LOCUS       Exported                7080 bp ds-DNA     circular SYN 06-SEP-2024
DEFINITION  Gibson/Homology Cloning ligation product.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7080)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Apr 11, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7080
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..4115
                     /label=EML002 backbone (fEL077+fEL076) extraction (con...
     misc_feature    1..4115
                     /label=Extracted region from EML002
     CDS             complement(171..1031)
                     /label=AmpR
     promoter        complement(1032..1136)
                     /label=AmpR promoter
     primer_bind     1176..1195
                     /label=oEL180
     mobile_element  complement(1210..1375)
                     /label=transposon:Tn7
     misc_feature    1475..4076
                     /label=oEL172 + oEL159 (concatenated sequence 2)
     misc_feature    1475..1504
                     /label=Ligation
     primer_bind     complement(1475..1504)
                     /label=oEL173
     primer_bind     1475..1504
                     /label=oEL172
     primer_bind     complement(1475..1504)
                     /label=oEL173
     primer_bind     1475..1504
                     /label=oEL172
     primer_bind     complement(1501..1523)
                     /label=oEL236
     CDS             1507..2322
                     /label=KanR
     primer_bind     complement(1717..1736)
                     /label=oEL179
     terminator      2701..2732
                     /label=T4-32
     primer_bind     3660..3679
                     /label=oEL178
     terminator      3756..3787
                     /label=T4-32
     promoter        complement(3891..3968)
                     /label=AmpR promoter
     primer_bind     3994..4022
                     /label=oEL245
     misc_feature    4044..4076
                     /label=Ligation
     primer_bind     complement(4049..4068)
                     /label=oEL182
     primer_bind     complement(4055..4076)
                     /label=3G_bb_R
     primer_bind     complement(4056..4076)
                     /label=oEL159
     misc_feature    4077..5108
                     /label=fOW027 (concatenated sequence 2)
     misc_feature    4077..4120
                     /label=UNS1_A (concatenated sequence 1)
     primer_bind     4077..4116
                     /label=UNS1
     misc_feature    4077..4115
                     /label=Ligation
     misc_feature    4077..4115
                     /label=UNS1
     primer_bind     4077..4115
                     /label=UNS1_F
     primer_bind     complement(4077..4115)
                     /label=UNS1_R
     misc_feature    4116
                     /label=Extracted region from fOW027
     misc_feature    4117..5068
                     /label=P2m-U25m-TurboRFP-t0 (concatenated sequence 2)
     misc_feature    4117..4159
                     /label=P2m (MAYBE) (concatenated sequence 1)
     misc_feature    4117..4159
                     /label=Editing History Insertion
     misc_feature    4117..4155
                     /label=Extracted region from P2m (MAYBE)
     misc_feature    4117..4120
                     /label=Ligation
     primer_bind     4117..4120
                     /label=Binds to A sticky end
     promoter        4121..4155
                     /label=J23119
     misc_feature    4156..4272
                     /label=U25m (concatenated sequence 2)
     misc_feature    4156..4234
                     /label=Extracted region from U25m
     misc_feature    4156..4159
                     /label=Ligation
     misc_RNA        4160..4234
                     /label=RiboJ
     misc_feature    4161..4268
                     /label=TGTCTTCTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAG...
     5'UTR           4161..4268
                     /label=U25m
     5'UTR           4161..4234
                     /label=U25m
     misc_feature    4235..4268
                     /label=Extracted region from pBEST_OR2_OR1_Pr_UTR1-deG...
     misc_feature    4235..4267
                     /label=UTR1
     RBS             4242..4262
     misc_feature    complement(4252..4268)
                     /label=ORF 9 (frame 3)
     misc_feature    4269..4969
                     /label=TurboRFP (CDS) - removed BsaI cut-site (concate...
     misc_feature    4269..4969
                     /label=TurboRFP (CDS) (concatenated sequence 2)
     misc_feature    4269..4965
                     /label=Extracted region from TurboRFP (CDS) - removed ...
     misc_feature    4269..4272
                     /label=Ligation
     misc_feature    4269
                     /label=Ligation
     misc_feature    4270..4965
                     /locus_tag="TurboRFP sequence"
                     /label=TurboRFP sequence
     primer_bind     4270..4290
                     /label=TurboRFP (CDS) (1 F) - DVLA backbone w CDS over...
