LOCUS       Exported               12095 bp ds-DNA     circular SYN 06-SEP-2024
DEFINITION  Gibson/Homology Cloning ligation product.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12095)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Apr 11, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12095
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          4185..9980
                     /organism="synthetic DNA construct"
     misc_feature    1..4115
                     /label=EML002 backbone (fEL077+fEL076) extraction (con...
     misc_feature    1..4115
                     /label=Extracted region from EML002
     CDS             complement(171..1031)
                     /label=AmpR
     promoter        complement(1032..1136)
                     /label=AmpR promoter
     primer_bind     1176..1195
                     /label=oEL180
     mobile_element  complement(1210..1375)
                     /label=transposon:Tn7
     misc_feature    1475..4076
                     /label=oEL172 + oEL159 (concatenated sequence 2)
     misc_feature    1475..1504
                     /label=Ligation
     primer_bind     complement(1475..1504)
                     /label=oEL173
     primer_bind     1475..1504
                     /label=oEL172
     primer_bind     complement(1475..1504)
                     /label=oEL173
     primer_bind     1475..1504
                     /label=oEL172
     primer_bind     complement(1501..1523)
                     /label=oEL236
     CDS             1507..2322
                     /label=KanR
     primer_bind     complement(1717..1736)
                     /label=oEL179
     terminator      2701..2732
                     /label=T4-32
     primer_bind     3660..3679
                     /label=oEL178
     terminator      3756..3787
                     /label=T4-32
     promoter        complement(3891..3968)
                     /label=AmpR promoter
     primer_bind     3994..4022
                     /label=oEL245
     misc_feature    4044..4076
                     /label=Ligation
     primer_bind     complement(4049..4068)
                     /label=oEL182
     primer_bind     complement(4055..4076)
                     /label=3G_bb_R
     primer_bind     complement(4056..4076)
                     /label=oEL159
     misc_feature    4077..10123
                     /label=UNS1_A - P2m-U3d-lux part plasmid wout bsai-t0 ...
     misc_feature    4077..4120
                     /label=UNS1_A (concatenated sequence 1)
     primer_bind     4077..4116
                     /label=UNS1
     misc_feature    4077..4115
                     /label=Ligation
     misc_feature    4077..4115
                     /label=UNS1
     primer_bind     4077..4115
                     /label=UNS1_F
     primer_bind     complement(4077..4115)
                     /label=UNS1_R
     misc_feature    4116
                     /label=Extracted region from UNS1_A - P2m-U3d-lux part...
     misc_feature    4117..10083
                     /label=P2m-U3d-lux part plasmid wout bsai-t0 (concaten...
     misc_feature    4117..4159
                     /label=P2m (MAYBE) (concatenated sequence 1)
     misc_feature    4117..4159
                     /label=Editing History Insertion
     misc_feature    4117..4155
                     /label=Extracted region from P2m (MAYBE)
     misc_feature    4117..4120
                     /label=Ligation
     primer_bind     4117..4120
                     /label=Binds to A sticky end
     promoter        4121..4155
                     /label=J23119
     misc_feature    4156..4184
                     /label=U3d (concatenated sequence 2)
     misc_feature    4156..4180
                     /label=Extracted region from U3d
     misc_feature    4156..4159
                     /label=Ligation
     RBS             4160..4180
                     /label=B0034m
     5'UTR           4164..4175
                     /label=B0034
     misc_feature    4181..9984
                     /label=lux part plasmid wout bsai (concatenated sequen...
     misc_feature    4181..9980
                     /label=Extracted region from lux part plasmid wout bsai
     misc_feature    4181..4184
                     /label=Ligation
     CDS             4182..9980
                     /label=lux operon
     misc_feature    4185..9980
                     /label=Editing History Insertion
     misc_feature    4185..9980
                     /label=pJM220-T0T1::PrhaBAD-mS extraction PCR Product ...
     CDS             4185..5624
                     /gene="luxC"
                     /product="LuxC fatty acid reductase"
                     /label=LuxC
     primer_bind     complement(4761..4786)
                     /label=oOW005
     primer_bind     complement(5470..5502)
                     /label=oOW010
     primer_bind     complement(5470..5488)
                     /label=oOW010 binding region?
     primer_bind     5481..5520
                     /label=oOW009
     primer_bind     5496..5520
                     /label=oOW009 binding region?
