LOCUS       Exported                8324 bp ds-DNA     circular SYN 20-NOV-2024
DEFINITION  Generated by Gibson/Homology Cloning (EML002 backbone 
            (fEL077+fEL076), P4d-U1d-C63m (lacI)-t12m, P61m 
            (Ptac)-U3d-TurboRFP-t0).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8324)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Apr 11, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8324
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..4115
                     /label=EML002 backbone (fEL077+fEL076) extraction (con...
     misc_feature    1..4115
                     /label=Extracted region from EML002
     CDS             complement(171..1031)
                     /label=AmpR
     promoter        complement(1032..1136)
                     /label=AmpR promoter
     primer_bind     1176..1195
                     /label=oEL180
     mobile_element  complement(1210..1375)
                     /label=transposon:Tn7
     misc_feature    1475..4076
                     /label=oEL172 + oEL159 (concatenated sequence 2)
     misc_feature    1475..1504
                     /label=Ligation
     primer_bind     complement(1475..1504)
                     /label=oEL173
     primer_bind     1475..1504
                     /label=oEL172
     primer_bind     complement(1475..1504)
                     /label=oEL173
     primer_bind     1475..1504
                     /label=oEL172
     primer_bind     complement(1501..1523)
                     /label=oEL236
     CDS             1507..2322
                     /label=KanR
     primer_bind     complement(1717..1736)
                     /label=oEL179
     terminator      2701..2732
                     /label=T4-32
     primer_bind     3660..3679
                     /label=oEL178
     terminator      3756..3787
                     /label=T4-32
     promoter        complement(3891..3968)
                     /label=AmpR promoter
     primer_bind     3994..4022
                     /label=oEL245
     misc_feature    4044..4076
                     /label=Ligation
     primer_bind     complement(4049..4068)
                     /label=oEL182
     primer_bind     complement(4055..4076)
                     /label=3G_bb_R
     primer_bind     complement(4056..4076)
                     /label=oEL159
     misc_feature    4077..5412
                     /label=P4d-U1d-C63m (lacI)-t12m (concatenated sequence 2)
     misc_feature    4077..4120
                     /label=UNS1_A (concatenated sequence 1)
     primer_bind     4077..4116
                     /label=UNS1
     misc_feature    4077..4115
                     /label=Ligation
     misc_feature    4077..4115
                     /label=UNS1
     primer_bind     4077..4115
                     /label=UNS1_F
     primer_bind     complement(4077..4115)
                     /label=UNS1_R
     misc_feature    4116
                     /label=Extracted region from P4d-U1d-C63m (lacI)-t12m
     misc_feature    4117..4159
                     /label=P4d (concatenated sequence 2)
     misc_feature    4117..4155
                     /label=Extracted region from P4d
     misc_feature    4117..4120
                     /label=Ligation
     primer_bind     4117..4120
                     /label=Binds to A sticky end
     promoter        4121..4155
                     /label=CIDAR medium promoter J23106 (1185x); J23106 Pr...
     misc_feature    4156..4185
                     /label=U1d (concatenated sequence 3)
     misc_feature    4156..4159
                     /label=Ligation
     misc_feature    4156..4159
                     /label=Extracted region from U1d
     misc_feature    join(4160..8324,1..4159)
                     /label=origin
     RBS             4160..4181
                     /label=B0032 0.3x RBS
     misc_feature    4160..4181
                     /label=Extracted region from U1d
     misc_feature    4182..5278
                     /label=C63m (concatenated sequence 4)
     misc_feature    4182..5274
                     /label=Extracted region from C63m
     misc_feature    4182..4185
                     /label=Ligation
     misc_feature    4182
                     /label=Editing History Insertion
     CDS             4183..5274
                     /label=lacI (no BbsI sites, no tags)
     primer_bind     4183..4212
                     /label=bbsAATG_lacI_beg_F
     misc_feature    complement(5255..5271)
                     /label=bbsAGGT_lacI_end_R
     misc_feature    5275..5372
                     /label=T12m (concatenated sequence 5)
     misc_feature    5275..5368
                     /label=Extracted region from T12m
     misc_feature    5275..5278
                     /label=Ligation
     terminator      5279..5368
                     /label=ECK120029600 (strongest natural Voigt, 378x)
     misc_feature    5369..5412
                     /label=UNS3_E (concatenated sequence 6)
     misc_feature    5369..5412
                     /label=Extracted region from UNS3_E
     misc_feature    5369..5372
                     /label=Ligation
     misc_feature    5369..5372
                     /label=Editing History Insertion
     misc_feature    5373..6352
                     /label=P61m (Ptac)-U3d-TurboRFP-t0 (concatenated seque...
