LOCUS       Exported                8323 bp ds-DNA     circular SYN 20-NOV-2024
DEFINITION  Generated by Gibson/Homology Cloning (EML002 backbone 
            (fEL077+fEL076), P4d-U3d-C63m (lacI)-t12m, P61m 
            (Ptac)-U3d-TurboRFP-t0).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8323)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Apr 11, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8323
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..4115
                     /label=EML002 backbone (fEL077+fEL076) extraction (con...
     misc_feature    1..4115
                     /label=Extracted region from EML002
     CDS             complement(171..1031)
                     /label=AmpR
     promoter        complement(1032..1136)
                     /label=AmpR promoter
     primer_bind     1176..1195
                     /label=oEL180
     mobile_element  complement(1210..1375)
                     /label=transposon:Tn7
     misc_feature    1475..4076
                     /label=oEL172 + oEL159 (concatenated sequence 2)
     misc_feature    1475..1504
                     /label=Ligation
     primer_bind     complement(1475..1504)
                     /label=oEL173
     primer_bind     1475..1504
                     /label=oEL172
     primer_bind     complement(1475..1504)
                     /label=oEL173
     primer_bind     1475..1504
                     /label=oEL172
     primer_bind     complement(1501..1523)
                     /label=oEL236
     CDS             1507..2322
                     /label=KanR
     primer_bind     complement(1717..1736)
                     /label=oEL179
     terminator      2701..2732
                     /label=T4-32
     primer_bind     3660..3679
                     /label=oEL178
     terminator      3756..3787
                     /label=T4-32
     promoter        complement(3891..3968)
                     /label=AmpR promoter
     primer_bind     3994..4022
                     /label=oEL245
     misc_feature    4044..4076
                     /label=Ligation
     primer_bind     complement(4049..4068)
                     /label=oEL182
     primer_bind     complement(4055..4076)
                     /label=3G_bb_R
     primer_bind     complement(4056..4076)
                     /label=oEL159
     misc_feature    4077..5411
                     /label=P4d-U3d-C63m (lacI)-t12m (concatenated sequence 2)
     misc_feature    4077..4120
                     /label=UNS1_A (concatenated sequence 1)
     primer_bind     4077..4116
                     /label=UNS1
     misc_feature    4077..4115
                     /label=Ligation
     misc_feature    4077..4115
                     /label=UNS1
     primer_bind     4077..4115
                     /label=UNS1_F
     primer_bind     complement(4077..4115)
                     /label=UNS1_R
     misc_feature    4116
                     /label=Extracted region from P4d-U3d-C63m (lacI)-t12m
     misc_feature    4117..4159
                     /label=P4d (concatenated sequence 2)
     misc_feature    4117..4155
                     /label=Extracted region from P4d
     misc_feature    4117..4120
                     /label=Ligation
     primer_bind     4117..4120
                     /label=Binds to A sticky end
     promoter        4121..4155
                     /label=CIDAR medium promoter J23106 (1185x); J23106 Pr...
     misc_feature    4156..4184
                     /label=U3d (concatenated sequence 3)
     misc_feature    4156..4180
                     /label=Extracted region from U3d
     misc_feature    4156..4159
                     /label=Ligation
     RBS             4160..4180
                     /label=B0034m
     5'UTR           4164..4175
                     /label=B0034
     misc_feature    4181..5277
                     /label=C63m (concatenated sequence 4)
     misc_feature    4181..5273
                     /label=Extracted region from C63m
     misc_feature    4181..4184
                     /label=Ligation
     misc_feature    4181
                     /label=Editing History Insertion
     CDS             4182..5273
                     /label=lacI (no BbsI sites, no tags)
     primer_bind     4182..4211
                     /label=bbsAATG_lacI_beg_F
     misc_feature    complement(5254..5270)
                     /label=bbsAGGT_lacI_end_R
     misc_feature    5274..5371
                     /label=T12m (concatenated sequence 5)
     misc_feature    5274..5367
                     /label=Extracted region from T12m
     misc_feature    5274..5277
                     /label=Ligation
     terminator      5278..5367
                     /label=ECK120029600 (strongest natural Voigt, 378x)
     misc_feature    5368..5411
                     /label=UNS3_E (concatenated sequence 6)
     misc_feature    5368..5411
                     /label=Extracted region from UNS3_E
     misc_feature    5368..5371
                     /label=Ligation
     misc_feature    5368..5371
                     /label=Editing History Insertion
     misc_feature    5372..6351
                     /label=P61m (Ptac)-U3d-TurboRFP-t0 (concatenated seque...
