LOCUS       Exported                7130 bp ds-DNA     circular SYN 06-SEP-2024
DEFINITION  Gibson/Homology Cloning ligation product.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7130)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Apr 11, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7130
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..4115
                     /label=EML002 backbone (fEL077+fEL076) extraction (con...
     misc_feature    1..4115
                     /label=Extracted region from EML002
     CDS             complement(171..1031)
                     /label=AmpR
     promoter        complement(1032..1136)
                     /label=AmpR promoter
     primer_bind     1176..1195
                     /label=oEL180
     mobile_element  complement(1210..1375)
                     /label=transposon:Tn7
     misc_feature    1475..4076
                     /label=oEL172 + oEL159 (concatenated sequence 2)
     misc_feature    1475..1504
                     /label=Ligation
     primer_bind     complement(1475..1504)
                     /label=oEL173
     primer_bind     1475..1504
                     /label=oEL172
     primer_bind     complement(1475..1504)
                     /label=oEL173
     primer_bind     1475..1504
                     /label=oEL172
     primer_bind     complement(1501..1523)
                     /label=oEL236
     CDS             1507..2322
                     /label=KanR
     primer_bind     complement(1717..1736)
                     /label=oEL179
     terminator      2701..2732
                     /label=T4-32
     primer_bind     3660..3679
                     /label=oEL178
     terminator      3756..3787
                     /label=T4-32
     promoter        complement(3891..3968)
                     /label=AmpR promoter
     primer_bind     3994..4022
                     /label=oEL245
     misc_feature    4044..4076
                     /label=Ligation
     primer_bind     complement(4049..4068)
                     /label=oEL182
     primer_bind     complement(4055..4076)
                     /label=3G_bb_R
     primer_bind     complement(4056..4076)
                     /label=oEL159
     misc_feature    4077..5158
                     /label=fOW026 (concatenated sequence 2)
     misc_feature    4077..4120
                     /label=UNS1_A (concatenated sequence 1)
     primer_bind     4077..4116
                     /label=UNS1
     misc_feature    4077..4115
                     /label=Ligation
     misc_feature    4077..4115
                     /label=UNS1
     primer_bind     4077..4115
                     /label=UNS1_F
     primer_bind     complement(4077..4115)
                     /label=UNS1_R
     misc_feature    4116
                     /label=Extracted region from fOW026
     misc_feature    4117..5118
                     /label=P2m-U24m-TurboRFP-t0 (concatenated sequence 2)
     misc_feature    4117..4159
                     /label=P2m (MAYBE) (concatenated sequence 1)
     misc_feature    4117..4159
                     /label=Editing History Insertion
     misc_feature    4117..4155
                     /label=Extracted region from P2m (MAYBE)
     misc_feature    4117..4120
                     /label=Ligation
     primer_bind     4117..4120
                     /label=Binds to A sticky end
     promoter        4121..4155
                     /label=J23119
     misc_feature    4156..4322
                     /label=U24m (concatenated sequence 2)
     misc_feature    4156..4318
                     /label=Extracted region from U24m
     misc_feature    4156..4159
                     /label=Ligation
     misc_RNA        4160..4234
                     /label=RiboJ
     misc_feature    4161..4317
                     /label=TGTCTTCTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAG...
     5'UTR           4235..4318
                     /label=BCD2
     misc_feature    4319..5019
                     /label=TurboRFP (CDS) - removed BsaI cut-site (concate...
     misc_feature    4319..5019
                     /label=TurboRFP (CDS) (concatenated sequence 2)
     misc_feature    4319..5015
                     /label=Extracted region from TurboRFP (CDS) - removed ...
     misc_feature    4319..4322
                     /label=Ligation
     misc_feature    4319
                     /label=Ligation
     misc_feature    4320..5015
                     /locus_tag="TurboRFP sequence"
                     /label=TurboRFP sequence
     primer_bind     4320..4340
                     /label=TurboRFP (CDS) (1 F) - DVLA backbone w CDS over...
