LOCUS       YY101_pAAV-CAG(short)-F 6736 bp ds-DNA     circular     23-FEB-2024
DEFINITION  .
COMMENT     Sequence created by bulk assembly. Backbone 5' end ligation method:
            concatenation starting/ending at bases [gttaacGTGAGCAAGGGCGAGGAGC]
            3' end ligation method: concatenation starting/ending at bases
            [ggatccggcgcgcc] Insert 5' end ligation method: concatenation from
            start/end of sequence 3' end ligation method: concatenation from
            start/end of sequence Backbone 5' end ligation method:
            concatenation starting/ending at bases [gttaacGTGAGCAAGGGCGAGGAGC]
            3' end ligation method: concatenation starting/ending at bases
            [ggatccggcgcgcc]
FEATURES             Location/Qualifiers
     misc_feature    1..141
                     /label="L-ITR"
                     /ApEinfo_revcolor="#3fbf3f"
                     /ApEinfo_fwdcolor="#3fbf3f"
     misc_feature    151..1277
                     /label="KC103F"
                     /ApEinfo_revcolor="#3f3fbf"
                     /ApEinfo_fwdcolor="#3f3fbf"
     promoter        164..1263
                     /label="CAG"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          1135..1153
                     /label="YY41 CAGF"
                     /note="sequence: ttcggcttctggcgtgtga"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          1270..1315
                     /label="5-FtACR to pAAV-CAG"
                     /note="sequence: aacggtaccgcgggcccgggatccATGGTGTCCGGCGAGGATAATG"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1288..1293
                     /label="BamHI"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1294..2097
                     /label="FtACR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(2077..2121)
                     /label="3-FtACR to Hpa1 to EYFP"
                     /note="sequence: CTCCTCGCCCTTGCTCACgttaacGCTGTGGCTGCTTGTCTGGAT"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    2098..2103
                     /label="HpaI"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     exon            2104..2817
                     /label="EYFP (no start)"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(2347..2366)
                     /label="EYFP R266 Xue 12"
                     /note="sequence: ATGGCGGACTTGAAGAAGTC"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    2821..2826
                     /label="NheI"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2827..2827
                     /label="EcoRI"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2828..2832
                     /label="EcoRI"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2833..2838
                     /label="HindIII"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2853..3461
                     /label="WPRE"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     polyA_signal    3481..3959
                     /label="hGH polyA"
                     /ApEinfo_revcolor="#8000ff"
                     /ApEinfo_fwdcolor="#8000ff"
     misc_feature    3999..4139
                     /label="R-ITR"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     rep_origin      4231..4537
                     /label="f1 Origin"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    5056..5913
                     /label="AmpR"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     rep_origin      6064..6731
                     /label="pUC ori"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtaacac tagttattaa tagtaatcaa
      181 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
      241 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
      301 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
      361 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
      421 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
      481 ctacttggca gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca
      541 cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg tatttattta
      601 ttttttaatt attttgtgca gcgatggggg cggggggggg gggggggcgc gcgccaggcg
      661 gggcggggcg gggcgagggg cggggcgggg cgaggcggag aggtgcggcg gcagccaatc
      721 agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata
      781 aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgcgctg ccttcgcccc gtgccccgct
      841 ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac cgcgttactc ccacaggtga
      901 gcgggcggga cggcccttct cctccgggct gtaattagcg cttggtttaa tgacggcttg
      961 tttcttttct gtggctgcgt gaaagccttg aggggctccg ggagcgccgg caggaaggaa
     1021 atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct
     1081 cggggctgtc cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc
     1141 ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt
     1201 ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa
     1261 ttgcggccca acggtaccgc gggcccggga tccATGGTGT CCGGCGAGGA TAATGTGTCT
     1321 ATCGCCTACG CCATGATGGT CCTGGTGTCT GGCATCAGCG TGGTCCAGGC CATCTGGTAC
     1381 ACCATCCAAA CAGCCAGAGG CAAGAACCAC AGCTGGCGGC CAGCTCTGGT CATCGGCGCT
     1441 GAGGCCTTTC AGTACATGGT GCGGCTGGCC GTGTCCCTGA CCGACAGCAA CATCTCCAGC
     1501 GGACTGACCG TGATGAACAG CAGAGGCCTG GAAGTGGATA TCGGCACCTA CCTGGGCTGG
     1561 TTCTTCACCT GTCCTCTGCT GGTGGACGCC ATTGGCAATG TGGAGGACGA CATCATCTAC
     1621 AGCGTTGAGC TGTACCGGGC CGCCATGACC AACACCGAGG CCGTGTACAG CCCTAGAAGA
     1681 GTGTGGTGGA TGAGCGCCGT GATGTCTGGC ATGCTGTGTA CAGTGGTGGT GGCCTCTCTG
     1741 GCTGAAAAGA CCACCCAGAT CGCCGCTGTC TTTTGCGTGG GCGGAGCCCT GTGCATGGTG
     1801 CTCTTCGCCG GCATCGGAAG AGCCATTAGA CGGTGCCTCG ACCACGGCGC TGATAGACTG
