LOCUS       Exported                6992 bp ds-DNA     circular SYN 06-SEP-2024
DEFINITION  Gibson/Homology Cloning ligation product.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Apr 11, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6992
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..4115
                     /label=EML002 backbone (fEL077+fEL076) extraction (con...
     misc_feature    1..4115
                     /label=Extracted region from EML002
     CDS             complement(171..1031)
                     /label=AmpR
     promoter        complement(1032..1136)
                     /label=AmpR promoter
     primer_bind     1176..1195
                     /label=oEL180
     mobile_element  complement(1210..1375)
                     /label=transposon:Tn7
     misc_feature    1475..4076
                     /label=oEL172 + oEL159 (concatenated sequence 2)
     misc_feature    1475..1504
                     /label=Ligation
     primer_bind     complement(1475..1504)
                     /label=oEL173
     primer_bind     1475..1504
                     /label=oEL172
     primer_bind     complement(1475..1504)
                     /label=oEL173
     primer_bind     1475..1504
                     /label=oEL172
     primer_bind     complement(1501..1523)
                     /label=oEL236
     CDS             1507..2322
                     /label=KanR
     primer_bind     complement(1717..1736)
                     /label=oEL179
     terminator      2701..2732
                     /label=T4-32
     primer_bind     3660..3679
                     /label=oEL178
     terminator      3756..3787
                     /label=T4-32
     promoter        complement(3891..3968)
                     /label=AmpR promoter
     primer_bind     3994..4022
                     /label=oEL245
     misc_feature    4044..4076
                     /label=Ligation
     primer_bind     complement(4049..4068)
                     /label=oEL182
     primer_bind     complement(4055..4076)
                     /label=3G_bb_R
     primer_bind     complement(4056..4076)
                     /label=oEL159
     misc_feature    4077..5020
                     /label=fOW002 (concatenated sequence 2)
     misc_feature    4077..4120
                     /label=UNS1_A (concatenated sequence 1)
     primer_bind     4077..4116
                     /label=UNS1
     misc_feature    4077..4115
                     /label=Ligation
     misc_feature    4077..4115
                     /label=UNS1
     primer_bind     4077..4115
                     /label=UNS1_F
     primer_bind     complement(4077..4115)
                     /label=UNS1_R
     misc_feature    4116
                     /label=Extracted region from fOW002
     misc_feature    4117..4980
                     /label=P1m-U3d-TurboRFP-t0 (concatenated sequence 2)
     misc_feature    4117..4159
                     /label=P1m (concatenated sequence 1)
     misc_feature    4117..4159
                     /label=Editing History Insertion
     misc_feature    4117..4155
                     /label=Extracted region from P1m
     misc_feature    4117..4120
                     /label=Ligation
     primer_bind     4117..4120
                     /label=Binds to A sticky end
     promoter        4121..4155
                     
     misc_feature    4156..4184
                     /label=U3d (concatenated sequence 2)
     misc_feature    4156..4180
                     /label=Extracted region from U3d
     misc_feature    4156..4159
                     /label=Ligation
     RBS             4160..4180
                     /label=B0034m
     5'UTR           4164..4175
                     /label=B0034
     misc_feature    4181..4881
                     /label=TurboRFP (CDS) - removed BsaI cut-site (concate...
     misc_feature    4181..4881
                     /label=TurboRFP (CDS) (concatenated sequence 2)
     misc_feature    4181..4877
                     /label=Extracted region from TurboRFP (CDS) - removed ...
     misc_feature    4181..4184
                     /label=Ligation
     misc_feature    4181
                     /label=Ligation
     misc_feature    4182..4877
                     /locus_tag="TurboRFP sequence"
                     /label=TurboRFP sequence
     primer_bind     4182..4202
                     /label=TurboRFP (CDS) (1 F) - DVLA backbone w CDS over...
     primer_bind     complement(4780..4808)
                     /label=remove_BsaI_pt2_R
     primer_bind     4805..4828
                     /label=remove_BsaI_pt1_F
     misc_feature    4805
                     /label=G
     misc_feature    4808
                     /label=C
     primer_bind     complement(4858..4877)
                     /label=TurboRFP (CDS) (696 R) - DVLA backbone w CDS ov...
