LOCUS       Exported                7055 bp ds-DNA     circular SYN 06-SEP-2024
DEFINITION  Gibson/Homology Cloning ligation product.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7055)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Apr 11, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7055
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..4115
                     /label=EML002 backbone (fEL077+fEL076) extraction (con...
     misc_feature    1..4115
                     /label=Extracted region from EML002
     CDS             complement(171..1031)
                     /label=AmpR
     promoter        complement(1032..1136)
                     /label=AmpR promoter
     primer_bind     1176..1195
                     /label=oEL180
     mobile_element  complement(1210..1375)
                     /label=transposon:Tn7
     misc_feature    1475..4076
                     /label=oEL172 + oEL159 (concatenated sequence 2)
     misc_feature    1475..1504
                     /label=Ligation
     primer_bind     complement(1475..1504)
                     /label=oEL173
     primer_bind     1475..1504
                     /label=oEL172
     primer_bind     complement(1475..1504)
                     /label=oEL173
     primer_bind     1475..1504
                     /label=oEL172
     primer_bind     complement(1501..1523)
                     /label=oEL236
     CDS             1507..2322
                     /label=KanR
     primer_bind     complement(1717..1736)
                     /label=oEL179
     terminator      2701..2732
                     /label=T4-32
     primer_bind     3660..3679
                     /label=oEL178
     terminator      3756..3787
                     /label=T4-32
     promoter        complement(3891..3968)
                     /label=AmpR promoter
     primer_bind     3994..4022
                     /label=oEL245
     misc_feature    4044..4076
                     /label=Ligation
     primer_bind     complement(4049..4068)
                     /label=oEL182
     primer_bind     complement(4055..4076)
                     /label=3G_bb_R
     primer_bind     complement(4056..4076)
                     /label=oEL159
     misc_feature    4077..5083
                     /label=fOW023 (concatenated sequence 2)
     misc_feature    4077..4120
                     /label=UNS1_A (concatenated sequence 1)
     primer_bind     4077..4116
                     /label=UNS1
     misc_feature    4077..4115
                     /label=Ligation
     misc_feature    4077..4115
                     /label=UNS1
     primer_bind     4077..4115
                     /label=UNS1_F
     primer_bind     complement(4077..4115)
                     /label=UNS1_R
     misc_feature    4116
                     /label=Extracted region from fOW023
     misc_feature    4117..5043
                     /label=P2m-U5d-TurboRFP-t0 (concatenated sequence 2)
     misc_feature    4117..4159
                     /label=P2m (MAYBE) (concatenated sequence 1)
     misc_feature    4117..4159
                     /label=Editing History Insertion
     misc_feature    4117..4155
                     /label=Extracted region from P2m (MAYBE)
     misc_feature    4117..4120
                     /label=Ligation
     primer_bind     4117..4120
                     /label=Binds to A sticky end
     promoter        4121..4155
                     /label=J23119
     misc_feature    4156..4247
                     /label=U5d (concatenated sequence 2)
     misc_feature    4156..4243
                     /label=Extracted region from U5d
     misc_feature    4156..4159
                     /label=Ligation
     5'UTR           4160..4243
                     /label=BCD8
     misc_feature    4244..4944
                     /label=TurboRFP (CDS) - removed BsaI cut-site (concate...
     misc_feature    4244..4944
                     /label=TurboRFP (CDS) (concatenated sequence 2)
     misc_feature    4244..4940
                     /label=Extracted region from TurboRFP (CDS) - removed ...
     misc_feature    4244..4247
                     /label=Ligation
     misc_feature    4244
                     /label=Ligation
     misc_feature    4245..4940
                     /locus_tag="TurboRFP sequence"
                     /label=TurboRFP sequence
     primer_bind     4245..4265
                     /label=TurboRFP (CDS) (1 F) - DVLA backbone w CDS over...
     primer_bind     complement(4843..4871)
                     /label=remove_BsaI_pt2_R
     primer_bind     4868..4891
                     /label=remove_BsaI_pt1_F
     misc_feature    4868
                     /label=G
     misc_feature    4871
                     /label=C
     primer_bind     complement(4921..4940)
                     /label=TurboRFP (CDS) (696 R) - DVLA backbone w CDS ov...