     primer_bind     complement(4868..4896)
                     /label=remove_BsaI_pt2_R
     primer_bind     4893..4916
                     /label=remove_BsaI_pt1_F
     misc_feature    4893
                     /label=G
     misc_feature    4896
                     /label=C
     primer_bind     complement(4946..4965)
                     /label=TurboRFP (CDS) (696 R) - DVLA backbone w CDS ov...
     misc_feature    join(4966..4969,4269)
                     /label=DVLA backbone w CDS overhangs (concatenated seq...
     misc_feature    4966..5068
                     /label=DVLA backbone w terminator overhangs - EML001 w...
     misc_feature    4966..5068
                     /label=EML001 with UNS - lambda t0 terminator (concate...
     misc_feature    4966..4969
                     /label=Ligation
     misc_feature    4966..4969
                     /label=Ligation
     misc_feature    4966..4969
                     /label=Ligation
     misc_feature    4966..4969
                     /label=Extracted region from T2m
     terminator      complement(4970..5064)
                     /label=lambda t0 terminator
     misc_feature    4970..5064
                     /label=Extracted region from EML001 with UNS
     primer_bind     4970..4991
                     /label=EML001 with UNS - lambda t0 terminator (1 F) - ...
     primer_bind     complement(5041..5064)
                     /label=EML001 with UNS - lambda t0 terminator (95 R) -...
     misc_feature    join(5065..5068,4966..4969)
                     /label=DVLA backbone w terminator overhangs (concatena...
     misc_feature    5065..5108
                     /label=UNSX_E (concatenated sequence 3)
     misc_feature    5065..5108
                     /label=Extracted region from UNSX_E
     misc_feature    5065..5068
                     /label=Ligation
     misc_feature    5065..5068
                     /label=Ligation
     primer_bind     5065..5068
                     /label=Binds to E sticky end
     misc_feature    join(5069..5138,4044..4115)
                     /label=fEL042 (concatenated sequence 3)
     misc_feature    5069..7080
                     /label=EML002 backbone (fEL077+fEL076) extraction (con...
     misc_feature    5069..5108
                     /label=Ligation
     primer_bind     5069..5108
                     /label=UNS10
     misc_feature    5069..5108
                     /label=UNSX
     primer_bind     5069..5108
                     /label=UNSX_F
     misc_feature    5109..8584
                     /label=oEL171 + oEL173 (concatenated sequence 1)
     misc_feature    5109..5138
                     /label=Extracted region from fEL042
     misc_feature    5109..5138
                     /label=Ligation
     primer_bind     5109..5130
                     /label=oEL171
     misc_feature    5139..7080
                     /label=Extracted region from EML001 with UNS
     mobile_element  complement(5189..5387)
                     /label=transposon:Tn7
     primer_bind     complement(5396..5417)
                     /label=oEL188
     primer_bind     5397..5416
                     /label=oEL181
     rep_origin      complement(5469..5857)
                     /direction=LEFT
                     /label=R6K gamma ori
     CDS             5854..5883
                     /label=TcR
     CDS             complement(6028..6399)
                     /label=traJ
     oriT            complement(6432..6541)
                     /direction=LEFT
     rep_origin      complement(6675..7080)
                     /direction=LEFT
                     /label=ori
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc tgaattaatt cgagctcgcc caaacataac aggaagaaaa