     misc_feature    join(4878..4881,4181)
                     /label=DVLA backbone w CDS overhangs (concatenated seq...
     misc_feature    4878..4980
                     /label=DVLA backbone w terminator overhangs - EML001 w...
     misc_feature    4878..4980
                     /label=EML001 with UNS - lambda t0 terminator (concate...
     misc_feature    4878..4881
                     /label=Ligation
     misc_feature    4878..4881
                     /label=Ligation
     misc_feature    4878..4881
                     /label=Ligation
     misc_feature    4878..4881
                     /label=Extracted region from T2m
     terminator      complement(4882..4976)
                     /label=lambda t0 terminator
     misc_feature    4882..4976
                     /label=Extracted region from EML001 with UNS
     primer_bind     4882..4903
                     /label=EML001 with UNS - lambda t0 terminator (1 F) - ...
     primer_bind     complement(4953..4976)
                     /label=EML001 with UNS - lambda t0 terminator (95 R) -...
     misc_feature    join(4977..4980,4878..4881)
                     /label=DVLA backbone w terminator overhangs (concatena...
     misc_feature    4977..5020
                     /label=UNSX_E (concatenated sequence 3)
     misc_feature    4977..5020
                     /label=Extracted region from UNSX_E
     misc_feature    4977..4980
                     /label=Ligation
     misc_feature    4977..4980
                     /label=Ligation
     primer_bind     4977..4980
                     /label=Binds to E sticky end
     misc_feature    join(4981..5050,4044..4115)
                     /label=fEL042 (concatenated sequence 3)
     misc_feature    4981..6992
                     /label=EML002 backbone (fEL077+fEL076) extraction (con...
     misc_feature    4981..5020
                     /label=Ligation
     primer_bind     4981..5020
                     /label=UNS10
     misc_feature    4981..5020
                     /label=UNSX
     primer_bind     4981..5020
                     /label=UNSX_F
     misc_feature    5021..8496
                     /label=oEL171 + oEL173 (concatenated sequence 1)
     misc_feature    5021..5050
                     /label=Extracted region from fEL042
     misc_feature    5021..5050
                     /label=Ligation
     primer_bind     5021..5042
                     /label=oEL171
     misc_feature    5051..6992
                     /label=Extracted region from EML001 with UNS
     mobile_element  complement(5101..5299)
                     /label=transposon:Tn7
     primer_bind     complement(5308..5329)
                     /label=oEL188
     primer_bind     5309..5328
                     /label=oEL181
     rep_origin      complement(5381..5769)
                     /direction=LEFT
                     /label=R6K gamma ori
     CDS             5766..5795
                     /label=TcR
     CDS             complement(5940..6311)
                     /label=traJ
     oriT            complement(6344..6453)
                     /direction=LEFT
     rep_origin      complement(6587..6992)
                     /direction=LEFT
                     /label=ori
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc tgaattaatt cgagctcgcc caaacataac aggaagaaaa
     1201 atgccccgct gtgggcggac aaaatagttg ggaactggga ggggtggaaa tggagttttt
     1261 aaggattatt tagggaagag tgacaaaata gatgggaact gggtgtagcg tcgtaagcta
     1321 atacgaaaat taaaaatgac aaaatagttt ggaactagat ttcacttatc tggttggcct