     misc_feature    5372..5415
                     /label=UNS3_A (concatenated sequence 1)
     misc_feature    5372..5411
                     /label=Ligation
     CDS             5372..5411
                     /label=UNS 3
     misc_feature    5412..5490
                     /label=p61m (concatenated sequence 2)
     misc_feature    5412..5486
                     /label=Extracted region from p61m
     misc_feature    5412..5415
                     /label=Ligation
     misc_feature    5412..5415
                     /label=Editing History Insertion
     primer_bind     5416..5430
                     /label=oAJM.093
     primer_bind     5416..5430
                     /label=oAJM.186
     misc_feature    5416..5430
                     /label=Pre-Ptac random DNA
     promoter        5431..5486
                     /label=pTac Minimal Promoter
     promoter        5433..5461
                     /label=tac promoter
     protein_bind    5467..5486
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
     misc_feature    5487..5515
                     /label=U3d (concatenated sequence 3)
     misc_feature    5487..5511
                     /label=Extracted region from U3d
     misc_feature    5487..5490
                     /label=Ligation
     RBS             5491..5511
                     /label=B0034m
     5'UTR           5495..5506
                     /label=B0034
     misc_feature    5512..6212
                     /label=TurboRFP (CDS) - removed BsaI cut-site (concate...
     misc_feature    5512..6212
                     /label=TurboRFP (CDS) (concatenated sequence 2)
     misc_feature    5512..6208
                     /label=Extracted region from TurboRFP (CDS) - removed ...
     misc_feature    5512..5515
                     /label=Ligation
     misc_feature    5512
                     /label=Ligation
     misc_feature    5513..6208
                     /locus_tag="TurboRFP sequence"
                     /label=TurboRFP sequence
     primer_bind     5513..5533
                     /label=TurboRFP (CDS) (1 F) - DVLA backbone w CDS over...
     primer_bind     complement(6111..6139)
                     /label=remove_BsaI_pt2_R
     primer_bind     6136..6159
                     /label=remove_BsaI_pt1_F
     misc_feature    6136
                     /label=G
     misc_feature    6139
                     /label=C
     primer_bind     complement(6189..6208)
                     /label=TurboRFP (CDS) (696 R) - DVLA backbone w CDS ov...
     misc_feature    join(6209..6212,5512)
                     /label=DVLA backbone w CDS overhangs (concatenated seq...
     misc_feature    6209..6311
                     /label=DVLA backbone w terminator overhangs - EML001 w...
     misc_feature    6209..6311
                     /label=EML001 with UNS - lambda t0 terminator (concate...
     misc_feature    6209..6212
                     /label=Ligation
     misc_feature    6209..6212
                     /label=Ligation
     misc_feature    6209..6212
                     /label=Ligation
     misc_feature    6209..6212
                     /label=Extracted region from T2m
     terminator      complement(6213..6307)
                     /label=lambda t0 terminator
     misc_feature    6213..6307
                     /label=Extracted region from EML001 with UNS
     primer_bind     6213..6234
                     /label=EML001 with UNS - lambda t0 terminator (1 F) - ...
     primer_bind     complement(6284..6307)
                     /label=EML001 with UNS - lambda t0 terminator (95 R) -...
     misc_feature    join(6308..6311,6209..6212)
                     /label=DVLA backbone w terminator overhangs (concatena...
