LOCUS       Exported                8687 bp ds-DNA     circular SYN 26-MAR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sunday, Apr 13, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Imported using the Genbank importer. File name: 
            188-MSCV-eGFP-IRES-NUP98-JARID1A.gb Serial Cloner Genbank Format 
            SerialCloner_Type=DNA SerialCloner_Comments=Fragment  (BamHI/BsiWI) 
            from 164-MSCV-rtTA3-IRES-NUP98-JARID 1A.xdna 
            SerialCloner_Ends=35,4,gatc,4,gtac
FEATURES             Location/Qualifiers
     source          1..8687
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    8..579
                     /label=IRES
     misc_feature    604..2142
                     /label=NUP98
     misc_feature    2143..2757
                     /label=KMD5A
     misc_feature    2758..2808
                     /label=KS
     misc_feature    2809..2811
                     /label=stop
     misc_feature    2908..3422
                     /label=3`LTR
     misc_binding    complement(3582..3604)
                     /label=LacO
     promoter        complement(3609..3638)
                     /label=lac
     rep_origin      complement(3944..4572)
                     /direction=LEFT
                     /label=ColE1 origin
     CDS             complement(4724..5383)
                     /label=AmpR
     promoter        complement(5623..5651)
                     /label=Amp prom
     CDS             complement(6020..6088)
                     /label=LacZ alpha
     misc_feature    7094..7918
                     /label=MSCV promoter
     misc_feature    7848..7870
                     /label=MSCV fwd
     CDS             7948..8661
                     /label=EGFP
ORIGIN
        1 gcaattccgc ccctctccct cccccccccc ctaacgttac tggccgaagc cgcttggaat
       61 aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg
      121 tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc
      181 tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt
      241 cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg
      301 acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac
      361 cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg
      421 tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg
      481 ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc
      541 cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataagcttac cggtgccacc
      601 atgtttaaca aatcatttgg aacacccttt gggggtggca caggtggctt tggcacaact
      661 tcaacatttg gacagaatac tggctttggc actactagtg gaggggcatt tggaacatct
      721 gcatttggtt ctagcaacaa tactggaggc ctctttggaa attcacagac taaaccagga
      781 ggattgtttg gaaccagttc atttagccag ccagctacct ccacaagcac tggctttggg
      841 tttggtacgt caacaggaac agcaaatacc ttgtttggaa ctgcaagcac agggaccagt
      901 ctcttctcat cccaaaacaa tgcctttgca caaaataaac caactggctt tggcaatttt
      961 ggaaccagta ctagcagtgg aggactcttt ggaaccacaa ataccacctc taatcctttt
     1021 ggcagcacat ctggctccct ctttgggcca agtagtttta cagctgctcc tactgggact
     1081 actattaaat ttaaccctcc aactggtaca gatactatgg tcaaagctgg agttagcact
     1141 aacataagta ccaagcacca gtgtattact gctatgaaag aatatgaaag caagtcacta
     1201 gaggaacttc gtttagagga ttatcaggct aacaggaagg gcccacagaa ccaggtggga
     1261 gcaggtacca caactggctt gtttgggtct tctccagcca cttccagcgc aacaggactc
     1321 ttcagctcct ccaccactaa ttcaggcttt gcatatggtc agaacaaaac tgcctttgga
     1381 actagtacaa ctggatttgg aacaaatcca ggtggtctct ttggccaaca gaatcagcag
     1441 actaccagcc tcttcagcaa accatttggc caggctacaa ccacccagaa cactggcttt
     1501 tcctttggta ataccagcac cataggacag ccaagcacca acaccatggg attatttgga
     1561 gtaacccaag cctcacagcc tggaggtctt tttgggacag ctacaaacac cagcactggg
     1621 acagcatttg gaacaggaac aggtctcttt gggcagacca atactggatt tggtgctgtt
     1681 ggttcgaccc tgtttggcaa taacaagctt actacatttg gaagcagcac aaccagtgca
     1741 ccttcatttg gtacaaccag tggcgggctc tttggttttg gcacaaatac cagtgggaat
     1801 agtatttttg gaagtaaacc agcacctggg actcttggaa ctgggcttgg tgcaggattt
     1861 ggaacagctc ttggtgctgg acaggcatct ttgtttggga acaaccaacc taagattgga
     1921 gggcctcttg gtacaggagc ctttggggcc cctggattta atactacgac agccactttg
     1981 ggctttggag ccccccaggc cccagtagct ttgacagatc caaatgcttc tgctgcccag
     2041 caggctgttc tccagcagca catcaatagt ctaacatact caccttttgg agactctcct
     2101 ctcttccgga atccgatgtc agaccctaag aagaaggaag aggatgacag catggaagag
     2161 aaaccactaa aagtgaaagg aaaggactct tcagagaaga aacggaaacg gaagctagaa
     2221 aaggtagagc aactttttgg agaaggaaaa cagaagtcca aggagttaaa gaaaatggac
     2281 aaacctagaa agaagaaatt aaaattaggt gcagacaaat caaaggagct gaataaactg
     2341 gccaagaaac tagcaaaaga agaagagaga aagaaaaaga aggagaaggc tgctgcagcc
     2401 aaagttgaac ttgtgaaaga gagcactgaa aagaaaagag agaaaaaggt gctggacatc
     2461 ccctcaaagt atgactggtc aggagcagag gagtctgatg atgagaatgc tgtgtgcgca
     2521 gcacagaact gccaaaggcc ctgcaaggac aaggtagact gggtacaatg tgatggtggc
     2581 tgtgatgagt ggtttcatca agtttgtgtg ggtgtatctc cagaaatggc tgaaaatgaa
     2641 gattacatct gtataaactg tgcaaagaag caggggccag ttagcccagg tccagcacca
     2701 cctccttcct tcataatgag ctacaaacta ccaatggagg atcttaaaga gaccagtatg
     2761 atgatgatga ataagttggt tctagcgcag ttcttcttct cctgtctatg aaattcgcca
     2821 gcacagtgct cgagcacgga catcgacctg cagccaagct tatcgataaa ataaaagatt
     2881 ttatttagtc tccagaaaaa ggggggaatg aaagacccca cctgtaggtt tggcaagcta
     2941 gcttaagtaa cgccattttg caaggcatgg aaaatacata actgagaata gagaagttca
     3001 gatcaaggtt aggaacagag agacagcaga atatgggcca aacaggatat ctgtggtaag
     3061 cagttcctgc cccggctcag ggccaagaac agatggtccc cagatgcggt cccgccctca
     3121 gcagtttcta gagaaccatc agatgtttcc agggtgcccc aaggacctga aatgaccctg
     3181 tgccttattt gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc
     3241 ccgagctcaa taaaagagcc cacaacccct cactcggcgc gccagtcctc cgatagactg
     3301 cgtcgcccgg gtacccgtgt atccaataaa ccctcttgca gttgcatccg acttgtggtc
     3361 tcgctgttcc ttgggagggt ctcctctgag tgattgacta cccgtcagcg ggggtctttc
