LOCUS       YY100_pAAV-CAG(short)-A 6727 bp ds-DNA     circular     23-FEB-2024
DEFINITION  .
COMMENT     Sequence created by bulk assembly. Backbone 5' end ligation method:
            concatenation starting/ending at bases [gttaacGTGAGCAAGGGCGAGGAGC]
            3' end ligation method: concatenation starting/ending at bases
            [ggatccggcgcgcc] Insert 5' end ligation method: concatenation from
            start/end of sequence 3' end ligation method: concatenation from
            start/end of sequence Backbone 5' end ligation method:
            concatenation starting/ending at bases [gttaacGTGAGCAAGGGCGAGGAGC]
            3' end ligation method: concatenation starting/ending at bases
            [ggatccggcgcgcc]
FEATURES             Location/Qualifiers
     misc_feature    1..141
                     /label="L-ITR"
                     /ApEinfo_revcolor="#3fbf3f"
                     /ApEinfo_fwdcolor="#3fbf3f"
     misc_feature    151..1277
                     /label="KC103F"
                     /ApEinfo_revcolor="#3f3fbf"
                     /ApEinfo_fwdcolor="#3f3fbf"
     promoter        164..1263
                     /label="CAG"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          1270..1310
                     /label="5-AnsACR to pAAV-CAG"
                     /note="sequence: aacggtaccgcgggcccgggatccATGGCAACCCTGCCCGC"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    1288..1293
                     /label="BamHI"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1294..2088
                     /label="AnsACR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(2073..2112)
                     /label="3-AnsACR to Hpa1 to EYFP"
                     /note="sequence: CTCCTCGCCCTTGCTCACgttaacGGCGCTGCCTGTCCGC"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2089..2094
                     /label="HpaI"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     exon            2095..2808
                     /label="EYFP (no start)"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(2338..2357)
                     /label="EYFP R266 Xue 12"
                     /note="sequence: ATGGCGGACTTGAAGAAGTC"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    2812..2817
                     /label="NheI"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2818..2818
                     /label="EcoRI"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2819..2823
                     /label="EcoRI"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2824..2829
                     /label="HindIII"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2844..3452
                     /label="WPRE"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     polyA_signal    3472..3950
                     /label="hGH polyA"
                     /ApEinfo_revcolor="#8000ff"
                     /ApEinfo_fwdcolor="#8000ff"
     misc_feature    3990..4130
                     /label="R-ITR"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     rep_origin      4222..4528
                     /label="f1 Origin"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    5047..5904
                     /label="AmpR"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     rep_origin      6055..6722
                     /label="pUC ori"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtaacac tagttattaa tagtaatcaa
      181 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
      241 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
      301 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
      361 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
      421 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
      481 ctacttggca gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca
      541 cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg tatttattta
      601 ttttttaatt attttgtgca gcgatggggg cggggggggg gggggggcgc gcgccaggcg
      661 gggcggggcg gggcgagggg cggggcgggg cgaggcggag aggtgcggcg gcagccaatc
      721 agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata
      781 aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgcgctg ccttcgcccc gtgccccgct
      841 ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac cgcgttactc ccacaggtga
      901 gcgggcggga cggcccttct cctccgggct gtaattagcg cttggtttaa tgacggcttg
      961 tttcttttct gtggctgcgt gaaagccttg aggggctccg ggagcgccgg caggaaggaa
     1021 atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct
     1081 cggggctgtc cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc
     1141 ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt
     1201 ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa
     1261 ttgcggccca acggtaccgc gggcccggga tccATGGCAA CCCTGCCCGC CATCGACATG
     1321 GTCTACGTGG TCCAAGGCAT CGTGTCCGTG GTGGCCAGCA CACACGCCAT TTTCCTGCTG
     1381 TTTAGATACT TCAAAAGCAG AGGACCTTGG CAGGAGGCCT TCGTGGTGTG CGTGCAGAGC
     1441 TTCTGCGCCA TCAGCAGCCT GGTGCTTACA GCCGTGGACC AGAGCATCGA ATCCGGCATG
     1501 GATGTGCTGA ACGGCGCCGG CGACACCATC AACATCCTGA GATTCCTGGG ATGGTTCGTG
     1561 ACCTGTCCTG TGCAGGTGGC CGCCCTGTGC AATCTGAATC ACATCGCCGA TTACGCCAGC
     1621 GCCGAGGGCA GCACCCACCA GGGAGGCGCT GGCACCGTGA ACGAGGCCAC CATGATGGCC
     1681 ATCATGACCA ACATGCTGGT CTGCGCCACC ATGATGGCTA CCGCTGCTAG CCCTACATTC
     1741 AAGGTGATCT ACTTTCTGGT GGGCGGTGTT CTCTGTGCCT ACCTGTTCTA TGTGTTCGGC
     1801 TCTGTGTTCC GGAAGTACCA GAGACTGGTT CCCGAGTCTA AGAGCGCTAA AGTGCTGCTG