     misc_feature    5373..5416
                     /label=UNS3_A (concatenated sequence 1)
     misc_feature    5373..5412
                     /label=Ligation
     CDS             5373..5412
                     /label=UNS 3
     misc_feature    5413..5491
                     /label=p61m (concatenated sequence 2)
     misc_feature    5413..5487
                     /label=Extracted region from p61m
     misc_feature    5413..5416
                     /label=Ligation
     misc_feature    5413..5416
                     /label=Editing History Insertion
     primer_bind     5417..5431
                     /label=oAJM.093
     primer_bind     5417..5431
                     /label=oAJM.186
     misc_feature    5417..5431
                     /label=Pre-Ptac random DNA
     promoter        5432..5487
                     /label=pTac Minimal Promoter
     promoter        5434..5462
                     /label=tac promoter
     protein_bind    5468..5487
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
     misc_feature    5488..5516
                     /label=U3d (concatenated sequence 3)
     misc_feature    5488..5512
                     /label=Extracted region from U3d
     misc_feature    5488..5491
                     /label=Ligation
     RBS             5492..5512
                     /label=B0034m
     5'UTR           5496..5507
                     /label=B0034
     misc_feature    5513..6213
                     /label=TurboRFP (CDS) - removed BsaI cut-site (concate...
     misc_feature    5513..6213
                     /label=TurboRFP (CDS) (concatenated sequence 2)
     misc_feature    5513..6209
                     /label=Extracted region from TurboRFP (CDS) - removed ...
     misc_feature    5513..5516
                     /label=Ligation
     misc_feature    5513
                     /label=Ligation
     misc_feature    5514..6209
                     /locus_tag="TurboRFP sequence"
                     /label=TurboRFP sequence
     primer_bind     5514..5534
                     /label=TurboRFP (CDS) (1 F) - DVLA backbone w CDS over...
     primer_bind     complement(6112..6140)
                     /label=remove_BsaI_pt2_R
     primer_bind     6137..6160
                     /label=remove_BsaI_pt1_F
     misc_feature    6137
                     /label=G
     misc_feature    6140
                     /label=C
     primer_bind     complement(6190..6209)
                     /label=TurboRFP (CDS) (696 R) - DVLA backbone w CDS ov...
     misc_feature    join(6210..6213,5513)
                     /label=DVLA backbone w CDS overhangs (concatenated seq...
     misc_feature    6210..6312
                     /label=DVLA backbone w terminator overhangs - EML001 w...
     misc_feature    6210..6312
                     /label=EML001 with UNS - lambda t0 terminator (concate...
     misc_feature    6210..6213
                     /label=Ligation
     misc_feature    6210..6213
                     /label=Ligation
     misc_feature    6210..6213
                     /label=Ligation
     misc_feature    6210..6213
                     /label=Extracted region from T2m
     terminator      complement(6214..6308)
                     /label=lambda t0 terminator
     misc_feature    6214..6308
                     /label=Extracted region from EML001 with UNS
     primer_bind     6214..6235
                     /label=EML001 with UNS - lambda t0 terminator (1 F) - ...
     primer_bind     complement(6285..6308)
                     /label=EML001 with UNS - lambda t0 terminator (95 R) -...
     misc_feature    join(6309..6312,6210..6213)
                     /label=DVLA backbone w terminator overhangs (concatena...