     misc_feature    join(4941..4944,4244)
                     /label=DVLA backbone w CDS overhangs (concatenated seq...
     misc_feature    4941..5043
                     /label=DVLA backbone w terminator overhangs - EML001 w...
     misc_feature    4941..5043
                     /label=EML001 with UNS - lambda t0 terminator (concate...
     misc_feature    4941..4944
                     /label=Ligation
     misc_feature    4941..4944
                     /label=Ligation
     misc_feature    4941..4944
                     /label=Ligation
     misc_feature    4941..4944
                     /label=Extracted region from T2m
     terminator      complement(4945..5039)
                     /label=lambda t0 terminator
     misc_feature    4945..5039
                     /label=Extracted region from EML001 with UNS
     primer_bind     4945..4966
                     /label=EML001 with UNS - lambda t0 terminator (1 F) - ...
     primer_bind     complement(5016..5039)
                     /label=EML001 with UNS - lambda t0 terminator (95 R) -...
     misc_feature    join(5040..5043,4941..4944)
                     /label=DVLA backbone w terminator overhangs (concatena...
     misc_feature    5040..5083
                     /label=UNSX_E (concatenated sequence 3)
     misc_feature    5040..5083
                     /label=Extracted region from UNSX_E
     misc_feature    5040..5043
                     /label=Ligation
     misc_feature    5040..5043
                     /label=Ligation
     primer_bind     5040..5043
                     /label=Binds to E sticky end
     misc_feature    join(5044..5113,4044..4115)
                     /label=fEL042 (concatenated sequence 3)
     misc_feature    5044..7055
                     /label=EML002 backbone (fEL077+fEL076) extraction (con...
     misc_feature    5044..5083
                     /label=Ligation
     primer_bind     5044..5083
                     /label=UNS10
     misc_feature    5044..5083
                     /label=UNSX
     primer_bind     5044..5083
                     /label=UNSX_F
     misc_feature    5084..8559
                     /label=oEL171 + oEL173 (concatenated sequence 1)
     misc_feature    5084..5113
                     /label=Extracted region from fEL042
     misc_feature    5084..5113
                     /label=Ligation
     primer_bind     5084..5105
                     /label=oEL171
     misc_feature    5114..7055
                     /label=Extracted region from EML001 with UNS
     mobile_element  complement(5164..5362)
                     /label=transposon:Tn7
     primer_bind     complement(5371..5392)
                     /label=oEL188
     primer_bind     5372..5391
                     /label=oEL181
     rep_origin      complement(5444..5832)
                     /direction=LEFT
                     /label=R6K gamma ori
     CDS             5829..5858
                     /label=TcR
     CDS             complement(6003..6374)
                     /label=traJ
     oriT            complement(6407..6516)
                     /direction=LEFT
     rep_origin      complement(6650..7055)
                     /direction=LEFT
                     /label=ori
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc tgaattaatt cgagctcgcc caaacataac aggaagaaaa
     1201 atgccccgct gtgggcggac aaaatagttg ggaactggga ggggtggaaa tggagttttt
     1261 aaggattatt tagggaagag tgacaaaata gatgggaact gggtgtagcg tcgtaagcta
     1321 atacgaaaat taaaaatgac aaaatagttt ggaactagat ttcacttatc tggttggcct