     misc_feature    6308..6351
                     /label=UNSX_E (concatenated sequence 6)
     misc_feature    6308..6351
                     /label=Extracted region from UNSX_E
     misc_feature    6308..6311
                     /label=Ligation
     misc_feature    6308..6311
                     /label=Ligation
     primer_bind     6308..6311
                     /label=Binds to E sticky end
     misc_feature    join(6312..6381,4044..4115)
                     /label=fEL042 (concatenated sequence 3)
     misc_feature    6312..8323
                     /label=EML002 backbone (fEL077+fEL076) extraction (con...
     misc_feature    6312..6351
                     /label=Ligation
     primer_bind     6312..6351
                     /label=UNS10
     misc_feature    6312..6351
                     /label=UNSX
     primer_bind     6312..6351
                     /label=UNSX_F
     misc_feature    6352..9827
                     /label=oEL171 + oEL173 (concatenated sequence 1)
     misc_feature    6352..6381
                     /label=Extracted region from fEL042
     misc_feature    6352..6381
                     /label=Ligation
     primer_bind     6352..6373
                     /label=oEL171
     misc_feature    6382..8323
                     /label=Extracted region from EML001 with UNS
     mobile_element  complement(6432..6630)
                     /label=transposon:Tn7
     primer_bind     complement(6639..6660)
                     /label=oEL188
     primer_bind     6640..6659
                     /label=oEL181
     rep_origin      complement(6712..7100)
                     /direction=LEFT
                     /label=R6K gamma ori
     CDS             7097..7126
                     /label=TcR
     CDS             complement(7271..7642)
                     /label=traJ
     oriT            complement(7675..7784)
                     /direction=LEFT
     rep_origin      complement(7918..8323)
                     /direction=LEFT
                     /label=ori
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc tgaattaatt cgagctcgcc caaacataac aggaagaaaa
     1201 atgccccgct gtgggcggac aaaatagttg ggaactggga ggggtggaaa tggagttttt
     1261 aaggattatt tagggaagag tgacaaaata gatgggaact gggtgtagcg tcgtaagcta
     1321 atacgaaaat taaaaatgac aaaatagttt ggaactagat ttcacttatc tggttggcct
     1381 gcaggggggg ggggggaaag ccacgttgtg tctcaaaatc tctgatgtta cattgcacaa
     1441 gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg
     1501 ggtgttatga gccatattca acgggaaacg tcttgctcga ggccgcgatt aaattccaac
     1561 atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg
     1621 acaatctatc gattgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa
     1681 ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt
     1741 atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc
     1801 actgcgatcc ccgggaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa
     1861 aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat
     1921 tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac
     1981 ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc
     2041 tggaaagaaa tgcataagct tttgccattc tcaccggatt cagtcgtcac tcatggtgat
     2101 ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga
     2161 cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag
     2221 ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg
     2281 aataaattgc agtttcattt gatgctcgat gagtttttct aatcagaatt ggttaattgg
     2341 ttgtaacact ggcagagcat tacgctgact tgacgggacg gcggctttgt tgaataaatc
     2401 gaacttttgc tgagttgaag gatcagatca cgcatcttcc cgacaacgca gaccgttccg