     misc_feature    6309..6352
                     /label=UNSX_E (concatenated sequence 6)
     misc_feature    6309..6352
                     /label=Extracted region from UNSX_E
     misc_feature    6309..6312
                     /label=Ligation
     misc_feature    6309..6312
                     /label=Ligation
     primer_bind     6309..6312
                     /label=Binds to E sticky end
     misc_feature    join(6313..6382,4044..4115)
                     /label=fEL042 (concatenated sequence 3)
     misc_feature    6313..8324
                     /label=EML002 backbone (fEL077+fEL076) extraction (con...
     misc_feature    6313..6352
                     /label=Ligation
     primer_bind     6313..6352
                     /label=UNS10
     misc_feature    6313..6352
                     /label=UNSX
     primer_bind     6313..6352
                     /label=UNSX_F
     misc_feature    6353..9828
                     /label=oEL171 + oEL173 (concatenated sequence 1)
     misc_feature    6353..6382
                     /label=Extracted region from fEL042
     misc_feature    6353..6382
                     /label=Ligation
     primer_bind     6353..6374
                     /label=oEL171
     misc_feature    6383..8324
                     /label=Extracted region from EML001 with UNS
     mobile_element  complement(6433..6631)
                     /label=transposon:Tn7
     primer_bind     complement(6640..6661)
                     /label=oEL188
     primer_bind     6641..6660
                     /label=oEL181
     rep_origin      complement(6713..7101)
                     /direction=LEFT
                     /label=R6K gamma ori
     CDS             7098..7127
                     /label=TcR
     CDS             complement(7272..7643)
                     /label=traJ
     oriT            complement(7676..7785)
                     /direction=LEFT
     rep_origin      complement(7919..8324)
                     /direction=LEFT
                     /label=ori
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc tgaattaatt cgagctcgcc caaacataac aggaagaaaa
     1201 atgccccgct gtgggcggac aaaatagttg ggaactggga ggggtggaaa tggagttttt
     1261 aaggattatt tagggaagag tgacaaaata gatgggaact gggtgtagcg tcgtaagcta
     1321 atacgaaaat taaaaatgac aaaatagttt ggaactagat ttcacttatc tggttggcct
     1381 gcaggggggg ggggggaaag ccacgttgtg tctcaaaatc tctgatgtta cattgcacaa
     1441 gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg
     1501 ggtgttatga gccatattca acgggaaacg tcttgctcga ggccgcgatt aaattccaac
     1561 atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg
     1621 acaatctatc gattgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa
     1681 ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt
     1741 atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc
     1801 actgcgatcc ccgggaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa
     1861 aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat
     1921 tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac
     1981 ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc
     2041 tggaaagaaa tgcataagct tttgccattc tcaccggatt cagtcgtcac tcatggtgat
     2101 ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga
     2161 cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag
     2221 ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg
     2281 aataaattgc agtttcattt gatgctcgat gagtttttct aatcagaatt ggttaattgg
     2341 ttgtaacact ggcagagcat tacgctgact tgacgggacg gcggctttgt tgaataaatc
     2401 gaacttttgc tgagttgaag gatcagatca cgcatcttcc cgacaacgca gaccgttccg
     2461 tggcaaagca aaagttcaaa atcaccaact ggtccaccta caacaaagct ctcatcaacc
