LOCUS       Exported                2801 bp ds-DNA     circular SYN 22-MAR-2024
DEFINITION  Generated by Gibson/Homology Cloning (DVLA backbone w CDS overhangs,
            TurboRFP (CDS)).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Apr 11, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1
                     /label=Ligation
     misc_feature    join(2..2801,1)
                     /label=ATG
     misc_feature    2..697
                     /locus_tag="TurboRFP sequence"
                     /label=TurboRFP sequence
     primer_bind     2..22
                     /label=TurboRFP (CDS) (1 F) - DVLA backbone w CDS over...
     primer_bind     complement(600..628)
                     /label=remove_BsaI_pt2_R
     primer_bind     625..648
                     /label=remove_BsaI_pt1_F
     misc_feature    625
                     /label=G
     misc_feature    628
                     /label=C
     primer_bind     complement(678..697)
                     /label=TurboRFP (CDS) (696 R) - DVLA backbone w CDS ov...
     misc_feature    698..2802
                     /label=DVLA backbone w CDS overhangs (concatenated seq...
     misc_feature    698..715
                     /label=Ligation
     misc_feature    702..2801
                     /label=DVLA
     misc_feature    716..2801
                     /label=Extracted region from C4d
     primer_bind     complement(785..805)
                     /label=SC190/Part.Seq.REV
     CDS             complement(1665..2525)
                     /label=bla
     primer_bind     2000..2020
                     /label=remove_BsaI_pt2_F
     primer_bind     complement(2000..2020)
                     /label=remove_BsaI_pt1_R
     primer_bind     2697..2717
                     /label=SC189/Part.Seq.FOR
     misc_feature    2788..3516
                     /label=TurboRFP (CDS) (concatenated sequence 2)
     misc_feature    2788..2801
                     /label=Ligation
ORIGIN
        1 aatgagcgag ctgatcaagg agaacatgca catgaagctg tacatggagg gcaccgtgaa
       61 caaccaccac ttcaagtgca catccgaggg cgaaggcaag ccctacgagg gcacccagac
      121 catgaagatc aaggtggtcg agggcggccc tctccccttc gccttcgaca tcctggctac
      181 cagcttcatg tacggcagca aagccttcat caaccacacc cagggcatcc ccgacttctt
      241 taagcagtcc ttccctgagg gcttcacatg ggagagaatc accacatacg aagacggggg
      301 cgtgctgacc gctacccagg acaccagctt ccagaacggc tgcatcatct acaacgtcaa
      361 gatcaacggg gtgaacttcc catccaacgg ccctgtgatg cagaagaaaa cacgcggctg
      421 ggaggccaac accgagatgc tgtaccccgc tgacggcggc ctgagaggcc acagccagat
      481 ggccctgaag ctcgtgggcg ggggctacct gcactgctcc ttcaagacca catacagatc
      541 caagaaaccc gctaagaacc tcaagatgcc cggcttccac ttcgtggacc acagactgga
      601 aagaatcaag gaggccgaca aagaaacgta cgtcgagcag cacgagatgg ctgtggccaa
      661 gtactgcgac ctccctagca aactggggca cagatgaagg tagagaccta ctagtagcgg
      721 ccgctgcagg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
      781 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
      841 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
      901 gcgtttttcc acaggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
      961 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     1021 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     1081 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     1141 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     1201 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     1261 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     1321 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     1381 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     1441 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     1501 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     1561 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     1621 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     1681 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     1741 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     1801 ccgcgcgacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     1861 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     1921 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     1981 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     2041 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     2101 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     2161 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     2221 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     2281 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     2341 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     2401 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     2461 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     2521 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     2581 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     2641 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
     2701 aggcgtatca cgaggcagaa tttcagataa aaaaaatcct tagctttcgc taaggatgat
     2761 ttctggaatt cgcggccgct tctagagact agtgggtctc a
//