     1201 atgccccgct gtgggcggac aaaatagttg ggaactggga ggggtggaaa tggagttttt
     1261 aaggattatt tagggaagag tgacaaaata gatgggaact gggtgtagcg tcgtaagcta
     1321 atacgaaaat taaaaatgac aaaatagttt ggaactagat ttcacttatc tggttggcct
     1381 gcaggggggg ggggggaaag ccacgttgtg tctcaaaatc tctgatgtta cattgcacaa
     1441 gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg
     1501 ggtgttatga gccatattca acgggaaacg tcttgctcga ggccgcgatt aaattccaac
     1561 atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg
     1621 acaatctatc gattgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa
     1681 ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt
     1741 atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc
     1801 actgcgatcc ccgggaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa
     1861 aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat
     1921 tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac
     1981 ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc
     2041 tggaaagaaa tgcataagct tttgccattc tcaccggatt cagtcgtcac tcatggtgat
     2101 ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga
     2161 cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag
     2221 ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg
     2281 aataaattgc agtttcattt gatgctcgat gagtttttct aatcagaatt ggttaattgg
     2341 ttgtaacact ggcagagcat tacgctgact tgacgggacg gcggctttgt tgaataaatc
     2401 gaacttttgc tgagttgaag gatcagatca cgcatcttcc cgacaacgca gaccgttccg
     2461 tggcaaagca aaagttcaaa atcaccaact ggtccaccta caacaaagct ctcatcaacc
     2521 gtggctccct cactttctgg ctggatgatg gggcgattca ggcctggtat gagtcagcaa
     2581 caccttcttc acgaggcaga cctcagcgcc cccccccccc tgcaggtcga ctagagtcgg
     2641 ggatccggtg attgattgag caagctttat gcttgtaaac cgttttgtga aaaaattttt
     2701 aaaataaaaa aggggacctc tagggtcccc aattaattag taatataatc tattaaaggt
     2761 cattcaaaag gtcatccacc ggatcaattc ccctgctcgc gcaggctggg tgccaagctc
     2821 tcgggtaaca tcaaggcccg atccttggag cccttgccct cccgcacgat gatcgtgccg
     2881 tgatcgaaat ccagatcctt gacccgcagt tgcaaaccct cactgatccg catgcccgtt
     2941 ccatacagaa gctgggcgaa caaacgatgc tcgccttcca gaaaaccgag gatgcgaacc
     3001 acttcatccg gggtcagcac caccggcaag cgccgcgacg gccgaggtct tccgatctcc
     3061 tgaagccagg gcagatccgt gcacagcacc ttgccgtaga agaacagcaa ggccgccaat
     3121 gcctgacgat gcgtggagac cgaaaccttg cgctcgttcg ccagccagga cagaaatgcc
     3181 tcgacttcgc tgctgcccaa ggttgccggg tgacgcacac cgtggaaacg gatgaaggca
     3241 cgaacccagt ggacataagc ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc
     3301 tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt
     3361 ttcatggctt gttatgactg tttttttggg gtacagtcta tgcctcgggc atccaagcag
     3421 caagcgcgtt acgccgtggg tcgatgtttg tgtctaacaa ttcgttcaag ccgacgccgc
     3481 ttcgcggcgc ggcttaactc aagcgttaga tgcactaagc acataattgc tcacagccaa
     3541 actatcaggt caagtctgct tttattattt ttaagcgtgc ataataagcc ctacacaaat
     3601 tgggagatat atcatgaaag gctggctttt tcttgttatc gcaatagttg gcgaagtaat
     3661 cgcaacatcc gcattaaaat ctagcgaggg ctttactaag ctgatccggt ggatgacctt
     3721 ttgaatgacc tttaatagat tatattacta attaattggg gaccctagag gtcccctttt
     3781 ttattttaaa aattttttca caaaacggtt tacaagcata aagcttgctc aatcaatcac
     3841 cggatcccga cgggcccggt accgcggccg cgcgaattcg agctcggtac catttatcag
     3901 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     3961 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     4021 acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcacct cgagaacatt
     4081 actcgcatcc attctcaggc tgtctcgtct cgtctcggag ttgacggcta gctcagtcct