     CDS             5637..6560
                     /codon_start=-1
                     /gene="luxD"
                     /product="LuxD acyltransferase"
                     /label=LuxD
                     /translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
                     FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
                     RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
                     PNNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
                     TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
                     FEHLTIATVNERRMRIEIENQAISLS"
     CDS             6609..7691
                     /codon_start=-1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             7706..8689
                     /codon_start=-1
                     /gene="luxB"
                     /product="LuxB luciferase subunit"
                     /label=LuxB
                     /translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
                     FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILG
                     FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
                     TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI
                     DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT
                     AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMEYT"
     CDS             8868..9980
                     /codon_start=-1
                     /gene="luxE"
                     /product="LuxE"
                     /label=LuxE
                     /translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
                     RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
                     NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
                     VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
                     GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
                     MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
                     YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
     primer_bind     8868..8901
                     /label=lux_F
     primer_bind     complement(9959..9980)
                     /label=lux_R
     misc_feature    9981..10083
                     /label=DVLA backbone w terminator overhangs - EML001 w...
     misc_feature    9981..10083
                     /label=EML001 with UNS - lambda t0 terminator (concate...
     misc_feature    9981..9984
                     /label=Ligation
     misc_feature    9981..9984
                     /label=Ligation
     misc_feature    9981..9984
                     /label=Extracted region from T2m
     terminator      complement(9985..10079)
                     /label=lambda t0 terminator
     misc_feature    9985..10079
                     /label=Extracted region from EML001 with UNS
     primer_bind     9985..10006
                     /label=EML001 with UNS - lambda t0 terminator (1 F) - ...
     primer_bind     complement(10056..10079)
                     /label=EML001 with UNS - lambda t0 terminator (95 R) -...
     misc_feature    join(10080..10083,9981..9984)
                     /label=DVLA backbone w terminator overhangs (concatena...
     misc_feature    10080..10123
                     /label=UNSX_E (concatenated sequence 3)
     misc_feature    10080..10123
                     /label=Extracted region from UNSX_E
     misc_feature    10080..10083
                     /label=Ligation
     misc_feature    10080..10083
                     /label=Ligation
     primer_bind     10080..10083
                     /label=Binds to E sticky end
     misc_feature    join(10084..10153,4044..4115)
                     /label=fEL042 (concatenated sequence 3)
     misc_feature    10084..12095