     2521 gtggctccct cactttctgg ctggatgatg gggcgattca ggcctggtat gagtcagcaa
     2581 caccttcttc acgaggcaga cctcagcgcc cccccccccc tgcaggtcga ctagagtcgg
     2641 ggatccggtg attgattgag caagctttat gcttgtaaac cgttttgtga aaaaattttt
     2701 aaaataaaaa aggggacctc tagggtcccc aattaattag taatataatc tattaaaggt
     2761 cattcaaaag gtcatccacc ggatcaattc ccctgctcgc gcaggctggg tgccaagctc
     2821 tcgggtaaca tcaaggcccg atccttggag cccttgccct cccgcacgat gatcgtgccg
     2881 tgatcgaaat ccagatcctt gacccgcagt tgcaaaccct cactgatccg catgcccgtt
     2941 ccatacagaa gctgggcgaa caaacgatgc tcgccttcca gaaaaccgag gatgcgaacc
     3001 acttcatccg gggtcagcac caccggcaag cgccgcgacg gccgaggtct tccgatctcc
     3061 tgaagccagg gcagatccgt gcacagcacc ttgccgtaga agaacagcaa ggccgccaat
     3121 gcctgacgat gcgtggagac cgaaaccttg cgctcgttcg ccagccagga cagaaatgcc
     3181 tcgacttcgc tgctgcccaa ggttgccggg tgacgcacac cgtggaaacg gatgaaggca
     3241 cgaacccagt ggacataagc ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc
     3301 tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt
     3361 ttcatggctt gttatgactg tttttttggg gtacagtcta tgcctcgggc atccaagcag
     3421 caagcgcgtt acgccgtggg tcgatgtttg tgtctaacaa ttcgttcaag ccgacgccgc
     3481 ttcgcggcgc ggcttaactc aagcgttaga tgcactaagc acataattgc tcacagccaa
     3541 actatcaggt caagtctgct tttattattt ttaagcgtgc ataataagcc ctacacaaat
     3601 tgggagatat atcatgaaag gctggctttt tcttgttatc gcaatagttg gcgaagtaat
     3661 cgcaacatcc gcattaaaat ctagcgaggg ctttactaag ctgatccggt ggatgacctt
     3721 ttgaatgacc tttaatagat tatattacta attaattggg gaccctagag gtcccctttt
     3781 ttattttaaa aattttttca caaaacggtt tacaagcata aagcttgctc aatcaatcac
     3841 cggatcccga cgggcccggt accgcggccg cgcgaattcg agctcggtac catttatcag
     3901 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     3961 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     4021 acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcacct cgagaacatt
     4081 actcgcatcc attctcaggc tgtctcgtct cgtctcggag tttacggcta gctcagtcct
     4141 aggtatagtg ctagctacta gagtcacaca ggaaagtact aaatggtgaa tgtgaaacca
     4201 gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg
     4261 gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg
     4321 gagctgaatt acattcccaa ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg
     4381 attggcgttg ccacctccag tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt
     4441 aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt cgatggtaga acgaagcggc
     4501 gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc
     4561 attaactatc cgctggatga ccaggatgcc attgctgtgg aagctgcctg cactaatgtt
     4621 ccggcgttat ttcttgatgt ctctgaccag acacccatca acagtattat tttctcccat
     4681 gaggacggta cgcgactggg cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg
     4741 ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa
     4801 tatctcactc gcaatcaaat tcagccgata gcggaacggg aaggcgactg gagtgccatg
     4861 tccggttttc aacaaaccat gcaaatgctg aatgagggca tcgttcccac tgcgatgctg
     4921 gttgccaacg atcagatggc gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc
     4981 gttggtgcgg atatctcggt agtgggatac gacgataccg aagatagctc atgttatatc
     5041 ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc
     5101 ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc agctgttgcc cgtctcactg
     5161 gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     5221 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtaaaggttt
     5281 cagccaaaaa acttaagacc gccggtcttg tccactacct tgcagtaatg cggtggacag
     5341 gatcggcggt tttcttttct cttctcaagc ttgcactgaa ggtcctcaat cgcactggaa