     1861 CCTGTGTTTT ACCTGGCCTG GTCCCTGTTC CCCATCACCG CCCTGCTGTC TCCATACGGC
     1921 TTCAGCACCG TGTCTGAATC TACAGCCGGA GCCATGTTCT CCCTGGCCGA CCTGCCTGCC
     1981 AAGAACCTGA TCGTGGTGCT CATGGGCAAC GTGCGGAGAA GCATCGAAAG CAAGACCAGC
     2041 GGCACAGCTA CAGGCAGCCT GAGCTCTCTG CAGCGGACAG GCAGCGCCgt taacGTGAGC
     2101 AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA
     2161 AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG
     2221 ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC
     2281 ACCTTCGGCT ACGGCCTGCA GTGCTTCGCC CGCTACCCCG ACCACATGAA GCAGCACGAC
     2341 TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC
     2401 GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC
     2461 ATCGAGCTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG
     2521 TACAACTACA ACAGCCACAA CGTCTATATC ATGGCCGACA AGCAGAAGAA CGGCATCAAG
     2581 GTGAACTTCA AGATCCGCCA CAACATCGAG GACGGCAGCG TGCAGCTCGC CGACCACTAC
     2641 CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC
     2701 TACCAGTCCG CCCTGAGCAA AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAG
     2761 TTCGTGACCG CCGCCGGGAT CACTCTCGGC ATGGACGAGC TGTACAAGtg agctagcgaa
     2821 ttcaagctta aattcgatat caagcttatc gataatcaac ctctggatta caaaatttgt
     2881 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     2941 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     3001 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     3061 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     3121 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     3181 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     3241 tcggggaaat catcgtcctt tccttggctg ctcgcctatg ttgccacctg gattctgcgc
     3301 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     3361 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     3421 tccctttggg ccgcctcccc gcatcgatac cgagcgctgc tcgagagatc tacgggtggc
     3481 atccctgtga cccctcccca gtgcctctcc tggccctgga agttgccact ccagtgccca
     3541 ccagccttgt cctaataaaa ttaagttgca tcattttgtc tgactaggtg tccttctata
     3601 atattatggg gtggaggggg gtggtatgga gcaaggggca agttgggaag acaacctgta
     3661 gggcctgcgg ggtctattgg gaaccaagct ggagtgcagt ggcacaatct tggctcactg
     3721 caatctccgc ctcctgggtt caagcgattc tcctgcctca gcctcccgag ttgttgggat
     3781 tccaggcatg catgaccagg ctcagctaat ttttgttttt ttggtagaga cggggtttca
     3841 ccatattggc caggctggtc tccaactcct aatctcaggt gatctaccca ccttggcctc
     3901 ccaaattgct gggattacag gcgtgaacca ctgctccctt ccctgtcctt ctgattttgt
     3961 aggtaaccac gtgcggaccg agcggccgca ggaaccccta gtgatggagt tggccactcc
     4021 ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg
     4081 ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat
     4141 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca
     4201 tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     4261 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     4321 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     4381 tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt
     4441 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     4501 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcgggcta ttcttttgat
     4561 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     4621 tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca
     4681 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
     4741 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     4801 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
     4861 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     4921 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     4981 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     5041 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     5101 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     5161 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     5221 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     5281 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     5341 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     5401 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     5461 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     5521 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     5581 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     5641 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     5701 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     5761 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     5821 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     5881 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     5941 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     6001 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     6061 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     6121 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     6181 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     6241 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     6301 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     6361 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     6421 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     6481 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     6541 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     6601 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     6661 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     6721 cacatgt
//