     1381 gcaggggggg ggggggaaag ccacgttgtg tctcaaaatc tctgatgtta cattgcacaa
     1441 gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg
     1501 ggtgttatga gccatattca acgggaaacg tcttgctcga ggccgcgatt aaattccaac
     1561 atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg
     1621 acaatctatc gattgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa
     1681 ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt
     1741 atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc
     1801 actgcgatcc ccgggaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa
     1861 aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat
     1921 tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac
     1981 ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc
     2041 tggaaagaaa tgcataagct tttgccattc tcaccggatt cagtcgtcac tcatggtgat
     2101 ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga
     2161 cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag
     2221 ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg
     2281 aataaattgc agtttcattt gatgctcgat gagtttttct aatcagaatt ggttaattgg
     2341 ttgtaacact ggcagagcat tacgctgact tgacgggacg gcggctttgt tgaataaatc
     2401 gaacttttgc tgagttgaag gatcagatca cgcatcttcc cgacaacgca gaccgttccg
     2461 tggcaaagca aaagttcaaa atcaccaact ggtccaccta caacaaagct ctcatcaacc
     2521 gtggctccct cactttctgg ctggatgatg gggcgattca ggcctggtat gagtcagcaa
     2581 caccttcttc acgaggcaga cctcagcgcc cccccccccc tgcaggtcga ctagagtcgg
     2641 ggatccggtg attgattgag caagctttat gcttgtaaac cgttttgtga aaaaattttt
     2701 aaaataaaaa aggggacctc tagggtcccc aattaattag taatataatc tattaaaggt
     2761 cattcaaaag gtcatccacc ggatcaattc ccctgctcgc gcaggctggg tgccaagctc
     2821 tcgggtaaca tcaaggcccg atccttggag cccttgccct cccgcacgat gatcgtgccg
     2881 tgatcgaaat ccagatcctt gacccgcagt tgcaaaccct cactgatccg catgcccgtt
     2941 ccatacagaa gctgggcgaa caaacgatgc tcgccttcca gaaaaccgag gatgcgaacc
     3001 acttcatccg gggtcagcac caccggcaag cgccgcgacg gccgaggtct tccgatctcc
     3061 tgaagccagg gcagatccgt gcacagcacc ttgccgtaga agaacagcaa ggccgccaat
     3121 gcctgacgat gcgtggagac cgaaaccttg cgctcgttcg ccagccagga cagaaatgcc
     3181 tcgacttcgc tgctgcccaa ggttgccggg tgacgcacac cgtggaaacg gatgaaggca
     3241 cgaacccagt ggacataagc ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc
     3301 tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt
     3361 ttcatggctt gttatgactg tttttttggg gtacagtcta tgcctcgggc atccaagcag
     3421 caagcgcgtt acgccgtggg tcgatgtttg tgtctaacaa ttcgttcaag ccgacgccgc
     3481 ttcgcggcgc ggcttaactc aagcgttaga tgcactaagc acataattgc tcacagccaa
     3541 actatcaggt caagtctgct tttattattt ttaagcgtgc ataataagcc ctacacaaat
     3601 tgggagatat atcatgaaag gctggctttt tcttgttatc gcaatagttg gcgaagtaat
     3661 cgcaacatcc gcattaaaat ctagcgaggg ctttactaag ctgatccggt ggatgacctt
     3721 ttgaatgacc tttaatagat tatattacta attaattggg gaccctagag gtcccctttt
     3781 ttattttaaa aattttttca caaaacggtt tacaagcata aagcttgctc aatcaatcac
     3841 cggatcccga cgggcccggt accgcggccg cgcgaattcg agctcggtac catttatcag
     3901 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     3961 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     4021 acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcacct cgagaacatt
     4081 actcgcatcc attctcaggc tgtctcgtct cgtctcggag tttacagcta gctcagtcct
     4141 aggtattatg ctagctacta gagaaagagg agaaatacta aatgagcgag ctgatcaagg