     5401 acatcaaggt cgggagaacg atcgttggct gtgttgacaa ttaatcatcg gctcgtataa
     5461 tgtgtggaat tgtgagcgct cacaatttac tagagaaaga ggagaaatac taaatgagcg
     5521 agctgatcaa ggagaacatg cacatgaagc tgtacatgga gggcaccgtg aacaaccacc
     5581 acttcaagtg cacatccgag ggcgaaggca agccctacga gggcacccag accatgaaga
     5641 tcaaggtggt cgagggcggc cctctcccct tcgccttcga catcctggct accagcttca
     5701 tgtacggcag caaagccttc atcaaccaca cccagggcat ccccgacttc tttaagcagt
     5761 ccttccctga gggcttcaca tgggagagaa tcaccacata cgaagacggg ggcgtgctga
     5821 ccgctaccca ggacaccagc ttccagaacg gctgcatcat ctacaacgtc aagatcaacg
     5881 gggtgaactt cccatccaac ggccctgtga tgcagaagaa aacacgcggc tgggaggcca
     5941 acaccgagat gctgtacccc gctgacggcg gcctgagagg ccacagccag atggccctga
     6001 agctcgtggg cgggggctac ctgcactgct ccttcaagac cacatacaga tccaagaaac
     6061 ccgctaagaa cctcaagatg cccggcttcc acttcgtgga ccacagactg gaaagaatca
     6121 aggaggccga caaagaaacg tacgtcgagc agcacgagat ggctgtggcc aagtactgcg
     6181 acctccctag caaactgggg cacagatgaa ggtgactcct gttgatagat ccagtaatga
     6241 cctcagaact ccatctggat ttgttcagaa cgctcggttg ccgccgggcg ttttttattg
     6301 gtgagaatgc ttccaggata catagattac cacaactccg agcccttcca ccccaagcta
     6361 gcttggagga caaatccgcc gctaggagct tgcggccgcc ttaagacgcg tggatccccg
     6421 ggaatttcga ccccacgccc ctctttaata cgacgggcaa tttgcacttc agaaaatgaa
     6481 gagtttgctt tagccataac aaaagtccag tatgcttttt cacagcataa ctggactgat
     6541 ttcagtttac aactattctg tctagtttaa gactttattg tcatagttta gatctatttt
     6601 gttcagttta agactttatt gtccgcccac acccgcttac gcagggcatc catttattac
     6661 tcaaccgtaa ccgattttgc caggttacgc ggctggtcct ctagaattcc catgtcagcc
     6721 gttaagtgtt cctgtgtcac tcaaaattgc tttgagaggc tctaagggct tctcagtgcg
     6781 ttacatccct ggcttgttgt ccacaaccgt taaaccttaa aagctttaaa agccttatat
     6841 attctttttt ttcttataaa acttaaaacc ttagaggcta tttaagttgc tgatttatat
     6901 taattttatt gttcaaacat gagagcttag tacgtgaaac atgagagctt agtacgttag
     6961 ccatgagagc ttagtacgtt agccatgagg gtttagttcg ttaaacatga gagcttagta
     7021 cgttaaacat gagagcttag tacgtgaaac atgagagctt agtacgtact atcaacaggt
     7081 tgaactgctg atcttcagat cctctacgcc ggacgcatcg tggccggatc gggggggggg
     7141 ggcgctcggt cttgccttgc tcgtcggtga tgtacttcac cagctccgcg aagtcgctct
     7201 tcttgatgga gcgcatgggg acgtgcttgg caatcacgcg caccccccgg ccgttttagc
     7261 ggctaaaaaa gtcatggctc tgccctcggg cggaccacgc ccatcatgac cttgccaagc
     7321 tcgtcctgct tctcttcgat cttcgccagc agggcgagga tcgtggcatc accgaaccgc
     7381 gccgtgcgcg ggtcgtcggt gagccagagt ttcagcaggc cgcccaggcg gcccaggtcg
     7441 ccattgatgc gggccagctc gcggacgtgc tcatagtcca cgacgcccgt gattttgtag
     7501 ccctggccga cggccagcag gtaggccgac aggctcatgc cggccgccgc cgccttttcc
     7561 tcaatcgctc ttcgttcgtc tggaaggcag tacaccttga taggtgggct gcccttcctg
     7621 gttggcttgg tttcatcagc catccgcttg ccctcatctg ttacgccggc ggtagccggc
     7681 cagcctcgca gagcaggatt cccgttgagc accgccaggt gcgaataagg gacagtgaag
     7741 aaggaacacc cgctcgcggg tgggcctact tcacctatcc tgcccggctg acgccgttgg
     7801 atacaccaag gaaagtctac acgaaccctt tggcaaaatc ctgtatatcg tgcgaaaaag
     7861 gatggatata ccgaaaaaat cgctataatg accccgaagc agggttatgc agcggaaaag
     7921 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     7981 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     8041 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     8101 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     8161 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     8221 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     8281 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaa
//