     2461 tggcaaagca aaagttcaaa atcaccaact ggtccaccta caacaaagct ctcatcaacc
     2521 gtggctccct cactttctgg ctggatgatg gggcgattca ggcctggtat gagtcagcaa
     2581 caccttcttc acgaggcaga cctcagcgcc cccccccccc tgcaggtcga ctagagtcgg
     2641 ggatccggtg attgattgag caagctttat gcttgtaaac cgttttgtga aaaaattttt
     2701 aaaataaaaa aggggacctc tagggtcccc aattaattag taatataatc tattaaaggt
     2761 cattcaaaag gtcatccacc ggatcaattc ccctgctcgc gcaggctggg tgccaagctc
     2821 tcgggtaaca tcaaggcccg atccttggag cccttgccct cccgcacgat gatcgtgccg
     2881 tgatcgaaat ccagatcctt gacccgcagt tgcaaaccct cactgatccg catgcccgtt
     2941 ccatacagaa gctgggcgaa caaacgatgc tcgccttcca gaaaaccgag gatgcgaacc
     3001 acttcatccg gggtcagcac caccggcaag cgccgcgacg gccgaggtct tccgatctcc
     3061 tgaagccagg gcagatccgt gcacagcacc ttgccgtaga agaacagcaa ggccgccaat
     3121 gcctgacgat gcgtggagac cgaaaccttg cgctcgttcg ccagccagga cagaaatgcc
     3181 tcgacttcgc tgctgcccaa ggttgccggg tgacgcacac cgtggaaacg gatgaaggca
     3241 cgaacccagt ggacataagc ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc
     3301 tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt
     3361 ttcatggctt gttatgactg tttttttggg gtacagtcta tgcctcgggc atccaagcag
     3421 caagcgcgtt acgccgtggg tcgatgtttg tgtctaacaa ttcgttcaag ccgacgccgc
     3481 ttcgcggcgc ggcttaactc aagcgttaga tgcactaagc acataattgc tcacagccaa
     3541 actatcaggt caagtctgct tttattattt ttaagcgtgc ataataagcc ctacacaaat
     3601 tgggagatat atcatgaaag gctggctttt tcttgttatc gcaatagttg gcgaagtaat
     3661 cgcaacatcc gcattaaaat ctagcgaggg ctttactaag ctgatccggt ggatgacctt
     3721 ttgaatgacc tttaatagat tatattacta attaattggg gaccctagag gtcccctttt
     3781 ttattttaaa aattttttca caaaacggtt tacaagcata aagcttgctc aatcaatcac
     3841 cggatcccga cgggcccggt accgcggccg cgcgaattcg agctcggtac catttatcag
     3901 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     3961 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     4021 acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcacct cgagaacatt
     4081 actcgcatcc attctcaggc tgtctcgtct cgtctcggag tttacggcta gctcagtcct
     4141 aggtatagtg ctagctacta gagaaagagg agaaatacta aatggtgaat gtgaaaccag
     4201 taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg
     4261 tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg
     4321 agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga
     4381 ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta
     4441 aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg
     4501 tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca
     4561 ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc actaatgttc
     4621 cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg
     4681 aggacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc
     4741 tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat
     4801 atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg agtgccatgt
     4861 ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact gcgatgctgg
     4921 ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg
     4981 ttggtgcgga tatctcggta gtgggatacg acgataccga agatagctca tgttatatcc
     5041 cgccgttaac caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct
     5101 tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg
     5161 tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     5221 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag taaaggtttc
     5281 agccaaaaaa cttaagaccg ccggtcttgt ccactacctt gcagtaatgc ggtggacagg
     5341 atcggcggtt ttcttttctc ttctcaagct tgcactgaag gtcctcaatc gcactggaaa