     3421 atgggtaaca gtttcttgaa gttggagaac aacattctga gggtaggagt cgaatattaa
     3481 gtaatcctga ctcaattagc cactgttttg aatccacata ctccaatact cctgaaatag
     3541 ttcattatgg acagcgcaga aagagctggg gagaattgtg aaattgttat ccgctcacaa
     3601 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     3661 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     3721 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     3781 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     3841 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     3901 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     3961 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     4021 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     4081 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     4141 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     4201 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     4261 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     4321 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     4381 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     4441 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     4501 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     4561 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     4621 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     4681 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     4741 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     4801 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     4861 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     4921 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     4981 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     5041 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     5101 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     5161 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     5221 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     5281 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     5341 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     5401 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     5461 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     5521 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     5581 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     5641 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     5701 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     5761 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
     5821 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
     5881 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag
     5941 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga
     6001 gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat
     6061 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
     6121 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gcgcaaggaa
     6181 tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc
     6241 cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat
     6301 gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg
     6361 tccggcgtag aggcgattag tccaatttgt taaagacagg atatcagtgg tccaggctct
     6421 agttttgact caacaatatc accagctgaa gcctatagag tacgagccat agataaaata
     6481 aaagatttta tttagtctcc agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg
     6541 caagctagct taagtaacgc cattttgcaa ggcatggaaa atacataact gagaatagag
     6601 aagttcagat caaggttagg aacagagaga cagcagaata tgggccaaac aggatatctg
     6661 tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggtccccag atgcggtccc
     6721 gccctcagca gtttctagag aaccatcaga tgtttccagg gtgccccaag gacctgaaat
     6781 gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt
     6841 ctgctccccg agctcaataa aagagcccac aacccctcac tcggcgcgcc agtcctccga
     6901 tagactgcgt cgcccgggta cccgtattcc caataaagcc tcttgctgtt tgcatccgaa
     6961 tcgtggactc gctgatcctt gggagggtct cctcagattg attgactgcc cacctcgggg
     7021 gtctttcatt tggaggttcc accgagattt ggagacccct gcccagggac caccgacccc
     7081 cccgccggga ggtaagctgg ccagcggtcg tttcgtgtct gtctctgtct ttgtgcgtgt
     7141 ttgtgccggc atctaatgtt tgcgcctgcg tctgtactag ttagctaact agctctgtat
     7201 ctggcggacc cgtggtggaa ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg
     7261 tcccagggac tttgggggcc gtttttgtgg cccgacctga ggaagggagt cgatgtggaa
     7321 tccgaccccg tcaggatatg tggttctggt aggagacgag aacctaaaac agttcccgcc
     7381 tccgtctgaa tttttgcttt cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagcg
     7441 ctgcagcatc gttctgtgtt gtctctgtct gactgtgttt ctgtatttgt ctgaaaatta
     7501 gggccagact gttaccactc ccttaagttt gaccttaggt cactggaaag atgtcgagcg
     7561 gatcgctcac aaccagtcgg tagatgtcaa gaagagacgt tgggttacct tctgctctgc
     7621 agaatggcca acctttaacg tcggatggcc gcgagacggc acctttaacc gagacctcat
     7681 cacccaggtt aagatcaagg tcttttcacc tggcccgcat ggacacccag accaggtccc
     7741 ctacatcgtg acctgggaag ccttggcttt tgacccccct ccctgggtca agccctttgt
     7801 acaccctaag cctccgcctc ctcttcctcc atccgccccg tctctccccc ttgaacctcc
     7861 tcgttcgacc ccgcctcgat cctcccttta tccagccctc actccttctc taggcgccgg
     7921 aattgaagat caacgtacgc caccatggtg agcaagggcg aggagctgtt caccggggtg
     7981 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtctggc
     8041 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     8101 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
     8161 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     8221 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     8281 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     8341 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     8401 atcatggccg acaagcagaa gaacggcatc aaggcgaact tcaagatccg ccacaacatc
     8461 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     8521 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
     8581 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     8641 ggcatggacg agctgtacaa gtaatgagga tccgcggccg cactaga
//