     primer_bind     complement(4918..4946)
                     /label=remove_BsaI_pt2_R
     primer_bind     4943..4966
                     /label=remove_BsaI_pt1_F
     misc_feature    4943
                     /label=G
     misc_feature    4946
                     /label=C
     primer_bind     complement(4996..5015)
                     /label=TurboRFP (CDS) (696 R) - DVLA backbone w CDS ov...
     misc_feature    join(5016..5019,4319)
                     /label=DVLA backbone w CDS overhangs (concatenated seq...
     misc_feature    5016..5118
                     /label=DVLA backbone w terminator overhangs - EML001 w...
     misc_feature    5016..5118
                     /label=EML001 with UNS - lambda t0 terminator (concate...
     misc_feature    5016..5019
                     /label=Ligation
     misc_feature    5016..5019
                     /label=Ligation
     misc_feature    5016..5019
                     /label=Ligation
     misc_feature    5016..5019
                     /label=Extracted region from T2m
     terminator      complement(5020..5114)
                     /label=lambda t0 terminator
     misc_feature    5020..5114
                     /label=Extracted region from EML001 with UNS
     primer_bind     5020..5041
                     /label=EML001 with UNS - lambda t0 terminator (1 F) - ...
     primer_bind     complement(5091..5114)
                     /label=EML001 with UNS - lambda t0 terminator (95 R) -...
     misc_feature    join(5115..5118,5016..5019)
                     /label=DVLA backbone w terminator overhangs (concatena...
     misc_feature    5115..5158
                     /label=UNSX_E (concatenated sequence 3)
     misc_feature    5115..5158
                     /label=Extracted region from UNSX_E
     misc_feature    5115..5118
                     /label=Ligation
     misc_feature    5115..5118
                     /label=Ligation
     primer_bind     5115..5118
                     /label=Binds to E sticky end
     misc_feature    join(5119..5188,4044..4115)
                     /label=fEL042 (concatenated sequence 3)
     misc_feature    5119..7130
                     /label=EML002 backbone (fEL077+fEL076) extraction (con...
     misc_feature    5119..5158
                     /label=Ligation
     primer_bind     5119..5158
                     /label=UNS10
     misc_feature    5119..5158
                     /label=UNSX
     primer_bind     5119..5158
                     /label=UNSX_F
     misc_feature    5159..8634
                     /label=oEL171 + oEL173 (concatenated sequence 1)
     misc_feature    5159..5188
                     /label=Extracted region from fEL042
     misc_feature    5159..5188
                     /label=Ligation
     primer_bind     5159..5180
                     /label=oEL171
     misc_feature    5189..7130
                     /label=Extracted region from EML001 with UNS
     mobile_element  complement(5239..5437)
                     /label=transposon:Tn7
     primer_bind     complement(5446..5467)
                     /label=oEL188
     primer_bind     5447..5466
                     /label=oEL181
     rep_origin      complement(5519..5907)
                     /direction=LEFT
                     /label=R6K gamma ori
     CDS             5904..5933
                     /label=TcR
     CDS             complement(6078..6449)
                     /label=traJ
     oriT            complement(6482..6591)
                     /direction=LEFT
     rep_origin      complement(6725..7130)
                     /direction=LEFT
                     /label=ori
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc tgaattaatt cgagctcgcc caaacataac aggaagaaaa