     5401 catcaaggtc gggagaacga tcgttggctg tgttgacaat taatcatcgg ctcgtataat
     5461 gtgtggaatt gtgagcgctc acaatttact agagaaagag gagaaatact aaatgagcga
     5521 gctgatcaag gagaacatgc acatgaagct gtacatggag ggcaccgtga acaaccacca
     5581 cttcaagtgc acatccgagg gcgaaggcaa gccctacgag ggcacccaga ccatgaagat
     5641 caaggtggtc gagggcggcc ctctcccctt cgccttcgac atcctggcta ccagcttcat
     5701 gtacggcagc aaagccttca tcaaccacac ccagggcatc cccgacttct ttaagcagtc
     5761 cttccctgag ggcttcacat gggagagaat caccacatac gaagacgggg gcgtgctgac
     5821 cgctacccag gacaccagct tccagaacgg ctgcatcatc tacaacgtca agatcaacgg
     5881 ggtgaacttc ccatccaacg gccctgtgat gcagaagaaa acacgcggct gggaggccaa
     5941 caccgagatg ctgtaccccg ctgacggcgg cctgagaggc cacagccaga tggccctgaa
     6001 gctcgtgggc gggggctacc tgcactgctc cttcaagacc acatacagat ccaagaaacc
     6061 cgctaagaac ctcaagatgc ccggcttcca cttcgtggac cacagactgg aaagaatcaa
     6121 ggaggccgac aaagaaacgt acgtcgagca gcacgagatg gctgtggcca agtactgcga
     6181 cctccctagc aaactggggc acagatgaag gtgactcctg ttgatagatc cagtaatgac
     6241 ctcagaactc catctggatt tgttcagaac gctcggttgc cgccgggcgt tttttattgg
     6301 tgagaatgct tccaggatac atagattacc acaactccga gcccttccac cccaagctag
     6361 cttggaggac aaatccgccg ctaggagctt gcggccgcct taagacgcgt ggatccccgg
     6421 gaatttcgac cccacgcccc tctttaatac gacgggcaat ttgcacttca gaaaatgaag
     6481 agtttgcttt agccataaca aaagtccagt atgctttttc acagcataac tggactgatt
     6541 tcagtttaca actattctgt ctagtttaag actttattgt catagtttag atctattttg
     6601 ttcagtttaa gactttattg tccgcccaca cccgcttacg cagggcatcc atttattact
     6661 caaccgtaac cgattttgcc aggttacgcg gctggtcctc tagaattccc atgtcagccg
     6721 ttaagtgttc ctgtgtcact caaaattgct ttgagaggct ctaagggctt ctcagtgcgt
     6781 tacatccctg gcttgttgtc cacaaccgtt aaaccttaaa agctttaaaa gccttatata
     6841 ttcttttttt tcttataaaa cttaaaacct tagaggctat ttaagttgct gatttatatt
     6901 aattttattg ttcaaacatg agagcttagt acgtgaaaca tgagagctta gtacgttagc
     6961 catgagagct tagtacgtta gccatgaggg tttagttcgt taaacatgag agcttagtac
     7021 gttaaacatg agagcttagt acgtgaaaca tgagagctta gtacgtacta tcaacaggtt
     7081 gaactgctga tcttcagatc ctctacgccg gacgcatcgt ggccggatcg gggggggggg
     7141 gcgctcggtc ttgccttgct cgtcggtgat gtacttcacc agctccgcga agtcgctctt
     7201 cttgatggag cgcatgggga cgtgcttggc aatcacgcgc accccccggc cgttttagcg
     7261 gctaaaaaag tcatggctct gccctcgggc ggaccacgcc catcatgacc ttgccaagct
     7321 cgtcctgctt ctcttcgatc ttcgccagca gggcgaggat cgtggcatca ccgaaccgcg
     7381 ccgtgcgcgg gtcgtcggtg agccagagtt tcagcaggcc gcccaggcgg cccaggtcgc
     7441 cattgatgcg ggccagctcg cggacgtgct catagtccac gacgcccgtg attttgtagc
     7501 cctggccgac ggccagcagg taggccgaca ggctcatgcc ggccgccgcc gccttttcct
     7561 caatcgctct tcgttcgtct ggaaggcagt acaccttgat aggtgggctg cccttcctgg
     7621 ttggcttggt ttcatcagcc atccgcttgc cctcatctgt tacgccggcg gtagccggcc
     7681 agcctcgcag agcaggattc ccgttgagca ccgccaggtg cgaataaggg acagtgaaga
     7741 aggaacaccc gctcgcgggt gggcctactt cacctatcct gcccggctga cgccgttgga
     7801 tacaccaagg aaagtctaca cgaacccttt ggcaaaatcc tgtatatcgt gcgaaaaagg
     7861 atggatatac cgaaaaaatc gctataatga ccccgaagca gggttatgca gcggaaaagc
     7921 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     7981 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     8041 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     8101 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     8161 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     8221 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     8281 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caa
//
