LOCUS       Exported                2203 bp ds-DNA     circular SYN 31-MAR-2024
DEFINITION  Generated by Gibson/Homology Cloning (DVLA backbone w terminator 
            overhangs, EML001 with UNS - lambda t0 terminator).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Apr 11, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..1148
                     /label=Extracted region from T2m
     CDS             complement(8..868)
                     /label=bla
     primer_bind     1040..1060
                     /label=SC189/Part.Seq.FOR
     misc_feature    1131..1261
                     /label=EML001 with UNS - lambda t0 terminator (concate...
     misc_feature    1131..1148
                     /label=Ligation
     misc_feature    1149..1261
                     /label=Extracted region from EML001 with UNS
     terminator      complement(1149..1243)
                     /label=lambda t0 terminator
     primer_bind     1149..1170
                     /label=EML001 with UNS - lambda t0 terminator (1 F) - ...
     primer_bind     complement(1220..1243)
                     /label=EML001 with UNS - lambda t0 terminator (95 R) -...
     misc_feature    1244..3351
                     /label=DVLA backbone w terminator overhangs (concatena...
     misc_feature    1244..1261
                     /label=Ligation
     misc_feature    1248..3347
                     /label=DVLA
     misc_feature    1256..3333
                     /label=DVLA
     misc_feature    1262..2203
                     /label=Extracted region from T2m
     primer_bind     complement(1331..1351)
                     /label=SC190/Part.Seq.REV
ORIGIN
        1 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
       61 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
      121 ctggccccag tgctgcaatg ataccgcgcg acccacgctc accggctcca gatttatcag
      181 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
      241 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
      301 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
      361 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
      421 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
      481 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
      541 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
      601 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
      661 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
      721 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
      781 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
      841 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
      901 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
      961 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     1021 tcatgacatt aacctataaa aataggcgta tcacgaggca gaatttcaga taaaaaaaat
     1081 ccttagcttt cgctaaggat gatttctgga attcgcggcc gcttctagag actagtgggt
     1141 ctcaaggtga ctcctgttga tagatccagt aatgacctca gaactccatc tggatttgtt
     1201 cagaacgctc ggttgccgcc gggcgttttt tattggtgag aatgcttaga gacctactag
     1261 tagcggccgc tgcaggcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     1321 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     1381 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     1441 ttgctggcgt ttttccacag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     1501 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     1561 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     1621 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     1681 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     1741 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     1801 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     1861 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     1921 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     1981 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     2041 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     2101 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     2161 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggt
//