                     /label=EML002 backbone (fEL077+fEL076) extraction (con...
     misc_feature    10084..10123
                     /label=Ligation
     primer_bind     10084..10123
                     /label=UNS10
     misc_feature    10084..10123
                     /label=UNSX
     primer_bind     10084..10123
                     /label=UNSX_F
     misc_feature    10124..13599
                     /label=oEL171 + oEL173 (concatenated sequence 1)
     misc_feature    10124..10153
                     /label=Extracted region from fEL042
     misc_feature    10124..10153
                     /label=Ligation
     primer_bind     10124..10145
                     /label=oEL171
     misc_feature    10154..12095
                     /label=Extracted region from EML001 with UNS
     mobile_element  complement(10204..10402)
                     /label=transposon:Tn7
     primer_bind     complement(10411..10432)
                     /label=oEL188
     primer_bind     10412..10431
                     /label=oEL181
     rep_origin      complement(10484..10872)
                     /direction=LEFT
                     /label=R6K gamma ori
     CDS             10869..10898
                     /label=TcR
     CDS             complement(11043..11414)
                     /label=traJ
     oriT            complement(11447..11556)
                     /direction=LEFT
     rep_origin      complement(11690..12095)
                     /direction=LEFT
                     /label=ori
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc tgaattaatt cgagctcgcc caaacataac aggaagaaaa
     1201 atgccccgct gtgggcggac aaaatagttg ggaactggga ggggtggaaa tggagttttt
     1261 aaggattatt tagggaagag tgacaaaata gatgggaact gggtgtagcg tcgtaagcta
     1321 atacgaaaat taaaaatgac aaaatagttt ggaactagat ttcacttatc tggttggcct
     1381 gcaggggggg ggggggaaag ccacgttgtg tctcaaaatc tctgatgtta cattgcacaa
     1441 gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg
     1501 ggtgttatga gccatattca acgggaaacg tcttgctcga ggccgcgatt aaattccaac
     1561 atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg
     1621 acaatctatc gattgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa
     1681 ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt
     1741 atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc
     1801 actgcgatcc ccgggaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa
     1861 aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat
     1921 tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac
     1981 ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc
     2041 tggaaagaaa tgcataagct tttgccattc tcaccggatt cagtcgtcac tcatggtgat
     2101 ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga
     2161 cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag
     2221 ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg
     2281 aataaattgc agtttcattt gatgctcgat gagtttttct aatcagaatt ggttaattgg
     2341 ttgtaacact ggcagagcat tacgctgact tgacgggacg gcggctttgt tgaataaatc
     2401 gaacttttgc tgagttgaag gatcagatca cgcatcttcc cgacaacgca gaccgttccg
     2461 tggcaaagca aaagttcaaa atcaccaact ggtccaccta caacaaagct ctcatcaacc
     2521 gtggctccct cactttctgg ctggatgatg gggcgattca ggcctggtat gagtcagcaa
     2581 caccttcttc acgaggcaga cctcagcgcc cccccccccc tgcaggtcga ctagagtcgg
     2641 ggatccggtg attgattgag caagctttat gcttgtaaac cgttttgtga aaaaattttt
     2701 aaaataaaaa aggggacctc tagggtcccc aattaattag taatataatc tattaaaggt
     2761 cattcaaaag gtcatccacc ggatcaattc ccctgctcgc gcaggctggg tgccaagctc
     2821 tcgggtaaca tcaaggcccg atccttggag cccttgccct cccgcacgat gatcgtgccg
     2881 tgatcgaaat ccagatcctt gacccgcagt tgcaaaccct cactgatccg catgcccgtt
     2941 ccatacagaa gctgggcgaa caaacgatgc tcgccttcca gaaaaccgag gatgcgaacc