     1381 gcaggggggg ggggggaaag ccacgttgtg tctcaaaatc tctgatgtta cattgcacaa
     1441 gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg
     1501 ggtgttatga gccatattca acgggaaacg tcttgctcga ggccgcgatt aaattccaac
     1561 atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg
     1621 acaatctatc gattgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa
     1681 ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt
     1741 atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc
     1801 actgcgatcc ccgggaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa
     1861 aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat
     1921 tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac
     1981 ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc
     2041 tggaaagaaa tgcataagct tttgccattc tcaccggatt cagtcgtcac tcatggtgat
     2101 ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga
     2161 cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag
     2221 ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg
     2281 aataaattgc agtttcattt gatgctcgat gagtttttct aatcagaatt ggttaattgg
     2341 ttgtaacact ggcagagcat tacgctgact tgacgggacg gcggctttgt tgaataaatc
     2401 gaacttttgc tgagttgaag gatcagatca cgcatcttcc cgacaacgca gaccgttccg
     2461 tggcaaagca aaagttcaaa atcaccaact ggtccaccta caacaaagct ctcatcaacc
     2521 gtggctccct cactttctgg ctggatgatg gggcgattca ggcctggtat gagtcagcaa
     2581 caccttcttc acgaggcaga cctcagcgcc cccccccccc tgcaggtcga ctagagtcgg
     2641 ggatccggtg attgattgag caagctttat gcttgtaaac cgttttgtga aaaaattttt
     2701 aaaataaaaa aggggacctc tagggtcccc aattaattag taatataatc tattaaaggt
     2761 cattcaaaag gtcatccacc ggatcaattc ccctgctcgc gcaggctggg tgccaagctc
     2821 tcgggtaaca tcaaggcccg atccttggag cccttgccct cccgcacgat gatcgtgccg
     2881 tgatcgaaat ccagatcctt gacccgcagt tgcaaaccct cactgatccg catgcccgtt
     2941 ccatacagaa gctgggcgaa caaacgatgc tcgccttcca gaaaaccgag gatgcgaacc
     3001 acttcatccg gggtcagcac caccggcaag cgccgcgacg gccgaggtct tccgatctcc
     3061 tgaagccagg gcagatccgt gcacagcacc ttgccgtaga agaacagcaa ggccgccaat
     3121 gcctgacgat gcgtggagac cgaaaccttg cgctcgttcg ccagccagga cagaaatgcc
     3181 tcgacttcgc tgctgcccaa ggttgccggg tgacgcacac cgtggaaacg gatgaaggca
     3241 cgaacccagt ggacataagc ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc
     3301 tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt
     3361 ttcatggctt gttatgactg tttttttggg gtacagtcta tgcctcgggc atccaagcag
     3421 caagcgcgtt acgccgtggg tcgatgtttg tgtctaacaa ttcgttcaag ccgacgccgc
     3481 ttcgcggcgc ggcttaactc aagcgttaga tgcactaagc acataattgc tcacagccaa
     3541 actatcaggt caagtctgct tttattattt ttaagcgtgc ataataagcc ctacacaaat
     3601 tgggagatat atcatgaaag gctggctttt tcttgttatc gcaatagttg gcgaagtaat
     3661 cgcaacatcc gcattaaaat ctagcgaggg ctttactaag ctgatccggt ggatgacctt
     3721 ttgaatgacc tttaatagat tatattacta attaattggg gaccctagag gtcccctttt
     3781 ttattttaaa aattttttca caaaacggtt tacaagcata aagcttgctc aatcaatcac
     3841 cggatcccga cgggcccggt accgcggccg cgcgaattcg agctcggtac catttatcag
     3901 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     3961 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     4021 acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcacct cgagaacatt
     4081 actcgcatcc attctcaggc tgtctcgtct cgtctcggag ttgacggcta gctcagtcct
     4141 aggtacagtg ctagctactg ggcccaagtt cacttaaaaa ggagatcaac aatgaaagca
     4201 attttcgtac tgaaacatct taatcatgca tcggaccgtt tctaatgagc gagctgatca
