcatcggga gaaaagactg aaagaaaggg
     5521 aagccagaag agaagccaac aagagacaag caaaggccaa agatgaagag gaagcctttc
     5581 tggattggag tgatgatgat gatgatgatg atgatggatt tgatccaagc acactcccag
     5641 atccagataa atacagaagc tctgaagatt cagatagtga agatatggaa aataaaataa
     5701 gtgataccaa gaagaagcag gggatgaaga agaggagcaa cagtgaagtg gaagacgtgg
     5761 gaccaacaag tcataacaga aagaaggcca ggtgggacac tttagagcct ttggataccg
     5821 gcctgtcttt agcagaggat gaagagctgg tgttacatct gctaagaagt caaagcatga
     5881 tgatgatgaa taagttggtt ctagcgcagt tcttcttctc ctgtctatag gtcgacctgc
     5941 agaagcttgg ccgccatggc ccaacttgtt tattgcagct tataatggtt acaaataaag
     6001 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     6061 gtccaaactc atcaatgtat cttatcatgt ctggatcggg aattaattcg gcgcagcacc
     6121 atggcctgaa ataacctctg aaagaggaac ttggttaggt accttctgag gcggaaagaa
     6181 ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     6241 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     6301 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     6361 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     6421 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     6481 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctgt taacagcttg
     6541 gcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     6601 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     6661 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc
     6721 cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct
     6781 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     6841 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     6901 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     6961 ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct
     7021 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     7081 tccaaactgg aacaacactc aaccctatct cgggctattc ttttgattta taagggattt
     7141 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     7201 ttaacaaaat attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg
     7261 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     7321 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     7381 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     7441 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     7501 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     7561 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     7621 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     7681 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     7741 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     7801 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     7861 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     7921 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     7981 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     8041 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     8101 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     8161 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     8221 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     8281 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     8341 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     8401 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     8461 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     8521 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     8581 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     8641 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     8701 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     8761 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     8821 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     8881 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     8941 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     9001 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     9061 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     9121 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     9181 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     9241 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     9301 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     9361 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     9421 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     9481 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     9541 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     9601 gcggataaca atttcacaca ggaaacagct atgacatgat tacgaattaa
//