     3001 acttcatccg gggtcagcac caccggcaag cgccgcgacg gccgaggtct tccgatctcc
     3061 tgaagccagg gcagatccgt gcacagcacc ttgccgtaga agaacagcaa ggccgccaat
     3121 gcctgacgat gcgtggagac cgaaaccttg cgctcgttcg ccagccagga cagaaatgcc
     3181 tcgacttcgc tgctgcccaa ggttgccggg tgacgcacac cgtggaaacg gatgaaggca
     3241 cgaacccagt ggacataagc ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc
     3301 tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt
     3361 ttcatggctt gttatgactg tttttttggg gtacagtcta tgcctcgggc atccaagcag
     3421 caagcgcgtt acgccgtggg tcgatgtttg tgtctaacaa ttcgttcaag ccgacgccgc
     3481 ttcgcggcgc ggcttaactc aagcgttaga tgcactaagc acataattgc tcacagccaa
     3541 actatcaggt caagtctgct tttattattt ttaagcgtgc ataataagcc ctacacaaat
     3601 tgggagatat atcatgaaag gctggctttt tcttgttatc gcaatagttg gcgaagtaat
     3661 cgcaacatcc gcattaaaat ctagcgaggg ctttactaag ctgatccggt ggatgacctt
     3721 ttgaatgacc tttaatagat tatattacta attaattggg gaccctagag gtcccctttt
     3781 ttattttaaa aattttttca caaaacggtt tacaagcata aagcttgctc aatcaatcac
     3841 cggatcccga cgggcccggt accgcggccg cgcgaattcg agctcggtac catttatcag
     3901 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     3961 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     4021 acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcacct cgagaacatt
     4081 actcgcatcc attctcaggc tgtctcgtct cgtctcggag ttgacggcta gctcagtcct
     4141 aggtacagtg ctagctacta gagaaagagg agaaatacta aatgactaaa aaaatttcat
     4201 tcattattaa cggccaggtt gaaatctttc ccgaaggtga tgatttagtg caatccatta
     4261 attttggtga taatagtgtt tacctgccaa tattgaatga ctctcatgta aaaaacatta
     4321 ttgattgtaa tggaaataac gaattacggt tgcataacat tgtcaatttt ctctatacgg
     4381 tagggcaaag atggaaaaat gaagaatact caagacgcag gacatacatt cgtgacttaa
     4441 aaaaatatat gggatattca gaagaaatgg ctaagctaga ggccaattgg atatctatga
     4501 ttttatgttc taaaggcggc ctttatgatg ttgtagaaaa tgaacttggt tctcgccata
     4561 tcatggatga atggctacct caggatgaaa gttatgttcg ggcttttccg aaaggtaaat
     4621 ctgtacatct gttggcaggt aatgttccat tatctgggat catgtctata ttacgcgcaa
     4681 ttttaactaa gaatcagtgt attataaaaa catcgtcaac cgatcctttt accgctaatg
     4741 cattagcgtt aagttttatt gatgtagacc ctaatcatcc gataacgcgc tctttatctg
     4801 ttatatattg gccccaccaa ggtgatacat cactcgcaaa agaaattatg caacatgcgg
     4861 atgttattgt cgcttgggga gggccagatg cgattaattg ggcggtagag catgcgccat
     4921 cttatgctga tgtgattaaa tttggttcta aaaagagtct ttgcattatc gataatcctg
     4981 ttgatttgac gtccgcagcg acaggtgcgg ctcatgatgt ttgtttttac gatcagcgag
     5041 cttgtttttc tgcccaaaac atatattaca tgggaaatca ttatgaggaa tttaagttag
     5101 cgttgataga aaaacttaat ctatatgcgc atatattacc gaatgccaaa aaagattttg
     5161 atgaaaaggc ggcctattct ttagttcaaa aagaaagctt gtttgctgga ttaaaagtag
     5221 aggtggatat tcatcaacgt tggatgatta ttgagtcaaa tgcaggtgtg gaatttaatc
     5281 aaccacttgg cagatgtgtg taccttcatc acgtcgataa tattgagcaa atattgcctt
     5341 atgttcaaaa aaataagacg caaaccatat ctatttttcc ttgggagtca tcatttaaat
     5401 atcgagatgc gttagcatta aaaggtgcgg aaaggattgt agaagcagga atgaataaca
     5461 tatttcgagt tggtggatct catgacggga tgaggccgtt gcaacgatta gtgacatata
     5521 tttctcatga aaggccatct aactatacgg ctaaggatgt tgcggttgaa atagaacaga
     5581 ctcgattcct ggaagaagat aagttccttg tatttgtccc ataataggta aaaagtatgg
     5641 aaaatgaatc aaaatataaa accatcgacc acgttatttg tgttgaagga aataaaaaaa
     5701 ttcatgtttg ggaaacgctg ccagaagaaa acagcccaaa gagaaagaat gccattatta
     5761 ttgcgtctgg ttttgcccgc aggatggatc attttgctgg tctggcggaa tatttatcgc
     5821 ggaatggatt tcatgtgatc cgctatgatt cgcttcacca cgttggattg agttcaggga
     5881 caattgatga atttacaatg tctataggaa agcagagctt gttagcagtg gttgattggt
     5941 taactacacg aaaaataaat aacttcggta tgttggcttc aagcttatct gcgcggatag
     6001 cttatgcaag cctatctgaa atcaatgctt cgtttttaat caccgcagtc ggtgttgtta