     4141 aggtacagtg ctagctacta gctgtcaccg gatgtgcttt ccggtctgat gagtccgtga
     4201 ggacgaaaca gcctctacaa ataattttgt ttaaaataat tttgtttaac tttaagaagg
     4261 agatatacaa tgagcgagct gatcaaggag aacatgcaca tgaagctgta catggagggc
     4321 accgtgaaca accaccactt caagtgcaca tccgagggcg aaggcaagcc ctacgagggc
     4381 acccagacca tgaagatcaa ggtggtcgag ggcggccctc tccccttcgc cttcgacatc
     4441 ctggctacca gcttcatgta cggcagcaaa gccttcatca accacaccca gggcatcccc
     4501 gacttcttta agcagtcctt ccctgagggc ttcacatggg agagaatcac cacatacgaa
     4561 gacgggggcg tgctgaccgc tacccaggac accagcttcc agaacggctg catcatctac
     4621 aacgtcaaga tcaacggggt gaacttccca tccaacggcc ctgtgatgca gaagaaaaca
     4681 cgcggctggg aggccaacac cgagatgctg taccccgctg acggcggcct gagaggccac
     4741 agccagatgg ccctgaagct cgtgggcggg ggctacctgc actgctcctt caagaccaca
     4801 tacagatcca agaaacccgc taagaacctc aagatgcccg gcttccactt cgtggaccac
     4861 agactggaaa gaatcaagga ggccgacaaa gaaacgtacg tcgagcagca cgagatggct
     4921 gtggccaagt actgcgacct ccctagcaaa ctggggcaca gatgaaggtg actcctgttg
     4981 atagatccag taatgacctc agaactccat ctggatttgt tcagaacgct cggttgccgc
     5041 cgggcgtttt ttattggtga gaatgcttcc aggatacata gattaccaca actccgagcc
     5101 cttccacccc aagctagctt ggaggacaaa tccgccgcta ggagcttgcg gccgccttaa
     5161 gacgcgtgga tccccgggaa tttcgacccc acgcccctct ttaatacgac gggcaatttg
     5221 cacttcagaa aatgaagagt ttgctttagc cataacaaaa gtccagtatg ctttttcaca
     5281 gcataactgg actgatttca gtttacaact attctgtcta gtttaagact ttattgtcat
     5341 agtttagatc tattttgttc agtttaagac tttattgtcc gcccacaccc gcttacgcag
     5401 ggcatccatt tattactcaa ccgtaaccga ttttgccagg ttacgcggct ggtcctctag
     5461 aattcccatg tcagccgtta agtgttcctg tgtcactcaa aattgctttg agaggctcta
     5521 agggcttctc agtgcgttac atccctggct tgttgtccac aaccgttaaa ccttaaaagc
     5581 tttaaaagcc ttatatattc ttttttttct tataaaactt aaaaccttag aggctattta
     5641 agttgctgat ttatattaat tttattgttc aaacatgaga gcttagtacg tgaaacatga
     5701 gagcttagta cgttagccat gagagcttag tacgttagcc atgagggttt agttcgttaa
     5761 acatgagagc ttagtacgtt aaacatgaga gcttagtacg tgaaacatga gagcttagta
     5821 cgtactatca acaggttgaa ctgctgatct tcagatcctc tacgccggac gcatcgtggc
     5881 cggatcgggg ggggggggcg ctcggtcttg ccttgctcgt cggtgatgta cttcaccagc
     5941 tccgcgaagt cgctcttctt gatggagcgc atggggacgt gcttggcaat cacgcgcacc
     6001 ccccggccgt tttagcggct aaaaaagtca tggctctgcc ctcgggcgga ccacgcccat
     6061 catgaccttg ccaagctcgt cctgcttctc ttcgatcttc gccagcaggg cgaggatcgt
     6121 ggcatcaccg aaccgcgccg tgcgcgggtc gtcggtgagc cagagtttca gcaggccgcc
     6181 caggcggccc aggtcgccat tgatgcgggc cagctcgcgg acgtgctcat agtccacgac
     6241 gcccgtgatt ttgtagccct ggccgacggc cagcaggtag gccgacaggc tcatgccggc
     6301 cgccgccgcc ttttcctcaa tcgctcttcg ttcgtctgga aggcagtaca ccttgatagg
     6361 tgggctgccc ttcctggttg gcttggtttc atcagccatc cgcttgccct catctgttac
     6421 gccggcggta gccggccagc ctcgcagagc aggattcccg ttgagcaccg ccaggtgcga
     6481 ataagggaca gtgaagaagg aacacccgct cgcgggtggg cctacttcac ctatcctgcc
     6541 cggctgacgc cgttggatac accaaggaaa gtctacacga accctttggc aaaatcctgt
     6601 atatcgtgcg aaaaaggatg gatataccga aaaaatcgct ataatgaccc cgaagcaggg
     6661 ttatgcagcg gaaaagcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     6721 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     6781 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     6841 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     6901 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     6961 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     7021 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
//