     4261 aggagaacat gcacatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt
     4321 gcacatccga gggcgaaggc aagccctacg agggcaccca gaccatgaag atcaaggtgg
     4381 tcgagggcgg ccctctcccc ttcgccttcg acatcctggc taccagcttc atgtacggca
     4441 gcaaagcctt catcaaccac acccagggca tccccgactt ctttaagcag tccttccctg
     4501 agggcttcac atgggagaga atcaccacat acgaagacgg gggcgtgctg accgctaccc
     4561 aggacaccag cttccagaac ggctgcatca tctacaacgt caagatcaac ggggtgaact
     4621 tcccatccaa cggccctgtg atgcagaaga aaacacgcgg ctgggaggcc aacaccgaga
     4681 tgctgtaccc cgctgacggc ggcctgagag gccacagcca gatggccctg aagctcgtgg
     4741 gcgggggcta cctgcactgc tccttcaaga ccacatacag atccaagaaa cccgctaaga
     4801 acctcaagat gcccggcttc cacttcgtgg accacagact ggaaagaatc aaggaggccg
     4861 acaaagaaac gtacgtcgag cagcacgaga tggctgtggc caagtactgc gacctcccta
     4921 gcaaactggg gcacagatga aggtgactcc tgttgataga tccagtaatg acctcagaac
     4981 tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatt ggtgagaatg
     5041 cttccaggat acatagatta ccacaactcc gagcccttcc accccaagct agcttggagg
     5101 acaaatccgc cgctaggagc ttgcggccgc cttaagacgc gtggatcccc gggaatttcg
     5161 accccacgcc cctctttaat acgacgggca atttgcactt cagaaaatga agagtttgct
     5221 ttagccataa caaaagtcca gtatgctttt tcacagcata actggactga tttcagttta
     5281 caactattct gtctagttta agactttatt gtcatagttt agatctattt tgttcagttt
     5341 aagactttat tgtccgccca cacccgctta cgcagggcat ccatttatta ctcaaccgta
     5401 accgattttg ccaggttacg cggctggtcc tctagaattc ccatgtcagc cgttaagtgt
     5461 tcctgtgtca ctcaaaattg ctttgagagg ctctaagggc ttctcagtgc gttacatccc
     5521 tggcttgttg tccacaaccg ttaaacctta aaagctttaa aagccttata tattcttttt
     5581 tttcttataa aacttaaaac cttagaggct atttaagttg ctgatttata ttaattttat
     5641 tgttcaaaca tgagagctta gtacgtgaaa catgagagct tagtacgtta gccatgagag
     5701 cttagtacgt tagccatgag ggtttagttc gttaaacatg agagcttagt acgttaaaca
     5761 tgagagctta gtacgtgaaa catgagagct tagtacgtac tatcaacagg ttgaactgct
     5821 gatcttcaga tcctctacgc cggacgcatc gtggccggat cggggggggg gggcgctcgg
     5881 tcttgccttg ctcgtcggtg atgtacttca ccagctccgc gaagtcgctc ttcttgatgg
     5941 agcgcatggg gacgtgcttg gcaatcacgc gcaccccccg gccgttttag cggctaaaaa
     6001 agtcatggct ctgccctcgg gcggaccacg cccatcatga ccttgccaag ctcgtcctgc
     6061 ttctcttcga tcttcgccag cagggcgagg atcgtggcat caccgaaccg cgccgtgcgc
     6121 gggtcgtcgg tgagccagag tttcagcagg ccgcccaggc ggcccaggtc gccattgatg
     6181 cgggccagct cgcggacgtg ctcatagtcc acgacgcccg tgattttgta gccctggccg
     6241 acggccagca ggtaggccga caggctcatg ccggccgccg ccgccttttc ctcaatcgct
     6301 cttcgttcgt ctggaaggca gtacaccttg ataggtgggc tgcccttcct ggttggcttg
     6361 gtttcatcag ccatccgctt gccctcatct gttacgccgg cggtagccgg ccagcctcgc
     6421 agagcaggat tcccgttgag caccgccagg tgcgaataag ggacagtgaa gaaggaacac
     6481 ccgctcgcgg gtgggcctac ttcacctatc ctgcccggct gacgccgttg gatacaccaa
     6541 ggaaagtcta cacgaaccct ttggcaaaat cctgtatatc gtgcgaaaaa ggatggatat
     6601 accgaaaaaa tcgctataat gaccccgaag cagggttatg cagcggaaaa gcgctttctc
     6661 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     6721 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     6781 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     6841 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     6901 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     6961 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     7021 agcagcagat tacgcgcaga aaaaaaggat ctcaa
//