     6061 acttaagata ttctcttgaa agagctttag ggtttgatta tctcagtcta cccattaatg
     6121 aattgccgaa taatctagat tttgaaggcc ataaattggg tgctgaagtc tttgcgagag
     6181 attgtcttga ttttggttgg gaagatttag cttctacaat taataacatg atgtatcttg
     6241 atataccgtt tattgctttt actgcaaata acgataattg ggtcaagcaa gatgaagtta
     6301 tcacattgtt atcaaatatt cgtagtaatc gatgcaagat atattctttg ttaggaagtt
     6361 cgcatgactt gagtgaaaat ttagtggtcc tgcgcaattt ttatcaatcg gttacgaaag
     6421 ccgctatcgc gatggataat gatcatctgg atattgatgt tgatattact gaaccgtcat
     6481 ttgaacattt aactattgcg acagtcaatg aacgccgaat gagaattgag attgaaaatc
     6541 aagcaatttc tctgtcttaa aatctattga gatattctat cactcaaata gcaatataag
     6601 gactctctat gaaatttgga aactttttgc ttacatacca acctccccaa ttttctcaaa
     6661 cagaggtaat gaaacgtttg gttaaattag gtcgcatctc tgaggagtgt ggttttgata
     6721 ccgtatggtt actggagcat catttcacgg agtttggttt gcttggtaac ccttatgtcg
     6781 ctgctgcata tttacttggc gcgactaaaa aattgaatgt aggaactgcc gctattgttc
     6841 ttcccacagc ccatccagta cgccaacttg aagatgtgaa tttattggat caaatgtcaa
     6901 aaggacgatt tcggtttggt atttgccgag ggctttacaa caaggacttt cgcgtattcg
     6961 gcacagatat gaataacagt cgcgccttag cggaatgctg gtacgggctg ataaagaatg
     7021 gcatgacaga gggatatatg gaagctgata atgaacatat caagttccat aaggtaaaag
     7081 taaaccccgc ggcgtatagc agaggtggcg caccggttta tgtggtggct gaatcagctt
     7141 cgacgactga gtgggctgct caatttggcc taccgatgat attaagttgg attataaata
     7201 ctaacgaaaa gaaagcacaa cttgagcttt ataatgaagt ggctcaagaa tatgggcacg
     7261 atattcataa tatcgaccat tgcttatcat atataacatc tgtagatcat gactcaatta
     7321 aagcgaaaga gatttgccgg aaatttctgg ggcattggta tgattcttat gtgaatgcta
     7381 cgactatttt tgatgattca gaccaaacaa gaggttatga tttcaataaa gggcagtggc
     7441 gtgactttgt attaaaagga cataaagata ctaatcgccg tattgattac agttacgaaa
     7501 tcaatcccgt gggaacgccg caggaatgta ttgacataat tcaaaaagac attgatgcta
     7561 caggaatatc aaatatttgt tgtggatttg aagctaatgg aacagtagac gaaattattg
     7621 cttccatgaa gctcttccag tctgatgtca tgccatttct taaagaaaaa caacgttcgc
     7681 tattatatta gctaaggaga aagaaatgaa atttggattg ttcttcctta acttcatcaa
     7741 ttcaacaact gttcaagaac aaagtatagt tcgcatgcag gaaataacgg agtatgttga
     7801 taagttgaat tttgaacaga ttttagtgta tgaaaatcat ttttcagata atggtgttgt
     7861 cggcgctcct ctgactgttt ctggttttct gctcggttta acagagaaaa ttaaaattgg
     7921 ttcattaaat cacatcatta caactcatca tcctgtccgc atagcggagg aagcttgctt
     7981 attggatcag ttaagtgaag ggagatttat tttagggttt agtgattgcg aaaaaaaaga
     8041 tgaaatgcat ttttttaatc gcccggttga atatcaacag caactatttg aagagtgtta
     8101 tgaaatcatt aacgatgctt taacaacagg ctattgtaat ccagataacg atttttatag
     8161 cttccctaaa atatctgtaa atccccatgc ttatacgcca ggcggacctc ggaaatatgt
     8221 aacagcaacc agtcatcata ttgttgagtg ggcggccaaa aaaggtattc ctctcatctt
     8281 taagtgggat gattctaatg atgttagata tgaatatgct gaaagatata aagccgttgc
     8341 ggataaatat gacgttgacc tatcagagat agaccatcag ttaatgatat tagttaacta
     8401 taacgaagat agtaataaag ctaaacaaga gacgcgtgca tttattagtg attatgttct
     8461 tgaaatgcac cctaatgaaa atttcgaaaa taaacttgaa gaaataattg cagaaaacgc
     8521 tgtcggaaat tatacggagt gtataactgc ggctaagttg gcaattgaaa agtgtggtgc
     8581 gaaaagtgta ttgctgtcct ttgaaccaat gaatgatttg atgagccaaa aaaatgtaat
     8641 caatattgtt gatgataata ttaagaagta ccacatggaa tatacctaat agatttcgag
     8701 ttgcagcgag gcggcaagtg aacgaatccc caggagcata gataactatg tgactggggt
     8761 gagtgaaagc agccaacaaa gcagcagctt gaaagatgaa gggtataaaa gagtatgaca
     8821 gcagtgctgc catactttct aatattatct tgaggagtaa aacaggtatg acttcatatg
     8881 ttgataaaca agaaattaca gcaagctcag aaattgatga tttgattttt tcgagcgatc
     8941 cattagtgtg gtcttacgac gagcaggaaa aaatcagaaa gaaacttgtg cttgatgcat
     9001 ttcgtaatca ttataaacat tgtcgagaat atcgtcacta ctgtcaggca cacaaagtag