     1861 ACAGCATATA GCCTGCTGAT CGTGTTCTAC ACAGGCTGGA GCCTGTTCCC CCTGATCTGG
     1921 GTGCTTTCTC CTCTGGGATA CGACGTGCTG GATGCCGTGA CCGCCAGGGC CCTGTTCGGC
     1981 TTCGCCGACC TGATCGCCAA AAACCTGCTG AGCGTGCTGC TGACAAGACT GACCGTGATG
     2041 AGCGTGAACG CCAAGCAGGC TGTGCTGGAA CTGAAGATCC AGACAAGCAG CCACAGCgtt
     2101 aacGTGAGCA AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC
     2161 GGCGACGTAA ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC
     2221 GGCAAGCTGA CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC
     2281 CTCGTGACCA CCTTCGGCTA CGGCCTGCAG TGCTTCGCCC GCTACCCCGA CCACATGAAG
     2341 CAGCACGACT TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC
     2401 TTCAAGGACG ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG
     2461 GTGAACCGCA TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC
     2521 AAGCTGGAGT ACAACTACAA CAGCCACAAC GTCTATATCA TGGCCGACAA GCAGAAGAAC
     2581 GGCATCAAGG TGAACTTCAA GATCCGCCAC AACATCGAGG ACGGCAGCGT GCAGCTCGCC
     2641 GACCACTACC AGCAGAACAC CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC
     2701 TACCTGAGCT ACCAGTCCGC CCTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC
     2761 CTGCTGGAGT TCGTGACCGC CGCCGGGATC ACTCTCGGCA TGGACGAGCT GTACAAGtga
     2821 gctagcgaat tcaagcttaa attcgatatc aagcttatcg ataatcaacc tctggattac
     2881 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     2941 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     3001 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     3061 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     3121 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     3181 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     3241 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctatgt tgccacctgg
     3301 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     3361 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     3421 agtcggatct ccctttgggc cgcctccccg catcgatacc gagcgctgct cgagagatct
     3481 acgggtggca tccctgtgac ccctccccag tgcctctcct ggccctggaa gttgccactc
     3541 cagtgcccac cagccttgtc ctaataaaat taagttgcat cattttgtct gactaggtgt
     3601 ccttctataa tattatgggg tggagggggg tggtatggag caaggggcaa gttgggaaga
     3661 caacctgtag ggcctgcggg gtctattggg aaccaagctg gagtgcagtg gcacaatctt
     3721 ggctcactgc aatctccgcc tcctgggttc aagcgattct cctgcctcag cctcccgagt
     3781 tgttgggatt ccaggcatgc atgaccaggc tcagctaatt tttgtttttt tggtagagac
     3841 ggggtttcac catattggcc aggctggtct ccaactccta atctcaggtg atctacccac
     3901 cttggcctcc caaattgctg ggattacagg cgtgaaccac tgctcccttc cctgtccttc
     3961 tgattttgta ggtaaccacg tgcggaccga gcggccgcag gaacccctag tgatggagtt
     4021 ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg
     4081 acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg
     4141 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca
     4201 aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     4261 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     4321 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     4381 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt
     4441 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     4501 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcgggctat
     4561 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     4621 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact
     4681 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
     4741 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     4801 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga
     4861 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     4921 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     4981 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     5041 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     5101 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     5161 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     5221 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     5281 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     5341 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     5401 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     5461 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     5521 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     5581 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     5641 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     5701 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     5761 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     5821 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     5881 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     5941 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     6001 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     6061 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     6121 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     6181 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     6241 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     6301 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     6361 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     6421 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     6481 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     6541 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     6601 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     6661 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     6721 ccttttgctc acatgt
//