     1201 atgccccgct gtgggcggac aaaatagttg ggaactggga ggggtggaaa tggagttttt
     1261 aaggattatt tagggaagag tgacaaaata gatgggaact gggtgtagcg tcgtaagcta
     1321 atacgaaaat taaaaatgac aaaatagttt ggaactagat ttcacttatc tggttggcct
     1381 gcaggggggg ggggggaaag ccacgttgtg tctcaaaatc tctgatgtta cattgcacaa
     1441 gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg
     1501 ggtgttatga gccatattca acgggaaacg tcttgctcga ggccgcgatt aaattccaac
     1561 atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg
     1621 acaatctatc gattgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa
     1681 ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt
     1741 atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc
     1801 actgcgatcc ccgggaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa
     1861 aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat
     1921 tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac
     1981 ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc
     2041 tggaaagaaa tgcataagct tttgccattc tcaccggatt cagtcgtcac tcatggtgat
     2101 ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga
     2161 cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag
     2221 ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg
     2281 aataaattgc agtttcattt gatgctcgat gagtttttct aatcagaatt ggttaattgg
     2341 ttgtaacact ggcagagcat tacgctgact tgacgggacg gcggctttgt tgaataaatc
     2401 gaacttttgc tgagttgaag gatcagatca cgcatcttcc cgacaacgca gaccgttccg
     2461 tggcaaagca aaagttcaaa atcaccaact ggtccaccta caacaaagct ctcatcaacc
     2521 gtggctccct cactttctgg ctggatgatg gggcgattca ggcctggtat gagtcagcaa
     2581 caccttcttc acgaggcaga cctcagcgcc cccccccccc tgcaggtcga ctagagtcgg
     2641 ggatccggtg attgattgag caagctttat gcttgtaaac cgttttgtga aaaaattttt
     2701 aaaataaaaa aggggacctc tagggtcccc aattaattag taatataatc tattaaaggt
     2761 cattcaaaag gtcatccacc ggatcaattc ccctgctcgc gcaggctggg tgccaagctc
     2821 tcgggtaaca tcaaggcccg atccttggag cccttgccct cccgcacgat gatcgtgccg
     2881 tgatcgaaat ccagatcctt gacccgcagt tgcaaaccct cactgatccg catgcccgtt
     2941 ccatacagaa gctgggcgaa caaacgatgc tcgccttcca gaaaaccgag gatgcgaacc
     3001 acttcatccg gggtcagcac caccggcaag cgccgcgacg gccgaggtct tccgatctcc
     3061 tgaagccagg gcagatccgt gcacagcacc ttgccgtaga agaacagcaa ggccgccaat
     3121 gcctgacgat gcgtggagac cgaaaccttg cgctcgttcg ccagccagga cagaaatgcc
     3181 tcgacttcgc tgctgcccaa ggttgccggg tgacgcacac cgtggaaacg gatgaaggca
     3241 cgaacccagt ggacataagc ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc
     3301 tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt
     3361 ttcatggctt gttatgactg tttttttggg gtacagtcta tgcctcgggc atccaagcag
     3421 caagcgcgtt acgccgtggg tcgatgtttg tgtctaacaa ttcgttcaag ccgacgccgc
     3481 ttcgcggcgc ggcttaactc aagcgttaga tgcactaagc acataattgc tcacagccaa
     3541 actatcaggt caagtctgct tttattattt ttaagcgtgc ataataagcc ctacacaaat
     3601 tgggagatat atcatgaaag gctggctttt tcttgttatc gcaatagttg gcgaagtaat
     3661 cgcaacatcc gcattaaaat ctagcgaggg ctttactaag ctgatccggt ggatgacctt
     3721 ttgaatgacc tttaatagat tatattacta attaattggg gaccctagag gtcccctttt
     3781 ttattttaaa aattttttca caaaacggtt tacaagcata aagcttgctc aatcaatcac
     3841 cggatcccga cgggcccggt accgcggccg cgcgaattcg agctcggtac catttatcag
     3901 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     3961 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     4021 acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcacct cgagaacatt
     4081 actcgcatcc attctcaggc tgtctcgtct cgtctcggag ttgacggcta gctcagtcct
     4141 aggtacagtg ctagctacta gctgtcaccg gatgtgcttt ccggtctgat gagtccgtga