     9061 atgacaatat tacggaaatt gatgacatac ctgtattccc aacatcggtt tttaagttta
     9121 ctcgcttatt aacttctcag gaaaacgaga ttgaaagttg gtttaccagt agcggcacga
     9181 atggtttaaa aagtcaggtg gcgcgtgaca gattaagtat tgagagactc ttaggctctg
     9241 tgagttatgg catgaaatat gttggtagtt ggtttgatca tcaaatagaa ttagtcaatt
     9301 tgggaccaga tagatttaat gctcataata tttggtttaa atatgttatg agtttggtgg
     9361 aattgttata tcctacgaca tttaccgtaa cagaagaacg aatagatttt gttaaaacat
     9421 tgaatagtct tgaacgaata aaaaatcaag ggaaagatct ttgtcttatt ggttcgccat
     9481 actttattta tttactctgc cattatatga aagataaaaa aatctcattt tctggagata
     9541 aaagccttta tatcataacc ggaggcggct ggaaaagtta cgaaaaagaa tctctgaaac
     9601 gtgatgattt caatcatctt ttatttgata ctttcaatct cagtgatatt agtcagatcc
     9661 gagatatatt taatcaagtt gaactcaaca cttgtttctt tgaggatgaa atgcagcgta
     9721 aacatgttcc gccgtgggta tatgcgcgag cgcttgatcc tgaaacgttg aaacctgtac
     9781 ctgatggaac gccggggttg atgagttata tggatgcgtc agcaaccagt tatccagcat
     9841 ttattgttac cgatgatgtc gggataatta gcagagaata tggtaagtat cccggcgtgc
     9901 tcgttgaaat tttacgtcgc gtcaatacga ggacgcagaa agggtgtgct ttaagcttaa
     9961 ccgaagcgtt tgatagttga aggtgactcc tgttgataga tccagtaatg acctcagaac
    10021 tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatt ggtgagaatg
    10081 cttccaggat acatagatta ccacaactcc gagcccttcc accccaagct agcttggagg
    10141 acaaatccgc cgctaggagc ttgcggccgc cttaagacgc gtggatcccc gggaatttcg
    10201 accccacgcc cctctttaat acgacgggca atttgcactt cagaaaatga agagtttgct
    10261 ttagccataa caaaagtcca gtatgctttt tcacagcata actggactga tttcagttta
    10321 caactattct gtctagttta agactttatt gtcatagttt agatctattt tgttcagttt
    10381 aagactttat tgtccgccca cacccgctta cgcagggcat ccatttatta ctcaaccgta
    10441 accgattttg ccaggttacg cggctggtcc tctagaattc ccatgtcagc cgttaagtgt
    10501 tcctgtgtca ctcaaaattg ctttgagagg ctctaagggc ttctcagtgc gttacatccc
    10561 tggcttgttg tccacaaccg ttaaacctta aaagctttaa aagccttata tattcttttt
    10621 tttcttataa aacttaaaac cttagaggct atttaagttg ctgatttata ttaattttat
    10681 tgttcaaaca tgagagctta gtacgtgaaa catgagagct tagtacgtta gccatgagag
    10741 cttagtacgt tagccatgag ggtttagttc gttaaacatg agagcttagt acgttaaaca
    10801 tgagagctta gtacgtgaaa catgagagct tagtacgtac tatcaacagg ttgaactgct
    10861 gatcttcaga tcctctacgc cggacgcatc gtggccggat cggggggggg gggcgctcgg
    10921 tcttgccttg ctcgtcggtg atgtacttca ccagctccgc gaagtcgctc ttcttgatgg
    10981 agcgcatggg gacgtgcttg gcaatcacgc gcaccccccg gccgttttag cggctaaaaa
    11041 agtcatggct ctgccctcgg gcggaccacg cccatcatga ccttgccaag ctcgtcctgc
    11101 ttctcttcga tcttcgccag cagggcgagg atcgtggcat caccgaaccg cgccgtgcgc
    11161 gggtcgtcgg tgagccagag tttcagcagg ccgcccaggc ggcccaggtc gccattgatg
    11221 cgggccagct cgcggacgtg ctcatagtcc acgacgcccg tgattttgta gccctggccg
    11281 acggccagca ggtaggccga caggctcatg ccggccgccg ccgccttttc ctcaatcgct
    11341 cttcgttcgt ctggaaggca gtacaccttg ataggtgggc tgcccttcct ggttggcttg
    11401 gtttcatcag ccatccgctt gccctcatct gttacgccgg cggtagccgg ccagcctcgc
    11461 agagcaggat tcccgttgag caccgccagg tgcgaataag ggacagtgaa gaaggaacac
    11521 ccgctcgcgg gtgggcctac ttcacctatc ctgcccggct gacgccgttg gatacaccaa
    11581 ggaaagtcta cacgaaccct ttggcaaaat cctgtatatc gtgcgaaaaa ggatggatat
    11641 accgaaaaaa tcgctataat gaccccgaag cagggttatg cagcggaaaa gcgctttctc
    11701 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
    11761 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
    11821 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
    11881 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
    11941 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
    12001 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
    12061 agcagcagat tacgcgcaga aaaaaaggat ctcaa
//