     4201 agaacatgca catgaagctg tacatggagg gcaccgtgaa caaccaccac ttcaagtgca
     4261 catccgaggg cgaaggcaag ccctacgagg gcacccagac catgaagatc aaggtggtcg
     4321 agggcggccc tctccccttc gccttcgaca tcctggctac cagcttcatg tacggcagca
     4381 aagccttcat caaccacacc cagggcatcc ccgacttctt taagcagtcc ttccctgagg
     4441 gcttcacatg ggagagaatc accacatacg aagacggggg cgtgctgacc gctacccagg
     4501 acaccagctt ccagaacggc tgcatcatct acaacgtcaa gatcaacggg gtgaacttcc
     4561 catccaacgg ccctgtgatg cagaagaaaa cacgcggctg ggaggccaac accgagatgc
     4621 tgtaccccgc tgacggcggc ctgagaggcc acagccagat ggccctgaag ctcgtgggcg
     4681 ggggctacct gcactgctcc ttcaagacca catacagatc caagaaaccc gctaagaacc
     4741 tcaagatgcc cggcttccac ttcgtggacc acagactgga aagaatcaag gaggccgaca
     4801 aagaaacgta cgtcgagcag cacgagatgg ctgtggccaa gtactgcgac ctccctagca
     4861 aactggggca cagatgaagg tgactcctgt tgatagatcc agtaatgacc tcagaactcc
     4921 atctggattt gttcagaacg ctcggttgcc gccgggcgtt ttttattggt gagaatgctt
     4981 ccaggataca tagattacca caactccgag cccttccacc ccaagctagc ttggaggaca
     5041 aatccgccgc taggagcttg cggccgcctt aagacgcgtg gatccccggg aatttcgacc
     5101 ccacgcccct ctttaatacg acgggcaatt tgcacttcag aaaatgaaga gtttgcttta
     5161 gccataacaa aagtccagta tgctttttca cagcataact ggactgattt cagtttacaa
     5221 ctattctgtc tagtttaaga ctttattgtc atagtttaga tctattttgt tcagtttaag
     5281 actttattgt ccgcccacac ccgcttacgc agggcatcca tttattactc aaccgtaacc
     5341 gattttgcca ggttacgcgg ctggtcctct agaattccca tgtcagccgt taagtgttcc
     5401 tgtgtcactc aaaattgctt tgagaggctc taagggcttc tcagtgcgtt acatccctgg
     5461 cttgttgtcc acaaccgtta aaccttaaaa gctttaaaag ccttatatat tctttttttt
     5521 cttataaaac ttaaaacctt agaggctatt taagttgctg atttatatta attttattgt
     5581 tcaaacatga gagcttagta cgtgaaacat gagagcttag tacgttagcc atgagagctt
     5641 agtacgttag ccatgagggt ttagttcgtt aaacatgaga gcttagtacg ttaaacatga
     5701 gagcttagta cgtgaaacat gagagcttag tacgtactat caacaggttg aactgctgat
     5761 cttcagatcc tctacgccgg acgcatcgtg gccggatcgg gggggggggg cgctcggtct
     5821 tgccttgctc gtcggtgatg tacttcacca gctccgcgaa gtcgctcttc ttgatggagc
     5881 gcatggggac gtgcttggca atcacgcgca ccccccggcc gttttagcgg ctaaaaaagt
     5941 catggctctg ccctcgggcg gaccacgccc atcatgacct tgccaagctc gtcctgcttc
     6001 tcttcgatct tcgccagcag ggcgaggatc gtggcatcac cgaaccgcgc cgtgcgcggg
     6061 tcgtcggtga gccagagttt cagcaggccg cccaggcggc ccaggtcgcc attgatgcgg
     6121 gccagctcgc ggacgtgctc atagtccacg acgcccgtga ttttgtagcc ctggccgacg
     6181 gccagcaggt aggccgacag gctcatgccg gccgccgccg ccttttcctc aatcgctctt
     6241 cgttcgtctg gaaggcagta caccttgata ggtgggctgc ccttcctggt tggcttggtt
     6301 tcatcagcca tccgcttgcc ctcatctgtt acgccggcgg tagccggcca gcctcgcaga
     6361 gcaggattcc cgttgagcac cgccaggtgc gaataaggga cagtgaagaa ggaacacccg
     6421 ctcgcgggtg ggcctacttc acctatcctg cccggctgac gccgttggat acaccaagga
     6481 aagtctacac gaaccctttg gcaaaatcct gtatatcgtg cgaaaaagga tggatatacc
     6541 gaaaaaatcg ctataatgac cccgaagcag ggttatgcag cggaaaagcg ctttctcata
     6601 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     6661 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     6721 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     6781 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     6841 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     6901 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     6961 agcagattac gcgcagaaaa aaaggatctc aa
//