     4201 ggacgaaaca gcctctacaa ataattttgt ttaagggccc aagttcactt aaaaaggaga
     4261 tcaacaatga aagcaatttt cgtactgaaa catcttaatc atgctaagga ggttttctaa
     4321 tgagcgagct gatcaaggag aacatgcaca tgaagctgta catggagggc accgtgaaca
     4381 accaccactt caagtgcaca tccgagggcg aaggcaagcc ctacgagggc acccagacca
     4441 tgaagatcaa ggtggtcgag ggcggccctc tccccttcgc cttcgacatc ctggctacca
     4501 gcttcatgta cggcagcaaa gccttcatca accacaccca gggcatcccc gacttcttta
     4561 agcagtcctt ccctgagggc ttcacatggg agagaatcac cacatacgaa gacgggggcg
     4621 tgctgaccgc tacccaggac accagcttcc agaacggctg catcatctac aacgtcaaga
     4681 tcaacggggt gaacttccca tccaacggcc ctgtgatgca gaagaaaaca cgcggctggg
     4741 aggccaacac cgagatgctg taccccgctg acggcggcct gagaggccac agccagatgg
     4801 ccctgaagct cgtgggcggg ggctacctgc actgctcctt caagaccaca tacagatcca
     4861 agaaacccgc taagaacctc aagatgcccg gcttccactt cgtggaccac agactggaaa
     4921 gaatcaagga ggccgacaaa gaaacgtacg tcgagcagca cgagatggct gtggccaagt
     4981 actgcgacct ccctagcaaa ctggggcaca gatgaaggtg actcctgttg atagatccag
     5041 taatgacctc agaactccat ctggatttgt tcagaacgct cggttgccgc cgggcgtttt
     5101 ttattggtga gaatgcttcc aggatacata gattaccaca actccgagcc cttccacccc
     5161 aagctagctt ggaggacaaa tccgccgcta ggagcttgcg gccgccttaa gacgcgtgga
     5221 tccccgggaa tttcgacccc acgcccctct ttaatacgac gggcaatttg cacttcagaa
     5281 aatgaagagt ttgctttagc cataacaaaa gtccagtatg ctttttcaca gcataactgg
     5341 actgatttca gtttacaact attctgtcta gtttaagact ttattgtcat agtttagatc
     5401 tattttgttc agtttaagac tttattgtcc gcccacaccc gcttacgcag ggcatccatt
     5461 tattactcaa ccgtaaccga ttttgccagg ttacgcggct ggtcctctag aattcccatg
     5521 tcagccgtta agtgttcctg tgtcactcaa aattgctttg agaggctcta agggcttctc
     5581 agtgcgttac atccctggct tgttgtccac aaccgttaaa ccttaaaagc tttaaaagcc
     5641 ttatatattc ttttttttct tataaaactt aaaaccttag aggctattta agttgctgat
     5701 ttatattaat tttattgttc aaacatgaga gcttagtacg tgaaacatga gagcttagta
     5761 cgttagccat gagagcttag tacgttagcc atgagggttt agttcgttaa acatgagagc
     5821 ttagtacgtt aaacatgaga gcttagtacg tgaaacatga gagcttagta cgtactatca
     5881 acaggttgaa ctgctgatct tcagatcctc tacgccggac gcatcgtggc cggatcgggg
     5941 ggggggggcg ctcggtcttg ccttgctcgt cggtgatgta cttcaccagc tccgcgaagt
     6001 cgctcttctt gatggagcgc atggggacgt gcttggcaat cacgcgcacc ccccggccgt
     6061 tttagcggct aaaaaagtca tggctctgcc ctcgggcgga ccacgcccat catgaccttg
     6121 ccaagctcgt cctgcttctc ttcgatcttc gccagcaggg cgaggatcgt ggcatcaccg
     6181 aaccgcgccg tgcgcgggtc gtcggtgagc cagagtttca gcaggccgcc caggcggccc
     6241 aggtcgccat tgatgcgggc cagctcgcgg acgtgctcat agtccacgac gcccgtgatt
     6301 ttgtagccct ggccgacggc cagcaggtag gccgacaggc tcatgccggc cgccgccgcc
     6361 ttttcctcaa tcgctcttcg ttcgtctgga aggcagtaca ccttgatagg tgggctgccc
     6421 ttcctggttg gcttggtttc atcagccatc cgcttgccct catctgttac gccggcggta
     6481 gccggccagc ctcgcagagc aggattcccg ttgagcaccg ccaggtgcga ataagggaca
     6541 gtgaagaagg aacacccgct cgcgggtggg cctacttcac ctatcctgcc cggctgacgc
     6601 cgttggatac accaaggaaa gtctacacga accctttggc aaaatcctgt atatcgtgcg
     6661 aaaaaggatg gatataccga aaaaatcgct ataatgaccc cgaagcaggg ttatgcagcg
     6721 gaaaagcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     6781 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     6841 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     6901 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     6961 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     7021 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     7081 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
//
